Kappler, U., Friedrich, C. G., Truper, H. G., Dahl, C.
The pathway of thiosulfate oxidation in the facultatively chemolithotrophic, sulfur-oxidizing bacterium Starkeya novella (formerly Thiobacillus novellus) has not been established beyond doubt....
Kappler, U., Friedrich, C. G., Truper, H. G., Dahl, C.
The pathway of thiosulfate oxidation in the facultatively chemolithotrophic, sulfur-oxidizing bacterium Starkeya novella (formerly Thiobacillus novellus) has not been established beyond doubt....
Wodara, C, Bardischewsky, F, Friedrich, C G
A 13-kb genomic region of Paracoccus dentrificans GB17 is involved in lithotrophic thiosulfate oxidation. Adjacent to the previously reported soxB gene (C. Wodara, S. Kostka, M. Egert, D. P. Kelly,...
Wodara, C, Kostka, S, Egert, M, Kelly, D P, Friedrich, C G
The coding region for lithotrophic sulfur oxidation (Sox) in Paracoccus denitrificans GB17 was identified by isolation of a transposon Tn5-mob mutant with a Sox- phenotype (strain TP19). The...
Transfer and expression of degradative and antibiotic resistance plasmids in acidophilic bacteria.
The genetic accessibility of selected acidophilic bacteria was investigated to evaluate their applicability to degrading pollutants in acidic environments. The IncP1 antibiotic resistance plasmids...
Mittenhuber, G, Sonomoto, K, Egert, M, Friedrich, C G
For the identification of the DNA region responsible for the sulfur-oxidizing ability (Sox) of Thiosphaera pantotropha, we used previously isolated Tn5-mob insertional Sox- mutants. For seven...
Oelmüller, U, Schlegel, H G, Friedrich, C G
The functional half-lives of Alcaligenes eutrophus hydrogenase mRNAs were determined by physiological studies. Evidence was obtained for a functional half-life of about 1 h for the soluble NAD-linked...
Kärst, U, Suetin, S, Friedrich, C G
In Alcaligenes eutrophus, the formation of the hydrogenases and of five new peptides is subject to the hydrogenase control system. Of these, the B peptide was purified to homogeneity. This protein...
Tn5-induced mutations affecting sulfur-oxidizing ability (Sox) of Thiosphaera pantotropha.
Mutants of Thiosphaera pantotropha defective in chemolithoautotrophic growth were obtained by transpositional mutagenesis with Tn5 coding for kanamycin resistance. The suicide vehicle for introducing...
Expression of hydrogenase in Alcaligenes spp. is altered by interspecific plasmid exchange.
Friedrich, B, Friedrich, C G, Meyer, M, Schlegel, H G
Alcaligenes hydrogenophilus was found to contain a soluble and a particulate hydrogenase whose control and structure differed in part from that in Alcaligenes eutrophus. One of at least two plasmids...
Alcaligenes eutrophus did not form the key enzymes of autotrophic metabolism, the soluble and particulate hydrogenases and ribulosebisphosphate carboxylase (RuBPC), during heterotrophic growth on...
Nickel requirement for active hydrogenase formation in Alcaligenes eutrophus.
Friedrich, B, Heine, E, Finck, A, Friedrich, C G
The nickel-dependent chemolithoautotrophic growth of Alcaligenes eutrophus is apparently due to a requirement of nickel for active hydrogenase formation. Cells grown heterotrophically with fructose...
Alcaligenes eutrophus grew well autotrophically with molecular hydrogen at 30 degrees C, but failed to grow at 37 degrees C (Hox Ts). At this temperature the strain grew well heterotrophically with a...
Alcaligenes eutrophus formed ribulosebisphosphate carboxylase (RuBPCase; EC 4.1.1.39) when grown on fructose. Addition of sodium fluoride (NaF) to fructose minimal medium resulted in a slightly...
Nickel in the catalytically active hydrogenase of Alcaligenes eutrophus.
Friedrich, C G, Schneider, K, Friedrich, B
Nickel is a constituent of soluble and particulate hydrogenase of Alcaligenes eutrophus. Incorporation of 63Ni2+ revealed that almost the total nickel taken up by the cells was bound to the protein....
Friedrich, B, Friedrich, C G, Magasanik, B
Klebsiella aerogenes formed two N2-acetylornithine 5-aminotransferases (ACOAT) which were separable by diethylaminoethyl-cellulose chromatography. One ACOAT was repressed when the cells grew on...
Friedrich, B, Friedrich, C G, Schlegel, H G
Chorismate mutase and prephenate dehydratase from Alcaligenes autophus H16 were purified 470-fold with a yield of 24%. During the course of purification, including chromatography on diethylaminoethyl...
Friedrich, C G, Friedrich, B, Schlegel, H G
Highly purified enzymes from Alcaligenes eutrophus H 16 were used for kinetic studies. Chorismate mutase was feedback inhibited by phenylalanine. In the absence of the inhibitor, the...
Effects of lysine analogs on Penicillium chrysogenum.
Compounds structurally related to lysine were tested against Penicillium chrysogenum Wis. 54-1255 for inhibition of growth, sporulation, and penicillin formation. This strain is relatively resistant...
Wodara, C, Bardischewsky, F, Friedrich, C G
A 13-kb genomic region of Paracoccus dentrificans GB17 is involved in lithotrophic thiosulfate oxidation. Adjacent to the previously reported soxB gene (C. Wodara, S. Kostka, M. Egert, D. P. Kelly,...
Wodara, C, Kostka, S, Egert, M, Kelly, D P, Friedrich, C G
The coding region for lithotrophic sulfur oxidation (Sox) in Paracoccus denitrificans GB17 was identified by isolation of a transposon Tn5-mob mutant with a Sox- phenotype (strain TP19). The...
Transfer and expression of degradative and antibiotic resistance plasmids in acidophilic bacteria.
The genetic accessibility of selected acidophilic bacteria was investigated to evaluate their applicability to degrading pollutants in acidic environments. The IncP1 antibiotic resistance plasmids...
Mittenhuber, G, Sonomoto, K, Egert, M, Friedrich, C G
For the identification of the DNA region responsible for the sulfur-oxidizing ability (Sox) of Thiosphaera pantotropha, we used previously isolated Tn5-mob insertional Sox- mutants. For seven...
Oelmüller, U, Schlegel, H G, Friedrich, C G
The functional half-lives of Alcaligenes eutrophus hydrogenase mRNAs were determined by physiological studies. Evidence was obtained for a functional half-life of about 1 h for the soluble NAD-linked...
Kärst, U, Suetin, S, Friedrich, C G
In Alcaligenes eutrophus, the formation of the hydrogenases and of five new peptides is subject to the hydrogenase control system. Of these, the B peptide was purified to homogeneity. This protein...
Tn5-induced mutations affecting sulfur-oxidizing ability (Sox) of Thiosphaera pantotropha.
Mutants of Thiosphaera pantotropha defective in chemolithoautotrophic growth were obtained by transpositional mutagenesis with Tn5 coding for kanamycin resistance. The suicide vehicle for introducing...
Expression of hydrogenase in Alcaligenes spp. is altered by interspecific plasmid exchange.
Friedrich, B, Friedrich, C G, Meyer, M, Schlegel, H G
Alcaligenes hydrogenophilus was found to contain a soluble and a particulate hydrogenase whose control and structure differed in part from that in Alcaligenes eutrophus. One of at least two plasmids...
Alcaligenes eutrophus did not form the key enzymes of autotrophic metabolism, the soluble and particulate hydrogenases and ribulosebisphosphate carboxylase (RuBPC), during heterotrophic growth on...
Nickel requirement for active hydrogenase formation in Alcaligenes eutrophus.
Friedrich, B, Heine, E, Finck, A, Friedrich, C G
The nickel-dependent chemolithoautotrophic growth of Alcaligenes eutrophus is apparently due to a requirement of nickel for active hydrogenase formation. Cells grown heterotrophically with fructose...
Alcaligenes eutrophus grew well autotrophically with molecular hydrogen at 30 degrees C, but failed to grow at 37 degrees C (Hox Ts). At this temperature the strain grew well heterotrophically with a...
Alcaligenes eutrophus formed ribulosebisphosphate carboxylase (RuBPCase; EC 4.1.1.39) when grown on fructose. Addition of sodium fluoride (NaF) to fructose minimal medium resulted in a slightly...
Nickel in the catalytically active hydrogenase of Alcaligenes eutrophus.
Friedrich, C G, Schneider, K, Friedrich, B
Nickel is a constituent of soluble and particulate hydrogenase of Alcaligenes eutrophus. Incorporation of 63Ni2+ revealed that almost the total nickel taken up by the cells was bound to the protein....
Friedrich, B, Friedrich, C G, Magasanik, B
Klebsiella aerogenes formed two N2-acetylornithine 5-aminotransferases (ACOAT) which were separable by diethylaminoethyl-cellulose chromatography. One ACOAT was repressed when the cells grew on...
Friedrich, B, Friedrich, C G, Schlegel, H G
Chorismate mutase and prephenate dehydratase from Alcaligenes autophus H16 were purified 470-fold with a yield of 24%. During the course of purification, including chromatography on diethylaminoethyl...
Friedrich, C G, Friedrich, B, Schlegel, H G
Highly purified enzymes from Alcaligenes eutrophus H 16 were used for kinetic studies. Chorismate mutase was feedback inhibited by phenylalanine. In the absence of the inhibitor, the...
Effects of lysine analogs on Penicillium chrysogenum.
Compounds structurally related to lysine were tested against Penicillium chrysogenum Wis. 54-1255 for inhibition of growth, sporulation, and penicillin formation. This strain is relatively resistant...