Najimi, M, Caruso, M, Pauwelyn, K, Cantz, T, Forbes, S, ...
There has been recent progress in the isolation and characterisation of stem/progenitor cells that may differentiate towards the hepatic lineage. This has raised expectations that therapy of genetic...
Salesse, S, Delwel, R, Verfaillie, C M
The mechanism underlying p210(BCR/ABL) oncoprotein-mediated transformation in chronic myelogenous leukemia (CML) is not fully understood. We hypothesized that p210(BCR/ABL) suppresses expression of...
Chronic myelogenous leukemia (CML) is a malignancy of the human hematopoietic stem cell (HSC) caused by the p210BCR/ABL oncoprotein. Although alternative splicing of pre-mRNA is a critical...
Uno de los campos de la medicina que más expectativas han levantado en los últimos años es la terapia celular con células madre. El aislamiento de células embrionarias humanas, la aparente e...
Verfaillie, C.M., Prósper Cardoso, Felipe
Uno de los campos de la medicina que más expectativas han levantado en los últimos años es la terapia celular con células madre. El aislamiento de células embrionarias humanas, la aparente e...
One of the fields of medicine that has created the greatest expectations in recent years is cellular therapy with stem cells. The isolation of human embryo cells, the apparent and unexpected...
Chronic myelogenous leukemia: mechanisms underlying disease progression (2002)
Jahagirdar, B N, Verfaillie, C M
Chronic myelogenous leukemia (CML), characterized by the BCR-ABL gene rearrangement, has been extensively studied. Significant progress has been made in the area of BCR-ABL-mediated intracellular...
Leukemia in donor cells after allogeneic hematopoietic stem cell transplant (2002)
Hirsch, B A, Hammerschmidt, D, McGlennen, R C, Nguyen, P L, Verfaillie, C M
The development of leukemia in donor cells after allogeneic hematopoietic stem cell transplant is an extremely rare event. We report here the case of a patient who developed myelodysplastic...
Purification and ex vivo expansion of postnatal human marrow mesodermal progenitor cells (2001)
Lund, T, Lenvik, T, Aguiar, D, Koodie, L, Verfaillie, C M
It is here reported that mesenchymal stem cells known to give rise to limb-bud mesoderm can, at the single-cell level, also differentiate into cells of visceral mesoderm and can be expanded...
Mesenchymal stem cells were isolated and a subpopulation of cells--multipotent adult progenitor cells--were identified that have the potential for multilineage differentiation. Their ability to...
Almeida-Porada, G, Du, J, Lemischka, I R, Moore, K A, Zanjani, E D, ...
This report describes stroma-based and stroma-free cultures that maintain long-term engrafting hematopoietic cells for at least 14 days ex vivo. Umbilical cord blood (UCB) CD34(+) cells were cultured...
Novel therapies for chronic myelogenous leukemia (2001)
Miller, J S, Shet, A, Verfaillie, C M
The BCR-ABL oncogene is essential to the pathogenesis of chronic myelogenous leukemia, and immune mechanisms play an important role in control of this disease. Understanding of the molecular...
Most insights into the molecular mechanisms underlying transformation by the p210(BCR/ABL) oncoprotein are derived from studies in which BCR/ABL cDNA was introduced into hematopoietic or fibroblast...
DeFor, T E, McGlave, P B, Miller, J S, Verfaillie, C M, Wagner, J E, ...
PURPOSE: To determine if the favorable outcomes after transplantation of matched sibling donor bone marrow in patients with chronic myelogenous leukemia can be achieved using bone marrow from an...
Regulation of hematopoiesis through adhesion receptors (2001)
Normal steady-state hematopoiesis takes place in the bone marrow microenvironment. Soluble factors as well as contact interactions between the hematopoietic cells and the marrow microenvironment...
Current status of cord blood banking and transplantation in the United States and Europe (2001)
Broxmeyer, H E, McCullough, J, Piaciabello, W, Rebulla, P, Verfaillie, C M, ...
Cord blood (CB) transplantation has expanded the ability of the transplantation community to meet the growing needs of their patients. Clinical data over the last decade show promising results in CB...
Hirsch, B A, Miller, J S, McGlennen, R C, Verfaillie, C M, McGlave, P B, ...
Autologous reconstitution is the recovery of autologous hematopoietic function after failure of an allogeneic graft to establish sustained hematopoiesis either with or without preceding donor...
McGlave, P B, DeFor, T E, Miller, J S, Ramsay, N K, Verfaillie, C M, ...
We performed a case-control analysis of 42 patients with advanced leukemia or MDS comparing peripheral blood stem cell (PBSC) with marrow grafts (BMT) from HLA-matched sibling donors. PBSC were...
OBJECTIVE: The majority of studies assessing ex-vivo expansion of primitive hematopoietic cells only address production of myeloid progeny whereas it may be more appropriate to maintain or expand...
To develop a model of early human adult B lymphopoiesis, we cultured CD34+CD38+CD10+ pro-B cells in contact with AFT024 stroma in X-VIVO10 media with 5% serum. The cytokines FLT3L + SCF + IL7 + IGF1...
beta(1)-integrin engagement on normal (NL) CD34(+) cells increases levels of the cyclin-dependent kinase inhibitor (cdki), p27(Kip), decreases cdk2 activity, and inhibits G(1)/S-phase progression. In...
Hung, Y, Wissink, S D, Verfaillie, C M
One of the factors required for successful retroviral transduction is contact between viral particles and target cells. We hypothesized that combining agents that improve virus-target cell...
We evaluated the effect of beta1-integrin receptor engagement on the expression and activity of cell cycle regulatory proteins in CD34(+) cells under conditions that mimic the steady-state marrow...
Oegema, T R, Brazil, J J, Dudek, A Z, Slungaard, A, Verfaillie, C M
We have shown that stromal O-sulfated heparan sulfate glycosaminoglycans (O-S-GAGs) regulate primitive human hematopoietic progenitor cell (HPC) growth and differentiation by colocalizing...
Abnormal circulation and unregulated proliferation of chronic myelogenous leukemia (CML) progenitors is related, at least in part, to BCR/ABL induced abnormalities in beta1 integrin-mediated adhesion...
Gupta, P, Roodell, M, Mortari, F, Verfaillie, C M
Soluble factors produced by human marrow stroma or the murine marrow derived M2-10B4 cell line support ex vivo maintenance for 5-8 weeks of 50% of human long-term culture initiating cells (LTC-IC)....
Wissink, S D, Miller, J S, Moore, K A, Lemischka, I R, Verfaillie, C M
Hematopoietic stem cells (HSC) are cells with self-renewing multilineage differentiation potential. Although engraftment in xenogeneic recipients can be used to measure human HSC, these assays do not...
The mechanism of localization, migration, and regulation of hematopoiesis at different stages of ontogeny is not well understood, but may relate to the specific adhesive interactions between...
Boogaerts, M A, McGlave, P B, Verfaillie, C M
Chronic myelogenous leukemia (CML) is characterized by the Philadelphia (Ph) translocation and BCR/ABL gene rearrangement which occur in a pluripotent hematopoietic progenitor cell. Ph-negative (Ph-)...
Moore, K A, Lemischka, I R, Verfaillie, C M
The goal of this study was to evaluate if differences in culture conditions used in long-term culture assays affect enumeration of LTC-IC in freshly sorted or ex vivo expanded...
Oegema, T R, Brazil, J J, Dudek, A Z, Slungaard, A, Verfaillie, C M
Stem cell localization, conservation, and differentiation is believed to occur in niches in the marrow stromal microenvironment. Our recent observation that long-term in vitro human hematopoiesis...
Abnormal beta1 integrin receptor function may contribute to the continuous proliferation and abnormal circulation of malignant hematopoietic progenitors in chronic myelogenous leukemia (CML)....
We have demonstrated that long-term culture initiating cells (LTC-IC) are maintained in a stroma noncontact (SNC) culture where progenitors are separated from stroma by a microporous membrane and...
Stroncek, D, McCarthy, J B, Verfaillie, C M
Despite the wide use of mobilized peripheral blood (PB) progenitor cells (PBPC) for clinical transplantation the mechanism(s) underlying their mobilization and subsequent engraftment are still...
Blazar, B R, Gupta, K, Verfaillie, C M
Cytokines produced by stromal cells induce the proliferation and differentiation of hematopoietic cells in the marrow microenvironment. We hypothesized that cross-talk between hematopoietic cells at...
Chronic myelogenous leukemia (CML) is characterized by the continuous proliferation and abnormal circulation of malignant hematopoietic progenitors. This may be related to the unresponsiveness of CML...
There is considerable interest in understanding the mechanisms by which interferon-alpha can induce hematological and cytogenetic remissions and prolong survival in patients with chronic myelogenous...
McIvor, R S, Griffin, J D, Verfaillie, C M
Chronic myelogenous leukemia (CML) is a malignant disease of the human hematopoietic stem cell caused by the BCR/ABL gene rearrangement. The only curative therapy is allogeneic transplantation....
Quirk, M R, Kringstad, B A, Verfaillie, C M, Jordan, M C
Murine cytomegalovirus (MCMV) can only be propagated effectively in mouse embryo fibroblast (MEF) cells. We demonstrate that MCMV replicates significantly better in M2-10B4 cells, a continuous line...
McGlave, P B, Miller, J S, Wissink, S, Lin, W N, Verfaillie, C M
FACS-selected CD34+ HLA-DR- cells (DR- cells) may provide a source of benign stem cells suitable for autografting in chronic myelogenous leukemia (CML) and other hematological malignancies. However,...
McCarthy, J B, Kovach, N L, Verfaillie, C M
Adhesion of normal colony-forming cells (CFC) to bone marrow (BM) stroma and the extracellular matrix (ECM) component fibronectin (FN) depends at least in part on the alpha4beta1 and alpha5beta1...
Vanoverbeke, K, Stroncek, D, Verfaillie, C M
We have recently shown that more than 90% of long-term culture initiating cells (LTC-IC) mobilized in the peripheral blood (PB) of normal individuals express HLA-DR and CD38 antigens and can sustain...
We studied the phenotypic and functional properties of colony-forming cells (CFCs), primitive long-term culture initiating cells (LTC-ICs) and lymphoid precursors present in human fetal liver (FL)...
Autologous transplantation therapy for chronic myelogenous leukemia (1997)
Verfaillie, C M, Miller, J S, McGlave, P B
Department of Medicine, University of Minnesota, Minneapolis 55455, USA.
McCarthy, J B, Wayner, E A, Verfaillie, C M
Adhesion receptors can serve as primary signal transduction molecules that convey information into cells that can affect cell proliferation and differentiation. Since hematopoietic progenitors adhere...
Granulocyte colony-stimulating factor (G-CSF) mobilized peripheral blood progenitor cells (PBPC) have successfully been used as stem cells for both autologous and allogeneic transplants. However,...
McCarthy, J B, Verfaillie, C M
Chronic myelogenous leukemia (CML) progenitors show decreased adhesion to stroma and fibronectin (FN) through beta 1 integrin receptors. We have previously shown that interferon-alpha (IFN-alpha)...
McCarthy, J B, Verfaillie, C M
We have recently demonstrated that 50% of primitive human long-term culture-initiating cells (LTC-IC) are maintained for up to 8 weeks in stroma-dependent cultures in which progenitor-stroma contact...
Pierson, B A, McGlave, P B, Verfaillie, C M, Miller, J S
A graft-versus-leukemia effect has been well documented to prevent relapse in chronic myelogenous leukemia (CML) after allogeneic marrow transplantation. One type of lymphocytes that may contribute...
McCarthy, J B, Kovach, N L, Verfaillie, C M
Chronic myelogenous leukemia (CML) is a malignant disease of the hematopoietic stem cell characterized by abnormal circulation and proliferation of malignant progenitors. In contrast to their normal...
A 43-year-old woman developed life-threatening refractory thrombocytopenia following autologous peripheral blood stem cell transplantation for metastatic breast carcinoma. Anti-PLA1 antibodies were...
Autografting for chronic myelogenous leukemia (1995)
Two thirds of patients with chronic myelogenous leukemia do not have suitable donors for allogeneic transplantation. As for other leukemias, autografting may potentially be curative, because normal...
The mechanisms by which interferon-alpha (IFN-alpha) restores normal hematopoiesis in chronic myelogenous leukemia (CML) are not well understood. We have recently demonstrated that IFN-alpha acts...
McCarthy, J B, Verfaillie, C M
In long-term bone marrow cultures, stroma-adherent progenitors proliferate significantly less than nonadherent progenitors. Thus, close progenitor-stroma interactions may serve to regulate or...
McGlave, P B, Dewald, G W, Blazar, B R, Verfaillie, C M
The bone marrow microenvironment supports and regulates the proliferation and differentiation of hematopoietic cells. Dysregulated hematopoiesis in chronic myelogenous leukemia (CML) is caused, at...
Verfaillie, C M, Davies, S M, Filipovich, A H, Wagner, J E, Miller, J S, ...
Delay in hematologic recovery after bone marrow transplantation (BMT) can extend and amplify the risks of infection and hemorrhage, compromise patients' survival, and increase the duration and cost...
Diffusible factors from the murine cell line M2-10B4 support human in vitro hematopoiesis (1994)
Gupta, P, Blazar, B R, Verfaillie, C M
Significantly more primitive human hematopoietic progenitors are maintained and differentiate when cultured separately from the stroma by a microporous membrane ("stroma-noncontact" cultures) than...
Wayner, E A, McGlave, P B, Verfaillie, C M
Treatment of chronic myelogenous leukemia (CML) with interferon-alpha frequently results in normalization of peripheral blood counts and, in up to 20% of patients, reestablishment of normal...
Philadelphia-positive T-acute lymphoblastic leukemia (1985)
Criel, A, Verfaillie, C M, Valcke, Y J, Lamberts, H, Hidajat, M, ...
A case of typical T-acute lymphoblastic leukemia (T-ALL) is reported in which, at diagnosis, 100% of bone marrow metaphases showed a Philadelphia (Ph) translocation, t(9;22). These cells completely...
Hurley, R W, McCarthy, J B, Verfaillie, C M
In long-term bone marrow cultures, stroma-adherent progenitors proliferate significantly less than nonadherent progenitors. Thus, close progenitor-stroma interactions may serve to regulate or...
Bhatia, R, McGlave, P B, Verfaillie, C M
The mechanisms by which interferon-alpha (IFN-alpha) restores normal hematopoiesis in chronic myelogenous leukemia (CML) are not well understood. We have recently demonstrated that IFN-alpha acts...
Bhatia, R, Wayner, E A, McGlave, P B, Verfaillie, C M
Treatment of chronic myelogenous leukemia (CML) with interferon-alpha frequently results in normalization of peripheral blood counts and, in up to 20% of patients, reestablishment of normal...
Verfaillie, C M, McCarthy, J B, McGlave, P B
We studied the adhesion of primitive and committed progenitors from chronic myelogenous leukemia (CML) and normal bone marrow to stroma and to several extracellular matrix components. In contrast to...
Hematopoiesis occurs in different organs during fetal development. Several studies suggest that the growth of hematopoietic progenitors at one stage of ontogenic maturation may not be supported by a...
Prosper, F, Stroncek, D, McCarthy, J B, Verfaillie, C M
Despite the wide use of mobilized peripheral blood (PB) progenitor cells (PBPC) for clinical transplantation the mechanism(s) underlying their mobilization and subsequent engraftment are still...
Hurley, R W, McCarthy, J B, Verfaillie, C M
In long-term bone marrow cultures, stroma-adherent progenitors proliferate significantly less than nonadherent progenitors. Thus, close progenitor-stroma interactions may serve to regulate or...
Bhatia, R, McGlave, P B, Verfaillie, C M
The mechanisms by which interferon-alpha (IFN-alpha) restores normal hematopoiesis in chronic myelogenous leukemia (CML) are not well understood. We have recently demonstrated that IFN-alpha acts...
Bhatia, R, Wayner, E A, McGlave, P B, Verfaillie, C M
Treatment of chronic myelogenous leukemia (CML) with interferon-alpha frequently results in normalization of peripheral blood counts and, in up to 20% of patients, reestablishment of normal...
Verfaillie, C M, McCarthy, J B, McGlave, P B
We studied the adhesion of primitive and committed progenitors from chronic myelogenous leukemia (CML) and normal bone marrow to stroma and to several extracellular matrix components. In contrast to...
Hematopoiesis occurs in different organs during fetal development. Several studies suggest that the growth of hematopoietic progenitors at one stage of ontogenic maturation may not be supported by a...
Prosper, F, Stroncek, D, McCarthy, J B, Verfaillie, C M
Despite the wide use of mobilized peripheral blood (PB) progenitor cells (PBPC) for clinical transplantation the mechanism(s) underlying their mobilization and subsequent engraftment are still...