Chiatogu Onyewu

Dynamic and Heterogeneous Mutations to Fluconazole Resistance in Cryptococcus neoformans

Xu, Jianping, Onyewu, Chiatogu, Yoell, Heather J., Ali, Rabia Y., Vilgalys, Rytas J., Mitchell, Thomas G.

Infections with the human pathogenic basidiomycetous yeast Cryptococcus neoformans are often treated with fluconazole. Resistance to this antifungal agent has been reported. This study investigated...

Ergosterol Biosynthesis Inhibitors Become Fungicidal when Combined with Calcineurin Inhibitors against Candida albicans, Candida glabrata, and Candida krusei

Onyewu, Chiatogu, Blankenship, Jill R., Del Poeta, Maurizio, Heitman, Joseph

Azoles target the ergosterol biosynthetic enzyme lanosterol 14α-demethylase and are a widely applied class of antifungal agents because of their broad therapeutic window, wide spectrum of activity,...

In Vitro Interactions between Antifungals and Immunosuppressants against Aspergillus fumigatus

Steinbach, William J., Schell, Wiley A., Blankenship, Jill R., Onyewu, Chiatogu, Heitman, Joseph, Perfect, John R.

The optimal treatment for invasive aspergillosis remains elusive, despite the increased efficacy of newer agents. The immunosuppressants cyclosporine (CY), tacrolimus (FK506), and sirolimus (formerly...

The Calcineurin Target, Crz1, Functions in Azole Tolerance but Is Not Required for Virulence of Candida albicans

Onyewu, Chiatogu, Wormley, Floyd L., Perfect, John R., Heitman, Joseph

In Candida albicans, calcineurin is essential for virulence and survival during membrane perturbation by azoles. Crz1 is a proposed downstream target of calcineurin based on studies of Saccharomyces...

Dynamic and Heterogeneous Mutations to Fluconazole Resistance in Cryptococcus neoformans

Xu, Jianping, Onyewu, Chiatogu, Yoell, Heather J., Ali, Rabia Y., Vilgalys, Rytas J., Mitchell, Thomas G.

Infections with the human pathogenic basidiomycetous yeast Cryptococcus neoformans are often treated with fluconazole. Resistance to this antifungal agent has been reported. This study investigated...

Ergosterol Biosynthesis Inhibitors Become Fungicidal when Combined with Calcineurin Inhibitors against Candida albicans, Candida glabrata, and Candida krusei

Onyewu, Chiatogu, Blankenship, Jill R., Del Poeta, Maurizio, Heitman, Joseph

Azoles target the ergosterol biosynthetic enzyme lanosterol 14α-demethylase and are a widely applied class of antifungal agents because of their broad therapeutic window, wide spectrum of activity,...

In Vitro Interactions between Antifungals and Immunosuppressants against Aspergillus fumigatus

Steinbach, William J., Schell, Wiley A., Blankenship, Jill R., Onyewu, Chiatogu, Heitman, Joseph, Perfect, John R.

The optimal treatment for invasive aspergillosis remains elusive, despite the increased efficacy of newer agents. The immunosuppressants cyclosporine (CY), tacrolimus (FK506), and sirolimus (formerly...

The Calcineurin Target, Crz1, Functions in Azole Tolerance but Is Not Required for Virulence of Candida albicans

Onyewu, Chiatogu, Wormley, Floyd L., Perfect, John R., Heitman, Joseph

In Candida albicans, calcineurin is essential for virulence and survival during membrane perturbation by azoles. Crz1 is a proposed downstream target of calcineurin based on studies of Saccharomyces...

Calcineurin Promotes Infection of the Cornea by Candida albicans and Can Be Targeted To Enhance Fluconazole Therapy▿

Onyewu, Chiatogu, Afshari, Natalie A., Heitman, Joseph

In an established Candida albicans murine keratitis model, combination therapy with ophthalmic preparations of fluconazole and cyclosporine A (CsA) demonstrated in vivo drug synergy and effectively...

Targeting the Calcineurin Pathway Enhances Ergosterol Biosynthesis Inhibitors against Trichophyton mentagrophytes In Vitro and in a Human Skin Infection Model▿

Onyewu, Chiatogu, Eads, Emily, Schell, Wiley A., Perfect, John R., Ullmann, Yehuda, Kaufman, Gil, ...

Fluconazole-FK506 or fluconazole-cyclosporine drug combinations were tested in an ex vivo Trichophyton mentagrophytes human skin infection model. Conidia colonization was monitored by scanning...