Olsen, S. L., Adachi, I., Aihara, H., Aulchenko, V., Aushev, T., ...
A distinct peak is observed in the π^±Ψ'invariant mass distribution near 4.43 GeV in B→ Kπ^±Ψ' decays. A fit using a Breit-Wigner resonance shape yields a peak mass and width of M = 4433±...
Search for \bar{B}^0 →Λ_c^+\bar{Λ}_c^- decay at Belle (2008)
Uchida, Y., Ozaki, H., Kichimi, H., Adachi, I., Aihara, H., Arinstein, K., ...
We search for the doubly charmed baryonic decay \bar{B}^0 →Λ_c^+\bar{Λ}_c^-, in a data sample of 520×106 B\bar{B} events accumulated at the Υ(4S) resonance with the Belle detector at the KEKB...
Diagnostics Development in SRRC (2008)
K. T. Hsu, C. H. Kuo, Jenny Chen, C. S. Chen, K. K. Lin, C. C. Kuo, ...
Abstract. There are several new developments in diagnostics at the SRRC. These new developments include an orbit feedback system, tune monitor, filling pattern monitor, time-domain coupled-bunch...
Observation of D_s1(2536)^+ → D^+π^-K^+ and angular decomposition of D_s1(2536)^+ →D^*+K_S^0 (2008)
Balagura, V., Adachi, I., Aihara, H., Arinstein, K., Aulchenko, V., Aushev, T., ...
Using 462 fb^{-1} of e^+e^- annihilation data recorded by the Belle detector, we report the first observation of the decay D_s1(2536)^+ → D+π^-K^+. The ratio of branching fractions...
Observation of the Ψ(4415)→ D\bar{D}_2^* (2460) Decay Using Initial-State Radiation (2008)
Pakhlova, G., Adachi, I., Aihara, H., Arinstein, K., Aulchenko, V., Aushev, T., ...
We report measurements of the exclusive cross section for e^+e^- →D^0D^-π^+ over the center-of-mass energy range 4.0 GeV to 5.0 GeV with initial-state radiation and the first observation of the...
Pakhlova, G., Adachi, I., Aihara, H., Arinstein, K., Aulchenko, V., Aushev, T., ...
We report measurements of the exclusive cross section for e^+e^ →D\bar{D}, where D=D^0 or D^+, in the center-of-mass energy range from the D\bar{D} threshold to 5 GeV with initial-state...
Observation of e^+e^- → K^+K^-J/Ψ via initial-state radiation at Belle (2008)
Yuan, C. Z., Shen, C. P., Wang, P., Wang, X. L., Adachi, I., Aihara, H., ...
The process e^+e^- →K^+K^-J/Ψ is observed for the first time via initial-state radiation. The cross section of e^+e^- → K^+K^-J/Ψ for center-of-mass energies between threshold and 6.0 GeV is...
Time-Dependent CP-Violating Asymmetry in B^0→p^0γ Decays (2008)
Ushiroda, Y., Sumisawa, K., Taniguchi, N., Adachi, I., Aihara, H., Arinstein, K., ...
We report the first measurement of CP-violation parameters in B^0 → p^0γ decays based on a data sample of 657×10^6B\bar{B} pairs collected with the Belle detector at the KEKB asymmetric-energy...
Operation Experiences and Development of the Control System in the SRRC Abstract (2007)
Jenny Chen, C. H. Kuo, K. T. Hsu, K. K. Lin, C. J. Wang, J. S. Chen, ...
The control system of the SRRC is a two-level system. Console level computers consist of workstations and PCs. The device levels consist of VME based embedded systems. PC based system are also used...
Dalseno, J., Adachi, I., Aihara, H., Aushev, T., Bakich, A. M., Balagura, V., ...
We present a measurement of the branching fraction and time-dependent CP violation parameters for B^0 → D^*+D^*-K^{0}_{S} decays. These results are obtained from a 414 fb^{-1} data sample that...
Multi-phonon Raman scattering in GaN nanowires (2007)
Dhara, S., Chandra, Sharat, Mangamma, G., Kalavathi, S., Shankar, P., Nair, K. G. M., ...
UV Raman scattering studies show longitudinal optical (LO) mode up to 4th order in wurtzite GaN nanowire system. Frohlich interaction of electron with the long range electrostatic field of ionic...
Measurements of exclusive B^{0}_{s} decays at the γ(5S) resonance (2007)
Drutskoy, A., Abe, K., Adachi, I., Aihara, H., Anipko, D., Bakich, A. M., ...
Several exclusive B^{O}_{s} decays are studied using a 1.86 fb^{-1} data sample collected at the Υ(5S) resonance with the Belle detector at the KEKB asymmetric energy e^+e^- collider. In the...
Search for B decays into η'p, η'K^*, η'φ, η'ω and η'η^(') (2007)
Schümann, J., Wang, C. H., Abe, K., Aihara, H., Anipko, D., Arinstein, K., ...
We report on a search for the exclusive two-body charmless hadronic B meson decays B → η'ρ, B → η'K^*, B^0 → ηφ, B^0 → η'ω, and B0 →η'η^('). The results are obtained from a data...
Search for Invisible Decay of the Υ(1S) (2007)
Tajima, O., Hayashii, H., Hazumi, M., Inami, K., Iwasaki, Y., Uehara, S., ...
We report results of a search for the invisible decay of the Υ(1S) via the Υ(3S)→π+π-Υ(1S) transition using a data sample of 2.9 fb^{-1} at the Υ(3S) resonance. The data were collected with...
Observation of B^+ → Λ^+_c Λ^－_c K^+ and B^0 → Λ^+_c Λ^－_c K^0 Decays (2006)
Gabyshev, N., Abe, K., Abe, K., Adachi, I., Aihara, H., Asano, Y., ...
We report the first measurements of the doubly charmed baryonic B decays B → Λ^+_c Λ^-_c K. The B+ → Λ^+_c Λ^-_c K+ decay is observed with a branching fraction of (6.5^{+1.0}_{-0.9}...
Studies of $\gamma\gamma\to\Lambda\bar{\Lambda},\Sigma^0\bar{\Sigma^0}$ production at Belle (2006)
Kuo, C. C., Collaboration, For The Belle
Cross sections for hyperon pair production from two-photon collisions, $\gamma\gamma\to\Lambda\bar{\Lambda},\Sigma^0\bar{\Sigma^0}$, are measured in the 2-4 GeV energy region at Belle, using 464...
Measurement of gamma gamma -> p p-bar production at Belle (2005)
Kuo, C. C., Collaboration, For The Belle
A high precision study of the process gamma gamma -> p p-bar has been performed using a data sample of 89/fb collected with the Belle detector at the KEKB e+e- collider. The cross section of p p-bar...
Large spin effects in Coulomb blockade of Fe/MgO/Fe tunnel junctions (2005)
Wulfhekel, W., Ernst, A., Henk, J., Bruno, P., Kirschner, J., Zavaliche, F., ...
ATS-G resin was studied using the TICA technique. Effects of air and nitrogen cure environments were accessed and the results were compared with those from a previous study on BADABBA. In general,...
Lee, C. Y-C., Kuo, C. C., Lee, N. C.
Air cure effects on the tensile and fracture behavior are studied with an acetylene terminated sulfone resin. It is found that in all instances, the air cure improves the mechanical properties. As...
Multiscale Photonic Data Fusion Networks and Their Interfaces (1998)
Sawchuk, Alexander A., Kuo, C. C., Li, Victor, Willner, Alan
We describe results for the period 30 September 1995 - 29 November 1997 in a basic research program on Multiscale Photonic Data Fusion Networks and Their Interfaces, part of the DoD Focused Research...
Chlamydia pneumoniae (TWAR) in coronary arteries of young adults (15-34 years old).
Kuo, C C, Grayston, J T, Campbell, L A, Goo, Y A, Wissler, R W, Benditt, E P
An association of Chlamydia pneumoniae with atherosclerosis of coronary and carotid arteries and aorta has been found by seroepidemiology and by demonstration of the organism in atheromata....
Malinverni, R, Kuo, C C, Campbell, L A, Lee, A, Grayston, J T
We studied the effects of two antibiotic regimens on the course of Chlamydia pneumoniae infection in the lungs of Swiss Webster mice. After intranasal challenge with isolates AR-388 (1.3 x 10(7)...
Kuo, C C, Jackson, L A, Lee, A, Grayston, J T
The in vitro susceptibilities of Chlamydia pneumoniae isolates to macrolide, tetracycline, and quinolone antibiotics were determined. Tetracycline, clarithromycin, and erythromycin had the lowest...
In vitro drug susceptibility of Chlamydia sp. strain TWAR.
The in vitro susceptibilities of eight Chlamydia sp. strain TWAR isolates were tested against tetracycline, erythromycin, penicillin, ampicillin, sulfisoxazole, and a new drug, trospectomycin. The...
Kuo, C C, Jackson, L A, Campbell, L A, Grayston, J T
Chlamydia pneumoniae (TWAR) is a recently recognized third species of the genus Chlamydia that causes acute respiratory disease. It is distinct from the other two chlamydial species that infect...
Van Voorhis, W C, Barrett, L K, Sweeney, Y T, Kuo, C C, Patton, D L
Chlamydia trachomatis-associated female infertility and ectopic pregnancy are caused by postinflammatory fibrosis and scarring of the upper genital tract. Scarring of the upper genital tract is...
Binding of the glycan of the major outer membrane protein of Chlamydia trachomatis to HeLa cells.
Recent studies have shown that the major outer membrane protein (MOMP) of Chlamydia trachomatis is glycosylated. The glycan of the MOMP of C. trachomatis serovar L2 was separated from the...
Perez Melgosa, M, Kuo, C C, Campbell, L A
Chlamydia pneumoniae is a human respiratory pathogen. Unlike the other two Chlamydia species, no species-specific antigen has been defined for C. pneumoniae. An immunoreactive clone containing a...
Effects of quinolone analog CI-960 in a monkey model of Chlamydia trachomatis salpingitis.
Patton, D L, Cosgrove, Y T, Kuo, C C, Campbell, L A
Several quinolones have been shown to have antichlamydial activity in vitro and in vivo. We evaluated the effects of the quinolone CI-960 (Parke-Davis) on primary or repeated chlamydial infection in...
Patton, D L, Wang, S K, Kuo, C C
Nonoxynol 9 (non-9) is the active ingredient in a wide variety of vaginal contraceptive preparations. The manufacturer recommendation for optimal contraceptive practice is repeated application every...
Unique ultrastructure in the elementary body of Chlamydia sp. strain TWAR.
Chi, E Y, Kuo, C C, Grayston, J T
The ultrastructure of two prototype strains (TW-183 and AR-39) of Chlamydia sp. strain TWAR was described. The TWAR elementary body (EB) demonstrated a unique morphology and structure distinct from...
In vitro susceptibility of human vascular wall cells to infection with Chlamydia pneumoniae.
Godzik, K L, O'Brien, E R, Wang, S K, Kuo, C C
Chlamydia pneumoniae is a common respiratory pathogen. Recent studies have demonstrated the presence of C. pneumoniae in coronary and aortic atherosclerotic lesions. To study the role of C....
Sequence analysis of the gene encoding the Chlamydia pneumoniae DnaK protein homolog.
Kornak, J M, Kuo, C C, Campbell, L A
The antigen-coding region of a 4.2-kb PstI fragment of Chlamydia pneumoniae (pLC3), which encodes a 75-kDa immunoreactive protein recognized during human C. pneumoniae infection, was localized to a...
Evidence that the major outer membrane protein of Chlamydia trachomatis is glycosylated.
The major outer membrane protein (MOMP) of Chlamydia trachomatis was determined to be a glycoprotein on the basis of susceptibility to glycosidase digestion and the presence of carbohydrate by...
Sequence analysis of the major outer membrane protein gene of Chlamydia pneumoniae.
Perez Melgosa, M, Kuo, C C, Campbell, L A
Compared with the major outer membrane proteins (MOMPs) of the other chlamydial species, the Chlamydia pneumoniae MOMP appears to be less antigenically complex, and as determined by immunoblot...
Structural and antigenic analysis of Chlamydia pneumoniae.
Campbell, L A, Kuo, C C, Grayston, J T
Several isolates of Chlamydia pneumoniae were compared with each other and to Chlamydia trachomatis and Chlamydia psittaci by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and...
Identification of lectin-binding proteins in Chlamydia species.
Lectin-binding proteins of chlamydiae were detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting. All three Chlamydia species tested expressed two proteins when...
Neeper, I D, Patton, D L, Kuo, C C
Time-lapse cinematography was used to study the growth cycle of Chlamydia trachomatis in primary cell cultures of human amnion. Twelve preterm and twelve term placentas were obtained within 8 h of...
Isolation and sequence analysis of the Chlamydia pneumoniae GroE operon.
Kikuta, L C, Puolakkainen, M, Kuo, C C, Campbell, L A
Chlamydia pneumoniae has emerged as an important human respiratory pathogen. From a lambda gt11 gene bank constructed from C. pneumoniae isolate AR-39 DNA, an immunoreactive plaque containing a...
Ultrastructural study of entry of Chlamydia strain TWAR into HeLa cells.
Kuo, C C, Chi, E Y, Grayston, J T
Ultrastructural studies of the entry of Chlamydia strain TWAR into HeLa cells showed that the elementary bodies first attach to host cells by the pointed end, secure other binding sites on the host...
Surface antigens of Chlamydia trachomatis were studied by immunogold staining with monoclonal antibodies and by electron microscopy. The serovar- and subspecies-specific epitopes were the most...
Yong, E C, Chi, E Y, Chen, W J, Kuo, C C
We have previously shown that human polymorphonuclear leukocytes (PMNs) killed organisms belonging to both human biovars of Chlamydia trachomatis. However, the mechanism of destruction was still...
Stephens, R S, Kuo, C C, Newport, G, Agabian, N
DNA obtained from Chlamydia trachomatis (serovar L2) was partially digested with DNase I and inserted into the beta-galactosidase gene of bacteriophage lambda gt11. Seven recombinants were selected...
Seventeen monoclonal antibodies (MAs) were tested. Five of seven serovar-specific MAs from five different serovars and two of five subspecies-specific MAs showed neutralizing activity as well as...
Chlamydia trachomatis species-specific epitope detected on mouse biovar outer membrane protein.
A common antigenic determinant on the chlamydial major outer membrane protein was detected on each of the three Chlamydia trachomatis biovars (trachoma, lymphogranuloma venereum, and mouse). This...
Immunotypes of Chlamydia trachomatis isolates in Seattle, Washington.
Kuo, C C, Wang, S P, Holmes, K K, Grayston, J T
The immunotype distribution of 493 Chlamydia trachomatis isolates from 467 adults and 26 infants in the Seattle area from 1965 to 1982 is presented. All except one of the isolates from adults were...
Detection of Chlamydia pneumoniae by polymerase chain reaction.
Campbell, L A, Perez Melgosa, M, Hamilton, D J, Kuo, C C, Grayston, J T
While criteria for serodiagnosis of Chlamydia pneumoniae infection are well established, isolation of the organism is often difficult. To increase detection of this organism, C. pneumoniae-specific...
Puolakkainen, M, Kuo, C C, Shor, A, Wang, S P, Grayston, J T, Campbell, L A
The antigen-specific serological response to Chlamydia pneumoniae was studied in 45 adults with coronary artery atherosclerosis and compared with that in 40 adults with acute respiratory infection....
Factors affecting viability and growth in HeLa 229 cells of Chlamydia sp. strain TWAR.
Two prototype isolates (TW-183 and AR-39) of Chlamydia sp. strain TWAR were used to study factors affecting growth of this organism in HeLa 229 cells. The results showed that an incubation...
Amino acid requirements for growth of two isolates of Chlamydia pneumoniae were studied and compared with those for one strain of Chlamydia trachomatis in a HeLa 229 cell culture. It was shown that...
Serological response to Chlamydia pneumoniae infection.
Campbell, L A, Kuo, C C, Wang, S P, Grayston, J T
The human serological response was analyzed by using sera from patients who were serologically positive but isolation negative for Chlamydia pneumoniae and from patients with proven C. pneumoniae...
Barnes, R C, Wang, S P, Kuo, C C, Stamm, W E
The technical complexity of determining the serovar of Chlamydia trachomatis strains has limited the use of serotyping in clinical and epidemiologic studies. We developed a simple method for rapidly...
Identification of a new group of Chlamydia psittaci strains called TWAR.
Kuo, C C, Chen, H H, Wang, S P, Grayston, J T
A new group of Chlamydia psittaci strains has been identified. They are called TWAR after the laboratory designation of the first two isolates. Twelve strains were isolated from pharyngeal swabs of...
Campbell, L A, Kuo, C C, Grayston, J T
Several molecular techniques were used for comparison of the novel Chlamydia agent, TWAR, with Chlamydia trachomatis and Chlamydia psittaci. Unlike all serotypes of C. trachomatis and most strains of...
Wang, S K, Patton, D L, Kuo, C C
Ascorbic acid (vitamin C) is an essential nutrient for humans. It may also be needed by Chlamydia trachomatis, an intracellular bacterium. We investigated the effects of vitamin C on the growth of C....
Stephens, R S, Kuo, C C, Tam, M R
Monoclonal antibodies which recognize the species-specific major outer membrane protein antigen of Chlamydia trachomatis were used for immunofluorescence staining of chlamydial inclusions in cell...
Formalinized Chlamydia trachomatis organisms as antigen in the micro-immunofluorescence test.
Wang, S P, Kuo, C C, Grayston, J T
Chlamydia trachomatis organisms grown in HeLa 229 cell cultures were purified and formalinized for use in the micro-immunofluorescence test. As test antigens, they were stable when stored unfrozen at...
Reticulate bodies as single antigen in Chlamydia trachomatis serology with microimmunofluorescence.
Yong, E C, Chinn, J S, Caldwell, H D, Kuo, C C
Formalin-fixed, purified reticulate bodies (RB) of Chlamydia trachomatis immunotype C/TW-3/OT were used as a serological test antigen in the microimmunofluorescence test. The sensitivity and...
Comparative susceptibility of eleven mammalian cell lines to infection with trachoma organisms.
Croy, T R, Kuo, C C, Wang, S P
Eleven mammalian cell lines, HeLa 229, HeLa M, Hep-2, FT, BHK-21, Vero, MK-2, MPK, L-WO5A2, McCoy, and L-929, were tested for their susceptibility to infection with Trachoma strains TW-3 (type C,...
A mouse model of Chlamydia pneumoniae strain TWAR pneumonitis.
Yang, Z P, Kuo, C C, Grayston, J T
Chlamydia pneumoniae is a common cause of acute respiratory infections in humans. We evaluated mice as experimental animals for C. pneumoniae. Intranasal inoculation of Swiss Webster mice with C....
Campbell, L A, Kuo, C C, Thissen, R W, Grayston, J T
Chlamydia sp. strain TWAR is a unique Chlamydia sp. that causes acute respiratory disease. A gene bank consisting of TWAR isolate AR-39 DNA in pUC19 was screened with anti-AR-39 rabbit immune sera....
Taylor, L D, Carmack, C E, Schramm, S R, Mashayekh, R, Higgins, K M, Kuo, C C, ...
We have generated transgenic mice that express a diverse repertoire of human sequence immunoglobulins. The expression of this repertoire is directed by light and heavy chain minilocus transgenes...
Toxic effect of human polymorphonuclear leukocytes on Chlamydia trachomatis.
Yong, E C, Klebanoff, S J, Kuo, C C
The effect of human polymorphonuclear leukocytes (PMNs) on Chlamydia trachomatis was studied. Both trachoma (B/TW-5/OT) and lymphogranuloma venereum (L2/434/Bu) biotypes were rapidly inactivated by...
Reactivation of Chlamydia trachomatis lung infection in mice by cortisone.
Yang, Y S, Kuo, C C, Chen, W J
To study the latency, chronicity, and recurrent nature of chlamydial infection, we attempted to reactivate Chlamydia trachomatis lung infection in mice by immunosuppressive therapy with cortisone....
Brunham, R C, Kuo, C C, Cles, L, Holmes, K K
We studied 95 women with uncomplicated Chlamydia trachomatis cervical infection. Quantitative isolation of C. trachomatis was performed in HeLa 229 cells, and the results were correlated with serum...
Brunham, R C, Martin, D H, Kuo, C C, Wang, S P, Stevens, C E, Hubbard, T, ...
A lymphocyte transformation (LT) assay for the study of the cellular immune response to Chlamydia trachomatis in humans is described. Subjects studied included 9 newborns whose mothers were C....
Stephens, R S, Chen, W J, Kuo, C C
Suppression of the inflammatory reaction with daily doses of cortisone acetate or cyclophosphamide substantially prolonged the pulmonary infection in mice which had been intranasally inoculated with...
Brunham, R C, Martin, D H, Hubbard, T W, Kuo, C C, Critchlow, C W, Cles, L D, ...
Lymphocyte transformation (LT) responses to Chlamydia trachomatis, to four other microbial antigens, and to phytohemagglutinin (PHA) were studied in 201 women during pregnancy and/or 3-18 wk...
B cell development in mice that lack one or both immunoglobulin kappa light chain genes.
Chen, J, Trounstine, M, Kurahara, C, Young, F, Kuo, C C, Xu, Y, ...
We have generated mice that lack the ability to produce immunoglobulin (Ig) kappa light chains by targeted deletion of J kappa and C kappa gene segments and the intervening sequences in mouse...
Comparative infectivity of trachoma organisms in HeLa 229 cells and egg cultures.
Kuo, C C, Wang, S P, Grayston, J T
Thirty-two trachoma strains were simultaneously titrated in the yolk sac of embryonated chicken eggs and in HeLa 229 cells pretreated with diethylaminoethyl-dextran to determine the relative...
Interaction of Chlamydia trachomatis organisms and HeLa 229 cells.
The infection of HeLa 229 cells in monolayer culture with trachoma (B/TW-5/OT) and lymphogranuloma venereum (LGV) (L2/434/Bu) organism was studied in terms of two parameters: radioactivity counts of...
Immediate cytotoxicity of Chlamydia trachomatis for mouse peritoneal macrophages.
The toxicity of Chlamydia trachomatis was studied with mouse peritoneal macrophage culture. Inoculation of 30 inclusion-forming units of trachoma B/TW-5/OT organisms and 250 inclusion-forming units...
Rapidly inactivating K+ current (KA current) is recorded from rat hippocampal neurons by whole-cell patch-clamp technique and suitable voltage protocols. It is found that imipramine, a commonly...
1. Both inward and outward unitary Li+ currents through the L-type Ca2+ channel and discrete block of such currents by either internal or external Ca2+ are recorded. Detailed kinetic analyses are...
1. Two major types of ion-ion interaction are demonstrated in the pore of the L-type Ca2+ channel, the 'lock-in' and the 'enhancement' effect. The former denotes that the ion at a certain site in the...
Block of the L-type Ca2+ channel pore by external and internal Mg2+ in rat phaeochromocytoma cells.
1. Three to eight micromolar external Mg2+ produces discrete block of the unitary inward currents through the L-type Ca2+ channel carried by 300 mM external Li+. Like the Ca2+ block, increasing Li+...
Chlamydia pneumoniae (TWAR) in coronary arteries of young adults (15-34 years old).
Kuo, C C, Grayston, J T, Campbell, L A, Goo, Y A, Wissler, R W, Benditt, E P
An association of Chlamydia pneumoniae with atherosclerosis of coronary and carotid arteries and aorta has been found by seroepidemiology and by demonstration of the organism in atheromata....
Malinverni, R, Kuo, C C, Campbell, L A, Lee, A, Grayston, J T
We studied the effects of two antibiotic regimens on the course of Chlamydia pneumoniae infection in the lungs of Swiss Webster mice. After intranasal challenge with isolates AR-388 (1.3 x 10(7)...
Kuo, C C, Jackson, L A, Lee, A, Grayston, J T
The in vitro susceptibilities of Chlamydia pneumoniae isolates to macrolide, tetracycline, and quinolone antibiotics were determined. Tetracycline, clarithromycin, and erythromycin had the lowest...
In vitro drug susceptibility of Chlamydia sp. strain TWAR.
The in vitro susceptibilities of eight Chlamydia sp. strain TWAR isolates were tested against tetracycline, erythromycin, penicillin, ampicillin, sulfisoxazole, and a new drug, trospectomycin. The...
Kuo, C C, Jackson, L A, Campbell, L A, Grayston, J T
Chlamydia pneumoniae (TWAR) is a recently recognized third species of the genus Chlamydia that causes acute respiratory disease. It is distinct from the other two chlamydial species that infect...
Van Voorhis, W C, Barrett, L K, Sweeney, Y T, Kuo, C C, Patton, D L
Chlamydia trachomatis-associated female infertility and ectopic pregnancy are caused by postinflammatory fibrosis and scarring of the upper genital tract. Scarring of the upper genital tract is...
Binding of the glycan of the major outer membrane protein of Chlamydia trachomatis to HeLa cells.
Recent studies have shown that the major outer membrane protein (MOMP) of Chlamydia trachomatis is glycosylated. The glycan of the MOMP of C. trachomatis serovar L2 was separated from the...
Perez Melgosa, M, Kuo, C C, Campbell, L A
Chlamydia pneumoniae is a human respiratory pathogen. Unlike the other two Chlamydia species, no species-specific antigen has been defined for C. pneumoniae. An immunoreactive clone containing a...
Effects of quinolone analog CI-960 in a monkey model of Chlamydia trachomatis salpingitis.
Patton, D L, Cosgrove, Y T, Kuo, C C, Campbell, L A
Several quinolones have been shown to have antichlamydial activity in vitro and in vivo. We evaluated the effects of the quinolone CI-960 (Parke-Davis) on primary or repeated chlamydial infection in...
Patton, D L, Wang, S K, Kuo, C C
Nonoxynol 9 (non-9) is the active ingredient in a wide variety of vaginal contraceptive preparations. The manufacturer recommendation for optimal contraceptive practice is repeated application every...
Unique ultrastructure in the elementary body of Chlamydia sp. strain TWAR.
Chi, E Y, Kuo, C C, Grayston, J T
The ultrastructure of two prototype strains (TW-183 and AR-39) of Chlamydia sp. strain TWAR was described. The TWAR elementary body (EB) demonstrated a unique morphology and structure distinct from...
In vitro susceptibility of human vascular wall cells to infection with Chlamydia pneumoniae.
Godzik, K L, O'Brien, E R, Wang, S K, Kuo, C C
Chlamydia pneumoniae is a common respiratory pathogen. Recent studies have demonstrated the presence of C. pneumoniae in coronary and aortic atherosclerotic lesions. To study the role of C....
Sequence analysis of the gene encoding the Chlamydia pneumoniae DnaK protein homolog.
Kornak, J M, Kuo, C C, Campbell, L A
The antigen-coding region of a 4.2-kb PstI fragment of Chlamydia pneumoniae (pLC3), which encodes a 75-kDa immunoreactive protein recognized during human C. pneumoniae infection, was localized to a...
Evidence that the major outer membrane protein of Chlamydia trachomatis is glycosylated.
The major outer membrane protein (MOMP) of Chlamydia trachomatis was determined to be a glycoprotein on the basis of susceptibility to glycosidase digestion and the presence of carbohydrate by...
Sequence analysis of the major outer membrane protein gene of Chlamydia pneumoniae.
Perez Melgosa, M, Kuo, C C, Campbell, L A
Compared with the major outer membrane proteins (MOMPs) of the other chlamydial species, the Chlamydia pneumoniae MOMP appears to be less antigenically complex, and as determined by immunoblot...
Structural and antigenic analysis of Chlamydia pneumoniae.
Campbell, L A, Kuo, C C, Grayston, J T
Several isolates of Chlamydia pneumoniae were compared with each other and to Chlamydia trachomatis and Chlamydia psittaci by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and...
Identification of lectin-binding proteins in Chlamydia species.
Lectin-binding proteins of chlamydiae were detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting. All three Chlamydia species tested expressed two proteins when...
Neeper, I D, Patton, D L, Kuo, C C
Time-lapse cinematography was used to study the growth cycle of Chlamydia trachomatis in primary cell cultures of human amnion. Twelve preterm and twelve term placentas were obtained within 8 h of...
Isolation and sequence analysis of the Chlamydia pneumoniae GroE operon.
Kikuta, L C, Puolakkainen, M, Kuo, C C, Campbell, L A
Chlamydia pneumoniae has emerged as an important human respiratory pathogen. From a lambda gt11 gene bank constructed from C. pneumoniae isolate AR-39 DNA, an immunoreactive plaque containing a...
Ultrastructural study of entry of Chlamydia strain TWAR into HeLa cells.
Kuo, C C, Chi, E Y, Grayston, J T
Ultrastructural studies of the entry of Chlamydia strain TWAR into HeLa cells showed that the elementary bodies first attach to host cells by the pointed end, secure other binding sites on the host...
Surface antigens of Chlamydia trachomatis were studied by immunogold staining with monoclonal antibodies and by electron microscopy. The serovar- and subspecies-specific epitopes were the most...
Yong, E C, Chi, E Y, Chen, W J, Kuo, C C
We have previously shown that human polymorphonuclear leukocytes (PMNs) killed organisms belonging to both human biovars of Chlamydia trachomatis. However, the mechanism of destruction was still...
Stephens, R S, Kuo, C C, Newport, G, Agabian, N
DNA obtained from Chlamydia trachomatis (serovar L2) was partially digested with DNase I and inserted into the beta-galactosidase gene of bacteriophage lambda gt11. Seven recombinants were selected...
Seventeen monoclonal antibodies (MAs) were tested. Five of seven serovar-specific MAs from five different serovars and two of five subspecies-specific MAs showed neutralizing activity as well as...
Chlamydia trachomatis species-specific epitope detected on mouse biovar outer membrane protein.
A common antigenic determinant on the chlamydial major outer membrane protein was detected on each of the three Chlamydia trachomatis biovars (trachoma, lymphogranuloma venereum, and mouse). This...
Immunotypes of Chlamydia trachomatis isolates in Seattle, Washington.
Kuo, C C, Wang, S P, Holmes, K K, Grayston, J T
The immunotype distribution of 493 Chlamydia trachomatis isolates from 467 adults and 26 infants in the Seattle area from 1965 to 1982 is presented. All except one of the isolates from adults were...
Detection of Chlamydia pneumoniae by polymerase chain reaction.
Campbell, L A, Perez Melgosa, M, Hamilton, D J, Kuo, C C, Grayston, J T
While criteria for serodiagnosis of Chlamydia pneumoniae infection are well established, isolation of the organism is often difficult. To increase detection of this organism, C. pneumoniae-specific...
Puolakkainen, M, Kuo, C C, Shor, A, Wang, S P, Grayston, J T, Campbell, L A
The antigen-specific serological response to Chlamydia pneumoniae was studied in 45 adults with coronary artery atherosclerosis and compared with that in 40 adults with acute respiratory infection....
Factors affecting viability and growth in HeLa 229 cells of Chlamydia sp. strain TWAR.
Two prototype isolates (TW-183 and AR-39) of Chlamydia sp. strain TWAR were used to study factors affecting growth of this organism in HeLa 229 cells. The results showed that an incubation...
Amino acid requirements for growth of two isolates of Chlamydia pneumoniae were studied and compared with those for one strain of Chlamydia trachomatis in a HeLa 229 cell culture. It was shown that...
Serological response to Chlamydia pneumoniae infection.
Campbell, L A, Kuo, C C, Wang, S P, Grayston, J T
The human serological response was analyzed by using sera from patients who were serologically positive but isolation negative for Chlamydia pneumoniae and from patients with proven C. pneumoniae...
Barnes, R C, Wang, S P, Kuo, C C, Stamm, W E
The technical complexity of determining the serovar of Chlamydia trachomatis strains has limited the use of serotyping in clinical and epidemiologic studies. We developed a simple method for rapidly...
Identification of a new group of Chlamydia psittaci strains called TWAR.
Kuo, C C, Chen, H H, Wang, S P, Grayston, J T
A new group of Chlamydia psittaci strains has been identified. They are called TWAR after the laboratory designation of the first two isolates. Twelve strains were isolated from pharyngeal swabs of...
Campbell, L A, Kuo, C C, Grayston, J T
Several molecular techniques were used for comparison of the novel Chlamydia agent, TWAR, with Chlamydia trachomatis and Chlamydia psittaci. Unlike all serotypes of C. trachomatis and most strains of...
Wang, S K, Patton, D L, Kuo, C C
Ascorbic acid (vitamin C) is an essential nutrient for humans. It may also be needed by Chlamydia trachomatis, an intracellular bacterium. We investigated the effects of vitamin C on the growth of C....
Stephens, R S, Kuo, C C, Tam, M R
Monoclonal antibodies which recognize the species-specific major outer membrane protein antigen of Chlamydia trachomatis were used for immunofluorescence staining of chlamydial inclusions in cell...
Formalinized Chlamydia trachomatis organisms as antigen in the micro-immunofluorescence test.
Wang, S P, Kuo, C C, Grayston, J T
Chlamydia trachomatis organisms grown in HeLa 229 cell cultures were purified and formalinized for use in the micro-immunofluorescence test. As test antigens, they were stable when stored unfrozen at...
Reticulate bodies as single antigen in Chlamydia trachomatis serology with microimmunofluorescence.
Yong, E C, Chinn, J S, Caldwell, H D, Kuo, C C
Formalin-fixed, purified reticulate bodies (RB) of Chlamydia trachomatis immunotype C/TW-3/OT were used as a serological test antigen in the microimmunofluorescence test. The sensitivity and...
Comparative susceptibility of eleven mammalian cell lines to infection with trachoma organisms.
Croy, T R, Kuo, C C, Wang, S P
Eleven mammalian cell lines, HeLa 229, HeLa M, Hep-2, FT, BHK-21, Vero, MK-2, MPK, L-WO5A2, McCoy, and L-929, were tested for their susceptibility to infection with Trachoma strains TW-3 (type C,...
A mouse model of Chlamydia pneumoniae strain TWAR pneumonitis.
Yang, Z P, Kuo, C C, Grayston, J T
Chlamydia pneumoniae is a common cause of acute respiratory infections in humans. We evaluated mice as experimental animals for C. pneumoniae. Intranasal inoculation of Swiss Webster mice with C....
Campbell, L A, Kuo, C C, Thissen, R W, Grayston, J T
Chlamydia sp. strain TWAR is a unique Chlamydia sp. that causes acute respiratory disease. A gene bank consisting of TWAR isolate AR-39 DNA in pUC19 was screened with anti-AR-39 rabbit immune sera....
Taylor, L D, Carmack, C E, Schramm, S R, Mashayekh, R, Higgins, K M, Kuo, C C, ...
We have generated transgenic mice that express a diverse repertoire of human sequence immunoglobulins. The expression of this repertoire is directed by light and heavy chain minilocus transgenes...
Toxic effect of human polymorphonuclear leukocytes on Chlamydia trachomatis.
Yong, E C, Klebanoff, S J, Kuo, C C
The effect of human polymorphonuclear leukocytes (PMNs) on Chlamydia trachomatis was studied. Both trachoma (B/TW-5/OT) and lymphogranuloma venereum (L2/434/Bu) biotypes were rapidly inactivated by...
Reactivation of Chlamydia trachomatis lung infection in mice by cortisone.
Yang, Y S, Kuo, C C, Chen, W J
To study the latency, chronicity, and recurrent nature of chlamydial infection, we attempted to reactivate Chlamydia trachomatis lung infection in mice by immunosuppressive therapy with cortisone....
Brunham, R C, Kuo, C C, Cles, L, Holmes, K K
We studied 95 women with uncomplicated Chlamydia trachomatis cervical infection. Quantitative isolation of C. trachomatis was performed in HeLa 229 cells, and the results were correlated with serum...
Brunham, R C, Martin, D H, Kuo, C C, Wang, S P, Stevens, C E, Hubbard, T, ...
A lymphocyte transformation (LT) assay for the study of the cellular immune response to Chlamydia trachomatis in humans is described. Subjects studied included 9 newborns whose mothers were C....
Stephens, R S, Chen, W J, Kuo, C C
Suppression of the inflammatory reaction with daily doses of cortisone acetate or cyclophosphamide substantially prolonged the pulmonary infection in mice which had been intranasally inoculated with...
Brunham, R C, Martin, D H, Hubbard, T W, Kuo, C C, Critchlow, C W, Cles, L D, ...
Lymphocyte transformation (LT) responses to Chlamydia trachomatis, to four other microbial antigens, and to phytohemagglutinin (PHA) were studied in 201 women during pregnancy and/or 3-18 wk...
B cell development in mice that lack one or both immunoglobulin kappa light chain genes.
Chen, J, Trounstine, M, Kurahara, C, Young, F, Kuo, C C, Xu, Y, ...
We have generated mice that lack the ability to produce immunoglobulin (Ig) kappa light chains by targeted deletion of J kappa and C kappa gene segments and the intervening sequences in mouse...
Comparative infectivity of trachoma organisms in HeLa 229 cells and egg cultures.
Kuo, C C, Wang, S P, Grayston, J T
Thirty-two trachoma strains were simultaneously titrated in the yolk sac of embryonated chicken eggs and in HeLa 229 cells pretreated with diethylaminoethyl-dextran to determine the relative...
Interaction of Chlamydia trachomatis organisms and HeLa 229 cells.
The infection of HeLa 229 cells in monolayer culture with trachoma (B/TW-5/OT) and lymphogranuloma venereum (LGV) (L2/434/Bu) organism was studied in terms of two parameters: radioactivity counts of...
Immediate cytotoxicity of Chlamydia trachomatis for mouse peritoneal macrophages.
The toxicity of Chlamydia trachomatis was studied with mouse peritoneal macrophage culture. Inoculation of 30 inclusion-forming units of trachoma B/TW-5/OT organisms and 250 inclusion-forming units...
1. Both inward and outward unitary Li+ currents through the L-type Ca2+ channel and discrete block of such currents by either internal or external Ca2+ are recorded. Detailed kinetic analyses are...
1. Two major types of ion-ion interaction are demonstrated in the pore of the L-type Ca2+ channel, the 'lock-in' and the 'enhancement' effect. The former denotes that the ion at a certain site in the...
Block of the L-type Ca2+ channel pore by external and internal Mg2+ in rat phaeochromocytoma cells.
1. Three to eight micromolar external Mg2+ produces discrete block of the unitary inward currents through the L-type Ca2+ channel carried by 300 mM external Li+. Like the Ca2+ block, increasing Li+...
Rapidly inactivating K+ current (KA current) is recorded from rat hippocampal neurons by whole-cell patch-clamp technique and suitable voltage protocols. It is found that imipramine, a commonly...
Jackson, L. A., Campbell, L. A., Schmidt, R. A., Kuo, C. C., Cappuccio, A. L., Lee, M. J., ...
Chlamydia pneumoniae has been detected in atherosclerotic plaque, raising the question of whether this detection is specific to atheromatous tissue. To evaluate this question, we tested...
Chou, W. Y., Chang, H. P., Huang, C. H., Kuo, C. C., Tong, L., Chang, G. G.
Pigeon liver malic enzyme was inactivated and cleaved at Asp141, Asp194, and Asp464 by the Cu2+-ascorbate system in acidic environment. Site-specific mutagenesis was performed at these putative...
NOVEL SUPERSTRUCTURES OF ALKALI-DOPED C60 FILMS ON Cu(111) AND Ag(111) SURFACES
C. C. KUO, C. H. LIN, WOEI WU PAI, K. C. LIN, T. B. TANG
We report a scanning tunneling microscopy (STM) study of potassium (K)-doped C60 (KxC60) on Cu(111) and Ag(111), with focus on the observation of novel K-induced superstructures. On Cu(111),...
Beam Stability At Srrc Storage Ring
Weng Chang Chen, H. P. Chang, J. R. Chen, Y. Cheng, K. T. Hsu, ...
To satisfy the need of users of the synchrotron radiation at SRRC, the photon flux at the end station has to be kept at a constant with minimum fluctuation. The sources of flux variation attributable...
Performance of the SRRC Storage Ring and Wiggler Commissioning
C. C. Kuo, K. T. Hsu, G. H. Luo, W.K. Lau, Ch. Wang, L.H. Chang, ...
A 1.3 GeV synchrotron radiation storage ring at SRRC has been operated for more than a year since October 1993. Starting from April 1994, the machine has been open to the user community. In February...
The Observation Of The Longitudinal Coupled Bunch Motion On Streak Camera At Srrc
Wang Hsu Lau, M. H. Wang, K. T. Hsu, W. K. Lau, C. S. Hsue, H. J. Tsai, ...
A Hamamatsu C5680 streak camera was set up to observe the beam motion at one of the synchrotron light ports at SRRC. The single bunch, two bunches and a few bunches longitudinal beam motion were...
Study Of Longitudinal Coupled-Bunch Instabilities In The Srrc Storage Ring
Lau Wang Hsu, W. K. Lau, M. H. Wang, K. T. Hsu, L. H. Chang, Ch. Wang, ...
INTRODUCTION In a storage ring, longitudinal coupled-bunch instabilities of an electron beam are driven by the interaction of the beam with its environment, especially with the high Q components in...
Damping Rates Of The Srrc Storage Ring
Hsu Kuo Lau, K. T. Hsu, C. C. Kuo, W. K. Lau, W. T. Weng
The SRRC storage ring is a low emittance synchrotron radiation machine with nominal operation energy 1.3 GeV. The design damping time due to synchrotron radiation is 10.7, 14.4, 8.7 ms for the...
Chlamydia pneumoniae and cardiovascular disease.
Campbell, L. A., Kuo, C. C., Grayston, J. T.
Chlamydia pneumoniae is a ubiquitous pathogen that causes acute respiratory disease. The spectrum of C. pneumoniae infection has been extended to atherosclerosis and its clinical manifestations....