Surviving heat shock: Control strategies for robustness and performance (2005)
El-Samad, H., Kurata, H., Doyle, J. C., Gross, C. A., Khammash, M.
Molecular biology studies the cause-and-effect relationships among microscopic processes initiated by individual molecules within a cell and observes their macroscopic phenotypic effects on cells and...
The sigma subunit of Escherichia coli RNA polymerase senses promoter spacing.
Dombroski, A J, Johnson, B D, Lonetto, M, Gross, C A
The promoters recognized by sigma 70, the primary sigma of Escherichia coli, consist of two highly conserved hexamers located at -10 and -35 bp from the start point of transcription, separated by a...
Jin, D J, Burgess, R R, Richardson, J P, Gross, C A
Rho-dependent terminators constitute one of two major classes of terminators in Escherichia coli. Termination at these sites requires the concerted action of RNA polymerase and rho protein. We...
Wild, J, Kamath-Loeb, A, Ziegelhoffer, E, Lonetto, M, Kawasaki, Y, Gross, C A
A set of 37 mutations in DnaK, the Escherichia coli homologue of the 70-kDa heat shock proteins, was isolated using a selection for high constitutive expression of heat shock proteins. Of these, 11...
Mecsas, J, Welch, R, Erickson, J W, Gross, C A
We previously reported that a region of the Escherichia coli chromosome at 18 min increased E sigma E activity when cloned in multicopy (J. Mecsas, P. E. Rouviere, J. W. Erickson, T. J. Donohue, and...
Involvement of the DnaK-DnaJ-GrpE chaperone team in protein secretion in Escherichia coli.
Wild, J, Rossmeissl, P, Walter, W A, Gross, C A
We used depletion studies designed to further investigate the role of the DnaK, DnaJ, and GrpE heat shock proteins in the SecB-dependent and SecB-independent secretion pathways. Our previous finding...
Tavormina, P L, Landick, R, Gross, C A
The beta subunit of prokaryotic RNA polymerase shares significant sequence similarity with its eukaryotic and archaeal counterparts across most of the protein. Nine segments of particularly high...
SigmaE is an essential sigma factor in Escherichia coli.
SigmaE is an alternative sigma factor that controls the extracytoplasmic stress response in Escherichia coli. SigmaE is essential at high temperatures but was previously thought to be nonessential at...
Accumulation of secretory protein precursors in Escherichia coli induces the heat shock response.
Wild, J, Walter, W A, Gross, C A, Altman, E
The accumulation of secretory protein precursors, caused either by mutations in secB or secA or by the overproduction of export-defective proteins, results in a two- to fivefold increase in the...
Zhou, Y N, Walter, W A, Gross, C A
sigma 70, encoded by rpoD, is the major sigma factor in Escherichia coli. rpoD285 (rpoD800) is a small deletion mutation in rpoD that confers a temperature-sensitive growth phenotype because the...
In Escherichia coli, transcription of the heat shock genes is regulated by sigma 32, the alternative sigma factor directing RNA polymerase to heat shock promoters. sigma 32, encoded by rpoH (htpR),...
DnaK, DnaJ, and GrpE are required for flagellum synthesis in Escherichia coli.
Shi, W, Zhou, Y, Wild, J, Adler, J, Gross, C A
The DnaK, DnaJ, and GrpE heat shock proteins are required for motility of Escherichia coli. Cells deleted for dnaK or dnaJ, or with some mutations in the dnaK or grpE gene, are nonmotile, lack...
Translational regulation of sigma 32 synthesis: requirement for an internal control element.
We have investigated the sequence requirements for the translational regulation of sigma 32 by examining the behavior of a new rpoH-lacZ protein fusion containing a short N-terminal fragment of sigma...
Singer, M, Rossmiessl, P, Cali, B M, Liebke, H, Gross, C A
The ts8 mutant of Escherichia coli has previously been shown to preferentially inhibit stable RNA synthesis when shifted to the nonpermissive temperature. We demonstrate in this report that the ts8...
Singer, M, Walter, W A, Cali, B M, Rouviere, P, Liebke, H H, Gourse, R L, ...
The conditional lethal mutations ts8 and h8 are located in fda, the gene encoding aldolase, and they inhibit RNA synthesis upon shift to the nonpermissive temperature. We demonstrate that both...
We used our collection of 17 sequenced rifampin resistance alleles in rpoB to perform a systematic analysis of the phenotypes historically reported with this class of mutants, including growth...
Zhou, Y N, Kusukawa, N, Erickson, J W, Gross, C A, Yura, T
The product of the Escherichia coli rpoH (htpR) gene, sigma 32, is required for heat-inducible transcription of the heat shock genes. Previous studies on the role of sigma 32 in growth at low...
Grossman, A D, Ullmann, A, Burgess, R R, Gross, C A
An immediate 12-fold inhibition in the rate of beta-galactosidase synthesis occurs in Escherichia coli cells containing the mutant sigma allele rpoD800 after a shift to 42 degrees C. In the present...
Burton, Z, Burgess, R R, Lin, J, Moore, D, Holder, S, Gross, C A
We have determined the nucleotide sequence of the rpoD gene which codes for the sigma subunit of RNA polymerase from E. coli K12. The gene, which we formerly cloned as a HindIII restriction fragment...
Singer, M, Baker, T A, Schnitzler, G, Deischel, S M, Goel, M, Dove, W, ...
We present a collection of 182 isogenic strains containing genetically linked antibiotic resistance elements located at approximately 1-min intervals around the Escherichia coli chromosome. At most...
A gene regulating the heat shock response in Escherichia coli also affects proteolysis.
Baker, T A, Grossman, A D, Gross, C A
The htpR locus in Escherichia coli encodes a regulator of the heat shock response. Cells containing the htpR165 mutation are defective in the induction of synthesis of heat-shock proteins at high...
Consensus sequence for Escherichia coli heat shock gene promoters.
Cowing, D W, Bardwell, J C, Craig, E A, Woolford, C, Hendrix, R W, Gross, C A
We have identified promoters for the Escherichia coli heat shock operons dnaK and groE and the gene encoding heat shock protein C62.5. Transcription from each promoter is heat-inducible in vivo, and...
rpoE, the gene encoding the second heat-shock sigma factor, sigma E, in Escherichia coli.
Rouvière, P E, Mecsas, J, Lu, C Z, Rudd, K E, Gross, C A
In Escherichia coli, the heat shock response is under the control of two alternative sigma factors: sigma 32 and sigma E. The sigma 32-regulated response is well understood, whereas little is known...
Gross, C A, Blattner, F R, Taylor, W E, Lowe, P A, Burgess, R R
A transducing phage has been isolated with codes for the sigma subunit of Escherichia coli RNA polymerase. Transducing phage were selected from E. coli shotgun collections of HindIII or Sac I...
Surviving heat shock: Control strategies for robustness and performance
El-Samad, H., Kurata, H., Doyle, J. C., Gross, C. A., Khammash, M.
Molecular biology studies the cause-and-effect relationships among microscopic processes initiated by individual molecules within a cell and observes their macroscopic phenotypic effects on cells and...
Mu dX, a derivative of Mu d1 (lac Apr) which makes stable lacZ fusions at high temperature.
Baker, T A, Howe, M M, Gross, C A
We describe defective Mu phage Mu dX (Mu d1 Bx::Tn9 [lac Apr Cmr]) which is useful for insertion mutagenesis and for construction of lac operon fusions in vivo. Mu dX retains the insertion properties...
The sigma subunit of Escherichia coli RNA polymerase senses promoter spacing.
Dombroski, A J, Johnson, B D, Lonetto, M, Gross, C A
The promoters recognized by sigma 70, the primary sigma of Escherichia coli, consist of two highly conserved hexamers located at -10 and -35 bp from the start point of transcription, separated by a...
Jin, D J, Burgess, R R, Richardson, J P, Gross, C A
Rho-dependent terminators constitute one of two major classes of terminators in Escherichia coli. Termination at these sites requires the concerted action of RNA polymerase and rho protein. We...
Wild, J, Kamath-Loeb, A, Ziegelhoffer, E, Lonetto, M, Kawasaki, Y, Gross, C A
A set of 37 mutations in DnaK, the Escherichia coli homologue of the 70-kDa heat shock proteins, was isolated using a selection for high constitutive expression of heat shock proteins. Of these, 11...
Mecsas, J, Welch, R, Erickson, J W, Gross, C A
We previously reported that a region of the Escherichia coli chromosome at 18 min increased E sigma E activity when cloned in multicopy (J. Mecsas, P. E. Rouviere, J. W. Erickson, T. J. Donohue, and...
Involvement of the DnaK-DnaJ-GrpE chaperone team in protein secretion in Escherichia coli.
Wild, J, Rossmeissl, P, Walter, W A, Gross, C A
We used depletion studies designed to further investigate the role of the DnaK, DnaJ, and GrpE heat shock proteins in the SecB-dependent and SecB-independent secretion pathways. Our previous finding...
Tavormina, P L, Landick, R, Gross, C A
The beta subunit of prokaryotic RNA polymerase shares significant sequence similarity with its eukaryotic and archaeal counterparts across most of the protein. Nine segments of particularly high...
SigmaE is an essential sigma factor in Escherichia coli.
SigmaE is an alternative sigma factor that controls the extracytoplasmic stress response in Escherichia coli. SigmaE is essential at high temperatures but was previously thought to be nonessential at...
Accumulation of secretory protein precursors in Escherichia coli induces the heat shock response.
Wild, J, Walter, W A, Gross, C A, Altman, E
The accumulation of secretory protein precursors, caused either by mutations in secB or secA or by the overproduction of export-defective proteins, results in a two- to fivefold increase in the...
Zhou, Y N, Walter, W A, Gross, C A
sigma 70, encoded by rpoD, is the major sigma factor in Escherichia coli. rpoD285 (rpoD800) is a small deletion mutation in rpoD that confers a temperature-sensitive growth phenotype because the...
In Escherichia coli, transcription of the heat shock genes is regulated by sigma 32, the alternative sigma factor directing RNA polymerase to heat shock promoters. sigma 32, encoded by rpoH (htpR),...
DnaK, DnaJ, and GrpE are required for flagellum synthesis in Escherichia coli.
Shi, W, Zhou, Y, Wild, J, Adler, J, Gross, C A
The DnaK, DnaJ, and GrpE heat shock proteins are required for motility of Escherichia coli. Cells deleted for dnaK or dnaJ, or with some mutations in the dnaK or grpE gene, are nonmotile, lack...
Translational regulation of sigma 32 synthesis: requirement for an internal control element.
We have investigated the sequence requirements for the translational regulation of sigma 32 by examining the behavior of a new rpoH-lacZ protein fusion containing a short N-terminal fragment of sigma...
Singer, M, Rossmiessl, P, Cali, B M, Liebke, H, Gross, C A
The ts8 mutant of Escherichia coli has previously been shown to preferentially inhibit stable RNA synthesis when shifted to the nonpermissive temperature. We demonstrate in this report that the ts8...
Singer, M, Walter, W A, Cali, B M, Rouviere, P, Liebke, H H, Gourse, R L, ...
The conditional lethal mutations ts8 and h8 are located in fda, the gene encoding aldolase, and they inhibit RNA synthesis upon shift to the nonpermissive temperature. We demonstrate that both...
We used our collection of 17 sequenced rifampin resistance alleles in rpoB to perform a systematic analysis of the phenotypes historically reported with this class of mutants, including growth...
Zhou, Y N, Kusukawa, N, Erickson, J W, Gross, C A, Yura, T
The product of the Escherichia coli rpoH (htpR) gene, sigma 32, is required for heat-inducible transcription of the heat shock genes. Previous studies on the role of sigma 32 in growth at low...
Grossman, A D, Ullmann, A, Burgess, R R, Gross, C A
An immediate 12-fold inhibition in the rate of beta-galactosidase synthesis occurs in Escherichia coli cells containing the mutant sigma allele rpoD800 after a shift to 42 degrees C. In the present...
Mu dX, a derivative of Mu d1 (lac Apr) which makes stable lacZ fusions at high temperature.
Baker, T A, Howe, M M, Gross, C A
We describe defective Mu phage Mu dX (Mu d1 Bx::Tn9 [lac Apr Cmr]) which is useful for insertion mutagenesis and for construction of lac operon fusions in vivo. Mu dX retains the insertion properties...
Burton, Z, Burgess, R R, Lin, J, Moore, D, Holder, S, Gross, C A
We have determined the nucleotide sequence of the rpoD gene which codes for the sigma subunit of RNA polymerase from E. coli K12. The gene, which we formerly cloned as a HindIII restriction fragment...
Singer, M, Baker, T A, Schnitzler, G, Deischel, S M, Goel, M, Dove, W, ...
We present a collection of 182 isogenic strains containing genetically linked antibiotic resistance elements located at approximately 1-min intervals around the Escherichia coli chromosome. At most...
A gene regulating the heat shock response in Escherichia coli also affects proteolysis.
Baker, T A, Grossman, A D, Gross, C A
The htpR locus in Escherichia coli encodes a regulator of the heat shock response. Cells containing the htpR165 mutation are defective in the induction of synthesis of heat-shock proteins at high...
Consensus sequence for Escherichia coli heat shock gene promoters.
Cowing, D W, Bardwell, J C, Craig, E A, Woolford, C, Hendrix, R W, Gross, C A
We have identified promoters for the Escherichia coli heat shock operons dnaK and groE and the gene encoding heat shock protein C62.5. Transcription from each promoter is heat-inducible in vivo, and...
rpoE, the gene encoding the second heat-shock sigma factor, sigma E, in Escherichia coli.
Rouvière, P E, Mecsas, J, Lu, C Z, Rudd, K E, Gross, C A
In Escherichia coli, the heat shock response is under the control of two alternative sigma factors: sigma 32 and sigma E. The sigma 32-regulated response is well understood, whereas little is known...
Gross, C A, Blattner, F R, Taylor, W E, Lowe, P A, Burgess, R R
A transducing phage has been isolated with codes for the sigma subunit of Escherichia coli RNA polymerase. Transducing phage were selected from E. coli shotgun collections of HindIII or Sac I...
Surviving heat shock: Control strategies for robustness and performance
El-Samad, H., Kurata, H., Doyle, J. C., Gross, C. A., Khammash, M.
Molecular biology studies the cause-and-effect relationships among microscopic processes initiated by individual molecules within a cell and observes their macroscopic phenotypic effects on cells and...