C. E. Rogler

Human DNA topoisomerase I-mediated cleavage and recombination of duck hepatitis B virus DNA in vitro.

Pourquier, P, Jensen, A D, Gong, S S, Pommier, Y, Rogler, C E

In this study, we report that eukaryotic topoisomerase I (top1) can linearize the open circular DNA of duck hepatitis B virus (DHBV). Using synthetic oligonucleotides mimicking the three-strand flap...

Duck hepatitis B virus integrations in LMH chicken hepatoma cells: identification and characterization of new episomally derived integrations.

Gong, S S, Jensen, A D, Wang, H, Rogler, C E

While the cytoplasmic phase of the hepadnavirus replication cycle is well understood, very little is known about the nuclear phase. In contrast to retroviruses, proviral integration is not required...

Loss and acquisition of duck hepatitis B virus integrations in lineages of LMH-D2 chicken hepatoma cells.

Gong, S S, Jensen, A D, Rogler, C E

Hepatocellular carcinoma is the culmination of a series of genetic events which progressively alter the phenotype of a hepatocyte toward malignancy. Hepadnaviral DNA integrations are agents of...

Woodchuck hepatitis virus X protein is present in chronically infected woodchuck liver and woodchuck hepatocellular carcinomas which are permissive for viral replication.

Dandri, M, Schirmacher, P, Rogler, C E

The woodchuck hepatitis virus (WHV) X gene (WHx) is required for infectivity of WHV in woodchucks, and the gene encodes a broadly acting transcription factor. Several lines of evidence from cell...

Insulin-like growth factor II blocks apoptosis of N-myc2-expressing woodchuck liver epithelial cells.

Yang, D, Faris, R, Hixson, D, Affigne, S, Rogler, C E

N-myc2 and insulin-like growth factor II (IGF-II) are coordinately overexpressed in the great majority of altered hepatic foci, which are the earliest precancerous lesions observed in the liver of...

The hepatitis B virus (HBV) precore protein inhibits HBV replication in transgenic mice.

Guidotti, L G, Matzke, B, Pasquinelli, C, Shoenberger, J M, Rogler, C E, Chisari, F V

In this study, we examined the ability of the hepatitis B virus (HBV) precore, envelope, and X gene products to modulate HBV replication in the livers of transgenic mice that replicate the virus....

Increase in the frequency of hepadnavirus DNA integrations by oxidative DNA damage and inhibition of DNA repair.

Petersen, J, Dandri, M, Bürkle, A, Zhang, L, Rogler, C E

Persistent hepadnavirus infection leads to oxidative stress and DNA damage through increased production of toxic oxygen radicals. In addition, hepadnaviral DNA integrations into chromosomal DNA can...

Hepatitis B virus nucleocapsid particles do not cross the hepatocyte nuclear membrane in transgenic mice.

Guidotti, L G, Martinez, V, Loh, Y T, Rogler, C E, Chisari, F V

Transgenic mice that express the hepatitis B virus core protein were used to examine factors that influence the intracellular localization of nucleocapsid particles in the primary hepatocyte in vivo....

Topoisomerase I-mediated integration of hepadnavirus DNA in vitro.

Wang, H P, Rogler, C E

Hepadnaviruses integrate in cellular DNA via an illegitimate recombination mechanism, and clonally propagated integrations are present in most hepatocellular carcinomas which arise in hepadnavirus...

Features of two hepatitis B virus (HBV) DNA integrations suggest mechanisms of HBV integration.

Hino, O, Ohtake, K, Rogler, C E

Two integrated hepatitis B virus (HBV) DNA molecules were cloned from two primary hepatocellular carcinomas each containing only a single integration. One integration (C3) contained a single linear...

Insulinlike growth factor II expression and oval cell proliferation associated with hepatocarcinogenesis in woodchuck hepatitis virus carriers.

Fu, X X, Su, C Y, Lee, Y, Hintz, R, Biempica, L, Snyder, R, ...

Insulinlike growth factor II (IGF-II) is a highly mitogenic fetal growth factor suspected of regulating the growth of a wide spectrum of tissues via an autocrine or paracrine mode of action or both....

Cloning and structural analysis of integrated woodchuck hepatitis virus sequences from a chronically infected liver.

Rogler, C E, Summers, J

We have isolated and determined the structure of a recombinant clone in lambda phage Charon 30 which contains woodchuck hepatitis virus sequences integrated in woodchuck genomic DNA sequences. This...

Molecular analysis of the c-myc locus in normal tissue and in avian leukosis virus-induced lymphomas.

Neel, B G, Gasic, G P, Rogler, C E, Skalka, A M, Ju, G, Hishinuma, F, ...

We isolated molecular clones of the provirus-host cell junctions (tumor junction fragments) from two avian leukosis virus-induced lymphomas and compared the structures of these clones with a clone of...

Novel forms of woodchuck hepatitis virus DNA isolated from chronically infected woodchuck liver nuclei.

Rogler, C E, Summers, J

We cloned several unique forms of woodchuck hepatitis virus, a DNA virus closely related to hepatitis B virus, from a chronically infected woodchuck liver. Each of the three clones contained more...

Hepatitis B virus integration site in hepatocellular carcinoma at chromosome 17;18 translocation.

Hino, O, Shows, T B, Rogler, C E

Integrated hepatitis B virus (HBV) DNA is almost invariably found in hepatocellular carcinomas (HCC) which develop in HBV carriers. Integrated HBV DNAs from two single-integration HCCs (C3 and C4)...

Human DNA topoisomerase I-mediated cleavage and recombination of duck hepatitis B virus DNA in vitro.

Pourquier, P, Jensen, A D, Gong, S S, Pommier, Y, Rogler, C E

In this study, we report that eukaryotic topoisomerase I (top1) can linearize the open circular DNA of duck hepatitis B virus (DHBV). Using synthetic oligonucleotides mimicking the three-strand flap...

Duck hepatitis B virus integrations in LMH chicken hepatoma cells: identification and characterization of new episomally derived integrations.

Gong, S S, Jensen, A D, Wang, H, Rogler, C E

While the cytoplasmic phase of the hepadnavirus replication cycle is well understood, very little is known about the nuclear phase. In contrast to retroviruses, proviral integration is not required...

Loss and acquisition of duck hepatitis B virus integrations in lineages of LMH-D2 chicken hepatoma cells.

Gong, S S, Jensen, A D, Rogler, C E

Hepatocellular carcinoma is the culmination of a series of genetic events which progressively alter the phenotype of a hepatocyte toward malignancy. Hepadnaviral DNA integrations are agents of...

Woodchuck hepatitis virus X protein is present in chronically infected woodchuck liver and woodchuck hepatocellular carcinomas which are permissive for viral replication.

Dandri, M, Schirmacher, P, Rogler, C E

The woodchuck hepatitis virus (WHV) X gene (WHx) is required for infectivity of WHV in woodchucks, and the gene encodes a broadly acting transcription factor. Several lines of evidence from cell...

Insulin-like growth factor II blocks apoptosis of N-myc2-expressing woodchuck liver epithelial cells.

Yang, D, Faris, R, Hixson, D, Affigne, S, Rogler, C E

N-myc2 and insulin-like growth factor II (IGF-II) are coordinately overexpressed in the great majority of altered hepatic foci, which are the earliest precancerous lesions observed in the liver of...

The hepatitis B virus (HBV) precore protein inhibits HBV replication in transgenic mice.

Guidotti, L G, Matzke, B, Pasquinelli, C, Shoenberger, J M, Rogler, C E, Chisari, F V

In this study, we examined the ability of the hepatitis B virus (HBV) precore, envelope, and X gene products to modulate HBV replication in the livers of transgenic mice that replicate the virus....

Increase in the frequency of hepadnavirus DNA integrations by oxidative DNA damage and inhibition of DNA repair.

Petersen, J, Dandri, M, Bürkle, A, Zhang, L, Rogler, C E

Persistent hepadnavirus infection leads to oxidative stress and DNA damage through increased production of toxic oxygen radicals. In addition, hepadnaviral DNA integrations into chromosomal DNA can...

Hepatitis B virus nucleocapsid particles do not cross the hepatocyte nuclear membrane in transgenic mice.

Guidotti, L G, Martinez, V, Loh, Y T, Rogler, C E, Chisari, F V

Transgenic mice that express the hepatitis B virus core protein were used to examine factors that influence the intracellular localization of nucleocapsid particles in the primary hepatocyte in vivo....

Topoisomerase I-mediated integration of hepadnavirus DNA in vitro.

Wang, H P, Rogler, C E

Hepadnaviruses integrate in cellular DNA via an illegitimate recombination mechanism, and clonally propagated integrations are present in most hepatocellular carcinomas which arise in hepadnavirus...

Features of two hepatitis B virus (HBV) DNA integrations suggest mechanisms of HBV integration.

Hino, O, Ohtake, K, Rogler, C E

Two integrated hepatitis B virus (HBV) DNA molecules were cloned from two primary hepatocellular carcinomas each containing only a single integration. One integration (C3) contained a single linear...

Insulinlike growth factor II expression and oval cell proliferation associated with hepatocarcinogenesis in woodchuck hepatitis virus carriers.

Fu, X X, Su, C Y, Lee, Y, Hintz, R, Biempica, L, Snyder, R, ...

Insulinlike growth factor II (IGF-II) is a highly mitogenic fetal growth factor suspected of regulating the growth of a wide spectrum of tissues via an autocrine or paracrine mode of action or both....

Cloning and structural analysis of integrated woodchuck hepatitis virus sequences from a chronically infected liver.

Rogler, C E, Summers, J

We have isolated and determined the structure of a recombinant clone in lambda phage Charon 30 which contains woodchuck hepatitis virus sequences integrated in woodchuck genomic DNA sequences. This...

Molecular analysis of the c-myc locus in normal tissue and in avian leukosis virus-induced lymphomas.

Neel, B G, Gasic, G P, Rogler, C E, Skalka, A M, Ju, G, Hishinuma, F, ...

We isolated molecular clones of the provirus-host cell junctions (tumor junction fragments) from two avian leukosis virus-induced lymphomas and compared the structures of these clones with a clone of...

Novel forms of woodchuck hepatitis virus DNA isolated from chronically infected woodchuck liver nuclei.

Rogler, C E, Summers, J

We cloned several unique forms of woodchuck hepatitis virus, a DNA virus closely related to hepatitis B virus, from a chronically infected woodchuck liver. Each of the three clones contained more...

Hepatitis B virus integration site in hepatocellular carcinoma at chromosome 17;18 translocation.

Hino, O, Shows, T B, Rogler, C E

Integrated hepatitis B virus (HBV) DNA is almost invariably found in hepatocellular carcinomas (HCC) which develop in HBV carriers. Integrated HBV DNAs from two single-integration HCCs (C3 and C4)...

Selective amplification of periportal transitional cells precedes formation of hepatocellular carcinoma in SV40 large tag transgenic mice.

Schirmacher, P., Held, W. A., Yang, D., Biempica, L., Rogler, C. E.

In a major urinary protein (MUP)-promoter/simian virus 40 (SV40)Tag transgenic mouse line (MT-D2) the liver-directed, androgen-regulated transgene expression leads to synchronized pathology resulting...