Martinez, D., Berka, R. M., Henrissat, B., Saloheimo, Markku, Arvas, Mikko, Baker, S. E., ...
Nature Biotechnology Vol.26 Nr.5, 553 - 560
RcoA has pleiotropic effects on Aspergillus nidulans cellular development (2001)
Hicks, J., Lockington, Robin Anthony, Strauss, J., Dieringer, D., Kubicek, C. P., Kelly, Joan Maree, ...
The definitive version is available at www.blackwell-synergy.com
RcoA has pleiotropic effects on Aspergillus nidulans cellular development (2001)
Hicks, J., Lockington, R. A., Strauss, J., Dieringer, D., Kubicek, C. P., Kelly, J. M., ...
RcoA has pleiotropic effects on Aspergillus nidulans cellular development (2001)
Hicks, J., Lockington, Robin Anthony, Strauss, J., Dieringer, D., Kubicek, C. P., Kelly, Joan Maree, ...
The definitive version is available at www.blackwell-synergy.com
Characterization of the pyruvate kinase encoding gene (pki) of Trichoderma reesei. (1993)
Schindler, M., Mach, R.L., Vollenhofer, S.K., Hodits, R., Gruber, F., Visser, J., ...
Regulation of glycolysis by fructose-2,6-biphosphate in A. niger. (1990)
Kubicek, C.P., Harmsen, H., Kubicek-Pranz, E.M., Steinbock, F., Rohr, M., Visser, J.
Regulation of phosphofructokinase 1 and 2 in relation to acidogenesis in Aspergillus niger. (1990)
Steinböck, F., Harmsen, H., Kester, H.C.M., Kubicek-Pranz, E.M., Rohr, M., Visser, J., ...
Kuhls, K, Lieckfeldt, E, Samuels, G J, Kovacs, W, Meyer, W, Petrini, O, ...
The relationship of the important cellulase producing asexual fungus Trichoderma reesei to its putative teleomorphic (sexual) ancestor Hypocrea jecorina and other species of the Trichoderma sect....
In situ detection of protein-DNA interactions in filamentous fungi by in vivo footprinting.
Wolschek, M F, Narendja, F, Karlseder, J, Kubicek, C P, Scazzocchio, C, Strauss, J
The method described here allows the detection of protein-DNA interactions in vivo in filamentous fungi. We outline culture conditions and conditions of in vivo methylation that permit uniform...
Glucose-induced secretion of Trichoderma reesei xylanases.
Kurzatkowski, W, Törrönen, A, Filipek, J, Mach, R L, Herzog, P, Sowka, S, ...
To produce two xylanases with Trichoderma reesei grown on glucose, recombinant strains which carry either the xyn1 or the xyn2 (xylanase I and II [XYN I and XYN II]-encoding) structural genes under...
Kreuzinger, N, Podeu, R, Gruber, F, Göbl, F, Kubicek, C P
Degenerated oligonucleotide primers designed to flank an approximately 1.2-kb fragment of the gene encoding glyceraldehyde-3-phosphate dehydrogenase (gpd) from ascomycetes and basidiomycetes were...
Cell wall synthesis is a major target of mycoparasitic antagonism by Trichoderma harzianum.
Lorito, M, Farkas, V, Rebuffat, S, Bodo, B, Kubicek, C P
We have investigated the molecular basis for the reported synergism between peptaibols and cell wall hydrolytic enzymes in the antagonism of phytopathogenic fungi by Trichoderma harzianum....
Seiboth, B, Hakola, S, Mach, R L, Suominen, P L, Kubicek, C P
The relative contributions of four major cellulases of Trichoderma reesei (1,4-beta-D-glucan cellobiohydrolase I [CBH I], CBH II, endo-1,4-beta-D-glucanase I [EG I], and EG II) to the generation of...
Zeilinger, S, Kristufek, D, Arisan-Atac, I, Hodits, R, Kubicek, C P
Trichoderma reesei RUT C-30 formed an extracellular alpha-galactosidase when it was grown in a batch culture containing lactose or locust bean gum as a carbon source. Short-chain alpha-galactosides...
Schirmböck, M, Lorito, M, Wang, Y L, Hayes, C K, Arisan-Atac, I, Scala, F, ...
Chitinase, beta-1,3-glucanase, and protease activities were formed when Trichoderma harzianum mycelia, grown on glucose as the sole carbon source, were transferred to fresh medium containing cell...
Evidence for a cytoplasmic pathway of oxalate biosynthesis in Aspergillus niger.
Kubicek, C P, Schreferl-Kunar, G, Wöhrer, W, Röhr, M
Oxalate accumulation of up to 8 g/liter was induced in Aspergillus niger by shifting the pH from 6 to 8. This required the presence of Pi and a nitrogen source and was inhibited by the protein...
Schneider, Henry, Lee, Hung, Kubicek, C. P., James, Allen P.
A d-xylose reductase mutant of Pachysolen tannophilus was isolated on the basis of its poor growth on d-xylose but normal growth on xylitol and d-glucose. Fractionation of cell extracts indicated...
Messner, R, Gruber, F, Kubicek, C P
A method consisting of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and subsequent detection of endoglucanases by blotting with a polyclonal antibody against endoglucanase I was used to...
Schreferl, G, Kubicek, C P, Röhr, M
Mutant strains of Aspergillus niger with reduced citrate control of carbohydrate catabolism (cic mutants) grow faster than the parent strain on media containing 5% (wt/vol) citrate. The mutants...
Meixner-Monori, B, Kubicek, C P, Habison, A, Kubicek-Pranz, E M, Röhr, M
alpha-Ketoglutarate dehydrogenase has been demonstrated for the first time in cell extracts from the filamentous fungus Aspergillus niger. A minimum protein concentration of 5 mg/ml is necessary for...
Aconitase and citric acid fermentation by Aspergillus niger.
In view of the often-cited theory that citric acid accumulation is caused by an inhibition of aconitase activity, the equilibrium of the reaction of aconitase was investigated by comparing in vivo...
Subcellular fractionation of cell-free extracts obtained by nitrogen cavitation showed that Penicillium chrysogenum Q176 contains a cytosolic as well as a mitochondrial homocitrate synthase activity....
NADP-specific isocitrate dehydrogenase from the citric acid-accumulating fungus Aspergillus niger.
Meixner-Monori, B, Kubicek, C P, Harrer, W, Schreferl, G, Rohr, M
NADP-specific isocitrate dehydrogenase [threo-DS-isocitrate: NADP+ oxidoreductase (decarboxylating), EC 1.1.1.42] was purified 200-300-fold from the citric acid-accumulating fungus Aspergillus niger....
Partial purification and regulatory properties of phosphofructokinase from Aspergillus niger.
Habison, A, Kubicek, C P, Röhr, M
Phosphofructokinase (EC 2.7.1.11) from a citric acid-producing strain of Aspergillus niger was partially purified by the application of affinity chromatography on Blue Dextran--Sepharose and the use...
The beta-D-xylosidase of Trichoderma reesei is a multifunctional beta-D-xylan xylohydrolase.
Herrmann, M C, Vrsanska, M, Jurickova, M, Hirsch, J, Biely, P, Kubicek, C P
An extracellular multifunctional beta-D-xylan xylohydrolase, previously described as beta-xylosidase, was purified from Trichoderma reesei RUT C-30 to physical homogeneity. The active enzyme was a...
Characterization and properties of protein kinase C from the filamentous fungus Trichoderma reesei.
The Trichoderma reesei pkc1 gene encodes a fungal homologue of the protein kinase C (PKC) family. Using antibodies directed against the nt-sequence-deduced pseudosubstrate domain for identification,...
Kuhls, K, Lieckfeldt, E, Samuels, G J, Kovacs, W, Meyer, W, Petrini, O, ...
The relationship of the important cellulase producing asexual fungus Trichoderma reesei to its putative teleomorphic (sexual) ancestor Hypocrea jecorina and other species of the Trichoderma sect....
In situ detection of protein-DNA interactions in filamentous fungi by in vivo footprinting.
Wolschek, M F, Narendja, F, Karlseder, J, Kubicek, C P, Scazzocchio, C, Strauss, J
The method described here allows the detection of protein-DNA interactions in vivo in filamentous fungi. We outline culture conditions and conditions of in vivo methylation that permit uniform...
Glucose-induced secretion of Trichoderma reesei xylanases.
Kurzatkowski, W, Törrönen, A, Filipek, J, Mach, R L, Herzog, P, Sowka, S, ...
To produce two xylanases with Trichoderma reesei grown on glucose, recombinant strains which carry either the xyn1 or the xyn2 (xylanase I and II [XYN I and XYN II]-encoding) structural genes under...
Kreuzinger, N, Podeu, R, Gruber, F, Göbl, F, Kubicek, C P
Degenerated oligonucleotide primers designed to flank an approximately 1.2-kb fragment of the gene encoding glyceraldehyde-3-phosphate dehydrogenase (gpd) from ascomycetes and basidiomycetes were...
Cell wall synthesis is a major target of mycoparasitic antagonism by Trichoderma harzianum.
Lorito, M, Farkas, V, Rebuffat, S, Bodo, B, Kubicek, C P
We have investigated the molecular basis for the reported synergism between peptaibols and cell wall hydrolytic enzymes in the antagonism of phytopathogenic fungi by Trichoderma harzianum....
Seiboth, B, Hakola, S, Mach, R L, Suominen, P L, Kubicek, C P
The relative contributions of four major cellulases of Trichoderma reesei (1,4-beta-D-glucan cellobiohydrolase I [CBH I], CBH II, endo-1,4-beta-D-glucanase I [EG I], and EG II) to the generation of...
Zeilinger, S, Kristufek, D, Arisan-Atac, I, Hodits, R, Kubicek, C P
Trichoderma reesei RUT C-30 formed an extracellular alpha-galactosidase when it was grown in a batch culture containing lactose or locust bean gum as a carbon source. Short-chain alpha-galactosides...
Schirmböck, M, Lorito, M, Wang, Y L, Hayes, C K, Arisan-Atac, I, Scala, F, ...
Chitinase, beta-1,3-glucanase, and protease activities were formed when Trichoderma harzianum mycelia, grown on glucose as the sole carbon source, were transferred to fresh medium containing cell...
Evidence for a cytoplasmic pathway of oxalate biosynthesis in Aspergillus niger.
Kubicek, C P, Schreferl-Kunar, G, Wöhrer, W, Röhr, M
Oxalate accumulation of up to 8 g/liter was induced in Aspergillus niger by shifting the pH from 6 to 8. This required the presence of Pi and a nitrogen source and was inhibited by the protein...
Schneider, Henry, Lee, Hung, Kubicek, C. P., James, Allen P.
A d-xylose reductase mutant of Pachysolen tannophilus was isolated on the basis of its poor growth on d-xylose but normal growth on xylitol and d-glucose. Fractionation of cell extracts indicated...
Messner, R, Gruber, F, Kubicek, C P
A method consisting of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and subsequent detection of endoglucanases by blotting with a polyclonal antibody against endoglucanase I was used to...
Schreferl, G, Kubicek, C P, Röhr, M
Mutant strains of Aspergillus niger with reduced citrate control of carbohydrate catabolism (cic mutants) grow faster than the parent strain on media containing 5% (wt/vol) citrate. The mutants...
Meixner-Monori, B, Kubicek, C P, Habison, A, Kubicek-Pranz, E M, Röhr, M
alpha-Ketoglutarate dehydrogenase has been demonstrated for the first time in cell extracts from the filamentous fungus Aspergillus niger. A minimum protein concentration of 5 mg/ml is necessary for...
Aconitase and citric acid fermentation by Aspergillus niger.
In view of the often-cited theory that citric acid accumulation is caused by an inhibition of aconitase activity, the equilibrium of the reaction of aconitase was investigated by comparing in vivo...
Subcellular fractionation of cell-free extracts obtained by nitrogen cavitation showed that Penicillium chrysogenum Q176 contains a cytosolic as well as a mitochondrial homocitrate synthase activity....
NADP-specific isocitrate dehydrogenase from the citric acid-accumulating fungus Aspergillus niger.
Meixner-Monori, B, Kubicek, C P, Harrer, W, Schreferl, G, Rohr, M
NADP-specific isocitrate dehydrogenase [threo-DS-isocitrate: NADP+ oxidoreductase (decarboxylating), EC 1.1.1.42] was purified 200-300-fold from the citric acid-accumulating fungus Aspergillus niger....
Partial purification and regulatory properties of phosphofructokinase from Aspergillus niger.
Habison, A, Kubicek, C P, Röhr, M
Phosphofructokinase (EC 2.7.1.11) from a citric acid-producing strain of Aspergillus niger was partially purified by the application of affinity chromatography on Blue Dextran--Sepharose and the use...
The beta-D-xylosidase of Trichoderma reesei is a multifunctional beta-D-xylan xylohydrolase.
Herrmann, M C, Vrsanska, M, Jurickova, M, Hirsch, J, Biely, P, Kubicek, C P
An extracellular multifunctional beta-D-xylan xylohydrolase, previously described as beta-xylosidase, was purified from Trichoderma reesei RUT C-30 to physical homogeneity. The active enzyme was a...
Characterization and properties of protein kinase C from the filamentous fungus Trichoderma reesei.
The Trichoderma reesei pkc1 gene encodes a fungal homologue of the protein kinase C (PKC) family. Using antibodies directed against the nt-sequence-deduced pseudosubstrate domain for identification,...