Schizosaccharomyces pombe glycosylation mutant with altered cell surface properties.
Ballou, C E, Ballou, L, Ball, G
Mutagenesis of Schizosaccharomyces pombe cells yielded a strain that made reduced amounts of invertase. A comparison of the O- and N-linked carbohydrate chains of the wild-type and mutant...
Häusler, A, Ballou, L, Ballou, C E, Robbins, P W
The Saccharomyces cerevisiae MNT1 gene encodes a Golgi mannosyltransferase. Gene disruption of the MNT1 locus leads to a greater than 90% reduction of specific alpha-1,2-mannosyltransferase activity...
Vanadate-resistant yeast mutants are defective in protein glycosylation.
Ballou, L, Hitzeman, R A, Lewis, M S, Ballou, C E
Spontaneous recessive orthovanadate-resistant mutants of Saccharomyces cerevisiae were obtained in five complementation groups, and all show defects in protein glycosylation that mimic the previously...
Revision of the oligosaccharide structures of yeast carboxypeptidase Y.
Ballou, L, Hernandez, L M, Alvarado, E, Ballou, C E
The N-linked oligosaccharides from baker's yeast carboxypeptidase Y were analyzed by 1H NMR and specific mannosidase digestion and found to be identical to those from the Saccharomyces cerevisiae...
Methanol production by Mycobacterium smegmatis.
Mycobacterium smegmatis cells produce [3H]methanol when incubated with [methyl-3H]methionine. The methanol is derived from S-adenosylmethionine rather than methyltetrahydrofolate. M. smegmatis cells...
Tunicamycin inhibition of epispore formation in Saccharomyces cerevisiae.
The ascopore wall of Saccharomyces cerevisiae was found to contain more protein, polymeric glucosamine, and beta-glucan than the vegetative cell wall, which was enriched in mannoprotein relative to...
Amylooligosaccharides from Mycobacterium smegmatis.
In early stationary phase of growth, Mycobacterium smegmatis cultures accumulate amylooligosaccharides (alpha 1 leads to 4-glucooligosaccharides) up to the undecasaccharide. Although M. smegmatis...
Morphogenic effects of alpha-factor on Saccharomyces cerevisiae a cells.
Lipke, P N, Taylor, A, Ballou, C E
Saccharomyces cerevisiae mating type a cells enlarged and elongated when exposed to alpha-factor, a sex pheromone produced by mating-type alpha cells. This morphogensis required exogenous-D-glucose,...
Mutants that require exogenous D-glucosamine for growth were isolated from Saccharomyces cerevisiae X2180-1A after ethyl methane sulfonate mutagenesis. Class A auxotrophs fail to grow on yeast...
Cell-cell recognition in yeast: isolation of intact alpha-agglutinin from Saccharomyces kluyveri.
The heat-labile sexual agglutinin from Saccharomyces kluyveri strain 17 (alpha-agglutinin) has been isolated in an apparently intact form from wild-type and mnn1 (glycosylation-defective) cells. The...
Chiral synthesis of D- and L-myo-inositol 1,4,5-trisphosphate.
Chiral inositols (D-chiro-inositol from D-pinitol and L-chiro-inositol from L-quebrachitol) were converted to the 3,4-di-O-benzyl ethers, which were selectively benzoylated to yield the...
Antibodies were raised against Saccharomyces cerevisiae a-cells that had been exposed to the sex pheromone, alpha-factor. After adsorption of the antiserum with diploid cells, antibodies remained...
Polymethylpolysaccharide synthesis in an ethionine-resistant mutant of Mycobacterium smegmatis.
Mutants of Mycobacterium smegmatis were selected for resistance to ethionine in an effort to obtain methylation-defective strains that were altered in their ability to make methylmannose...
The yeast Saccharomyces cerevisiae X2180 strain with the mnn1 mnn2 mnn9 mutations, all of which affect mannoprotein glycosylation, synthesizes N-linked oligosaccharides having the following...
Carbohydrate chains on yeast carboxypeptidase Y are phosphorylated.
Hashimoto, C, Cohen, R E, Zhang, W J, Ballou, C E
Carboxypeptidase Y, a vacuolar enzyme from Saccharomyces cerevisiae, was digested with endo-beta-N-acetyl-D-glucosaminidase H to release the four oligosaccharide chains that are linked to asparagine...
Ballou, L, Gopal, P, Krummel, B, Tammi, M, Ballou, C E
A mutant of Saccharomyces cerevisiae with the genotype mnn1 mnn2 mnn9 gls1 synthesizes mannoproteins with oligosaccharides having the composition Glc3Man10Glc-NAc2- owing to the mnn9 defect, which...
Tsai, P K, Dell, A, Ballou, C E
Fast-atom-bombardment mass spectrometry has been applied to acetylated neutral and phosphorylated oligosaccharides from yeast glycoproteins and to their acetolysis products. Although acetylation...
Saccharomyces cerevisiae invertase (EC 3.2.1.26) isolated from wild-type strain X2180 can be resolved by isoelectric focusing into at least seven bands revealed by an activity stain. Most of this...
Temperature-sensitive glucosamine auxotroph of Saccharomyces cerevisiae.
Ballou, L, Grove, J R, Roon, R J, Wiggs, J, Ballou, C E
Temperature-sensitive revertants were isolated from Saccharomyces cerevisiae D-glucosamine auxotrophs previously obtained in this laboratory (W. L. Whelan and C. E. Ballou, J. Bacteriol....
Isolation of glucose-containing high-mannose glycoprotein core oligosaccharides.
Tsai, P K, Ballou, L, Esmon, B, Schekman, R, Ballou, C E
The total cell wall mannoprotein has been isolated from a mutant of Saccharomyces cerevisiae that fails to remove the glucose units of the dolichol-linked precursor after transfer of the...
Yeast manno-protein biosynthesis: solubilization and selective assay of four mannosyltransferases.
Using appropriate yeast strains and exogenous acceptors, we have devised specific assays for four mannosyltransferase activities involved in biosynthesis of the carbohydrate outer chain of yeast...
Schizosaccharomyces pombe glycosylation mutant with altered cell surface properties.
Ballou, C E, Ballou, L, Ball, G
Mutagenesis of Schizosaccharomyces pombe cells yielded a strain that made reduced amounts of invertase. A comparison of the O- and N-linked carbohydrate chains of the wild-type and mutant...
Häusler, A, Ballou, L, Ballou, C E, Robbins, P W
The Saccharomyces cerevisiae MNT1 gene encodes a Golgi mannosyltransferase. Gene disruption of the MNT1 locus leads to a greater than 90% reduction of specific alpha-1,2-mannosyltransferase activity...
Vanadate-resistant yeast mutants are defective in protein glycosylation.
Ballou, L, Hitzeman, R A, Lewis, M S, Ballou, C E
Spontaneous recessive orthovanadate-resistant mutants of Saccharomyces cerevisiae were obtained in five complementation groups, and all show defects in protein glycosylation that mimic the previously...
Revision of the oligosaccharide structures of yeast carboxypeptidase Y.
Ballou, L, Hernandez, L M, Alvarado, E, Ballou, C E
The N-linked oligosaccharides from baker's yeast carboxypeptidase Y were analyzed by 1H NMR and specific mannosidase digestion and found to be identical to those from the Saccharomyces cerevisiae...
Methanol production by Mycobacterium smegmatis.
Mycobacterium smegmatis cells produce [3H]methanol when incubated with [methyl-3H]methionine. The methanol is derived from S-adenosylmethionine rather than methyltetrahydrofolate. M. smegmatis cells...
Tunicamycin inhibition of epispore formation in Saccharomyces cerevisiae.
The ascopore wall of Saccharomyces cerevisiae was found to contain more protein, polymeric glucosamine, and beta-glucan than the vegetative cell wall, which was enriched in mannoprotein relative to...
Amylooligosaccharides from Mycobacterium smegmatis.
In early stationary phase of growth, Mycobacterium smegmatis cultures accumulate amylooligosaccharides (alpha 1 leads to 4-glucooligosaccharides) up to the undecasaccharide. Although M. smegmatis...
Morphogenic effects of alpha-factor on Saccharomyces cerevisiae a cells.
Lipke, P N, Taylor, A, Ballou, C E
Saccharomyces cerevisiae mating type a cells enlarged and elongated when exposed to alpha-factor, a sex pheromone produced by mating-type alpha cells. This morphogensis required exogenous-D-glucose,...
Mutants that require exogenous D-glucosamine for growth were isolated from Saccharomyces cerevisiae X2180-1A after ethyl methane sulfonate mutagenesis. Class A auxotrophs fail to grow on yeast...
Cell-cell recognition in yeast: isolation of intact alpha-agglutinin from Saccharomyces kluyveri.
The heat-labile sexual agglutinin from Saccharomyces kluyveri strain 17 (alpha-agglutinin) has been isolated in an apparently intact form from wild-type and mnn1 (glycosylation-defective) cells. The...
Chiral synthesis of D- and L-myo-inositol 1,4,5-trisphosphate.
Chiral inositols (D-chiro-inositol from D-pinitol and L-chiro-inositol from L-quebrachitol) were converted to the 3,4-di-O-benzyl ethers, which were selectively benzoylated to yield the...
Antibodies were raised against Saccharomyces cerevisiae a-cells that had been exposed to the sex pheromone, alpha-factor. After adsorption of the antiserum with diploid cells, antibodies remained...
Polymethylpolysaccharide synthesis in an ethionine-resistant mutant of Mycobacterium smegmatis.
Mutants of Mycobacterium smegmatis were selected for resistance to ethionine in an effort to obtain methylation-defective strains that were altered in their ability to make methylmannose...
The yeast Saccharomyces cerevisiae X2180 strain with the mnn1 mnn2 mnn9 mutations, all of which affect mannoprotein glycosylation, synthesizes N-linked oligosaccharides having the following...
Carbohydrate chains on yeast carboxypeptidase Y are phosphorylated.
Hashimoto, C, Cohen, R E, Zhang, W J, Ballou, C E
Carboxypeptidase Y, a vacuolar enzyme from Saccharomyces cerevisiae, was digested with endo-beta-N-acetyl-D-glucosaminidase H to release the four oligosaccharide chains that are linked to asparagine...
Ballou, L, Gopal, P, Krummel, B, Tammi, M, Ballou, C E
A mutant of Saccharomyces cerevisiae with the genotype mnn1 mnn2 mnn9 gls1 synthesizes mannoproteins with oligosaccharides having the composition Glc3Man10Glc-NAc2- owing to the mnn9 defect, which...
Tsai, P K, Dell, A, Ballou, C E
Fast-atom-bombardment mass spectrometry has been applied to acetylated neutral and phosphorylated oligosaccharides from yeast glycoproteins and to their acetolysis products. Although acetylation...
Saccharomyces cerevisiae invertase (EC 3.2.1.26) isolated from wild-type strain X2180 can be resolved by isoelectric focusing into at least seven bands revealed by an activity stain. Most of this...
Temperature-sensitive glucosamine auxotroph of Saccharomyces cerevisiae.
Ballou, L, Grove, J R, Roon, R J, Wiggs, J, Ballou, C E
Temperature-sensitive revertants were isolated from Saccharomyces cerevisiae D-glucosamine auxotrophs previously obtained in this laboratory (W. L. Whelan and C. E. Ballou, J. Bacteriol....
Isolation of glucose-containing high-mannose glycoprotein core oligosaccharides.
Tsai, P K, Ballou, L, Esmon, B, Schekman, R, Ballou, C E
The total cell wall mannoprotein has been isolated from a mutant of Saccharomyces cerevisiae that fails to remove the glucose units of the dolichol-linked precursor after transfer of the...
Yeast manno-protein biosynthesis: solubilization and selective assay of four mannosyltransferases.
Using appropriate yeast strains and exogenous acceptors, we have devised specific assays for four mannosyltransferase activities involved in biosynthesis of the carbohydrate outer chain of yeast...