Shikata, Koh, Ohta, Satoshi, Yamada, Kouichi, Obuse, Chikashi, Yoshikawa, Hiroshi, Tsurimoto, Toshiki
An essential eukaryotic DNA polymerase, DNA polymerase δ(pol δ), synthesizes DNA processively in the presence of proliferating cell nuclear antigen (PCNA). Recently, a 66 kDa polypeptide (p66) that...
ATP-dependent structural change of the eukaryotic clamp-loader protein, replication factor C
Shiomi, Yasushi, Usukura, Jiro, Masamura, Yusuke, Takeyasu, Kunio, Nakayama, Yoshikazu, Obuse, Chikashi, ...
The eukaryotic DNA sliding clamp that keeps DNA polymerase engaged at a replication fork, called proliferating cell nuclear antigen (PCNA), is loaded onto the 3′ ends of primer DNA through its...
The role of Ppe1/PP6 phosphatase for equal chromosome segregation in fission yeast kinetochore
Goshima, Gohta, Iwasaki, Osamu, Obuse, Chikashi, Yanagida, Mitsuhiro
Mis12 is a kinetochore protein essential for equal chromosome segregation and is evolutionarily conserved from yeast to human. In this study, we report the isolation and characterization of...
ATP-dependent structural change of the eukaryotic clamp-loader protein, replication factor C
Shiomi, Yasushi, Usukura, Jiro, Masamura, Yusuke, Takeyasu, Kunio, Nakayama, Yoshikazu, Obuse, Chikashi, ...
The eukaryotic DNA sliding clamp that keeps DNA polymerase engaged at a replication fork, called proliferating cell nuclear antigen (PCNA), is loaded onto the 3′ ends of primer DNA through its...
The role of Ppe1/PP6 phosphatase for equal chromosome segregation in fission yeast kinetochore
Goshima, Gohta, Iwasaki, Osamu, Obuse, Chikashi, Yanagida, Mitsuhiro
Mis12 is a kinetochore protein essential for equal chromosome segregation and is evolutionarily conserved from yeast to human. In this study, we report the isolation and characterization of...
Two E3 ubiquitin ligases, SCF-Skp2 and DDB1-Cul4, target human Cdt1 for proteolysis
Nishitani, Hideo, Sugimoto, Nozomi, Roukos, Vassilis, Nakanishi, Yohsuke, Saijo, Masafumi, Obuse, Chikashi, ...
Replication licensing is carefully regulated to restrict replication to once in a cell cycle. In higher eukaryotes, regulation of the licensing factor Cdt1 by proteolysis and Geminin is essential to...
Uehara, Ryota, Nozawa, Ryu-suke, Tomioka, Akiko, Petry, Sabine, Vale, Ronald D., Obuse, Chikashi, ...
The mitotic spindle is constructed from microtubules (MTs) nucleated from centrosomes, chromosome proximal regions, and preexisting spindle MTs. Augmin, a recently identified protein complex, is a...
Active establishment of centromeric CENP-A chromatin by RSF complex
Perpelescu, Marinela, Nozaki, Naohito, Obuse, Chikashi, Yang, Hua, Yoda, Kinya
Centromeres are chromosomal structures required for equal DNA segregation to daughter cells, comprising specialized nucleosomes containing centromere protein A (CENP-A) histone, which provide the...