C. Jane McGlade

Cluster analysis of protein array results via similarity of Gene Ontology annotation (2006)

Wolting, Cheryl, McGlade, C Jane, Tritchler, David

Abstract Background With the advent of high-throughput proteomic experiments such as arrays of purified proteins comes the need to analyse sets of proteins as an ensemble, as opposed to the...

Phosphatidylinositol 3'-kinase and MAPK/ERK kinase 1/2 differentially regulate expression of vascular endothelial growth factor in human malignant astrocytoma cells (2002)

Woods, Stacy A., McGlade, C. Jane, Guha, Abhijit

Malignant astrocytomas are characterized by extensive vascularization attributed to increased expression of the angiogenic cytokine vascular endothelial growth factor (VEGF). VEGF is elevated in...

LNX functions as a RING type E3 ubiquitin ligase that targets the cell fate determinant Numb for ubiquitin-dependent degradation

Nie, Jing, McGill, Melanie A., Dermer, Matt, Dho, Sascha E., Wolting, Cheryl D., McGlade, C.Jane

LNX is a RING finger and PDZ domain containing protein that interacts with the cell fate determinant Numb. To investigate the function of LNX, we tested its RING finger domain for ubiquitin ligase...

Functional Cooperation between c-Cbl and Src-Like Adaptor Protein 2 in the Negative Regulation of T-Cell Receptor Signaling

Loreto, Michael P., Berry, Donna M., McGlade, C. Jane

Adaptor proteins assemble multiprotein signaling complexes, enabling the transduction of intracellular signals. While many adaptor proteins positively regulate signaling in this manner, a subgroup of...

Gads/Grb2-Mediated Association with LAT Is Critical for the Inhibitory Function of Gab2 in T Cells

Yamasaki, Sho, Nishida, Keigo, Sakuma, Machie, Berry, Donna, McGlade, C. Jane, Hirano, Toshio, ...

A docking protein, Gab2, is recruited to the vicinity of the TCR complex and inhibits downstream signaling by interaction with negative regulators. However, the molecular mechanisms of this...

Integrin β cytoplasmic domain interactions with phosphotyrosine-binding domains: A structural prototype for diversity in integrin signaling

Calderwood, David A., Fujioka, Yosuke, De Pereda, Jose M., García-Alvarez, Begoña, Nakamoto, Tetsuya, Margolis, Ben, ...

The cytoplasmic domains (tails) of heterodimeric integrin adhesion receptors mediate integrins' biological functions by binding to cytoplasmic proteins. Most integrin β tails contain one or two...

The Cell Fate Determinant Numb Interacts with EHD/Rme-1 Family Proteins and Has a Role in Endocytic Recycling

Smith, Christian A., Dho, Sascha E., Donaldson, Julie, Tepass, Ulrich, McGlade, C. Jane

The adaptor protein Numb is necessary for the cell fate specification of progenitor cells in the Drosophila nervous system. Numb is evolutionarily conserved and previous studies have provided...

T-Cell Protein Tyrosine Phosphatase (Tcptp) Is a Negative Regulator of Colony-Stimulating Factor 1 Signaling and Macrophage Differentiation

Simoncic, Paul D., Bourdeau, Annie, Lee-Loy, Ailsa, Rohrschneider, Larry R., Tremblay, Michel L., Stanley, E. Richard, ...

Mice null for the T-cell protein tyrosine phosphatase (Tcptp−/−) die shortly after birth due to complications arising from the development of a systemic inflammatory disease. It was originally...

Dynamic Regulation of Mammalian Numb by G Protein-coupled Receptors and Protein Kinase C Activation: Structural Determinants of Numb Association with the Cortical Membrane

Dho, Sascha E., Trejo, JoAnn, Siderovski, David P., McGlade, C. Jane

The cell fate determinant Numb is a membrane-associated adaptor protein involved in both development and intracellular vesicular trafficking. It has a phosphotyrosine-binding (PTB) domain and...

LNX functions as a RING type E3 ubiquitin ligase that targets the cell fate determinant Numb for ubiquitin-dependent degradation

Nie, Jing, McGill, Melanie A., Dermer, Matt, Dho, Sascha E., Wolting, Cheryl D., McGlade, C.Jane

LNX is a RING finger and PDZ domain containing protein that interacts with the cell fate determinant Numb. To investigate the function of LNX, we tested its RING finger domain for ubiquitin ligase...

Functional Cooperation between c-Cbl and Src-Like Adaptor Protein 2 in the Negative Regulation of T-Cell Receptor Signaling

Loreto, Michael P., Berry, Donna M., McGlade, C. Jane

Adaptor proteins assemble multiprotein signaling complexes, enabling the transduction of intracellular signals. While many adaptor proteins positively regulate signaling in this manner, a subgroup of...

Gads/Grb2-Mediated Association with LAT Is Critical for the Inhibitory Function of Gab2 in T Cells

Yamasaki, Sho, Nishida, Keigo, Sakuma, Machie, Berry, Donna, McGlade, C. Jane, Hirano, Toshio, ...

A docking protein, Gab2, is recruited to the vicinity of the TCR complex and inhibits downstream signaling by interaction with negative regulators. However, the molecular mechanisms of this...

Integrin β cytoplasmic domain interactions with phosphotyrosine-binding domains: A structural prototype for diversity in integrin signaling

Calderwood, David A., Fujioka, Yosuke, De Pereda, Jose M., García-Alvarez, Begoña, Nakamoto, Tetsuya, Margolis, Ben, ...

The cytoplasmic domains (tails) of heterodimeric integrin adhesion receptors mediate integrins' biological functions by binding to cytoplasmic proteins. Most integrin β tails contain one or two...

The Cell Fate Determinant Numb Interacts with EHD/Rme-1 Family Proteins and Has a Role in Endocytic Recycling

Smith, Christian A., Dho, Sascha E., Donaldson, Julie, Tepass, Ulrich, McGlade, C. Jane

The adaptor protein Numb is necessary for the cell fate specification of progenitor cells in the Drosophila nervous system. Numb is evolutionarily conserved and previous studies have provided...

T-Cell Protein Tyrosine Phosphatase (Tcptp) Is a Negative Regulator of Colony-Stimulating Factor 1 Signaling and Macrophage Differentiation

Simoncic, Paul D., Bourdeau, Annie, Lee-Loy, Ailsa, Rohrschneider, Larry R., Tremblay, Michel L., Stanley, E. Richard, ...

Mice null for the T-cell protein tyrosine phosphatase (Tcptp−/−) die shortly after birth due to complications arising from the development of a systemic inflammatory disease. It was originally...

Dynamic Regulation of Mammalian Numb by G Protein-coupled Receptors and Protein Kinase C Activation: Structural Determinants of Numb Association with the Cortical Membrane

Dho, Sascha E., Trejo, JoAnn, Siderovski, David P., McGlade, C. Jane

The cell fate determinant Numb is a membrane-associated adaptor protein involved in both development and intracellular vesicular trafficking. It has a phosphotyrosine-binding (PTB) domain and...

Phosphatidylinositol 3'-kinase and MAPK/ERK kinase 1/2 differentially regulate expression of vascular endothelial growth factor in human malignant astrocytoma cells.

Woods, Stacy A., McGlade, C. Jane, Guha, Abhijit

Malignant astrocytomas are characterized by extensive vascularization attributed to increased expression of the angiogenic cytokine vascular endothelial growth factor (VEGF). VEGF is elevated in...

aPKC-mediated phosphorylation regulates asymmetric membrane localization of the cell fate determinant Numb

Smith, Christian A, Lau, Kimberly M, Rahmani, Zohra, Dho, Sascha E, Brothers, Greg, She, Ye Min, ...

In Drosophila, the partition defective (Par) complex containing Par3, Par6 and atypical protein kinase C (aPKC) directs the polarized distribution and unequal segregation of the cell fate determinant...

Efficient T-cell receptor signaling requires a high-affinity interaction between the Gads C-SH3 domain and the SLP-76 RxxK motif

Seet, Bruce T, Berry, Donna M, Maltzman, Jonathan S, Shabason, Jacob, Raina, Monica, Koretzky, Gary A, ...

The relationship between the binding affinity and specificity of modular interaction domains is potentially important in determining biological signaling responses. In signaling from the T-cell...

The SH3–SAM Adaptor HACS1 is Up-regulated in B Cell Activation Signaling Cascades

Zhu, Yuan Xiao, Benn, Sally, Li, Zhi Hua, Wei, Ellen, Masih-Khan, Esther, Trieu, Young, ...

HACS1 is a Src homology 3 and sterile alpha motif domain–containing adaptor that is preferentially expressed in normal hematopoietic tissues and malignancies including myeloid leukemia, lymphoma,...

T cell receptor ligation induces the formation of dynamically regulated signaling assemblies

Bunnell, Stephen C., Hong, David I., Kardon, Julia R., Yamazaki, Tetsuo, McGlade, C. Jane, Barr, Valarie A., ...

Tcell antigen receptor (TCR) ligation initiates tyrosine kinase activation, signaling complex assembly, and immune synapse formation. Here, we studied the kinetics and mechanics of signaling complex...