Fusarium Screen voor tulp: inzicht in de interactie - inzicht in voorkomen (2009)
Breeuwsma, S.J., Duyvesteijn, R.G.E., Jalink, H., Waalwijk, C., ...
Are changes in the composition of the Fusarium Head Blight complex caused by climate change? (2009)
Waalwijk, C., Yang, L., Kema, G.H.J.
Fusarium Head Blight (FHB) of wheat and barley is caused by a complex of species. Apart from yield losses, this disease has attracted much attention due to the capacity of many of the species in the...
Naar een klimaatbestendiger landbouw in Nederland : quickscan en agendering van onderzoek (2009)
Verhagen, A., Blom-Zandstra, M., Kuikman, P.J., Brandenburg, W.A., Elderson, J., ...
Mehrabi, R., Ben M'Barek, S., Waalwijk, C., Kema, G.H.J.
We identified and functionally characterized genes encoding three G proteins and one Gβ protein in the dimorphic fungal wheat pathogen Mycosphaerella graminicola, which we designated MgGpa1, MgGpa2,...
Waalwijk, C., Koch, S.H., Ncube, E., Allwood, J., Flett, B., ...
A quantitative detection tool was developed to enable the monitoring of fumonisin-producing fungi in food and feed commodities. To this end, a quantitative PCR (TaqMan) was developed that targets a...
Fusarium populations on Chinese barley show a dramatic gradient in mycotoxin profiles (2008)
Yang, L.J., Yang, X.J., Yu, D.Z., Waalwijk, C.
We report on a large gene bank of Fusarium isolates established by a broad survey conducted in 2005 in which infected barley ears were collected in 23 counties of seven provinces and two...
Galván, G.A., Koopman, W.J.H., Burger-Meijer, K., Gonzáles, P.H., Waalwijk, C., ...
The aim of this research was to study levels of resistance to Fusarium basal rot in onion cultivars and related Allium species, by using genetically different Fusarium isolates. In order to select...
Rusanova, T., Waalwijk, C., Beekwilder, M.J.
To develop sensor technologies based on genetically engineered recognition elements, recombinant antibodies characterised by high stability are a prerequisite. Here we describe the first successful...
Kema, G.H.J., Mendes, O., Verstappen, E.C.P., Klein Lankhorst, R.M., Sandbrink, H., ...
gus Mycosphaerella graminicola, is currently the most important wheat disease in Europe. Gene expression was examined under highly different conditions, using 10 expressed sequence tag libraries...
Tagging pathogenicity genes in Fusarium graminearum using the transposon system mimp/impala (2008)
Dufresne, M., Ben M'Barek, S., Xu, X., Zhang, X., Liu, T., ...
Transposon mutagenesis was applied to generate Mutants in Fusarium graminearum. The mimp1/impala system originally identified in F. oxysporum proved very promising for mutagenesis as the transposon...
Biodiversity of Fusarium species causing ear rot of maize in Germany (2008)
Görtz, A., Oerke, E.C., Steiner, U., Waalwijk, C., Dehne, H.W.
In Germany, maize is one of the most important agriculture commodities, a major component in animal feed as well as an essential substrate producing biogas. Maize car rot poses a major impact...
Conde, L., Waalwijk, C., Canto-Canché, B.B., Kema, G.H.J., Crous, P.W., James, A.C., ...
Idiomorphs mat1-1 and mat1-2 from Mycosphaerella fijiensis, the causal agent of black leaf streak disease of banana, were isolated. Degenerate oligos were used to amplify the HMG box of the mat1-2...
Keszthelyi, A., Jeney, A., Kerenyi, Z., Mendes, O., Waalwijk, C., Hornok, L.
Mating type in filamentous ascomycetes is controlled by idiomorphic alleles, named MAT1-1 and MAT1-2, which contain 1-3 genes. Of these genes MAT1-1-1 and MAT1-2-1 encode putative transcription...
Ware, S.B., Verstappen, E.C.P., Breeden, J., Cavaletto, J.R., Goodwin, S.B., Waalwijk, C., ...
We studied the possibility of a teleomorph associated with the genotypically diverse septoria speckled leaf blotch (SSLB) pathogen of barley, Septoria passerinii. A teleomorph in the genus...
Molecular diagnostics for the Sigatoka disease complex of banana (2007)
Arzanlou, M., Abeln, E.C.A., Kema, G.H.J., Waalwijk, C., Carlier, J., Crous, P.W.
The Sigatoka disease complex of banana involves three related ascomycetous fungi, Mycosphaerella fijiensis, M. musicola, and M. eumusae. The exact distribution of these three species and their...
Molecular diagnostics for the Sigatoka disease complex of banana (2007)
Arzanlou, M., Abeln, E.C.A., Kema, G.H.J., Waalwijk, C., Carlier, J., ...
The Sigatoka disease complex of banana involves three related ascomycetous fungi, Mycosphaerella fijiensis, M. musicola, and M. eumusae. The exact distribution of these three species and their...
Genetic factors affecting sexual reproduction in toxigenic Fusarium species (2007)
Hornok, L., Waalwijk, C., Leslie, J.F.
Mycotoxin producing capability greatly varies within species. In theory, the major source of this variability is meiotic recombination. However, a number of important toxigenic species have no known...
Köhl, J., Kastelein, P., Waalwijk, C.
Naturally occurring populations of Fusarium avenaceum, F. culmorum, F. graminearum, F. poae, and Microdochium nivale were studied in two field experiments from anthesis in June 2003 until harvest in...
Mehrabi, R., Waalwijk, C., Kema, G.H.J.
Among expressed sequence tag libraries of Mycosphaerella graminicola isolate IPO323, we identified a full-length cDNA clone with high homology to the mitogen-activated protein (MAP) kinase Slt2 in...
Cousin, A., Mehrabi, R., Guilleroux, M., Dufresne, M., Waalwijk, C., ...
In eukaryotes, a family of serine/threonine protein kinases known as mitogen-activated protein kinases (MAPKs) is involved in the transduction of a variety of extracellular signals and in the...
Identification of the Teleomorph of Septoria Passerinii, the Barley speckled leaf pathogen (2006)
Ware, S.B., Verstappen, E.C.P., Cavaletto, J.R., Goodwin, S.B., Waalwijk, C., Kema, G.H.J.
Jalink, H., Waalwijk, C., Kema, G.H.J.
A complex of Fusarium spp. causes head blight on wheat. Unlike the advances in the area of Fusarium genomics, progress in the understanding of the infection process and the possible resistance...
Ware, S.B., Verstappen, E.C.P., Breeden, J., Cavaletto, J.R., Waalwijk, C., Crous, P.W., ...
We studied the possibility of a teleomorph associated with the genotypically diverse septoria speckled leaf blotch (SSLB) pathogen of barley, Septoria passerinii. A teleomorph in the genus...
Hagenaars, T.J., Elbers, A.R.W., Kleter, G.A., Kreft, F., Waalwijk, C., ...
In this report six case studies are carried out on food-safety and animal-health crises that have occurred in the recent past. The aim is to learn from these cases if and how the identification of...
Advances in molecular diagnosis of toxigenic Fusarium species (2005)
Mulé, G., Gonzalez-Jaen, M.T., Hornok, L., Nicholson, P., Waalwijk, C.
The development of advanced molecular diagnosis for the critical toxigenic Fusarium species is considered in this review. The specific topics discussed are (1) isolation of mating type genes of...
Jalink, H., Kema, G.H.J., Waalwijk, C.
A complex of Fusarium spp. causes head blight on wheat. Unlike the advances in the area of Fusarium genomics, progress in the understanding of the infection process and the possible resistance...
Quantitative detection of Fusarium species in wheat using TaqMan (2004)
Waalwijk, C., Schoen, C.D., Costrel-Decorainville, G., ...
Fusarium head blight (FHB) of wheat and other small-grain cereals is a disease complex caused by several fungal species. To monitor and quantify the major species in the FHB complex during the...
From single to multiplex detection of plant pathogens by micro-arrays (2004)
Bonants, P.J.M., Schoen, C.D., Szemes, M., Klerks, M.M., ...
Mating type sequences in asexually reproducing Fusarium species (2004)
Kenényi, Z., Moretti, A., Waalwijk, C., Oláh, B., Hornok, L.
To assess the potential for mating in several Fusarium species with no known sexual stage, we developed degenerate and semidegenerate oligonucleotide primers to identify conserved mating type (MAT)...
Gene huning in Mycospaerella graminicola (2004)
Waalwijk, C., Verstappen, E.C.P., Mendes, O., Klein Lankhorst, R.M., Sandbrink, H., ...
Waalwijk, C., Hesselink, T., Arts, J., Kema, G.H.J.
A comparative genomic approach was used to study the mating type locus and the gene cluster involved in toxin production (fumonisin) in Fusarium proliferatum, a pathogen with a wide host range and a...
Detection of Fusarium in single wheat kernels using spectral imaging (2004)
G. Polder, C. Waalwijk, I. T. Young
Fusarium head blight (FHB) is a harmful fungal disease that occurs in small grains. Non-destructive detection of this disease is traditionally done using spectroscopy or image processing. In this...
Gene hunting in Mycosphaerella graminicola (2003)
Kema, G.H.J., Verstappen, E.C.P., Mendes, O., Sandbrink, H., Klein Lankhorst, R.M., ...
Major changes in Fusarium spp. in wheat in The Netherlands (2003)
Waalwijk, C., Kastelein, P., Kerényi, Z., Hesselink, T., Arts, J., ...
The re-emergence of fusarium head blight throughout the world and especially in Western Europe prompted a survey of the situation in the Netherlands. To allow for a high throughput screening of large...
Goodwin, S.B., Cavaletto, J.R., Zhang, G., Waalwijk, C., Kema, G.H.J.
The genus Septoria contains more than 1000 species of plant pathogenic fungi, most of which have no known sexual stage. Species of Septoria without a known sexual stage could be recent derivatives of...
Major changes in Fusarium spp. in wheat in The Netherlands (2003)
Waalwijk, C., Kastelein, P., Kerényi, Z., Hesselink, T., ...
The re-emergence of fusarium head blight throughout the world and especially in Western Europe prompted a survey of the situation in the Netherlands. To allow for a high throughput screening of large...
Experimental identification of the teleomorph of the barley pathogen Septoria passerinii (2003)
Ware, S.B., Verstappen, E.C.P., Goodwin, S.B., Waalwijk, C., Kema, G.H.J.
Waalwijk, C., Mendes, O., Verstappen, E.C.P., Kema, G.H.J.
Both mating-type loci from the wheat septoria leaf blotch pathogen Mycosphaerella graminicola have been cloned and sequenced. The MAT1-2 gene was identified by screening a genomic library from the...
Zhan, J., Kema, G.H.J., Waalwijk, C., McDonald, B.A.
A total of 2035 Mycosphaerella graminicola strains collected from 16 geographic locations on four continents were assayed for the mating type locus. RFLP fingerprints were used to identify clones in...
Molecular relationships of fungi within the Fusarium redolens - F. hostae clade (2001)
Baayen, R.P., O'Donnell, K., Breeuwsma, S., Geiser, D.M., Waalwijk, C.
The evolutionary relationships of fungi in the Fusarium redolens - F. hostae clade were investigated by constructing nuclear and mitochondrial gene genealogies for 37 isolates representing the known...
Goodwin, S.B., Cavaletto, J.R., Waalwijk, C., Kema, G.H.J.
DNA fingerprinting has been used extensively to characterize populations of Mycosphaerella graminicola, the Septoria tritici blotch pathogen of wheat. The highly polymorphic DNA fingerprints of...
Fusarium redolens f.sp. asparagi, causal agent of asparagus root rot, crown rot and spear rot (2000)
Baayen, R.P., Bonants, P.J.M., Poll, J.T.K., Blok, W.J., Waalwijk, C.
Mapping the Mycosphaerella graminicola genome (1999)
Kema, G.H.J., Goodwin, S.B., Hamza, S., Verstappen, E.C.P., Cavaletto, J.R., ...
Phylogeny of VCGs and formae speciales of Fusarium oxysporum and F. redolens (1999)
Baayen, R.P., O'Donnell, K., Waalwijk, C., Bonants, P.J.M., Cigelnik, E., Roebroeck, E.J.A.
Een koppelingskaart van het Mycosphaerella graminicola [anamorph Septoria tritici] genoom. (1998)
Kema, G.H.J., Hamza, S., Bonants, P.J.M., Waalwijk, C., Verstappen, E.C.P., ...
Genetic diversity in Fusarium oxysporum f.sp. dianthi and F. redolens f.sp. dianthi (1997)
Baayen, R.P., Krijger, M.C., Waalwijk, C.
Pathogenic isolates were selected representing all known vegetative compatibility groups (VCGs) and races of Fusarium oxysporum sensu lato from Dianthus spp. On basis of differences in the internal...
Functional Identification of Plant Pathogenic Fungi: concerted action (1996)
Keijer, J., Waalwijk, C., Kema, G., Bonants, P., Dullemans, A.M., Patino, C., ...
Functional identification of plant pathogenic fungi (1995)
Keijer, J., Waalwijk, C., Kema, G., Bonants, P., Dullemans, A.M., Houterman, P.M., ...
We have analysed DNA modification in a HapII site (CCGG) present in the major intron of the discontinuous rabbit β-globin gene. In most somatic tissues, including erythroid and non-erythroid...
MspI, an isoschizomer of HpaII which cleaves both unmethylated and methylated HpaII sites (1978)
The cleavage of DNA by restriction endonucleases HpaII and HapII is prevented by the presence of a 5-methyl group at the internal C residue of its recognition sequence CCGG. MspI, an isoschizomer of...
Waalwijk, C, De Graaff, J, MacLaren, D M
The hemolysin plasmid pCW2, which enhances the virulence of Escherichia coli, has been mapped. Comparison of the region coding for hemolysin with other hemolysin determinants reveals differences that...
In vivo function of hemolysin in the nephropathogenicity of Escherichia coli.
Waalwijk, C, MacLaren, D M, De Graaff, J
The role of hemolysin in the nephropathogenicity of Escherichia coli was studied in a hematogenous pyelonephritis model in mice. The nephropathogenicity of a nonhemolytic, avirulent E. coli strain...
Waalwijk, C, Dullemans, A M, Van Workum, M E, Visser, B
The Mr 28.000 crystal protein gene of Bacillus thuringiensis subspecies israelensis has been cloned into pBR322 as part of a 9.7 kb HindIII fragment. From hybridization experiments of recombinant...
MspI, an isoschizomer of hpaII which cleaves both unmethylated and methylated hpaII sites.
The cleavage of DNA by restriction endonucleases HpaII and HapII is prevented by the presence of a 5-methyl group at the internal C residue of its recognition sequence CCGG. MspI, an isoschizomer of...
We have analysed DNA modification in a HapII site (CCGG) present in the major intron of the discontinuous rabbit β-globin gene. In most somatic tissues, including erythroid and non-erythroid...
Hemolysin plasmid coding for the virulence of a nephropathogenic Escherichia coli strain.
Waalwijk, C, Van Den Bosch, J F, MacLaren, D M, De Graaff, J
The nephropathogenic Escherichia coli strain P673 was shown to harbor two plasmids with molecular sizes of 70 and 41 megadaltons, respectively. The 70-megadalton plasmid, pCW1, coded for tetracycline...
Feldmann, F., Dullemans, A., Waalwijk, C.
Ligand-blotting experiments on dipteran brush border membrane vesicles (BBMVs) showed binding of CryIVD toxin of Bacillus thuringiensis subsp. israelensis to proteins of 148 kDa in Anopheles...
Waalwijk, C, De Graaff, J, MacLaren, D M
The hemolysin plasmid pCW2, which enhances the virulence of Escherichia coli, has been mapped. Comparison of the region coding for hemolysin with other hemolysin determinants reveals differences that...
In vivo function of hemolysin in the nephropathogenicity of Escherichia coli.
Waalwijk, C, MacLaren, D M, De Graaff, J
The role of hemolysin in the nephropathogenicity of Escherichia coli was studied in a hematogenous pyelonephritis model in mice. The nephropathogenicity of a nonhemolytic, avirulent E. coli strain...
Waalwijk, C, Dullemans, A M, Van Workum, M E, Visser, B
The Mr 28.000 crystal protein gene of Bacillus thuringiensis subspecies israelensis has been cloned into pBR322 as part of a 9.7 kb HindIII fragment. From hybridization experiments of recombinant...
MspI, an isoschizomer of hpaII which cleaves both unmethylated and methylated hpaII sites.
The cleavage of DNA by restriction endonucleases HpaII and HapII is prevented by the presence of a 5-methyl group at the internal C residue of its recognition sequence CCGG. MspI, an isoschizomer of...
We have analysed DNA modification in a HapII site (CCGG) present in the major intron of the discontinuous rabbit β-globin gene. In most somatic tissues, including erythroid and non-erythroid...
Hemolysin plasmid coding for the virulence of a nephropathogenic Escherichia coli strain.
Waalwijk, C, Van Den Bosch, J F, MacLaren, D M, De Graaff, J
The nephropathogenic Escherichia coli strain P673 was shown to harbor two plasmids with molecular sizes of 70 and 41 megadaltons, respectively. The 70-megadalton plasmid, pCW1, coded for tetracycline...
Feldmann, F., Dullemans, A., Waalwijk, C.
Ligand-blotting experiments on dipteran brush border membrane vesicles (BBMVs) showed binding of CryIVD toxin of Bacillus thuringiensis subsp. israelensis to proteins of 148 kDa in Anopheles...