Expression and purification of a truncated recombinant streptococcal protein G.
Goward, C R, Murphy, J P, Atkinson, T, Barstow, D A
The gene for Protein G from Streptococcus strain G148 was cloned and expressed in Escherichia coli. The regions on the gene corresponding to the albumin-binding domains and the Fab-binding region...
Purification and properties of DNA polymerase from Bacillus caldotenax.
A thermostable DNA polymerase was prepared from Bacillus caldotenax by using a four-step chromatography procedure. The protein exists as a monomer of M(r) 94,000, has a pI of 4.9 and has no...
Goward, C R, Hartwell, R, Atkinson, T, Scawen, M D
Homogeneous glucokinase (EC 2.7.1.2) from the thermophile Bacillus stearothermophilus was isolated on the large scale by using four major steps: precipitation of extraneous material at pH 5.5,...
Goward, C R, Scawen, M D, Atkinson, T
Glucokinase from Bacillus stearothermophilus was irreversibly inactivated by the reactive dichlorotriazinyl dye Procion Blue MX-3G at pH 8.0. The enzyme was protected from inactivation by the...
The secondary structure of protein G', a robust molecule.
Goward, C R, Irons, L I, Murphy, J P, Atkinson, T
The secondary structure of recombinant streptococcal Protein G' was predicted and compared with spectropolarimetric data. The predicted secondary structure consisted of 37 +/- 4% alpha-helix and 30...
Expression and purification of a truncated recombinant streptococcal protein G.
Goward, C R, Murphy, J P, Atkinson, T, Barstow, D A
The gene for Protein G from Streptococcus strain G148 was cloned and expressed in Escherichia coli. The regions on the gene corresponding to the albumin-binding domains and the Fab-binding region...
Purification and properties of DNA polymerase from Bacillus caldotenax.
A thermostable DNA polymerase was prepared from Bacillus caldotenax by using a four-step chromatography procedure. The protein exists as a monomer of M(r) 94,000, has a pI of 4.9 and has no...
Goward, C R, Hartwell, R, Atkinson, T, Scawen, M D
Homogeneous glucokinase (EC 2.7.1.2) from the thermophile Bacillus stearothermophilus was isolated on the large scale by using four major steps: precipitation of extraneous material at pH 5.5,...
Goward, C R, Scawen, M D, Atkinson, T
Glucokinase from Bacillus stearothermophilus was irreversibly inactivated by the reactive dichlorotriazinyl dye Procion Blue MX-3G at pH 8.0. The enzyme was protected from inactivation by the...
The secondary structure of protein G', a robust molecule.
Goward, C R, Irons, L I, Murphy, J P, Atkinson, T
The secondary structure of recombinant streptococcal Protein G' was predicted and compared with spectropolarimetric data. The predicted secondary structure consisted of 37 +/- 4% alpha-helix and 30...
Malate dehydrogenase: a model for structure, evolution, and catalysis.
Goward, C. R., Nicholls, D. J.
Malate dehydrogenases are widely distributed and alignment of the amino acid sequences show that the enzyme has diverged into 2 main phylogenetic groups. Multiple amino acid sequence alignments of...