Maathuis, A., Deutz, N.E.P., Dijkema, C., ...
This study introduces a stable-isotope metabolic approach employing [U-(13)C]glucose that, as a novelty, allows selective profiling of the human intestinal microbial metabolic products of...
Vecherskaya, M., Dijkema, C., Ramirez Saad, H, Stams, A.J.M.
An obligate methanotrophic bacterium, strain MTS, was isolated from a methane-fed microaerobic denitrifying bioreactor. 16S rRNA and DNA–DNA hybridization analysis revealed that this organism was...
Phase behavior of phosphatidylglycerol in spinach thylakoid membranes as revealed by 31P-NMR (2008)
Krumova, S.K.B., Dijkema, C., Garab, G.
Non-bilayer lipids account for about half of the total lipid content in chloroplast thylakoid membranes. This lends high propensity of the thylakoid lipid mixture to participate in different phases...
Zelle, R.M., Dijkema, C., Winkler, A.A., ...
Malic acid is a potential biomass-derivable "building block" for chemical synthesis. Since wild-type Saccharomyces cerevisiae strains produce only low levels of malate, metabolic engineering is...
Danova-Alt, R., Dijkema, C., Köck, M.
Phosphite (Phi, H(2)PO(3)(-)), being the active part of several fungicides, has been shown to influence not only the fungal metabolism but also the development of phosphate-deficient plants. However,...
Mars, A.E., Budde, M.A.W., Lai, M.H., Dijkema, C., ...
NMR analysis of 13C-labelling patterns showed that the Embden¿Meyerhof (EM) pathway is the main route for glycolysis in the extreme thermophile Caldicellulosiruptor saccharolyticus. Glucose...
Henstra, A.M., Dijkema, C., Stams, A.J.M.
The genome sequence of Archaeoglobus fulgidus VC16 encodes three CO dehydrogenase genes. Here we explore the capacity of A.¿fulgidus to use CO as growth substrate. Archaeoglobus fulgidus VC16 was...
Egert, M.G.G., Maathuis, A., Plugge, C.M., Smidt, H., ...
16S rRNA-based stable isotope probing (SIP) and nuclear magnetic resonance (NMR) spectroscopy-based metabolic profiling were used to identify bacteria fermenting glucose under conditions simulating...
Polymorphic phase behaviour of phosphatidylglycerine in spinach thylakoid membranes (2005)
Krumova, S.K.B., Dijkema, C., Garab, G.
Our data show that the phospholipids of chloroplast thylakoid membranes participate in non-lamellar phases and polymorphic changes. Although 31P NMR is sensitive solely to phospholipids, it seems...
Krumova, S.K.B., Dijkema, C., Garab, G.
In thylakoid membranes non-bilayer lipids account for about half of the lipid content. This determines the high propensity of the whole lipid mixture to participate in different lipid phases. The...
Krumova, S.K.B., Dijkema, C., Garab, G.
In thylakoid membranes non-bilayer lipids account for about half of the lipid content. This determines the high propensity of the whole lipid mixture to participate in different lipid phases. The...
Pathways of methanol conversion in a thermophilic anaerobic (55 degrees C) sludge consortium (2003)
Paulo, P.L., Stams, A.J.M., Field, J.A., Dijkema, C., Lettinga, G.
The pathway of methanol conversion by a thermophilic anaerobic consortium was elucidated by recording the fate of carbon in the presence and absence of bicarbonate and specific inhibitors. Results...
Verhees, C.H., Koot, D.G., Ettema, T.J., Dijkema, C.
The hyperthermophilic archaeon Pyrococcus furiosus possesses a modified Embden-Meyerhof pathway, including an unusual ADP-dependent glucokinase (ADP-GLK) and an ADP-dependent phosphofructokinase. In...
Intracellular pH homeostasis in the filamentous fungus Aspergillys niger (2002)
Hesse, S.J.A., Ruijter, G.J.G., Dijkema, C., Visser, J.
Intracellular pH homeostasis in the filamentous fungus Aspergillus niger was measured in real time by 31P NMR during perfusion in the NMR tube of fungal biomass immobilized in Ca2 -alginate beads....
Genevini, P., Adani, F., Veeken, A.H.M., Nierop, K.G.J., Scaglia, B., Dijkema, C.
Humic acid-like (HA-like) and core-humic acid-like (core-HA-like) were characterized during the high-rate composting process by CP-MAS 13C NMR, pyrolysis-gas chromatography (GC)/mass spectrometry...
energy yield of respiration on chloroaromatic compounds in Desulfitobacterium dehalogenans (2001)
Jansen, S., Dijkema, C., Schraa, G., Stams, A.J.M.
The amount of energy that can be conserved via halorespiration by Desulfitobacterium dehalogenans JW/IU-DC1 was determined by comparison of the growth yields of cells grown with...
Intracellular PHB conversion in a type II methanotroph studied by 13 C NMR (2001)
Vecherskaya, M., Dijkema, C., Stams, A.J.M.
Poly-g-hydroxybutyrate (PHB) formation under aerobic conditions via incorporation of [13C-2]acetate as a cosubstrate and its intracellular degradation under anaerobic conditions in a Type II...
Costa, C., Vecherskaya, M., Dijkema, C., Stams, A.J.M.
13C NMR was used to study the effect of oxygen on methanol oxidation by a type II methanotrophic bacterium isolated from a bioreactor in which methane was used as electron donor for denitrification....
Stams, A.J.M., Dijkema, C., Boone, D.R.
The pathway of propionate conversion in a syntrophic coculture of Smithella propionica and Methanospirillum hungatei JF1 was investigated by 13C-NMR spectroscopy. Cocultures produced acetate and...
High resolution [31P] nuclear magnetic resonance (NMR) spectroscopy was used to investigate the changes in phosphate metabolism and intracellular pH in intact root segments of relatively osmotic...
Denitrification with methane as electron donor in oxygen-limited bioreactors (2000)
Costa, C., Dijkema, C., Friedrich, M., Garcia-Encina, P., Fernandez-Polanco, F., Stams, A.J.M.
Bioavailability of HOCs related to soil organic matter composition (2000)
Cuypers, M.P., Grotenhuis, J.T.C., Nierop, K., Maneiro, E., Dijkema, C., Rulkens, W.H.
Krook, J., Vreugdenhil, D., Dijkema, C.
After a lag phase of 2 days, batch-grown cells of carrot (Daucus carota L.) cv. Flakkese entered the exponential growth phase and started to accumulate sucrose and hexoses. Short-term feeding...
Dijkema, C., Schols, H.A., Voragen, A.G.J.
Lactococcus lactis subsp. cremoris B891 grown on whey permeate produced an exopolysaccharide containing -Gal and -Glc in a molar ratio of 2:3. The polysaccharide was partially O-acetylated. By means...
Transglycosidase activity of Bifidobacterium adolescentis DSM 20083 alpha-galactosidase (1999)
Hartemink, R., Beldman, G., Pitson, S., Dijkema, C., Schols, H.A., ...
Isolation and characterisation of novel lactulose-based oligosaccharides (1999)
Schols, H.A., Lucas-Lokhorst, G., Dijkema, C., Ernste, S., Voragen, A.G.J.
Dijkema, C., Schols, H.A., Beldman, G., Voragen, A.G.J.
The chemical structure of an exopolysaccharide produced by Lactococcus lactis subsp. cremoris B40 is studied, explaining earlier reported analytical discrepancies. The EPS was found to have a...
Isolation and characterisation of novel lactulose-based oligosaccharides. (1997)
Schols, H.A., Lucas-Lokhorst, G., Dijkema, C., Ernste, S., Voragen, A.G.J.
D-Xylose catabolism in Bacteroides xylanolyticus. (1994)
Biesterveld, S., Kok, M.D., Dijkema, C., Zehnder, A.J.B., Stams, A.J.M.
Kengen, S.W.M., Dijkema, C., Stams, A.J.M.
The main pathway for the fermentation of maltose or cellobiose by the hyperthermophile Pyrococcus furiosus was investigated by in vivo NMR and by enzyme measurements. Addition of [1-13C]glucose to...
Advances in molecular genetics and biochemistry of carbohydrate metabolism. (1990)
Visser, J., Witteveen, C.F.B., Dijkema, C., Hondmann, D.H.A., ...
L-arabinose and D-xylose catabolism in Aspergillus niger. (1989)
Witteveen, C.F.B., Busink, R., Dijkema, C., Swart, K., Visser, J.
Pentose metabolism in Aspergillus niger. (1988)
Witteveen, C.F.B., Busink, R., Dijkema, C., Swart, K., Visser, J.
D-xylose and L-arabinose metabolism in Aspergillus niger. (1988)
Witteveen, C.F.B., Busink, R., Dijkema, C., Swart, K., Visser, J.
13C-NMR study of propionate degradation by a methanogenic coculture. (1987)
Houwen, F.P., Dijkema, C., Schoenmakers, C.H.M., Stams, A.J.M., Zehnder, A.J.B.
Optical spectroscopy and magnetic resonance of photosynthetic particles and model systems. (1986)
Schaafsma, T.J., Searle, G.F.W., Dijkema, C., Nedbal, L., Gast, P., ...
Genetic and physiological studies on carbon metabolism in Aspergillus nidulans (1983)
Bos, C.J., Dijkema, C., Kester, H.C.M., Kobus, G., Visser, J.
Stams, A. J. M., Dijkema, C., Boone, D. R.
The pathway of propionate conversion in a syntrophic coculture of Smithella propionica and Methanospirillum hungatei JF1 was investigated by 13C-NMR spectroscopy. Cocultures produced acetate and...
13C NMR studies of carbon metabolism in the hyphal fungus Aspergillus nidulans.
Dijkema, C, Kester, H C, Visser, J
Natural-abundance high-resolution 13C NMR spectra (linewidth, 10 Hz) of the hyphal fungus Aspergillus nidulans have been obtained after growth on glycolytic or gluconeogenic carbon sources. Various...
Stams, A. J. M., Dijkema, C., Boone, D. R.
The pathway of propionate conversion in a syntrophic coculture of Smithella propionica and Methanospirillum hungatei JF1 was investigated by 13C-NMR spectroscopy. Cocultures produced acetate and...
13C NMR studies of carbon metabolism in the hyphal fungus Aspergillus nidulans.
Dijkema, C, Kester, H C, Visser, J
Natural-abundance high-resolution 13C NMR spectra (linewidth, 10 Hz) of the hyphal fungus Aspergillus nidulans have been obtained after growth on glycolytic or gluconeogenic carbon sources. Various...