Chihiro Sugimoto

Cloning and characterization of cDNA encoding a prohibitin-like protein from Theileria orientalis (2005)

He, Weiyong, Ohashi, Kazuhiko, Sugimoto, Chihiro, Tsuji, Masayoshi, Onuma, Misao

A cDNA clone encoding a prohibitin-like protein (Toprh) was isolated from a piroplasm cDNA library of Theileria orientalis and its nucleotide sequence was determined. An open reading frame, encoding...

Cloning and characterization of cDNA encoding a prohibitin-like protein from Theileria orientalis (2005)

He, Weiyong, Ohashi, Kazuhiko, Sugimoto, Chihiro, Tsuji, Masayoshi, Onuma, Misao

A cDNA clone encoding a prohibitin-like protein (Toprh) was isolated from a piroplasm cDNA library of Theileria orientalis and its nucleotide sequence was determined. An open reading frame, encoding...

Cloning and characterization of cDNA encoding a prohibitin-like protein from Theileria orientalis (2005)

He, Weiyong, Ohashi, Kazuhiko, Sugimoto, Chihiro, Tsuji, Masayoshi, Onuma, Misao

A cDNA clone encoding a prohibitin-like protein (Toprh) was isolated from a piroplasm cDNA library of Theileria orientalis and its nucleotide sequence was determined. An open reading frame, encoding...

Detection of Antibodies to Neospora caninum in Cattle by Enzyme-Linked Immunosorbent Assay with Truncated NcSRS2 Expressed in Escherichia coli (2005)

Irungu Gaturaga, Bayin Chahan, Xuenan Xuan, Xiaohong Huang, Min Liao, Shinya Fukumoto, ...

The surface antigen 1–related sequence 2 of Neospora caninum (NcSRS2) is considered as an immunodominant antigen. In this study, the gene encoding truncated NcSRS2 (NcSRS2t) lacking an N-terminal...

SERODIAGNOSIS OF CANINE BABESIA GIBSONI INFECTION BY ENZYME-LINKED IMMUNOSORBENT ASSAY WITH RECOMBINANT P50 EXPRESSED IN ESCHERICHIA COLI (2004)

Shinya Fukumoto, Yukiko Sekine, Xuenan Xuan, Ikuo Igarashi, Chihiro Sugimoto, Hideyuki Nagasawa, ...

The entire P50 gene encoding a surface protein of Babesia gibsoni was cloned into the bacteria expression vector pGEX-4T-3 and subsequently expressed in Escherichia coli as a glutathione...

Blood meal acquisition by ticks ; molecular advances and implications for vaccine development (2002)

MULENGA, Albert, TSUDA, Akiko, SUGIMOTO, Chihiro, ONUMA, Misao

In their quest for a blood meal, hematophagous arthropods must first defeat the host's hemostatic defense. Following injury as it occurs when hematophagous arthropods insert their proboscis into host...

Characterization of proteolytic enzymes expressed in the midgut of Haemaphysalis longicornis (1999)

MULENGA, Albert, SUGIMOTO, Chihiro, ONUMA, Misao

The proteolytic activities present in midguts of both fed and unfed Haemaphysalis longicornis were assessed by using the gelatin-substrate gel electrophoresis and inhibitor sensitivity analyses....

IMMUNOMODULATIVE EFFECTS OF BOVINE IMMUNODEFICIENCY-LIKE VIRUS (BIV)-INFECTION AND MIXED INFECTION OF BIV AND BOVINE LEUKEMIA VIRUS ON SHEEP (1996)

HIRAI, Nobuaki, KABEYA, Hidenori, OHASHI, Kazuhiko, SUGIMOTO, Chihiro, ONUMA, Misao

Experimental bovine immunodeficiency-like virus (BIV)-infection and mixed infection of BIV and bovine leukemia virus (BLV) were performed on sheep. BIV proviral DNA and anti-BIV antibodies were...

N-TERMINAL AMINO ACID SEQUENCE OF A 28 kDa MAJOR SERUM HIGH DENSITY LIPOPROTEIN OF THE RAINBOW TROUT ONCORHYNCHUS MYKISS (1994)

MURATA, Mie, SUGIMOTO, Chihiro, KODAMA, Hiroshi, ONUMA, Misao

As there are few data on primary structures of fish apolipoproteins (apo), we determined the N-terminal sequence of a 28 kDa major serum protein of rainbow trout, a possible homologue of apoAI...

THEILERIOSIS IN ZAMBIA : ETIOLOGY, EPIDEMIOLOGY AND CONTROL MEASURES (1994)

NAMBOTA, Andrew, SAMUI, Kenny, SUGIMOTO, Chihiro, KAKUTA, Tsutomu, ONUMA, Misao

In Zambia, theileriosis manifests itself in the form of Corridor disease (CD), caused by Theileria parva lawrencei, and East Coast fever (ECF), caused by T. parva parva. Of the approximately 3...

ANTI-TUMOR IMMUNITY AGAINST MAREK'S DISEASE-DERIVED LYMPHOBLASTOID CELL LINE (MSB-1) (1978)

SUGIMOTO, Chihiro, KODAMA, Hiroshi, MIKAMI, Takeshi

Cytotoxic effects of peripheral blood lymphocytes (PBL) and immune sera from chickens infected with Marek's disease virus (MDV) or vaccinated with herpesvirus of turkey (HVT) against Marek's disease...

Molecular Characterization of a Haemaphysalis longicornis Tick Salivary Gland-Associated 29-Kilodalton Protein and Its Effect as a Vaccine against Tick Infestation in Rabbits

Mulenga, Albert, Sugimoto, Chihiro, Sako, Yasuhito, Ohashi, Kazuhiko, Musoke, Anthony, Shubash, Mozaria, ...

The use of tick vaccines in mammalian hosts has been shown to be the most promising alternative tick control method to current use of acaricides, which suffers from a number of limitations. However,...

Cellular Localization of Babesia bovis Merozoite Rhoptry-Associated Protein 1 and Its Erythrocyte-Binding Activity

Yokoyama, Naoaki, Suthisak, Boonchit, Hirata, Haruyuki, Matsuo, Tomohide, Inoue, Noboru, Sugimoto, Chihiro, ...

The cellular localization of Babesia bovis rhoptry-associated protein 1 (RAP-1) and its erythrocyte-binding affinity were examined with anti-RAP-1 antibodies. In an indirect immunofluorescent...

Characterization of Toxoplasma gondii SAG2 Expressed in Insect Cells by Recombinant Baculovirus and Evaluation of Its Diagnostic Potential in an Enzyme-Linked Immunosorbent Assay

Huang, Xiaohong, Xuan, Xuenan, Suzuki, Hiroshi, Sugimoto, Chihiro, Nagasawa, Hideyuki, Fujisaki, Kozo, ...

A baculovirus carrying the SAG2 gene of Toxoplasma gondii was constructed, and recombinant SAG2 protein (S-rSAG2) was expressed in insect cells. S-rSAG2 was recognized by sera from cats and pigs...

Roles of the Maltese Cross Form in the Development of Parasitemia and Protection against Babesia microti Infection in Mice

Yokoyama, Naoaki, Bork, Sabine, Nishisaka, Mitsuhiro, Hirata, Haruyuki, Matsuo, Tomohide, Inoue, Noboru, ...

Babesia microti, a hemoprotozoan parasite of rodents, is also important as a zoonotic agent of human babesiosis. The Maltese cross form, which consists of four masses in an erythrocyte, is...

High-Level Expression and Purification of a Truncated Merozoite Antigen-2 of Babesia equi in Escherichia coli and Its Potential for Immunodiagnosis

Huang, Xiaohong, Xuan, Xuenan, Yokoyama, Naoaki, Xu, Longshan, Suzuki, Hiroshi, Sugimoto, Chihiro, ...

The gene encoding a truncated merozoite antigen-2 (EMA-2t) of Babesia equi was cloned and highly expressed in Escherichia coli as a glutathione S-transferase fusion protein (G-rEMA-2t). Both...

High-Level Expression of Truncated Surface Antigen P50 of Babesia gibsoni in Insect Cells by Baculovirus and Evaluation of Its Immunogenicity and Antigenicity

Fukumoto, Shinya, Xuan, Xuenan, Kadota, Kimie, Igarashi, Ikuo, Sugimoto, Chihiro, Fujisaki, Kozo, ...

Previously, we identified an immunodominant antigen, P50 of Babesia gibsoni. In the present study, the gene encoding the truncated P50 (rP50t) without a C-terminal hydrophobic region (29 amino acids...

Cloning and Expression of Mitochondrial Heat Shock Protein 70 of Trypanosoma congolense and Potential Use as a Diagnostic Antigen

Bannai, Hiroshi, Sakurai, Tatsuya, Inoue, Noboru, Sugimoto, Chihiro, Igarashi, Ikuo

The ability to use mitochondrial heat shock protein 70 (MTP) of Trypanosoma congolense as a diagnostic antigen was examined. One cDNA clone was obtained by immunoscreening of a T. congolense...

Loop-Mediated Isothermal Amplification for Detection of African Trypanosomes

Kuboki, Noritaka, Inoue, Noboru, Sakurai, Tatsuya, Di Cello, Francescopaolo, Grab, Dennis J., Suzuki, Hiroshi, ...

While PCR is a method of choice for the detection of African trypanosomes in both humans and animals, the expense of this method negates its use as a diagnostic method for the detection of endemic...

Growth-Inhibitory Effect of Heparin on Babesia Parasites

Bork, Sabine, Yokoyama, Naoaki, Ikehara, Yuzuru, Kumar, Sanjay, Sugimoto, Chihiro, Igarashi, Ikuo

We examined the inhibitory effects of three heparins on the growth of Babesia parasites. The multiplication of Babesia bovis, B. bigemina, B. equi, and B. caballi in in vitro cultures and that of B....

Inhibitory Effect of Antiserum to Surface Antigen P50 of Babesia gibsoni on Growth of Parasites in Severe Combined Immunodeficiency Mice Given Canine Red Blood Cells

Fukumoto, Shinya, Xuan, Xuenan, Takabatake, Noriyuki, Igarashi, Ikuo, Sugimoto, Chihiro, Fujisaki, Kozo, ...

The inhibitory effect of an antiserum to surface protein P50 of Babesia gibsoni on the growth of the parasite was determined with severe combined immunodeficiency mice given canine red blood cells....

Molecular Characterization of a Haemaphysalis longicornis Tick Salivary Gland-Associated 29-Kilodalton Protein and Its Effect as a Vaccine against Tick Infestation in Rabbits

Mulenga, Albert, Sugimoto, Chihiro, Sako, Yasuhito, Ohashi, Kazuhiko, Musoke, Anthony, Shubash, Mozaria, ...

The use of tick vaccines in mammalian hosts has been shown to be the most promising alternative tick control method to current use of acaricides, which suffers from a number of limitations. However,...

Cellular Localization of Babesia bovis Merozoite Rhoptry-Associated Protein 1 and Its Erythrocyte-Binding Activity

Yokoyama, Naoaki, Suthisak, Boonchit, Hirata, Haruyuki, Matsuo, Tomohide, Inoue, Noboru, Sugimoto, Chihiro, ...

The cellular localization of Babesia bovis rhoptry-associated protein 1 (RAP-1) and its erythrocyte-binding affinity were examined with anti-RAP-1 antibodies. In an indirect immunofluorescent...

Characterization of Toxoplasma gondii SAG2 Expressed in Insect Cells by Recombinant Baculovirus and Evaluation of Its Diagnostic Potential in an Enzyme-Linked Immunosorbent Assay

Huang, Xiaohong, Xuan, Xuenan, Suzuki, Hiroshi, Sugimoto, Chihiro, Nagasawa, Hideyuki, Fujisaki, Kozo, ...

A baculovirus carrying the SAG2 gene of Toxoplasma gondii was constructed, and recombinant SAG2 protein (S-rSAG2) was expressed in insect cells. S-rSAG2 was recognized by sera from cats and pigs...

Roles of the Maltese Cross Form in the Development of Parasitemia and Protection against Babesia microti Infection in Mice

Yokoyama, Naoaki, Bork, Sabine, Nishisaka, Mitsuhiro, Hirata, Haruyuki, Matsuo, Tomohide, Inoue, Noboru, ...

Babesia microti, a hemoprotozoan parasite of rodents, is also important as a zoonotic agent of human babesiosis. The Maltese cross form, which consists of four masses in an erythrocyte, is...

High-Level Expression and Purification of a Truncated Merozoite Antigen-2 of Babesia equi in Escherichia coli and Its Potential for Immunodiagnosis

Huang, Xiaohong, Xuan, Xuenan, Yokoyama, Naoaki, Xu, Longshan, Suzuki, Hiroshi, Sugimoto, Chihiro, ...

The gene encoding a truncated merozoite antigen-2 (EMA-2t) of Babesia equi was cloned and highly expressed in Escherichia coli as a glutathione S-transferase fusion protein (G-rEMA-2t). Both...

High-Level Expression of Truncated Surface Antigen P50 of Babesia gibsoni in Insect Cells by Baculovirus and Evaluation of Its Immunogenicity and Antigenicity

Fukumoto, Shinya, Xuan, Xuenan, Kadota, Kimie, Igarashi, Ikuo, Sugimoto, Chihiro, Fujisaki, Kozo, ...

Previously, we identified an immunodominant antigen, P50 of Babesia gibsoni. In the present study, the gene encoding the truncated P50 (rP50t) without a C-terminal hydrophobic region (29 amino acids...

Cloning and Expression of Mitochondrial Heat Shock Protein 70 of Trypanosoma congolense and Potential Use as a Diagnostic Antigen

Bannai, Hiroshi, Sakurai, Tatsuya, Inoue, Noboru, Sugimoto, Chihiro, Igarashi, Ikuo

The ability to use mitochondrial heat shock protein 70 (MTP) of Trypanosoma congolense as a diagnostic antigen was examined. One cDNA clone was obtained by immunoscreening of a T. congolense...

Loop-Mediated Isothermal Amplification for Detection of African Trypanosomes

Kuboki, Noritaka, Inoue, Noboru, Sakurai, Tatsuya, Di Cello, Francescopaolo, Grab, Dennis J., Suzuki, Hiroshi, ...

While PCR is a method of choice for the detection of African trypanosomes in both humans and animals, the expense of this method negates its use as a diagnostic method for the detection of endemic...

Growth-Inhibitory Effect of Heparin on Babesia Parasites

Bork, Sabine, Yokoyama, Naoaki, Ikehara, Yuzuru, Kumar, Sanjay, Sugimoto, Chihiro, Igarashi, Ikuo

We examined the inhibitory effects of three heparins on the growth of Babesia parasites. The multiplication of Babesia bovis, B. bigemina, B. equi, and B. caballi in in vitro cultures and that of B....

Inhibitory Effect of Antiserum to Surface Antigen P50 of Babesia gibsoni on Growth of Parasites in Severe Combined Immunodeficiency Mice Given Canine Red Blood Cells

Fukumoto, Shinya, Xuan, Xuenan, Takabatake, Noriyuki, Igarashi, Ikuo, Sugimoto, Chihiro, Fujisaki, Kozo, ...

The inhibitory effect of an antiserum to surface protein P50 of Babesia gibsoni on the growth of the parasite was determined with severe combined immunodeficiency mice given canine red blood cells....

Common inheritance of chromosome Ia associated with clonal expansion of Toxoplasma gondii

Khan, Asis, Böhme, Ulrike, Kelly, Krystyna A., Adlem, Ellen, Brooks, Karen, Simmonds, Mark, ...

Toxoplasma gondii is a globally distributed protozoan parasite that can infect virtually all warm-blooded animals and humans. Despite the existence of a sexual phase in the life cycle, T. gondii has...

Effects of Exogenous Transforming Growth Factor β on Trypanosoma congolense Infection in Mice▿

Namangala, Boniface, Sugimoto, Chihiro, Inoue, Noboru

The socioeconomic implications of trypanosomosis in sub-Saharan Africa and the limitations of its current control regimes have stimulated research into alternative control methods. Considering the...