Carmelo Di Primo

A global benchmark study using affinity-based biosensors (2009)

Rich, Rebecca L., Papalia, Giuseppe A., Flynn, Peter J., Furneisen, Jamie, Quinn, John, Klein, Joshua S., ...

To explore the variability in biosensor studies, 150 participants from 20 countries were given the same protein samples and asked to determine kinetic rate constants for the interaction. We chose a...

Real time analysis of the RNAI-RNAII-Rop complex by surface plasmon resonance: from a decaying surface to a standard kinetic analysis. (2008)

Di Primo, Carmelo

RNA loop-loop complexes are motifs that regulate biological functions in both prokaryotic and eukaryotic organisms. In E. coli, RNAI, an antisense RNA encoded by the ColE1 plasmid, regulates the...

Real time analysis of the RNAI-RNAII-Rop complex by surface plasmon resonance: from a decaying surface to a standard kinetic analysis. (2008)

Di Primo, Carmelo

RNA loop-loop complexes are motifs that regulate biological functions in both prokaryotic and eukaryotic organisms. In E. coli, RNAI, an antisense RNA encoded by the ColE1 plasmid, regulates the...

Real time analysis of the RNAI-RNAII-Rop complex by surface plasmon resonance: from a decaying surface to a standard kinetic analysis. (2008)

Di Primo, Carmelo

RNA loop-loop complexes are motifs that regulate biological functions in both prokaryotic and eukaryotic organisms. In E. coli, RNAI, an antisense RNA encoded by the ColE1 plasmid, regulates the...

Exploring TAR-RNA aptamer loop-loop interaction by X-ray crystallography, UV spectroscopy and surface plasmon resonance. (2008)

Lebars, Isabelle, Legrand, Pierre, Aimé, Ahissan, Pinaud, Noël, Fribourg, Sébastien, Di Primo, Carmelo

In HIV-1, trans-activation of transcription of the viral genome is regulated by an imperfect hairpin, the trans-activating responsive (TAR) RNA element, located at the 5' untranslated end of all...

Exploring TAR-RNA aptamer loop-loop interaction by X-ray crystallography, UV spectroscopy and surface plasmon resonance. (2008)

Lebars, Isabelle, Legrand, Pierre, Aimé, Ahissan, Pinaud, Noël, Fribourg, Sébastien, Di Primo, Carmelo

In HIV-1, trans-activation of transcription of the viral genome is regulated by an imperfect hairpin, the trans-activating responsive (TAR) RNA element, located at the 5' untranslated end of all...

Exploring TAR-RNA aptamer loop-loop interaction by X-ray crystallography, UV spectroscopy and surface plasmon resonance. (2008)

Lebars, Isabelle, Legrand, Pierre, Aimé, Ahissan, Pinaud, Noël, Fribourg, Sébastien, Di Primo, Carmelo

In HIV-1, trans-activation of transcription of the viral genome is regulated by an imperfect hairpin, the trans-activating responsive (TAR) RNA element, located at the 5' untranslated end of all...

NMR structure of a kissing complex formed between the TAR RNA element of HIV-1 and a LNA-modified aptamer (2007)

Lebars, Isabelle, Richard, Tristan, Di Primo, Carmelo, Toulmé, Jean-Jacques

The trans-activating responsive (TAR) RNA element located in the 5′ untranslated region of the HIV-1 genome is a 57-nt imperfect stem-loop essential for the viral replication. TAR regulates...

Molecular dynamics reveals the stabilizing role of loop closing residues in kissing interactions: comparison between TAR-TAR* and TAR-aptamer (2003)

Beaurain, François, Di Primo, Carmelo, Toulmé, Jean Jacques, Laguerre, Michel

A RNA aptamer (R06) raised against the trans‐ activation responsive (TAR) element of HIV‐1 was previously shown to generate a loop–loop complex whose stability is strongly dependent on...

Loop–loop interaction of HIV-1 TAR RNA with N3′ → P5′ deoxyphosphoramidate aptamers inhibits in vitro Tat-mediated transcription

Darfeuille, Fabien, Arzumanov, Andrey, Gryaznov, Sergei, Gait, Michael J., Di Primo, Carmelo, Toulmé, Jean-Jacques

A hairpin RNA aptamer has been identified by in vitro selection against the transactivation-responsive element (TAR) of HIV-1. A nuclease-resistant N3′ → P5′ phosphoramidate isosequential...

Molecular dynamics reveals the stabilizing role of loop closing residues in kissing interactions: comparison between TAR–TAR* and TAR–aptamer

Beaurain, François, Di Primo, Carmelo, Toulmé, Jean Jacques, Laguerre, Michel

A RNA aptamer (R06) raised against the trans- activation responsive (TAR) element of HIV-1 was previously shown to generate a loop–loop complex whose stability is strongly dependent on the selected...

LNA/DNA chimeric oligomers mimic RNA aptamers targeted to the TAR RNA element of HIV-1

Darfeuille, Fabien, Bo Hansen, Jens, Orum, Henrik, Di Primo, Carmelo, Toulmé, Jean-Jacques

One of the major limitations of the use of phosphodiester oligonucleotides in cells is their rapid degradation by nucleases. To date, several chemical modifications have been employed to overcome...

Loop–loop interaction of HIV-1 TAR RNA with N3′ → P5′ deoxyphosphoramidate aptamers inhibits in vitro Tat-mediated transcription

Darfeuille, Fabien, Arzumanov, Andrey, Gryaznov, Sergei, Gait, Michael J., Di Primo, Carmelo, Toulmé, Jean-Jacques

A hairpin RNA aptamer has been identified by in vitro selection against the transactivation-responsive element (TAR) of HIV-1. A nuclease-resistant N3′ → P5′ phosphoramidate isosequential...

Molecular dynamics reveals the stabilizing role of loop closing residues in kissing interactions: comparison between TAR–TAR* and TAR–aptamer

Beaurain, François, Di Primo, Carmelo, Toulmé, Jean Jacques, Laguerre, Michel

A RNA aptamer (R06) raised against the trans- activation responsive (TAR) element of HIV-1 was previously shown to generate a loop–loop complex whose stability is strongly dependent on the selected...

LNA/DNA chimeric oligomers mimic RNA aptamers targeted to the TAR RNA element of HIV-1

Darfeuille, Fabien, Bo Hansen, Jens, Orum, Henrik, Di Primo, Carmelo, Toulmé, Jean-Jacques

One of the major limitations of the use of phosphodiester oligonucleotides in cells is their rapid degradation by nucleases. To date, several chemical modifications have been employed to overcome...

NMR structure of a kissing complex formed between the TAR RNA element of HIV-1 and a LNA-modified aptamer

Lebars, Isabelle, Richard, Tristan, Di Primo, Carmelo, Toulmé, Jean-Jacques

The trans-activating responsive (TAR) RNA element located in the 5′ untranslated region of the HIV-1 genome is a 57-nt imperfect stem-loop essential for the viral replication. TAR regulates...

Liquid-crystal NMR structure of HIV TAR RNA bound to its SELEX RNA aptamer reveals the origins of the high stability of the complex

Van Melckebeke, Hélène, Devany, Matthew, Di Primo, Carmelo, Beaurain, François, Toulmé, Jean-Jacques, Bryce, David L., ...

Transactivation-response element (TAR) is a stable stem–loop structure of HIV RNA, which plays a crucial role during the life cycle of the virus. The apical loop of TAR acts as a binding site for...

Exploring TAR–RNA aptamer loop–loop interaction by X-ray crystallography, UV spectroscopy and surface plasmon resonance

Lebars, Isabelle, Legrand, Pierre, Aimé, Ahissan, Pinaud, Noël, Fribourg, Sébastien, Di Primo, Carmelo

In HIV-1, trans-activation of transcription of the viral genome is regulated by an imperfect hairpin, the trans-activating responsive (TAR) RNA element, located at the 5′ untranslated end of all...