Carol D. Blair

Potential for La Crosse virus segment reassortment in nature (2008)

Reese, Sara M, Blitvich, Bradley J, Blair, Carol D, Geske, Dave, Beaty, Barry J, Black, William C

Abstract The evolutionary success of La Crosse virus (LACV, family Bunyaviridae ) is due to its ability to adapt to changing conditions through intramolecular genetic changes and segment...

Aedes aegyptiuses RNA interference in defense against Sindbis virus infection (2008)

Campbell, Corey L, Keene, Kimberly M, Brackney, Douglas E, Olson, Ken E, Blair, Carol D, Wilusz, Jeffrey, ...

Abstract Background RNA interference (RNAi) is an important anti-viral defense mechanism. The Aedes aegypti genome encodes RNAi component orthologs, however, most populations of this mosquito are...

Development and Application of Nucleic Acid Hybridization Techniques to Arbovirus Surveillance and Diagnosis. (1999)

Beaty, Barry J., Blair, Carol D.

Nucleic acid hybridization techniques have been developed to detect dengue and LaCrosse arbovirus RNA in cells and in cell and tissue suspensions. Probes are comprised of cDNAs of portions of the...

Development and Application of Nucleic Acid Hybridization Techniques to Arbovirus Surveillance and Diagnosis. (1998)

Beaty,Barry J., Blair,Carol D.

Sandwich hybridization techniques have been developed for detection of dengue virus RNA. Capture and detector probe constructs have been prepared in M13 and in vitro transcription plasmids,...

Development and Application of Nucleic Acid Hybridization Techniques to Arbovirus Surveillance and Diagnosis. (1998)

Beaty, Barry J., Blair, Carol D.

Nucleic acid hybridization techniques have been developed for detection of LaCrosse and dengue virus RNA in cells and in mosquitoes. RNA transcript or DNA probes of the respective viruses were...

Molecular Strategies for Interrupting Arthropod-Borne Virus Transmission by Mosquitoes

Blair, Carol D., Adelman, Zachary N., Olson, Ken E.

Arthropod-borne virus (arbovirus) infections cause a number of emerging and resurgent human and veterinary infectious diseases. Traditional means of controlling arbovirus diseases include vaccination...

RNA Silencing of Dengue Virus Type 2 Replication in Transformed C6/36 Mosquito Cells Transcribing an Inverted-Repeat RNA Derived from the Virus Genome

Adelman, Zach N., Sanchez-Vargas, Irma, Travanty, Emily A., Carlson, Jon O., Beaty, Barry J., Blair, Carol D., ...

Double-stranded RNA (dsRNA) initiates cellular posttranscriptional responses that are collectively called RNA silencing in a number of different organisms, including plants, nematodes, and fruit...

Effect of Sendai Virus Infection on Lipid Metabolism in Chick Embryo Fibroblasts

Blair, Carol D., Brennan, Patrick J.

Lipid metabolism in the “abortive” system, Sendai virus-infected primary chick embryo fibroblasts, was examined by using 32P-orthophosphate, 14C-glucose, and 14C-glycerol as precursors....

Replication of Sendai Virus: II. Steps in Virus Assembly

Blair, Carol D., Robinson, William S.

Chick embryo fibroblast cultures infected with Sendai virus were incubated with 3H-uridine in the presence of actinomycin D beginning at 18 hr after infection. The 35 and 18S virus-specific...

RNA interference acts as a natural antiviral response to O'nyong-nyong virus (Alphavirus; Togaviridae) infection of Anopheles gambiae

Keene, Kimberly M., Foy, Brian D., Sanchez-Vargas, Irma, Beaty, Barry J., Blair, Carol D., Olson, Ken E.

RNA interference (RNAi) is triggered in eukaryotic organisms by double-stranded RNA (dsRNA), and it destroys any mRNA that has sequence identity with the dsRNA trigger. The RNAi pathway in Anopheles...

Engineering RNA interference-based resistance to dengue virus type 2 in genetically modified Aedes aegypti

Franz, Alexander W. E., Sanchez-Vargas, Irma, Adelman, Zach N., Blair, Carol D., Beaty, Barry J., James, Anthony A., ...

Mosquitoes (Aedes aegypti) were genetically modified to exhibit impaired vector competence for dengue type 2 viruses (DENV-2). We exploited the natural antiviral RNA interference (RNAi) pathway in...

Molecular Strategies for Interrupting Arthropod-Borne Virus Transmission by Mosquitoes

Blair, Carol D., Adelman, Zachary N., Olson, Ken E.

Arthropod-borne virus (arbovirus) infections cause a number of emerging and resurgent human and veterinary infectious diseases. Traditional means of controlling arbovirus diseases include vaccination...

RNA Silencing of Dengue Virus Type 2 Replication in Transformed C6/36 Mosquito Cells Transcribing an Inverted-Repeat RNA Derived from the Virus Genome

Adelman, Zach N., Sanchez-Vargas, Irma, Travanty, Emily A., Carlson, Jon O., Beaty, Barry J., Blair, Carol D., ...

Double-stranded RNA (dsRNA) initiates cellular posttranscriptional responses that are collectively called RNA silencing in a number of different organisms, including plants, nematodes, and fruit...

Effect of Sendai Virus Infection on Lipid Metabolism in Chick Embryo Fibroblasts

Blair, Carol D., Brennan, Patrick J.

Lipid metabolism in the “abortive” system, Sendai virus-infected primary chick embryo fibroblasts, was examined by using 32P-orthophosphate, 14C-glucose, and 14C-glycerol as precursors....

Replication of Sendai Virus: II. Steps in Virus Assembly

Blair, Carol D., Robinson, William S.

Chick embryo fibroblast cultures infected with Sendai virus were incubated with 3H-uridine in the presence of actinomycin D beginning at 18 hr after infection. The 35 and 18S virus-specific...

RNA interference acts as a natural antiviral response to O'nyong-nyong virus (Alphavirus; Togaviridae) infection of Anopheles gambiae

Keene, Kimberly M., Foy, Brian D., Sanchez-Vargas, Irma, Beaty, Barry J., Blair, Carol D., Olson, Ken E.

RNA interference (RNAi) is triggered in eukaryotic organisms by double-stranded RNA (dsRNA), and it destroys any mRNA that has sequence identity with the dsRNA trigger. The RNAi pathway in Anopheles...

Engineering RNA interference-based resistance to dengue virus type 2 in genetically modified Aedes aegypti

Franz, Alexander W. E., Sanchez-Vargas, Irma, Adelman, Zach N., Blair, Carol D., Beaty, Barry J., James, Anthony A., ...

Mosquitoes (Aedes aegypti) were genetically modified to exhibit impaired vector competence for dengue type 2 viruses (DENV-2). We exploited the natural antiviral RNA interference (RNAi) pathway in...

La Crosse Bunyavirus Nonstructural Protein NSs Serves To Suppress the Type I Interferon System of Mammalian Hosts▿

Blakqori, Gjon, Delhaye, Sophie, Habjan, Matthias, Blair, Carol D., Sánchez-Vargas, Irma, Olson, Ken E., ...

La Crosse virus (LACV) is a mosquito-transmitted member of the Bunyaviridae family that causes severe encephalitis in children. For the LACV nonstructural protein NSs, previous overexpression studies...

Potential for La Crosse virus segment reassortment in nature

Reese, Sara M, Blitvich, Bradley J, Blair, Carol D, Geske, Dave, Beaty, Barry J, Black, William C

The evolutionary success of La Crosse virus (LACV, family Bunyaviridae) is due to its ability to adapt to changing conditions through intramolecular genetic changes and segment reassortment. Vertical...

Dengue Virus Type 2 Infections of Aedes aegypti Are Modulated by the Mosquito's RNA Interference Pathway

Sánchez-Vargas, Irma, Scott, Jaclyn C., Poole-Smith, B. Katherine, Franz, Alexander W. E., Barbosa-Solomieu, Valérie, Wilusz, Jeffrey, ...

A number of studies have shown that both innate and adaptive immune defense mechanisms greatly influence the course of human dengue virus (DENV) infections, but little is known about the innate...