Catherine Zwahlen

Cross-correlated chemical shift modulation: A signature of slow internal motions in proteins (2001)

Frueh, Dominique, Tolman, Joel R., Bodenhausen, Geoffrey, Zwahlen, Catherine

A novel NMR expt. allows one to characterize slow motion in macromols. The method exploits the fact that motions, such as rotation about dihedral angles, induce correlated fluctuations of the...

The conformation of NAD+ bound to lactate dehydrogenase determined by nuclear magnetic resonance with suppression of spin diffusion (1997)

Vincent, Sebastien J. F., Zwahlen, Catherine, Post, Carol Beth, Burgner, John W., Bodenhausen, Geoffrey

The conformation of NAD bound to dogfish lactate dehydrogenase (LDH) was reinvestigated by using an NMR expt. that allows one to exploit NOEs to det. internuclear distances between pairs of protons,...

Isolation of selected exchange processes in nuclear magnetic resonance (1996)

Zwahlen, Catherine, Vincent, Sebastien J. F., Schwager, Martina, Bodenhausen, Geoffrey

It is possible to inhibit the transfer of magnetization in a system with several exchanging sites in dynamic equil., as in a mixt. of cis- and trans-ZrCl4L2 with excess free ligand L (L = (CH3O)3PO)....

Measurement of cross-relaxation between amide protons in 15N-enriched proteins with suppression of spin diffusion (1996)

Vincent, Sebastien J. F., Zwahlen, Catherine, Bolton, Philip H., Logan, Timothy M., Bodenhausen, Geoffrey

A variant of two-dimensional nuclear Overhauser effect spectroscopy (NOESY) is described which allows one to observe cross-relaxation pathways between protons that have heteronuclear scalar couplings...

Suppression of spin diffusion in selected frequency bands of nuclear Overhauser spectra (1996)

Vincent, Sebastien J. F., Zwahlen, Catherine, Bodenhausen, Geoffrey

A variant of 2-dimensional nuclear Overhauser effect spectroscopy (NOESY) is described that yields information about cross-relaxation rates between pairs of spins, while the migration of...

Towards the accurate measurement of internuclear distances in biological macromolecules by suppression of spin diffusion (1996)

Vincent, Sebastien J. F., Zwahlen, Catherine, Bodenhausen, Geoffrey

The anal. of 2-dimensional NOESY spectra can be misleading if spin diffusion effects are not properly taken into account. An approach consisting in suppressing spin diffusion by selective inversion...

Generation of complementary multiplets for automated analysis of selective correlation spectra (1994)

Vincent, Sebastien J. F., Zwahlen, Catherine, Bodenhausen, Geoffrey

Passive spin filters are combined with pure in-phase correlation spectroscopy (PICSY) to obtain SIS-(p,q)-PICSY expts., where p and q are the nos. of passive spins that are simultaneously or...

Quenching spin diffusion in selective measurements of transient overhauser effects in nuclear magnetic resonance. Applications to oligonucleotides (1994)

Zwahlen, Catherine, Vincent, Sebastien J. F., Di Bari, Lorenzo, Levitt, Malcolm H., Bodenhausen, Geoffrey

In high-resoln. NMR, the transfer of longitudinal magnetization from one spin to another (A -> X) under the effect of cross relaxation NOE is often complicated by spin-diffusion pathways through...

Selective measurement of the time dependence of the transient Overhauser effect in NMR spectra; application to oligonucleotides (1994)

Vincent, Sebastien J. F., Zwahlen, Catherine, Bodenhausen, Geoffrey

A method was developed for measuring the rise and fall curves corresponding to selected peaks in NOESY spectrum. When applied to a std. two-dimensional NOESY spectrum, this technique gives result the...

Accurate determination of scalar couplings by convolution of complementary two-dimensional multiplets (1993)

Huber, Peter, Zwahlen, Catherine, Vincent, Sebastien J. F., Bodenhausen, Geoffrey

The authors describe how the value of a nuclear spin coupling const. JAX can be detd. very accurately by comparing 2 complementary 2-dimensional multiplets obtained with selective correlation NMR...

Selective magnetic resonance correlation spectroscopy with in-phase multiplets (1993)

Vincent, Sebastien J. F., Zwahlen, Catherine, Bodenhausen, Geoffrey

New 2-dimensional NMR correlation methods are presented which allow one to obtain selected high-resoln. multiplets where all signals have pos. pure absorption peak shapes. The multiplets do not...

High-resolution two-dimensional in-phase multiplets in nuclear magnetic resonance correlation spectroscopy (1992)

Vincent, Sebastien J. F., Zwahlen, Catherine, Bodenhausen, Geoffrey

It is shown how selective high resoln. 2-dimensional NMR spectra can be obtained which feature multiplets where all peaks have the same pos. sign and pure absorption lineshapes. [on SciFinder (R)]

Separation of overlapping multiplets in a two-dimensional NMR spectrum by injection of magnetization (1992)

Zwahlen, Catherine, Vincent, Sebastien J. F., Bodenhausen, Geoffrey

A SPLIT-COSY (SPLIT = sepn. of patterns by lifting of the imbroglio of transfer) technique is proposed for the sepn. of overlapping multiplets in the signal-rich region of a COSY spectra. This...

The conformation of NAD+ bound to lactate dehydrogenase determined by nuclear magnetic resonance with suppression of spin diffusion

Vincent, Sébastien J. F., Zwahlen, Catherine, Post, Carol Beth, Burgner, John W., Bodenhausen, Geoffrey

We have reinvestigated the conformation of NAD+ bound to dogfish lactate dehydrogenase (LDH) by using an NMR experiment that allows one to exploit nuclear Overhauser effects to determine internuclear...

High-affinity binding of the Drosophila Numb phosphotyrosine-binding domain to peptides containing a Gly-Pro-(p)Tyr motif

Li, Shun-Cheng, Songyang, Zhou, Vincent, Sébastien J. F., Zwahlen, Catherine, Wiley, Sandra, Cantley, Lewis, ...

The phosphotyrosine-binding (PTB) domain is a recently identified protein module that has been characterized as binding to phosphopeptides containing an NPXpY motif (X = any amino acid). We describe...

A positive feedback loop stabilizes the guanine-nucleotide exchange factor Cdc24 at sites of polarization

Butty, Anne-Christine, Perrinjaquet, Nathalie, Petit, Audrey, Jaquenoud, Malika, Segall, Jeffrey E., Hofmann, Kay, ...

In Saccharomyces cerevisiae, activation of Cdc42 by its guanine-nucleotide exchange factor Cdc24 triggers polarization of the actin cytoskeleton at bud emergence and in response to mating pheromones....

Multiple modes of peptide recognition by the PTB domain of the cell fate determinant Numb

Zwahlen, Catherine, Li, Shun-Cheng, Kay, Lewis E., Pawson, Tony, Forman-Kay, Julie D.

The phosphotyrosine-binding (PTB) domain of the cell fate determinant Numb is involved in the formation of multiple protein complexes in vivo and can bind a diverse array of peptide sequences in...

The conformation of NAD+ bound to lactate dehydrogenase determined by nuclear magnetic resonance with suppression of spin diffusion

Vincent, Sébastien J. F., Zwahlen, Catherine, Post, Carol Beth, Burgner, John W., Bodenhausen, Geoffrey

We have reinvestigated the conformation of NAD+ bound to dogfish lactate dehydrogenase (LDH) by using an NMR experiment that allows one to exploit nuclear Overhauser effects to determine internuclear...

High-affinity binding of the Drosophila Numb phosphotyrosine-binding domain to peptides containing a Gly-Pro-(p)Tyr motif

Li, Shun-Cheng, Songyang, Zhou, Vincent, Sébastien J. F., Zwahlen, Catherine, Wiley, Sandra, Cantley, Lewis, ...

The phosphotyrosine-binding (PTB) domain is a recently identified protein module that has been characterized as binding to phosphopeptides containing an NPXpY motif (X = any amino acid). We describe...

A positive feedback loop stabilizes the guanine-nucleotide exchange factor Cdc24 at sites of polarization

Butty, Anne-Christine, Perrinjaquet, Nathalie, Petit, Audrey, Jaquenoud, Malika, Segall, Jeffrey E., Hofmann, Kay, ...

In Saccharomyces cerevisiae, activation of Cdc42 by its guanine-nucleotide exchange factor Cdc24 triggers polarization of the actin cytoskeleton at bud emergence and in response to mating pheromones....

Multiple modes of peptide recognition by the PTB domain of the cell fate determinant Numb

Zwahlen, Catherine, Li, Shun-Cheng, Kay, Lewis E., Pawson, Tony, Forman-Kay, Julie D.

The phosphotyrosine-binding (PTB) domain of the cell fate determinant Numb is involved in the formation of multiple protein complexes in vivo and can bind a diverse array of peptide sequences in...