Pretz, Monika G., Does, Chris Van Der, Albers, Sonja-Verena, Schuurman-Wolters, Gea, Driessen, Arnold J.M.
GlcV is the nucleotide binding domain of the ABC-type glucose transporter of the hyperthermoacidophile Sulfolobus solfataricus. GlcV consists of two domains, an N-terminal domain containing the...
Hofacker, Matthias, Gompf, Simone, Zutz, Ariane, Presenti, Chiara, Haase, Winfried, Does, Chris Van Der, ...
The ATP-binding cassette half-transporter Mdl1 from Saccharomyces cerevisiae has been proposed to be involved in the quality control of misassembled respiratory chain complexes by exporting...
Gompf, Simone, Zutz, Ariane, Hofacker, Matthias, Haase, Winfried, Does, Chris Van Der, Tampé, Robert
The ATP-binding cassette transporter MDL1 of Saccharomyces cerevisiae has been implicated in mitochondrial quality control, exporting degradation products of misassembled respiratory chain complexes....
Salgado-Pabón, Wilmara, Jain, Samta, Turner, Nicholas, Does, Chris Van Der, Dillard, Joseph P.
The Neisseria gonorrhoeae type IV secretion system secretes chromosomal DNA that acts in natural transformation. To examine the mechanism of DNA processing for secretion, we made mutations in the...
Salgado-Pabón, Wilmara, Jain, Samta, Turner, Nicholas, Does, Chris Van Der, Dillard, Joseph P.
The Neisseria gonorrhoeae type IV secretion system secretes chromosomal DNA that acts in natural transformation. To examine the mechanism of DNA processing for secretion, we made mutations in the...
Pretz, Monika G., Albers, Sonja-Verena, Schuurman-Wolters, Gea, Tampé, Robert, Driessen, Arnold J.M., Does, Chris Van Der
ATP-binding cassette transporters drive the transport of substrates across the membrane by the hydrolysis of ATP. They typically have a conserved domain structure with two membrane-spanning domains...
Nouwen, Nico, Koch, Joachim, Keyzer, Jeanine De, Does, Chris Van Der, Tampé, Robert, ...
The motor protein SecA drives the translocation of (pre-)proteins across the SecYEG channel in the bacterial cytoplasmic membrane by nucleotide-dependent cycles of conformational changes often...
Does, Chris Van Der, Presenti, Chiara, Schulze, Katrin, Dinkelaker, Stephanie, Tampé, Robert
We have recently proposed a “processive clamp” model for the ATP hydrolysis cycle of the nucleotide-binding domain (NBD) of the mitochondrial ABC transporter Mdl1. In this model, ATP binding to...
Natale, Paolo, Blaauwen, Tanneke Den, Does, Chris Van Der, Driessen, Arnold J.M.
The SecYEG complex is a membrane-embedded channel that permits the passage of precursor proteins (preproteins) across the inner membrane of Escherichia coli. SecA is a molecular motor that associates...
The Oligomeric Distribution of SecYEG is Altered by SecA and Translocation Ligands (2005)
Scheuring, Johannes, Braun, Nathalie, Nothdurft, Lars, Stumpf, Matthias, Veenendaal, Andreas K.J., Kol, Stefan, ...
The multimeric membrane protein complex translocase mediates the transport of preproteins across and integration of membrane proteins into the inner membrane of Escherichia coli. The translocase...
Covalently Dimerized SecA Is Functional in Protein Translocation (2005)
Keyzer, Jeanine De, Spelbrink, Robin E.J., Nijstad, Niels, Kruijff, Ben De, Nouwen, Nico, ...
The ATPase SecA provides the driving force for the transport of secretory proteins across the cytoplasmic membrane of Escherichia coli. SecA exists as a dimer in solution, but the exact oligomeric...
Plantinga, Titia H., Does, Chris Van Der, Tomkiewicz, Danuta, Keulen, Geertje Van, Konings, Wil N., Driessen, Arnold J.M.
Binding-protein-dependent secondary transporters make up a unique transport protein family. They use a solute-binding protein in proton-motive-force-driven transport. Only a few systems have been...
The protein-conducting channel SecYEG (2004)
Veenendaal, Andreas K.J., Does, Chris Van Der, Driessen, Arnold J.M.
In bacteria, the translocase mediates the translocation of proteins into or across the cytosolic membrane. It consists of a membrane embedded protein-conducting channel and a peripherally associated...
Transporter’s evolution and carbohydrate metabolic clusters (2004)
Plantinga, Titia H., Does, Chris Van Der, Driessen, Arnold J.M.
The yiaQRS genes of Escherichia coli K-12 are involved in carbohydrate metabolism. Clustering of homologous genes was found throughout several unrelated bacteria. Strikingly, all four bacterial...
Binding of SecA to the SecYEG Complex Accelerates the Rate of Nucleotide Exchange on SecA (2004)
Natale, Paolo, Swaving, Jelto, Does, Chris Van Der, Keyzer, Jeanine De, Driessen, Arnold J.M.
SecYEG translocase mediates the transport of preproteins across the inner membrane of Escherichia coli. SecA binds the membrane-embedded SecYEG protein-conducting channel with high affinity and then...
Functional characterization of the Escherichia coli K-12 yiaMNO transport protein genes (2004)
Plantinga, Titia H., Does, Chris Van Der, Badia, Josefa, Aguilar, Juan, Konings, Wil N., Driessen, Arnold J.M.
The yiaMNO genes of Escherichia coli K-12 encode a binding protein-dependent secondary, or tri-partite ATP-independent periplasmic (TRAP), transporter. Since only a few members of this family have...
How do ABC transporters drive transport? (2004)
Does, Chris Van Der, Tampé, Robert
Members of the ATP-binding cassette (ABC) superfamily are integral membrane proteins that hydrolyze ATP to drive transport. In the last two decades these proteins have been extensively characterized...
Keyzer, Jeanine De, Does, Chris Van Der, Kloosterman, Tomas G., Driessen, Arnold J.M.
Translocase mediates the transport of preproteins across the inner membrane of Escherichia coli. SecA binds with high affinity to the membrane-embedded protein-conducting SecYEG complex and serves as...
Purification and characterisation of a lactococcal aminoacylase (2003)
Curley, Peter, Does, Chris Van Der, Driessen, Arnold J.M., Kok, Jan, Sinderen, D. Van
The amd1-encoded aminoacylase from Lactococcus lactis MG1363 was cloned and overexpressed in Escherichia coli and purified. The assumed dimeric enzyme has a subunit molecular mass of about 42 kDa and...
Janas, Eva, Hofacker, Matthias, Chen, Min, Gompf, Simone, Does, Chris Van Der, Tampé, Robert
The ABC transporter Mdl1p, a structural and functional homologue of the transporter associated with antigen processing (TAP) plays an important role in intracellular peptide transport from the...
Reconstitution of Purified Bacterial Preprotein Translocase in Liposomes (2003)
Does, Chris Van Der, Keyzer, Jeanine De, Laan, Martin Van Der, Driessen, Arnold J.M.
Keyzer, Jeanine De, Does, Chris Van Der, Swaving, Jelto, Driessen, Arnold J.M.
SecYEG forms the protein-conducting channel of the Escherichia coli translocase. It binds the peripheral ATPase SecA that drives the preprotein translocation reaction. PrlA4 is a double mutant of...
Keyzer, Jeanine De, Does, Chris Van Der, Driessen, Arnold J.M.
Protein secretion in Escherichia coli is mediated by translocase, a multi-subunit membrane protein complex with SecA as ATP-driven motor protein and the SecYEG complex as translocation pore. A...
The Core of the Bacterial Translocase Harbors a Tilted Transmembrane Segment 3 of SecE (2002)
Veenendaal, Andreas K.J., Does, Chris Van Der, Driessen, Arnold J.M.
The bacterial translocase mediates the translocation and membrane integration of proteins. The integral membrane proteins SecY and SecE are conserved core subunits of the translocase. Previous...
Mapping the Sites of Interaction between SecY and SecE by Cysteine Scanning Mutagenesis (2001)
Veenendaal, Andreas K.J., Does, Chris Van Der, Driessen, Arnold J.M.
In Escherichia coli, the SecYEG complex mediates the translocation and membrane integration of proteins. Both genetic and biochemical data indicate interactions of several transmembrane segments...
The structural basis of protein targeting and translocation in bacteria (2001)
Driessen, Arnold J.M., Manting, Erik H., Does, Chris Van Der
In Gram-negative bacteria, two distinct targeting routes assist in the proper localization of secreted and membrane proteins. Signal recognition particle (SRP) mainly targets ribosome-bound nascent...
Non-bilayer Lipids Stimulate the Activity of the Reconstituted Bacterial Protein Translocase (2000)
Does, Chris Van Der, Swaving, Jelto, Klompenburg, Wim Van, Driessen, Arnold J.M.
To determine the phospholipid requirement of the preprotein translocase in vitro, the Escherichia coli SecYEG complex was purified in a delipidated form using the detergent dodecyl maltoside. SecYEG...
SecYEG assembles into a tetramer to form the active protein translocation channel (2000)
Manting, Erik H., Does, Chris Van Der, Remigy, Hervé, Engel, Andreas, Driessen, Arnold J.M.
Translocase mediates preprotein translocation across the Escherichia coli inner membrane. It consists of the SecYEG integral membrane protein complex and the peripheral ATPase SecA. Here we show by...
Scotti, Pier A., Urbanus, Malene L., Brunner, Josef, Heijne, Gunnar Von, Does, Chris Van Der, ...
In Escherichia coli, both secretory and inner membrane proteins initially are targeted to the core SecYEG inner membrane translocase. Previous work has also identified the peripherally associated...
Kaufmann, Andreas, Manting, Erik H., Veenendaal, Andreas K.J., Driessen, Arnold J.M., Does, Chris Van Der
Preprotein translocation in Escherichia coli is mediated by translocase, a multimeric membrane protein complex with SecA as the peripheral ATPase and SecYEG as the translocation pore. Unique...
Blaauwen, Tanneke Den, Does, Chris Van Der, Driessen, Arnold J.M.
SecA is the dissociatable nucleotide and preprotein binding subunit of the bacterial translocase. The thermodynamics of nucleotide binding to soluble SecA at nucleotide binding site I were determined...
A Single Amino Acid Substitution in SecY Stabilizes the Interaction with SecA (1999)
Manting, Erik H., Kaufmann, Andreas, Does, Chris Van Der, Driessen, Arnold J.M.
The SecYEG complex constitutes a protein conducting channel across the bacterial cytoplasmic membrane. It binds the peripheral ATPase SecA to form the translocase. When isoleucine 278 in...
Translocase-Bound SecA Is Largely Shielded from the Phospholipid Acyl Chains (1998)
Voorst, Frank Van, Does, Chris Van Der, Brunner, Josef, Driessen, Arnold J.M., Kruijff, Ben De
Protein translocation in Escherichia coli is mediated by the SecA ATPase bound to the SecYEG membrane protein complex. SecA translocation ATPase activity as well as protein translocation is dependent...
Does, Chris Van Der, Manting, Erik H., Kaufmann, Andreas, Lutz, Marco, Driessen, Arnold J.M.
Preprotein translocation in Escherichia coli is mediated by the translocase with SecA as peripheral ATPase and SecY, SecE, and SecG as membrane domain. To facilitate large-scale purification of the...
The positive inside rule is not determined by the polarity of Δψ (1998)
Albers, Sonja-Verena, Does, Chris Van Der, Driessen, Arnold J.M., Klompenburg, Wim Van
Blaauwen, Tanneke Den, Wit, Janny G. De, Gosker, Harry, Does, Chris Van Der, Breukink, Eef-Jan, Leij, Lou De, ...
SecA is the peripheral subunit of the preprotein translocase of Escherichia coli. SecA consists of two independently folding domains, i.e., the N-domain bearing the high-affinity nucleotide binding...
Manting, Erik H., Does, Chris Van Der, Driessen, Arnold J.M.
Precursor protein translocation across the Escherichia coli inner membrane is mediated by the translocase, which is composed of a heterotrimeric integral membrane protein complex with SecY, SecE, and...
Fekkes, Peter, Does, Chris Van Der, Driessen, Arnold J.M.
The chaperone SecB keeps precursor proteins in a translocation-competent state and targets them to SecA at the translocation sites in the cytoplasmic membrane of Escherichia coli. SecA is thought to...
Cation and sugar selectivity determinants in a novel family of transport proteins (1996)
Pourcher, Thierry, Leblanc, Gérard, Liang, Wei-Jun, Henderson, Peter J.F., Does, Chris Van Der, Knol, Jan, ...
A new family of homologous membrane proteins that transport galactosides–pentoses–hexuronides (GPH) is described. By analysing the aligned amino acid sequences of the GPH family, and by...
Does, Chris Van Der, Blaauwen, Tanneke Den, Wit, Janny G. De, Manting, Erik H., Groot, Nancy A., Fekkes, Peter, ...
SecA is the dissociable ATPase subunit of the Escherichia coli preprotein translocase, and cycles in a nucleotide-modulated manner between the cytosol and the membrane. Overproduction of the integral...
Cation and sugar selectivity determinants in a novel family of transport proteins (1996)
Henderson, Peter J.F., Does, Chris Van Der, Knol, Jan, Poolman, Bert, Liang, Wei-Jun, Leblanc, Gérard, ...
A new family of homologous membrane proteins that transport galactosides–pentoses–hexuronides (GPH) is described. By analysing the aligned amino acid sequences of the GPH family, and by...
Structure and induction pattern of a novel proteinase inhibitor class II gene of tobacco (1995)
Balandin, Teresa, Does, Chris Van Der, Bellés Albert, José-Maria, Bol, John F., Linthorst, Huub J.M.
A cDNA and a corresponding genomic clone encoding a protein with partial identity to type II proteinase inhibitors from potato, tomato and Nicotiana alata, were isolated from tobacco libraries. The...
Circadian expression and induction by wounding of tobacco genes for cysteine proteinase (1993)
Linthorst, Huub J.M., Does, Chris Van Der, Brederode, Frans Th., Bol, John F.
Two sets of clones were isolated from a tobacco cDNA library, utilizing as a probe a PCR fragment obtained from tomato cDNA using a degenerate primer based on the sequence of tomato systemin....
Tobacco proteinase inhibitor I genes are locally, but not systemically induced by stress (1993)
Linthorst, Huub J.M., Brederode, Frans Th., Does, Chris Van Der, Bol, John F.
A cDNA library of tobacco mosaic virus (TMV)-infected tobacco was screened with polymerase chain reaction products obtained using a degenerate primer corresponding to proteinase inhibitor I (PI-I) of...