Construction and expression of the complete Clostridium difficile toxin A gene in Escherichia coli.
Phelps, C J, Lyerly, D L, Johnson, J L, Wilkins, T D
Cloned fragments constituting the 8.1-kb toxin A gene of Clostridium difficile were used to reconstruct the intact gene. The recombinant toxin expressed in Escherichia coli was cytotoxic,...
Molecular characterization of the Clostridium difficile toxin A gene.
Dove, C H, Wang, S Z, Price, S B, Phelps, C J, Lyerly, D M, Wilkins, T D, ...
The gene encoding the toxin A protein of Clostridium difficile (strain VPI 10463) was cloned and sequenced. The coding region of 8,133 base pairs had a mol% G + C of 26.9 and encodes 2,710 amino...
Characterization of toxins A and B of Clostridium difficile with monoclonal antibodies.
Lyerly, D M, Phelps, C J, Toth, J, Wilkins, T D
Two monoclonal antibodies (MAbs) were used to learn more about the structures of Clostridium difficile toxins A and B. One of the antibodies, the PCG-4 MAb, reacted specifically with toxin A. This...
Monoclonal and specific polyclonal antibodies for immunoassay of Clostridium difficile toxin A.
Lyerly, D M, Phelps, C J, Wilkins, T D
Monoclonal antibody, affinity-purified antibody, and monospecific antiserum against toxin A were produced. The monoclonal antibody was an immunoglobulin G2a kappa chain isotype that...
Nucleotide sequence of Clostridium difficile toxin B gene.
Barroso, L A, Wang, S Z, Phelps, C J, Johnson, J L, Wilkins, T D
Construction and expression of the complete Clostridium difficile toxin A gene in Escherichia coli.
Phelps, C J, Lyerly, D L, Johnson, J L, Wilkins, T D
Cloned fragments constituting the 8.1-kb toxin A gene of Clostridium difficile were used to reconstruct the intact gene. The recombinant toxin expressed in Escherichia coli was cytotoxic,...
Molecular characterization of the Clostridium difficile toxin A gene.
Dove, C H, Wang, S Z, Price, S B, Phelps, C J, Lyerly, D M, Wilkins, T D, ...
The gene encoding the toxin A protein of Clostridium difficile (strain VPI 10463) was cloned and sequenced. The coding region of 8,133 base pairs had a mol% G + C of 26.9 and encodes 2,710 amino...
Characterization of toxins A and B of Clostridium difficile with monoclonal antibodies.
Lyerly, D M, Phelps, C J, Toth, J, Wilkins, T D
Two monoclonal antibodies (MAbs) were used to learn more about the structures of Clostridium difficile toxins A and B. One of the antibodies, the PCG-4 MAb, reacted specifically with toxin A. This...
Monoclonal and specific polyclonal antibodies for immunoassay of Clostridium difficile toxin A.
Lyerly, D M, Phelps, C J, Wilkins, T D
Monoclonal antibody, affinity-purified antibody, and monospecific antiserum against toxin A were produced. The monoclonal antibody was an immunoglobulin G2a kappa chain isotype that...
Nucleotide sequence of Clostridium difficile toxin B gene.
Barroso, L A, Wang, S Z, Phelps, C J, Johnson, J L, Wilkins, T D
Khodr, C E, Hurley, D L, Phelps, C J
The hypothalamic tuberoinfundibular dopaminergic (TIDA) neurones secrete dopamine, which inhibits prolactin secretion. TIDA neurone numbers are deficient in Ames (df/df) and Snell (dw/dw) dwarf mice,...