C. Abell

Publication List Details

Period

1986 - 2008

Number

44

Co-Authors

Nanomechanical detection of antibiotic-mucopeptide binding in a model for superbug drug resistance (2008)

Ndieyira, J. W., Watari, M., Barrera, A. Donoso, Zhou, D., Vögtli, M., Batchelor, M., ...

The alarming growth of the antibiotic-resistant superbugs methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococcus (VRE) is driving the development of new technologies...

Towards engineering increased pantothenate (Vitamin B5) levels in plants (2008)

Chakauya, E, Coxon, KM, Wei, M, MacDonald, MV, Barsby, T, Abell, C, ...

Author Posting. Copyright Springer Verlag, 2008. This is the author's version of the work. It is posted here by permission of Springer Verlag for personal use, not for redistribution

Single-step biocompatible coating for sulfhydryl coupling of receptors using 2-(pyridinyldithio)ethylcarbamoyl dextran (2008)

Li, Xin, Abell, C, Cooper, MA

2-(Pyridinyldithio)ethylcarbamoyl dextran (PDEC dextran) is developed herein as a novel biosensor coating material aimed for direct and facile fabrication of a sulfhydryl-specific capture surface on...

Design and synthesis of aromatic inhibitors of anthranilate synthase (2005)

Payne, R. J., Bulloch, E. M. M., Abell, Andrew D., Abell, C.

Anthranilate synthase catalyses the conversion of chorismate to anthranilate, a key step in tryptophan biosynthesis. A series of 3-(1-carboxy-ethoxy) benzoic acids were synthesised as chorismate...

Inhibition studies on salicylate synthase (2005)

Payne, R. J., Kerbarh, O., Miguel, R. N., Abell, Andrew David, Abell, C.

Analogues of chorismate and isochorismate were designed and tested as potential inhibitors in the first inhibition study against a salicylate synthase.

Design and synthesis of aromatic inhibitors of anthranilate synthase (2005)

Payne, R. J., Bulloch, E. M. M., Abell, Andrew D., Abell, C.

Anthranilate synthase catalyses the conversion of chorismate to anthranilate, a key step in tryptophan biosynthesis. A series of 3-(1-carboxy-ethoxy) benzoic acids were synthesised as chorismate...

Inhibition studies on salicylate synthase (2005)

Payne, R. J., Kerbarh, O., Miguel, R. N., Abell, Andrew David, Abell, C.

Analogues of chorismate and isochorismate were designed and tested as potential inhibitors in the first inhibition study against a salicylate synthase.

A Convenient Method for the Synthesis of Dehydroquinic Acid (2003)

Le Sann, C., Abell, C., Abell, Andrew David

A convenient synthesis of dehydroquinic acid and its corresponding methyl ester are described. Protection of the trans diol of quinic acid, followed by PCC oxidation, gave fully protected...

A Convenient Method for the Synthesis of Dehydroquinic Acid (2003)

Le Sann, C., Abell, C., Abell, Andrew David

A convenient synthesis of dehydroquinic acid and its corresponding methyl ester are described. Protection of the trans diol of quinic acid, followed by PCC oxidation, gave fully protected...

Creating nanoscale patterns of dendrimers on silicon surfaces with dip-pen nanolithography (2002)

McKendry, R., Huck, W.T.S., Weeks, B., Fiorini, M., Abell, C., Rayment, T.

Dip-pen nanolithography (DPN) is a nanowriting procedure that employs an AFM tip as a "nanopen" to deposit organic molecules onto a substrate surface. This paper describes the application of DPN to...

Cloning and sequencing of the Escherichia coli panB gene, which encodes ketopantoate hydroxymethyltransferase, and overexpression of the enzyme.

Jones, C E, Brook, J M, Buck, D, Abell, C, Smith, A G

The panB gene from Escherichia coli, encoding the first enzyme of the pantothenate biosynthesis pathway, ketopantoate hydroxymethyltransferase (KPHMT), has been isolated by functional complementation...

Evidence that pyridoxal phosphate modification of lysine residues (Lys-55 and Lys-59) causes inactivation of hydroxymethylbilane synthase (porphobilinogen deaminase).

Miller, A D, Packman, L C, Hart, G J, Alefounder, P R, Abell, C, Battersby, A R

A recombinant strain of Escherichia coli has been constructed that produces approx. 200 times the amount of hydroxymethylbilane synthase found in wild-type E. coli [Hart, Abell & Battersby (1986)...

Escherichia coli chorismate synthase: a deuterium kinetic-isotope effect under single-turnover and steady-state conditions shows that a flavin intermediate forms before the C-(6proR)-H bond is cleaved.

Bornemann, S, Balasubramanian, S, Coggins, J R, Abell, C, Lowe, D J, Thorneley, R N

We report the observation of a deuterium kinetic isotope effect for the conversion of 5-enolpyruvylshikimate-3-phosphate into chorismate (6proR2HV = 1.13 +/- 0.03) using recombinant chorismate...

Purification, N-terminal amino acid sequence and properties of hydroxymethylbilane synthase (porphobilinogen deaminase) from Escherichia coli.

Hart, G J, Abell, C, Battersby, A R

Hydroxymethylbilane synthase (porphobilinogen deaminase) was purified to apparent homogeneity from Escherichia coli. The enzyme is a monomer of Mr approx. 40,000. The Km for porphobilinogen and...

Investigation of putative active-site lysine residues in hydroxymethylbilane synthase. Preparation and characterization of mutants in which (a) Lys-55, (b) Lys-59 and (c) both Lys-55 and Lys-59 have been replaced by glutamine.

Hädener, A, Alefounder, P R, Hart, G J, Abell, C, Battersby, A R

A new construct carrying the hemC gene was transformed into Escherichia coli, resulting in approx. 1000-fold over-expression of hydroxymethylbilane synthase (HMBS). This construct was used to...

Studies on the mechanism of hydroxymethylbilane synthase concerning the role of arginine residues in substrate binding.

Lander, M, Pitt, A R, Alefounder, P R, Bardy, D, Abell, C, Battersby, A R

The role of conserved arginine residues in hydroxymethylbilane synthase was investigated by replacing these residues in the enzyme from Escherichia coli with leucine residues by using site-directed...

Escherichia coli L-aspartate-alpha-decarboxylase: preprotein processing and observation of reaction intermediates by electrospray mass spectrometry.

Ramjee, M K, Genschel, U, Abell, C, Smith, A G

The Escherichia coli panD gene, encoding l-aspartate-alpha-decarboxylase, was cloned by PCR, and shown to complement a panD mutant defective in beta-alanine biosynthesis. Aspartate decarboxylase is a...

The final step of pantothenate biosynthesis in higher plants: cloning and characterization of pantothenate synthetase from Lotus japonicus and Oryza sativum (rice).

Genschel, U, Powell, C A, Abell, C, Smith, A G

We have isolated a Lotus japonicus cDNA for pantothenate (vitamin B(5)) synthetase (PS) by functional complementation of an Escherichia coli panC mutant (AT1371). A rice (Oryza sativum) expressed...

Chemical force microscopy with active enzymes.

Fiorini, M, McKendry, R, Cooper, M A, Rayment, T, Abell, C

The adhesion forces have been measured between an atomic force microscope tip derivatized with an active enzyme, shikimate kinase, and an ATP mimic immobilized on a gold surface. Experiments with...

Cloning and sequencing of the Escherichia coli panB gene, which encodes ketopantoate hydroxymethyltransferase, and overexpression of the enzyme.

Jones, C E, Brook, J M, Buck, D, Abell, C, Smith, A G

The panB gene from Escherichia coli, encoding the first enzyme of the pantothenate biosynthesis pathway, ketopantoate hydroxymethyltransferase (KPHMT), has been isolated by functional complementation...

Evidence that pyridoxal phosphate modification of lysine residues (Lys-55 and Lys-59) causes inactivation of hydroxymethylbilane synthase (porphobilinogen deaminase).

Miller, A D, Packman, L C, Hart, G J, Alefounder, P R, Abell, C, Battersby, A R

A recombinant strain of Escherichia coli has been constructed that produces approx. 200 times the amount of hydroxymethylbilane synthase found in wild-type E. coli [Hart, Abell & Battersby (1986)...

Escherichia coli chorismate synthase: a deuterium kinetic-isotope effect under single-turnover and steady-state conditions shows that a flavin intermediate forms before the C-(6proR)-H bond is cleaved.

Bornemann, S, Balasubramanian, S, Coggins, J R, Abell, C, Lowe, D J, Thorneley, R N

We report the observation of a deuterium kinetic isotope effect for the conversion of 5-enolpyruvylshikimate-3-phosphate into chorismate (6proR2HV = 1.13 +/- 0.03) using recombinant chorismate...

Purification, N-terminal amino acid sequence and properties of hydroxymethylbilane synthase (porphobilinogen deaminase) from Escherichia coli.

Hart, G J, Abell, C, Battersby, A R

Hydroxymethylbilane synthase (porphobilinogen deaminase) was purified to apparent homogeneity from Escherichia coli. The enzyme is a monomer of Mr approx. 40,000. The Km for porphobilinogen and...

Investigation of putative active-site lysine residues in hydroxymethylbilane synthase. Preparation and characterization of mutants in which (a) Lys-55, (b) Lys-59 and (c) both Lys-55 and Lys-59 have been replaced by glutamine.

Hädener, A, Alefounder, P R, Hart, G J, Abell, C, Battersby, A R

A new construct carrying the hemC gene was transformed into Escherichia coli, resulting in approx. 1000-fold over-expression of hydroxymethylbilane synthase (HMBS). This construct was used to...

Studies on the mechanism of hydroxymethylbilane synthase concerning the role of arginine residues in substrate binding.

Lander, M, Pitt, A R, Alefounder, P R, Bardy, D, Abell, C, Battersby, A R

The role of conserved arginine residues in hydroxymethylbilane synthase was investigated by replacing these residues in the enzyme from Escherichia coli with leucine residues by using site-directed...

Escherichia coli L-aspartate-alpha-decarboxylase: preprotein processing and observation of reaction intermediates by electrospray mass spectrometry.

Ramjee, M K, Genschel, U, Abell, C, Smith, A G

The Escherichia coli panD gene, encoding l-aspartate-alpha-decarboxylase, was cloned by PCR, and shown to complement a panD mutant defective in beta-alanine biosynthesis. Aspartate decarboxylase is a...

The final step of pantothenate biosynthesis in higher plants: cloning and characterization of pantothenate synthetase from Lotus japonicus and Oryza sativum (rice).

Genschel, U, Powell, C A, Abell, C, Smith, A G

We have isolated a Lotus japonicus cDNA for pantothenate (vitamin B(5)) synthetase (PS) by functional complementation of an Escherichia coli panC mutant (AT1371). A rice (Oryza sativum) expressed...

Chemical force microscopy with active enzymes.

Fiorini, M, McKendry, R, Cooper, M A, Rayment, T, Abell, C

The adhesion forces have been measured between an atomic force microscope tip derivatized with an active enzyme, shikimate kinase, and an ATP mimic immobilized on a gold surface. Experiments with...