Ozimek, Paulina, Dijk, Ralf Van, Latchev, Kantcho, Gancedo, Carlos, Wang, Dong Yuan, Klei, Ida J. Van Der, ...
Hansenula polymorpha ass3 mutants are characterized by the accumulation of inactive alcohol oxidase (AO) monomers in the cytosol, whereas other peroxisomal matrix proteins are normally activated and...
The lactate-proton symport of Saccharomyces cerevisiae is encoded by JEN1 (1999)
Casal, Margarida, Paiva, Sandra, Andrade, Raquel P., Gancedo, Carlos, Leão, Cecília
A mutant of Saccharomyces cerevisiae deficient in the lactate-proton symport was isolated. Transformation of the mutant with a yeast genomic library allowed the isolation of the gene JEN1 that...
In situ study of the glycolytic pathway in Saccharomyces cerevisiae (1978)
Gancedo, Carlos, Bañuelos, Marcelino
1. The problem of the influence of protein concentration on the kinetic parameters of enzymes has been approached studying the glycolytic enzymes from Saccharomyces cerevisiae in permeabilized cells...
The Lactate-Proton Symport of Saccharomyces cerevisiae Is Encoded by JEN1
Casal, Margarida, Paiva, Sandra, Andrade, Raquel P., Gancedo, Carlos, Leão, Cecília
A mutant of Saccharomyces cerevisiae deficient in the lactate-proton symport was isolated. Transformation of the mutant with a yeast genomic library allowed the isolation of the gene JEN1 that...
Zaragoza, Oscar, Rodríguez, Cristina, Gancedo, Carlos
We have cloned a Candida albicans gene (CaMIG1) that encodes a protein homologous to the DNA-binding protein Mig1 from Saccharomyces cerevisiae (ScMig1). The C. albicans Mig1 protein (CaMig1) differs...
Zaragoza, Oscar, Blazquez, Miguel A., Gancedo, Carlos
The TPS1 gene from Candida albicans, which encodes trehalose-6-phosphate synthase, has been cloned by functional complementation of a tps1 mutant from Saccharomyces cerevisiae. In contrast with the...
Petit, Thomas, Diderich, Jasper A., Kruckeberg, Arthur L., Gancedo, Carlos, Van Dam, Karel
Glucose transport kinetics and mRNA levels of different glucose transporters were determined in Saccharomyces cerevisiae strains expressing different sugar kinases. During exponential growth on...
Ozimek, Paulina, Van Dijk, Ralf, Latchev, Kantcho, Gancedo, Carlos, Wang, Dong Yuan, Van Der Klei, Ida J., ...
Hansenula polymorpha ass3 mutants are characterized by the accumulation of inactive alcohol oxidase (AO) monomers in the cytosol, whereas other peroxisomal matrix proteins are normally activated and...
Inactivation of 1,6-Diphosphatase by Glucose in Yeast
Fructose-1,6-diphosphatase was derepressed in Saccharomyces cerevisiae by incubation in media containing non-sugar carbon sources. Addition of glucose to a derepressed culture led to a rapid loss of...
Peña-Diaz, Javier, Montalvetti, Andrea, Flores, Carmen-Lisset, Constán, Aurora, Hurtado-Guerrero, Ramon, De Souza, Wanderley, ...
3-Hydroxy-3-methyl-glutaryl-CoA reductase (HMGR) is a key enzyme in the sterol biosynthesis pathway, but its subcellular distribution in the Trypanosomatidae family is somewhat controversial....
Flores, Carmen-Lisset, Gancedo, Carlos
We have cloned and characterized the gene PYC1, encoding the unique pyruvate carboxylase in the dimorphic yeast Yarrowia lipolytica. The protein putatively encoded by the cDNA has a length of 1,192...
The Lactate-Proton Symport of Saccharomyces cerevisiae Is Encoded by JEN1
Casal, Margarida, Paiva, Sandra, Andrade, Raquel P., Gancedo, Carlos, Leão, Cecília
A mutant of Saccharomyces cerevisiae deficient in the lactate-proton symport was isolated. Transformation of the mutant with a yeast genomic library allowed the isolation of the gene JEN1 that...
Zaragoza, Oscar, Rodríguez, Cristina, Gancedo, Carlos
We have cloned a Candida albicans gene (CaMIG1) that encodes a protein homologous to the DNA-binding protein Mig1 from Saccharomyces cerevisiae (ScMig1). The C. albicans Mig1 protein (CaMig1) differs...
Zaragoza, Oscar, Blazquez, Miguel A., Gancedo, Carlos
The TPS1 gene from Candida albicans, which encodes trehalose-6-phosphate synthase, has been cloned by functional complementation of a tps1 mutant from Saccharomyces cerevisiae. In contrast with the...
Petit, Thomas, Diderich, Jasper A., Kruckeberg, Arthur L., Gancedo, Carlos, Van Dam, Karel
Glucose transport kinetics and mRNA levels of different glucose transporters were determined in Saccharomyces cerevisiae strains expressing different sugar kinases. During exponential growth on...
Ozimek, Paulina, Van Dijk, Ralf, Latchev, Kantcho, Gancedo, Carlos, Wang, Dong Yuan, Van Der Klei, Ida J., ...
Hansenula polymorpha ass3 mutants are characterized by the accumulation of inactive alcohol oxidase (AO) monomers in the cytosol, whereas other peroxisomal matrix proteins are normally activated and...
Inactivation of 1,6-Diphosphatase by Glucose in Yeast
Fructose-1,6-diphosphatase was derepressed in Saccharomyces cerevisiae by incubation in media containing non-sugar carbon sources. Addition of glucose to a derepressed culture led to a rapid loss of...
Peña-Diaz, Javier, Montalvetti, Andrea, Flores, Carmen-Lisset, Constán, Aurora, Hurtado-Guerrero, Ramon, De Souza, Wanderley, ...
3-Hydroxy-3-methyl-glutaryl-CoA reductase (HMGR) is a key enzyme in the sterol biosynthesis pathway, but its subcellular distribution in the Trypanosomatidae family is somewhat controversial....
Flores, Carmen-Lisset, Gancedo, Carlos
We have cloned and characterized the gene PYC1, encoding the unique pyruvate carboxylase in the dimorphic yeast Yarrowia lipolytica. The protein putatively encoded by the cDNA has a length of 1,192...
Moonlighting Proteins in Yeasts
Gancedo, Carlos, Flores, Carmen-Lisset
Proteins able to participate in unrelated biological processes have been grouped under the generic name of moonlighting proteins. Work with different yeast species has uncovered a great number of...
Jardón, Raquel, Gancedo, Carlos, Flores, Carmen-Lisset
The genes encoding gluconeogenic enzymes in the nonconventional yeast Yarrowia lipolytica were found to be differentially regulated. The expression of Y. lipolytica FBP1 (YlFBP1) encoding the key...