Identification of an RNA binding site for human thymidylate synthase.
Chu, E, Voeller, D, Koeller, D M, Drake, J C, Takimoto, C H, Maley, G F, ...
Previous studies from this laboratory have shown that human TS mRNA translation is regulated by its protein product in a negative autoregulatory manner. In this paper, we identify an RNA binding site...
Autoregulation of human thymidylate synthase messenger RNA translation by thymidylate synthase.
Chu, E, Koeller, D M, Casey, J L, Drake, J C, Chabner, B A, Elwood, P C, ...
Thymidylate synthase (TS; 5,10-methylenetetrahydrofolate:dUMP C-methyltransferase, EC 2.1.1.45) is essential for the de novo synthesis of thymidylate, a precursor of DNA. Previous studies have shown...
Identification of in vivo target RNA sequences bound by thymidylate synthase.
Chu, E, Cogliati, T, Copur, S M, Borre, A, Voeller, D M, Allegra, C J, ...
We developed an immunoprecipitation-RNA-random PCR (rPCR) method to isolate cellular RNA sequences that bind to the folate-dependent enzyme thymidylate synthase (TS). Using this approach, nine...
Bioavailability of oral trimetrexate in patients with acquired immunodeficiency syndrome.
Rogers, P, Allegra, C J, Murphy, R F, Drake, J C, Masur, H, Poplack, D G, ...
The combination of the lipophilic antifolate trimetrexate and the rescue agent leucovorin has shown promise in the treatment of Pneumocystis carinii pneumonia in patients with acquired...
Potent antipneumocystis and antitoxoplasma activities of piritrexim, a lipid-soluble antifolate.
Kovacs, J A, Allegra, C J, Swan, J C, Drake, J C, Parrillo, J E, Chabner, B A, ...
Piritrexim, a lipid-soluble antifolate, was evaluated for its activity against Pneumocystis carinii and Toxoplasma gondii. The concentration of piritrexim needed to inhibit 50% of the catalytic...
Mechanism of pyrimethamine resistance in recent isolates of Plasmodium falciparum.
McCutchan, T F, Welsh, J A, Dame, J B, Quakyi, I A, Graves, P M, Drake, J C, ...
Clones of Plasmodium falciparum prepared from recent isolates of infected blood were studied to determine the molecular mechanism of naturally occurring pyrimethamine resistance. Total DNA, as well...
Kovacs, J A, Powell, F, Voeller, D, Allegra, C J
Isoprinosine has been reported to decrease progression to AIDS, primarily by preventing Pneumocystis carinii pneumonia (PCP), in human immunodeficiency virus-infected patients, but the mechanism of...
Thymidylate synthase binds to c-myc RNA in human colon cancer cells and in vitro.
Chu, E, Takechi, T, Jones, K L, Voeller, D M, Copur, S M, Maley, G F, ...
Using an immunoprecipitation-reverse transcription-PCR technique, we characterized a thymidylate synthase (TS) ribonucleoprotein complex in cultured human colon cancer cells that consists of TS...
Interaction of sulfonamide and sulfone compounds with Toxoplasma gondii dihydropteroate synthase.
Allegra, C J, Boarman, D, Kovacs, J A, Morrison, P, Beaver, J, Chabner, B A, ...
Toxoplasma gondii is a common protozoan disease that often causes life-threatening disease, particularly among patients with the acquired immunodeficiency syndrome. This study demonstrates that the...
Voeller, D M, Changchien, L M, Maley, G F, Maley, F, Takechi, T, Turner, R E, ...
Previous studies have shown that human TS mRNA translation is controlled by a negative autoregulatory mechanism. In this study, an RNA electrophoretic gel mobility shift assay confirmed a direct...
Identification of a thymidylate synthase ribonucleoprotein complex in human colon cancer cells.
Chu, E, Voeller, D M, Jones, K L, Takechi, T, Maley, G F, Maley, F, ...
Translation of thymidylate synthase (TS) mRNA is controlled by its own protein product, TS, in an autoregulatory manner. Direct binding of TS protein to two different cis-acting elements on the TS...
Allegra, C J, Drake, J C, Jolivet, J, Chabner, B A
We report the enhanced inhibitory potency of methotrexate (MTX) polyglutamates and dihydrofolate pentaglutamate on the catalytic activity of phosphoribosylaminoimidazolecarboxamide (AICAR)...
Polyglutamation of methotrexate. Is methotrexate a prodrug?
Chabner, B A, Allegra, C J, Curt, G A, Clendeninn, N J, Baram, J, Koizumi, S, ...
Potent in vitro and in vivo antitoxoplasma activity of the lipid-soluble antifolate trimetrexate.
Allegra, C J, Kovacs, J A, Drake, J C, Swan, J C, Chabner, B A, Masur, H
Trimetrexate, a highly lipid-soluble quinazoline antifolate now undergoing trials as an anticancer agent, was found to be a potent inhibitor of the dihydrofolate reductase (DHFR) isolated from...
Baram, J, Allegra, C J, Fine, R L, Chabner, B A
We investigated the effects of the antifolate methotrexate on intracellular folate pools of human myeloid precursor cells (MPCs). Immature MPCs, representing 3.2% of the original marrow population,...
Identification of an RNA binding site for human thymidylate synthase.
Chu, E, Voeller, D, Koeller, D M, Drake, J C, Takimoto, C H, Maley, G F, ...
Previous studies from this laboratory have shown that human TS mRNA translation is regulated by its protein product in a negative autoregulatory manner. In this paper, we identify an RNA binding site...
Autoregulation of human thymidylate synthase messenger RNA translation by thymidylate synthase.
Chu, E, Koeller, D M, Casey, J L, Drake, J C, Chabner, B A, Elwood, P C, ...
Thymidylate synthase (TS; 5,10-methylenetetrahydrofolate:dUMP C-methyltransferase, EC 2.1.1.45) is essential for the de novo synthesis of thymidylate, a precursor of DNA. Previous studies have shown...
Identification of in vivo target RNA sequences bound by thymidylate synthase.
Chu, E, Cogliati, T, Copur, S M, Borre, A, Voeller, D M, Allegra, C J, ...
We developed an immunoprecipitation-RNA-random PCR (rPCR) method to isolate cellular RNA sequences that bind to the folate-dependent enzyme thymidylate synthase (TS). Using this approach, nine...
Bioavailability of oral trimetrexate in patients with acquired immunodeficiency syndrome.
Rogers, P, Allegra, C J, Murphy, R F, Drake, J C, Masur, H, Poplack, D G, ...
The combination of the lipophilic antifolate trimetrexate and the rescue agent leucovorin has shown promise in the treatment of Pneumocystis carinii pneumonia in patients with acquired...
Potent antipneumocystis and antitoxoplasma activities of piritrexim, a lipid-soluble antifolate.
Kovacs, J A, Allegra, C J, Swan, J C, Drake, J C, Parrillo, J E, Chabner, B A, ...
Piritrexim, a lipid-soluble antifolate, was evaluated for its activity against Pneumocystis carinii and Toxoplasma gondii. The concentration of piritrexim needed to inhibit 50% of the catalytic...
Mechanism of pyrimethamine resistance in recent isolates of Plasmodium falciparum.
McCutchan, T F, Welsh, J A, Dame, J B, Quakyi, I A, Graves, P M, Drake, J C, ...
Clones of Plasmodium falciparum prepared from recent isolates of infected blood were studied to determine the molecular mechanism of naturally occurring pyrimethamine resistance. Total DNA, as well...
Kovacs, J A, Powell, F, Voeller, D, Allegra, C J
Isoprinosine has been reported to decrease progression to AIDS, primarily by preventing Pneumocystis carinii pneumonia (PCP), in human immunodeficiency virus-infected patients, but the mechanism of...
Thymidylate synthase binds to c-myc RNA in human colon cancer cells and in vitro.
Chu, E, Takechi, T, Jones, K L, Voeller, D M, Copur, S M, Maley, G F, ...
Using an immunoprecipitation-reverse transcription-PCR technique, we characterized a thymidylate synthase (TS) ribonucleoprotein complex in cultured human colon cancer cells that consists of TS...
Interaction of sulfonamide and sulfone compounds with Toxoplasma gondii dihydropteroate synthase.
Allegra, C J, Boarman, D, Kovacs, J A, Morrison, P, Beaver, J, Chabner, B A, ...
Toxoplasma gondii is a common protozoan disease that often causes life-threatening disease, particularly among patients with the acquired immunodeficiency syndrome. This study demonstrates that the...
Voeller, D M, Changchien, L M, Maley, G F, Maley, F, Takechi, T, Turner, R E, ...
Previous studies have shown that human TS mRNA translation is controlled by a negative autoregulatory mechanism. In this study, an RNA electrophoretic gel mobility shift assay confirmed a direct...
Identification of a thymidylate synthase ribonucleoprotein complex in human colon cancer cells.
Chu, E, Voeller, D M, Jones, K L, Takechi, T, Maley, G F, Maley, F, ...
Translation of thymidylate synthase (TS) mRNA is controlled by its own protein product, TS, in an autoregulatory manner. Direct binding of TS protein to two different cis-acting elements on the TS...
Allegra, C J, Drake, J C, Jolivet, J, Chabner, B A
We report the enhanced inhibitory potency of methotrexate (MTX) polyglutamates and dihydrofolate pentaglutamate on the catalytic activity of phosphoribosylaminoimidazolecarboxamide (AICAR)...
Polyglutamation of methotrexate. Is methotrexate a prodrug?
Chabner, B A, Allegra, C J, Curt, G A, Clendeninn, N J, Baram, J, Koizumi, S, ...
Potent in vitro and in vivo antitoxoplasma activity of the lipid-soluble antifolate trimetrexate.
Allegra, C J, Kovacs, J A, Drake, J C, Swan, J C, Chabner, B A, Masur, H
Trimetrexate, a highly lipid-soluble quinazoline antifolate now undergoing trials as an anticancer agent, was found to be a potent inhibitor of the dihydrofolate reductase (DHFR) isolated from...
Baram, J, Allegra, C J, Fine, R L, Chabner, B A
We investigated the effects of the antifolate methotrexate on intracellular folate pools of human myeloid precursor cells (MPCs). Immature MPCs, representing 3.2% of the original marrow population,...