C. M. Thomas

Publication List Details

Period

1985 - 2005

Number

80

Co-Authors

Validation of Vincenty's Formulas for the Geodesic Using a New Fourth-Order Extension of Kivioja's Formula (2005)

Thomas, C.M., Featherstone, W.E.

Vincenty's (1975) formulas for the direct and inverse geodetic problems (i.e., in relation to the geodesic) have been verified by comparing them with a new formula developed by adapting a...

Validation of Vincenty's Formulas for the Geodesic Using a New Fourth-Order Extension of Kivioja's Formula (2005)

Thomas, C.M., Featherstone, W.E.

Vincenty's (1975) formulas for the direct and inverse geodetic problems (i.e., in relation to the geodesic) have been verified by comparing them with a new formula developed by adapting a...

SYSTEM IMPROVEMENT USING SIGNAL-TO-NOISE RATIO ESTIMATION. (2005)

Thomas, C. M., Gagliardi, R. M.

This report presents the results of a study effort to determine the possibility of improving the performance of space vehicle tracking and detection systems by using signal-to-noise ratio (SNR)...

Validation of Vincenty's Formulas for the Geodesic Using a New Fourth-Order Extension of Kivioja's Formula (2005)

Thomas, C.M., Featherstone, W.E.

Vincenty's (1975) formulas for the direct and inverse geodetic problems (i.e., in relation to the geodesic) have been verified by comparing them with a new formula developed by adapting a...

Gene shuffling-generated and natural variants of the tomato resistance gene Cf-9 exhibit different auto-necrosis-inducing activities in Nicotiana species (2004)

Wulff, B.B.H., Kruijt, M., Collins, P.L., Thomas, C.M., Ludwig, A.A., ...

Tomato Cf genes encode membrane-bound proteins with extracellular leucine-rich repeats, and confer resistance to the fungal tomato pathogen Cladosporium fulvum, and a hypersensitive response (HR) to...

The complete nucleotide sequence and environmental distribution of the cryptic, conjugative, broad-host-range plasmid pIPO2 islated from bacteria of the wheat rhizosphere (2002)

Tauch, A., Schneiker, S., Selbitschka, W., PÜhler, A., Smalla, K., ...

The flood of sequence data resulting from the large number of current genome projects has increased the need for a flexible, open source genome annotation system, which so far has not existed. To...

Functional dissection of the ParB homologue (KorB) from IncP-1 plasmid RK2 (2002)

Lukaszewicz, M., Kostelidou, K., Bartosik, A. A., Cooke, G. D., Thomas, C. M., Jagura-Burdzy, G.

Active partitioning of low-copy number plasmids requires two proteins belonging to the ParA and ParB families and a cis-acting site which ParB acts upon. Active separation of clusters of plasmid...

Dispersion of Cf-4 in Lycopersicon germplasm (2000)

Haanstra, J.P.W., Thomas, C.M., Jones, J.D.G., Lindhout, P.

In the past, numerous Cf genes have been reported in tomato (Lycopersicon esculentum Mill.) that confer resistance against leaf mould (Cladosporium fulvum Cke.). We are interested in genetic...

Isolation of a fraction from cauliflower mosaic virus-infected protoplasts which is active in the synthesis of (+) and (-) strand viral DNA and reverse transcription of primed RNA templates (1985)

Thomas, C.M., Hull, R., Bryant, J.A., Maule, A.J.

Sub-cellular fractions, isolated from cauliflower mosaic virus (CaMV)-infected turnip protoplasts, are capable of syn-thesising CaMV DNA in vitro on an endogenous template and of reverse transcribing...

KorA protein of promiscuous plasmid RK2 controls a transcriptional switch between divergent operons for plasmid replication and conjugative transfer.

Jagura-Burdzy, G, Thomas, C M

The trfA and trb operons, encoding genes essential for replication and conjugative transfer of broad host range plasmid RK2, are transcribed divergently. Deletion analysis presented here indicates...

Functional dissection of the ParB homologue (KorB) from IncP-1 plasmid RK2

Lukaszewicz, M., Kostelidou, K., Bartosik, A. A., Cooke, G. D., Thomas, C. M., Jagura-Burdzy, G.

Active partitioning of low-copy number plasmids requires two proteins belonging to the ParA and ParB families and a cis-acting site which ParB acts upon. Active separation of clusters of plasmid...

Transposon tagging of the Defective embryo and meristems gene of tomato.

Keddie, J S, Carroll, B J, Thomas, C M, Reyes, M E, Klimyuk, V, Holtan, H, ...

The shoot and root apical meristems (SAMs and RAMs, respectively) of higher plants are mechanistically and structurally similar. This has led previously to the suggestion that the SAM and RAM...

Characterization of the tomato Cf-4 gene for resistance to Cladosporium fulvum identifies sequences that determine recognitional specificity in Cf-4 and Cf-9.

Thomas, C M, Jones, D A, Parniske, M, Harrison, K, Balint-Kurti, P J, Hatzixanthis, K, ...

In many interactions between plants and their pathogens, resistance to infection is specified by plant resistance (R) genes and corresponding pathogen avirulence (Avr) genes. In tomato, the Cf-4 and...

Transcriptional and translational control of the genes for the mating pair formation apparatus of promiscuous IncP plasmids.

Zatyka, M, Jagura-Burdzy, G, Thomas, C M

The trb operon of broad-host-range plasmid RK2 encodes most of the genes required for formation of mating-pair apparatus and is thus essential for the promiscuous spread of this plasmid. Only two...

The korF region of broad-host-range plasmid RK2 encodes two polypeptides with transcriptional repressor activity.

Jagura-Burdzy, G, Ibbotson, J P, Thomas, C M

Broad-host-range IncP plasmid RK2 possesses a series of operons involved in plasmid maintenance, whose expression is coordinated by a number of regulators, most of which are encoded in the central...

Transcriptional analysis, translational analysis, and sequence of the kilA-tellurite resistance region of plasmid RK2Ter.

Walter, E G, Thomas, C M, Ibbotson, J P, Taylor, D E

The tellurite resistance (Ter) determinant of the IncP alpha plasmid RK2Ter, a variant of RK2 (also called RP4), is located between the kilA and korA genes involved in plasmid replication control....

Regions of broad-host-range plasmid RK2 which are essential for replication and maintenance.

Thomas, C M, Meyer, R, Helinski, D R

The sites of cleavage on the map of the broad-host-range plasmid RK2 (56 kilobases) were determined for the BglII, PstI, and SmaI restriction enzymes, and the determinants for tetracycline and...

Nucleotide sequence of the transcriptional repressor gene korB which plays a key role in regulation of the copy number of broad host range plasmid RK2.

Theophilus, B D, Thomas, C M

The product of the korB gene of broad host range plasmid RK2 is one of at least two proteins which repress transcription of the essential replication gene trfA. We report here the nucleotide sequence...

Multifunctional repressor KorB can block transcription by preventing isomerization of RNA polymerase-promoter complexes.

Williams, D R, Motallebi-Veshareh, M, Thomas, C M

The KorB protein of broad-host-range plasmid RK2 is a transcriptional repressor involved in the control of genes for plasmid replication, conjugative transfer and stable maintenance. We have purified...

The trfB region of broad host range plasmid RK2: the nucleotide sequence reveals incC and key regulatory gene trfB/korA/korD as overlapping genes.

Thomas, C M, Smith, C A

We report the nucleotide sequence of the trfB region of broad host range plasmid RK2. This region encodes the following loci: trfB, identical to korA and korD, which encodes a key transcriptional...

The nucleotide sequence and transcription of minicircular mitochondrial DNA's associated with male-fertile and cytoplasmic male-sterile lines of sugarbeet.

Thomas, C M

Male-fertile (MF) and cytoplasmic male-sterile (CMS) lines of sugarbeet possess characteristic low molecular weight minicircular mitochondrial DNA's, and Southern hybridizations revealed sequence...

The trfA and trfB promoter regions of broad host range plasmid RK2 share common potential regulatory sequences.

Smith, C A, Shingler, V, Thomas, C M

The positions of the trfA and trfB promoters of broad host range IncP plasmid RK2 (identical to RP1, RP4, R68 and R18 ) were identified by RNA polymerase protection studies, and the nucleotide...

Isolation of a fraction from cauliflower mosaic virus-infected protoplasts which is active in the synthesis of (+) and (-) strand viral DNA and reverse transcription of primed RNA templates.

Thomas, C M, Hull, R, Bryant, J A, Maule, A J

Sub-cellular fractions, isolated from cauliflower mosaic virus (CaMV)-infected turnip protoplasts, are capable of synthesising CaMV DNA in vitro on an endogenous template and of reverse transcribing...

Evidence from cauliflower mosaic virus virion DNA for additional discontinuities in the plus strand.

Maule, A J, Thomas, C M

Two-dimensional electrophoresis of cauliflower mosaic virus (CaMV) virion DNA and analysis of Southern blots using (+) strand-specific probes to the 5' termini of the beta (5.4 Kb) and alpha (2.6 Kb)...

Regulation of the trfA and trfB promoters of broad host range plasmid RK2: identification of sequences essential for regulation by trfB/korA/korD.

Theophilus, B D, Cross, M A, Smith, C A, Thomas, C M

Using a plasmid containing a transcriptional fusion in which the E.coli galK gene is expressed from the trfB promoter of broad host range plasmid RK2 we show that transcription from the trfB promoter...

Crosstalk between plasmid vegetative replication and conjugative transfer: repression of the trfA operon by trbA of broad host range plasmid RK2.

Jagura-Burdzy, G, Khanim, F, Smith, C A, Thomas, C M

Previous deletion and complementation analysis has indicated that the region between trfA and kilBI (trbB) encodes trans-acting factor, designated trbA, required for conjugative transfer of broad...

Gene regulation on broad host range plasmid RK2: identification of three novel operons whose transcription is repressed by both KorA and KorC.

Thomas, C M, Ibbotson, J P, Wang, N Y, Smith, C A, Tipping, R, Loader, N M

The product of the korA gene of broad host range plasmid RK2 is a key transcriptional repressor which regulates not only the expression of the essential replication gene trfA but also its own...

Comparison of the nucleotide sequences of the vegetative replication origins of broad host range IncP plasmids R751 and RK2 reveals conserved features of probable functional importance.

Smith, C A, Thomas, C M

An 864 bp EcoRI fragment carrying oriVR751, the vegetative replication origin of broad host range IncP plasmid R751, was cloned and sequenced. Only the trfA gene of the IncP plasmid RK2 was required...

Absence of prolactin gene expression in colorectal cancer.

Wood, A J, Thomas, C M, Baumforth, K R, Flavell, J R, Scott, K W, Grace, R H, ...

AIMS: Previous studies documenting hyperprolactinaemia in patients with colorectal cancer have suggested that the tumour is the source of hormone production. The aim of this study was to determine...

Analysis of copy number control elements in the region of the vegetative replication origin of the broad host range plasmid RK2.

Thomas, C M, Cross, M A, Hussain, A A, Smith, C A

Broad host-range plasmid RK2 is able to replicate in a controlled manner in most Gram negative bacterial species. To analyze the elements of its control mechanism, we have measured the copy number in...

The korB gene of broad host range plasmid RK2 is a major copy number control element which may act together with trfB by limiting trfA expression.

Thomas, C M, Hussain, A A

For replication, plasmid RK2 encodes a vegetative replication origin, oriV RK2, and a gene, trfA, whose polypeptide product(s) is essential for oriV RK2 activity. The trfA gene is transcribed as part...

Germinal Transpositions of the Maize Element Dissociation from T-DNA Loci in Tomato

Carroll, B. J., Klimyuk, V. I., Thomas, C. M., Bishop, G. J., Harrison, K., Scofield, S. R., ...

We have analyzed the pattern of germinal transpositions of artificial Dissociation (Ds) transposons in tomato. T-DNA constructs carrying Ds were transformed into tomato, and the elements were...

Distribution of Unlinked Transpositions of a Ds Element from a T-DNA Locus on Tomato Chromosome 4

Briza, J., Carroll, B. J., Klimyuk, V. I., Thomas, C. M., Jones, D. A., Jones, JDG.

In maize, receptor sites for unlinked transpositions of Activator (Ac) elements are not distributed randomly. To test whether the same is true in tomato, the receptor sites for a Dissociation (Ds)...

KorA protein of promiscuous plasmid RK2 controls a transcriptional switch between divergent operons for plasmid replication and conjugative transfer.

Jagura-Burdzy, G, Thomas, C M

The trfA and trb operons, encoding genes essential for replication and conjugative transfer of broad host range plasmid RK2, are transcribed divergently. Deletion analysis presented here indicates...

Functional dissection of the ParB homologue (KorB) from IncP-1 plasmid RK2

Lukaszewicz, M., Kostelidou, K., Bartosik, A. A., Cooke, G. D., Thomas, C. M., Jagura-Burdzy, G.

Active partitioning of low-copy number plasmids requires two proteins belonging to the ParA and ParB families and a cis-acting site which ParB acts upon. Active separation of clusters of plasmid...

Transposon tagging of the Defective embryo and meristems gene of tomato.

Keddie, J S, Carroll, B J, Thomas, C M, Reyes, M E, Klimyuk, V, Holtan, H, ...

The shoot and root apical meristems (SAMs and RAMs, respectively) of higher plants are mechanistically and structurally similar. This has led previously to the suggestion that the SAM and RAM...

Characterization of the tomato Cf-4 gene for resistance to Cladosporium fulvum identifies sequences that determine recognitional specificity in Cf-4 and Cf-9.

Thomas, C M, Jones, D A, Parniske, M, Harrison, K, Balint-Kurti, P J, Hatzixanthis, K, ...

In many interactions between plants and their pathogens, resistance to infection is specified by plant resistance (R) genes and corresponding pathogen avirulence (Avr) genes. In tomato, the Cf-4 and...

Transcriptional and translational control of the genes for the mating pair formation apparatus of promiscuous IncP plasmids.

Zatyka, M, Jagura-Burdzy, G, Thomas, C M

The trb operon of broad-host-range plasmid RK2 encodes most of the genes required for formation of mating-pair apparatus and is thus essential for the promiscuous spread of this plasmid. Only two...

The korF region of broad-host-range plasmid RK2 encodes two polypeptides with transcriptional repressor activity.

Jagura-Burdzy, G, Ibbotson, J P, Thomas, C M

Broad-host-range IncP plasmid RK2 possesses a series of operons involved in plasmid maintenance, whose expression is coordinated by a number of regulators, most of which are encoded in the central...

Transcriptional analysis, translational analysis, and sequence of the kilA-tellurite resistance region of plasmid RK2Ter.

Walter, E G, Thomas, C M, Ibbotson, J P, Taylor, D E

The tellurite resistance (Ter) determinant of the IncP alpha plasmid RK2Ter, a variant of RK2 (also called RP4), is located between the kilA and korA genes involved in plasmid replication control....

Regions of broad-host-range plasmid RK2 which are essential for replication and maintenance.

Thomas, C M, Meyer, R, Helinski, D R

The sites of cleavage on the map of the broad-host-range plasmid RK2 (56 kilobases) were determined for the BglII, PstI, and SmaI restriction enzymes, and the determinants for tetracycline and...

Nucleotide sequence of the transcriptional repressor gene korB which plays a key role in regulation of the copy number of broad host range plasmid RK2.

Theophilus, B D, Thomas, C M

The product of the korB gene of broad host range plasmid RK2 is one of at least two proteins which repress transcription of the essential replication gene trfA. We report here the nucleotide sequence...

Multifunctional repressor KorB can block transcription by preventing isomerization of RNA polymerase-promoter complexes.

Williams, D R, Motallebi-Veshareh, M, Thomas, C M

The KorB protein of broad-host-range plasmid RK2 is a transcriptional repressor involved in the control of genes for plasmid replication, conjugative transfer and stable maintenance. We have purified...

The trfB region of broad host range plasmid RK2: the nucleotide sequence reveals incC and key regulatory gene trfB/korA/korD as overlapping genes.

Thomas, C M, Smith, C A

We report the nucleotide sequence of the trfB region of broad host range plasmid RK2. This region encodes the following loci: trfB, identical to korA and korD, which encodes a key transcriptional...

The nucleotide sequence and transcription of minicircular mitochondrial DNA's associated with male-fertile and cytoplasmic male-sterile lines of sugarbeet.

Thomas, C M

Male-fertile (MF) and cytoplasmic male-sterile (CMS) lines of sugarbeet possess characteristic low molecular weight minicircular mitochondrial DNA's, and Southern hybridizations revealed sequence...

The trfA and trfB promoter regions of broad host range plasmid RK2 share common potential regulatory sequences.

Smith, C A, Shingler, V, Thomas, C M

The positions of the trfA and trfB promoters of broad host range IncP plasmid RK2 (identical to RP1, RP4, R68 and R18 ) were identified by RNA polymerase protection studies, and the nucleotide...

Isolation of a fraction from cauliflower mosaic virus-infected protoplasts which is active in the synthesis of (+) and (-) strand viral DNA and reverse transcription of primed RNA templates.

Thomas, C M, Hull, R, Bryant, J A, Maule, A J

Sub-cellular fractions, isolated from cauliflower mosaic virus (CaMV)-infected turnip protoplasts, are capable of synthesising CaMV DNA in vitro on an endogenous template and of reverse transcribing...

Evidence from cauliflower mosaic virus virion DNA for additional discontinuities in the plus strand.

Maule, A J, Thomas, C M

Two-dimensional electrophoresis of cauliflower mosaic virus (CaMV) virion DNA and analysis of Southern blots using (+) strand-specific probes to the 5' termini of the beta (5.4 Kb) and alpha (2.6 Kb)...

Regulation of the trfA and trfB promoters of broad host range plasmid RK2: identification of sequences essential for regulation by trfB/korA/korD.

Theophilus, B D, Cross, M A, Smith, C A, Thomas, C M

Using a plasmid containing a transcriptional fusion in which the E.coli galK gene is expressed from the trfB promoter of broad host range plasmid RK2 we show that transcription from the trfB promoter...

Crosstalk between plasmid vegetative replication and conjugative transfer: repression of the trfA operon by trbA of broad host range plasmid RK2.

Jagura-Burdzy, G, Khanim, F, Smith, C A, Thomas, C M

Previous deletion and complementation analysis has indicated that the region between trfA and kilBI (trbB) encodes trans-acting factor, designated trbA, required for conjugative transfer of broad...

Gene regulation on broad host range plasmid RK2: identification of three novel operons whose transcription is repressed by both KorA and KorC.

Thomas, C M, Ibbotson, J P, Wang, N Y, Smith, C A, Tipping, R, Loader, N M

The product of the korA gene of broad host range plasmid RK2 is a key transcriptional repressor which regulates not only the expression of the essential replication gene trfA but also its own...

Comparison of the nucleotide sequences of the vegetative replication origins of broad host range IncP plasmids R751 and RK2 reveals conserved features of probable functional importance.

Smith, C A, Thomas, C M

An 864 bp EcoRI fragment carrying oriVR751, the vegetative replication origin of broad host range IncP plasmid R751, was cloned and sequenced. Only the trfA gene of the IncP plasmid RK2 was required...

Absence of prolactin gene expression in colorectal cancer.

Wood, A J, Thomas, C M, Baumforth, K R, Flavell, J R, Scott, K W, Grace, R H, ...

AIMS: Previous studies documenting hyperprolactinaemia in patients with colorectal cancer have suggested that the tumour is the source of hormone production. The aim of this study was to determine...

Analysis of copy number control elements in the region of the vegetative replication origin of the broad host range plasmid RK2.

Thomas, C M, Cross, M A, Hussain, A A, Smith, C A

Broad host-range plasmid RK2 is able to replicate in a controlled manner in most Gram negative bacterial species. To analyze the elements of its control mechanism, we have measured the copy number in...

The korB gene of broad host range plasmid RK2 is a major copy number control element which may act together with trfB by limiting trfA expression.

Thomas, C M, Hussain, A A

For replication, plasmid RK2 encodes a vegetative replication origin, oriV RK2, and a gene, trfA, whose polypeptide product(s) is essential for oriV RK2 activity. The trfA gene is transcribed as part...

Germinal Transpositions of the Maize Element Dissociation from T-DNA Loci in Tomato

Carroll, B. J., Klimyuk, V. I., Thomas, C. M., Bishop, G. J., Harrison, K., Scofield, S. R., ...

We have analyzed the pattern of germinal transpositions of artificial Dissociation (Ds) transposons in tomato. T-DNA constructs carrying Ds were transformed into tomato, and the elements were...

Distribution of Unlinked Transpositions of a Ds Element from a T-DNA Locus on Tomato Chromosome 4

Briza, J., Carroll, B. J., Klimyuk, V. I., Thomas, C. M., Jones, D. A., Jones, JDG.

In maize, receptor sites for unlinked transpositions of Activator (Ac) elements are not distributed randomly. To test whether the same is true in tomato, the receptor sites for a Dissociation (Ds)...

Genetic and molecular analysis of tomato Cf genes for resistance to Cladosporium fulvum.

Thomas, C M, Dixon, M S, Parniske, M, Golstein, C, Jones, J D

In many plant-pathogen interactions resistance to disease is controlled by the interaction of plant-encoded resistance (R) genes and pathogen-encoded avirulence (Avr) genes. The interaction between...

The effects of sulindac and indomethacin on the anti-hypertensive and diuretic action of hydrochlorothiazide in patients with mild to moderate essential hypertension.

Koopmans, P P, Thien, T, Thomas, C M, Van Den Berg, R J, Gribnau, F W

In this double-blind two period crossover study, we investigated the effect of indomethacin and sulindac on blood pressure in 25 hypertensive patients being treated with hydrochlorothiazide. The...

The phase of the menstrual cycle has no influence on the disease-free survival of patients with mammary carcinoma.

Wobbes, T., Thomas, C. M., Segers, M. F., Peer, P. G., Bruggink, E. D., Beex, L. V.

We evaluated the outcome of treatment for mammary carcinoma in 89 premenopausal women in relation to the phase of the menstrual cycle. The phase of the cycle was determined on the basis of serum...

Comparison of four serum tumour markers in the diagnosis of colorectal carcinoma.

Van Der Schouw, Y. T., Verbeek, A. L., Wobbes, T., Segers, M. F., Thomas, C. M.

The assessment of the diagnostic power of four serum tumour markers, CEA, CA 19-9, CA 50 and CA 195 for colorectal carcinoma is described, according to recently formulated guidelines. Preoperative...