C. F. Thurston

Publication List Details

Period

1994 - 1994

Number

16

Co-Authors

The cel4 Gene of Agaricus bisporus Encodes a β-Mannanase

Tang, C. M., Waterman, L. D., Smith, M. H., Thurston, C. F.

Mannases have industrial uses in food and pulp industries, and their regulation may influence development of the mushrooms of commercially important basidiomycetes. We expressed an Agaricus bisporus...

The cel3 gene of Agaricus bisporus codes for a modular cellulase and is transcriptionally regulated by the carbon source.

Chow, C M, Yagüe, E, Raguz, S, Wood, D A, Thurston, C F

A 52-kDa protein, CEL3, has been separated from the culture filtrate of Agaricus bisporus during growth on cellulose. A PCR-derived probe was made, with a degenerate oligodeoxynucleotide derived from...

Evidence for messenger ribonucleic acid of an ammonium-inducible glutamate dehydrogenase and synthesis, covalent modification, and degradation of enzyme subunits in uninduced Chlorella sorokiniana cells.

Turner, K J, Bascomb, N F, Lynch, J J, Molin, W T, Thurston, C F, Schmidt, R R

The cells of Chlorella sorokiniana cultured in nitrate medium contain no detectable catalytic activity of an ammonium-inducible nicotinamide adenine dinucleotide phosphate-specific glutamate...

Control of isocitrate lyase synthesis in Chlorella fusca var. vacuolata. The basal activity of the enzyme and the kinetics of induction.

Thurston, C F

1. Isocitrate lyase activity was measured in non-induced Chlorella fusca var. vacuolata cells. 2. During exponential autotrophic growth about 1-2 molecules of the enzyme per cell were present. 3. In...

The kinetic of degradation of chondroitin of sulphates and hyaluronic acid by chondroitinase form Proteus vulgaris.

Thurston, C F

Km and Vmax. were determined for the degradation by chondroitinase of chondroitin 4-sulphate, 4-sulphate-proteoglycna, chondroitin 6-sulphate, dermatan sulphate and hyaluronic acid. Degradation of...

Disappearance of isocitrate lyase enzyme from cells of Chlorella pyrenoidosa

John, P. C. L., Thurston, C. F., Syrett, P. J.

1. When acetate-adapted cells of Chlorella are suspended in nitrogen-free medium and supplied with glucose, isocitrate lyase activity disappears from the cells at a rate of about 9%/h. This loss of...

Control of isocitrate lyase synthesis in Chlorella fusca var. vacuolata. Rate of enzyme synthesis in the presence and absence of acetate measured by [35S]methionine labelling and immunoprecipitation.

Dunham, S M, Thurston, C F

The rate of increase of isocitrate lyase activity was measured in darkened Chlorella fusca var. vaculoata cultures in the presence and absence of acetate and compared with the rate of incorporation...

The cel4 Gene of Agaricus bisporus Encodes a β-Mannanase

Tang, C. M., Waterman, L. D., Smith, M. H., Thurston, C. F.

Mannases have industrial uses in food and pulp industries, and their regulation may influence development of the mushrooms of commercially important basidiomycetes. We expressed an Agaricus bisporus...

The cel3 gene of Agaricus bisporus codes for a modular cellulase and is transcriptionally regulated by the carbon source.

Chow, C M, Yagüe, E, Raguz, S, Wood, D A, Thurston, C F

A 52-kDa protein, CEL3, has been separated from the culture filtrate of Agaricus bisporus during growth on cellulose. A PCR-derived probe was made, with a degenerate oligodeoxynucleotide derived from...

Evidence for messenger ribonucleic acid of an ammonium-inducible glutamate dehydrogenase and synthesis, covalent modification, and degradation of enzyme subunits in uninduced Chlorella sorokiniana cells.

Turner, K J, Bascomb, N F, Lynch, J J, Molin, W T, Thurston, C F, Schmidt, R R

The cells of Chlorella sorokiniana cultured in nitrate medium contain no detectable catalytic activity of an ammonium-inducible nicotinamide adenine dinucleotide phosphate-specific glutamate...

Control of isocitrate lyase synthesis in Chlorella fusca var. vacuolata. The basal activity of the enzyme and the kinetics of induction.

Thurston, C F

1. Isocitrate lyase activity was measured in non-induced Chlorella fusca var. vacuolata cells. 2. During exponential autotrophic growth about 1-2 molecules of the enzyme per cell were present. 3. In...

The kinetic of degradation of chondroitin of sulphates and hyaluronic acid by chondroitinase form Proteus vulgaris.

Thurston, C F

Km and Vmax. were determined for the degradation by chondroitinase of chondroitin 4-sulphate, 4-sulphate-proteoglycna, chondroitin 6-sulphate, dermatan sulphate and hyaluronic acid. Degradation of...

Disappearance of isocitrate lyase enzyme from cells of Chlorella pyrenoidosa

John, P. C. L., Thurston, C. F., Syrett, P. J.

1. When acetate-adapted cells of Chlorella are suspended in nitrogen-free medium and supplied with glucose, isocitrate lyase activity disappears from the cells at a rate of about 9%/h. This loss of...

Control of isocitrate lyase synthesis in Chlorella fusca var. vacuolata. Rate of enzyme synthesis in the presence and absence of acetate measured by [35S]methionine labelling and immunoprecipitation.

Dunham, S M, Thurston, C F

The rate of increase of isocitrate lyase activity was measured in darkened Chlorella fusca var. vaculoata cultures in the presence and absence of acetate and compared with the rate of incorporation...