Azza, Saïd, Cambillau, Christian, Raoult, Didier, Suzan-Monti, Marie
Abstract Background Acanthamoebae polyphaga Mimivirus (APM) is the largest known dsDNA virus. The viral particle has a nearly icosahedral structure with an internal capsid shell surrounded with a...
Direct in Vivo Screening of Intrabody Libraries Constructed on a (2007)
Annemarie Honegger, Christian Cambillau, Dominik Escher, Andreas Plückthun
Single-chain Fv antibody fragments (scFv) represent a convenient antibody format for intracellular expression in eukaryotic or prokaryotic cells. These so-called intrabodies have great potential in...
2 Architecture et Fonction des Macromolecules Biologiques (2007)
Annette C. Langedijk, Silvia Spinelli, Christelle Anguille, Pim Hermans, Janny Nederlof, Jens Buten, ...
*Corresponding authors
2 Architecture et Fonction des (2007)
Joè Rg Burmester, Silvia Spinelli, Luisa Pugliese, Anke Krebber, Annemarie Honegger, Sabine Jung, ...
Single-chain Fv (scFv) antibody libraries were constructed from mice immunized with an ampicillin-bovine serum albumin conjugate. Several antibodies with speci®city for intact ampicillin were...
2 Architecture et Fonction des (2007)
V H Domains, Sabine Jung, Silvia Spinelli, Bernhard Schimmele, Annemarie Honegger, Luisa Pugliese, ...
E-mail address of the corresponding author:
Improved crystallization of the coxsackievirus B3 RNA-dependent RNA polymerase (2007)
Selisko, Barbara, Coutard, Bruno, De Palma, Armando M, Egloff, Marie-Pierre, ...
The Picornaviridae virus family contains a large number of human pathogens such as poliovirus, hepatitis A virus and rhinoviruses. Amongst the viruses belonging to the genus Enterovirus, several...
Crystal structure of AFV3-109, a highly conserved protein from crenarchaeal viruses (2007)
Keller, Jenny, Leulliot, Nicolas, Cambillau, Christian, Campanacci, Valérie, Porciero, Stéphanie, Prangishvili, David, ...
Abstract The extraordinary morphologies of viruses infecting hyperthermophilic archaea clearly distinguish them from bacterial and eukaryotic viruses. Moreover, their genomes code for proteins that...
Taussig, Michael J., Stoevesandt, Oda, Bradbury, Andrew R., Cahill, Dolores, Cambillau, Christian, ...
ProteomeBinders is a new European consortium aiming to establish a comprehensive resource of well-characterized affinity reagents, including but not limited to antibodies, for analysis of the human...
Lundstrom, Kenneth, Wagner, Renaud, Reinhart, Christoph, Desmyter, Aline, Cherouati, Nadia, Magnin, Thierry, ...
Honegger, Annemarie, Spinelli, Silvia, Cambillau, Christian, Plückthun, Andreas
The structure of the scFv fragment FITC-E2, obtained from a naive phage antibody scFv library derived from human donors, was determined at 2.1 Å resolution in the free form and at 3.0 Å in the...
Low Picomolar Affinity, Christian Zahnd, Silvia Spinelli, Béatrice Luginbühl, Patrick Amstutz, Christian Cambillau, ...
We generated a single chain Fv fragment of an antibody (scFv) with a binding affinity of about 5 pM to a short peptide by applying rigorous directed evolution. Starting from a high affinity peptide...
Blaise, Mickaël, Becker, Hubert Dominique, Keith, Gérard, Cambillau, Christian, Lapointe, Jacques, Giegé, Richard, ...
Escherichia coli encodes YadB, a protein displaying 34% identity with the catalytic core of glutamyl‐tRNA synthetase but lacking the anticodon‐binding domain. We show that YadB is a...
High-throughput automated refolding screening of inclusion bodies (2004)
Vincentelli, Renaud, Canaan, Stéphane, Campanacci, Valérie, Valencia, Christel, Maurin, Damien, Frassinetti, Frédéric, ...
One of the main stumbling blocks encountered when attempting to express foreign proteins in Escherichia coli is the occurrence of amorphous aggregates of misfolded proteins, called inclusion bodies...
Biophysical properties of camelid VHH domains compared to those of human V H3 domains (2002)
Stefan Ewert, Christian Cambillau, Katja Conrath, Andreas Plückthun
ABSTRACT: Camelidae possess an unusual form of antibodies lacking the light chains. The variable domain of these heavy chain antibodies (VHH) is not paired, while the VH domain of all other...
Romier, Christophe, Bernassau, Jean-Marie, Cambillau, Christian, Darbon, Hervé
The structure of human corticotropin releasing factor (hCRF) has been determined by proton nuclear magnetic resonance (1H NMR) in a mixed-solvent system of 66% trifluoroethanol/34%...
Martinez, Chrislaine, De Geus, Pieter, Stanssens, Patrick, Lauwereys, Marc, Cambillau, Christian
Cutinases are extracellular enzymes involved in the disruption of cutine, an insoluble polyester which covers the surface of plants. They belong to a class of serine esterases that are able to...
Cutruzzolà, Francesca, Brown, Kieron, Wilson, Emma K., Bellelli, Andrea, Arese, Marzia, Tegoni, Mariella, ...
Cd1 nitrite reductase catalyzes the conversion of nitrite to NO in denitrifying bacteria. Reduction of the substrate occurs at the d1-heme site, which faces on the distal side some residues thought...
Campanacci, Valérie, Lartigue, Audrey, Hällberg, B. Martin, Jones, T. Alwyn, Giudici-Orticoni, Marie-Therèse, Tegoni, Mariella, ...
Chemosensory proteins (CSPs) have been proposed to transport hydrophobic chemicals from air to olfactory or taste receptors. They have been isolated from several sensory organs of a wide range of...
Egloff, Marie-Pierre, Ferron, François, Campanacci, Valérie, Longhi, Sonia, Rancurel, Corinne, Dutartre, Hélène, ...
The recently identified etiological agent of the severe acute respiratory syndrome (SARS) belongs to Coronaviridae (CoV), a family of viruses replicating by a poorly understood mechanism. Here, we...
A minimalist glutamyl-tRNA synthetase dedicated to aminoacylation of the tRNAAsp QUC anticodon
Blaise, Mickaël, Becker, Hubert Dominique, Keith, Gérard, Cambillau, Christian, Lapointe, Jacques, Giegé, Richard, ...
Escherichia coli encodes YadB, a protein displaying 34% identity with the catalytic core of glutamyl-tRNA synthetase but lacking the anticodon-binding domain. We show that YadB is a tRNA modifying...
Dubois, Daniel Y., Blaise, Mickaël, Becker, Hubert D., Campanacci, Valérie, Keith, Gérard, Giegé, Richard, ...
The product of the Escherichia coli yadB gene is homologous to the N-terminal part of bacterial glutamyl-tRNA synthetases (GluRSs), including the Rossmann fold with the acceptor-binding domain and...
Isolation of Llama Antibody Fragments for Prevention of Dandruff by Phage Display in Shampoo
Dolk, Edward, Van Der Vaart, Marcel, Lutje Hulsik, David, Vriend, Gert, De Haard, Hans, Spinelli, Silvia, ...
As part of research exploring the feasibility of using antibody fragments to inhibit the growth of organisms implicated in dandruff, we isolated antibody fragments that bind to a cell surface protein...
Control of domain swapping in bovine odorant-binding protein.
Ramoni, Roberto, Vincent, Florence, Ashcroft, Alison E, Accornero, Paolo, Grolli, Stefano, Valencia, Christel, ...
As revealed by the X-ray structure, bovine odorant-binding protein (OBPb) is a domain swapped dimer [Tegoni, Ramoni, Bignetti, Spinelli and Cambillau (1996) Nat. Struct. Biol. 3, 863-867; Bianchet,...
Ferrat, Gilles, Renisio, Jean-Guillaume, Morelli, Xavier, Slootstra, Jerry, Meloen, Rob, Cambillau, Christian, ...
The X-ray structure of a ternary complex between human chorionic gonadotropin hormone (hCG) and two Fvs recognizing its alpha and beta subunits has been recently determined. The Fvs recognize the...
Solution structure of a chemosensory protein from the moth Mamestra brassicae.
Mosbah, Amor, Campanacci, Valérie, Lartigue, Audrey, Tegoni, Mariella, Cambillau, Christian, Darbon, Hervé
Chemosensory proteins (CSPs) are believed to be involved in chemical communication and perception. A number of such proteins, of molecular mass approximately 13 kDa, have been isolated from different...
Crystal structure of nitrous oxide reductase from Paracoccus denitrificans at 1.6 A resolution.
Haltia, Tuomas, Brown, Kieron, Tegoni, Mariella, Cambillau, Christian, Saraste, Matti, Mattila, Kimmo, ...
N2O is generated by denitrifying bacteria as a product of NO reduction. In denitrification, N2O is metabolized further by the enzyme N2O reductase (N2OR), a multicopper protein which converts N2O...
Rivière, Stéphane, Lartigue, Audrey, Quennedey, Brigitte, Campanacci, Valérie, Farine, Jean-Pierre, Tegoni, Mariella, ...
Odorant-binding proteins (OBPs) are thought to transport volatile compounds from air to their receptors through the sensillary lymph. In this protein family, the subgroup of pheromone-binding...
Tremblay, Denise M., Tegoni, Mariella, Spinelli, Silvia, Campanacci, Valérie, Blangy, Stéphanie, Huyghe, Céline, ...
Phage p2, a member of the lactococcal 936 phage species, infects Lactococcus lactis strains by binding initially to specific carbohydrate receptors using its receptor-binding protein (RBP). The...
Minskaia, Ekaterina, Hertzig, Tobias, Gorbalenya, Alexander E., Campanacci, Valérie, Cambillau, Christian, Canard, Bruno, ...
Replication of the giant RNA genome of severe acute respiratory syndrome (SARS) coronavirus (CoV) and synthesis of as many as eight subgenomic (sg) mRNAs are mediated by a viral...
Cutruzzolà, Francesca, Brown, Kieron, Wilson, Emma K., Bellelli, Andrea, Arese, Marzia, Tegoni, Mariella, ...
Cd1 nitrite reductase catalyzes the conversion of nitrite to NO in denitrifying bacteria. Reduction of the substrate occurs at the d1-heme site, which faces on the distal side some residues thought...
Campanacci, Valérie, Lartigue, Audrey, Hällberg, B. Martin, Jones, T. Alwyn, Giudici-Orticoni, Marie-Therèse, Tegoni, Mariella, ...
Chemosensory proteins (CSPs) have been proposed to transport hydrophobic chemicals from air to olfactory or taste receptors. They have been isolated from several sensory organs of a wide range of...
Egloff, Marie-Pierre, Ferron, François, Campanacci, Valérie, Longhi, Sonia, Rancurel, Corinne, Dutartre, Hélène, ...
The recently identified etiological agent of the severe acute respiratory syndrome (SARS) belongs to Coronaviridae (CoV), a family of viruses replicating by a poorly understood mechanism. Here, we...
A minimalist glutamyl-tRNA synthetase dedicated to aminoacylation of the tRNAAsp QUC anticodon
Blaise, Mickaël, Becker, Hubert Dominique, Keith, Gérard, Cambillau, Christian, Lapointe, Jacques, Giegé, Richard, ...
Escherichia coli encodes YadB, a protein displaying 34% identity with the catalytic core of glutamyl-tRNA synthetase but lacking the anticodon-binding domain. We show that YadB is a tRNA modifying...
Dubois, Daniel Y., Blaise, Mickaël, Becker, Hubert D., Campanacci, Valérie, Keith, Gérard, Giegé, Richard, ...
The product of the Escherichia coli yadB gene is homologous to the N-terminal part of bacterial glutamyl-tRNA synthetases (GluRSs), including the Rossmann fold with the acceptor-binding domain and...
Isolation of Llama Antibody Fragments for Prevention of Dandruff by Phage Display in Shampoo
Dolk, Edward, Van Der Vaart, Marcel, Lutje Hulsik, David, Vriend, Gert, De Haard, Hans, Spinelli, Silvia, ...
As part of research exploring the feasibility of using antibody fragments to inhibit the growth of organisms implicated in dandruff, we isolated antibody fragments that bind to a cell surface protein...
Control of domain swapping in bovine odorant-binding protein.
Ramoni, Roberto, Vincent, Florence, Ashcroft, Alison E, Accornero, Paolo, Grolli, Stefano, Valencia, Christel, ...
As revealed by the X-ray structure, bovine odorant-binding protein (OBPb) is a domain swapped dimer [Tegoni, Ramoni, Bignetti, Spinelli and Cambillau (1996) Nat. Struct. Biol. 3, 863-867; Bianchet,...
Ferrat, Gilles, Renisio, Jean-Guillaume, Morelli, Xavier, Slootstra, Jerry, Meloen, Rob, Cambillau, Christian, ...
The X-ray structure of a ternary complex between human chorionic gonadotropin hormone (hCG) and two Fvs recognizing its alpha and beta subunits has been recently determined. The Fvs recognize the...
Solution structure of a chemosensory protein from the moth Mamestra brassicae.
Mosbah, Amor, Campanacci, Valérie, Lartigue, Audrey, Tegoni, Mariella, Cambillau, Christian, Darbon, Hervé
Chemosensory proteins (CSPs) are believed to be involved in chemical communication and perception. A number of such proteins, of molecular mass approximately 13 kDa, have been isolated from different...
Crystal structure of nitrous oxide reductase from Paracoccus denitrificans at 1.6 A resolution.
Haltia, Tuomas, Brown, Kieron, Tegoni, Mariella, Cambillau, Christian, Saraste, Matti, Mattila, Kimmo, ...
N2O is generated by denitrifying bacteria as a product of NO reduction. In denitrification, N2O is metabolized further by the enzyme N2O reductase (N2OR), a multicopper protein which converts N2O...
Rivière, Stéphane, Lartigue, Audrey, Quennedey, Brigitte, Campanacci, Valérie, Farine, Jean-Pierre, Tegoni, Mariella, ...
Odorant-binding proteins (OBPs) are thought to transport volatile compounds from air to their receptors through the sensillary lymph. In this protein family, the subgroup of pheromone-binding...
Tremblay, Denise M., Tegoni, Mariella, Spinelli, Silvia, Campanacci, Valérie, Blangy, Stéphanie, Huyghe, Céline, ...
Phage p2, a member of the lactococcal 936 phage species, infects Lactococcus lactis strains by binding initially to specific carbohydrate receptors using its receptor-binding protein (RBP). The...
Minskaia, Ekaterina, Hertzig, Tobias, Gorbalenya, Alexander E., Campanacci, Valérie, Cambillau, Christian, Canard, Bruno, ...
Replication of the giant RNA genome of severe acute respiratory syndrome (SARS) coronavirus (CoV) and synthesis of as many as eight subgenomic (sg) mRNAs are mediated by a viral...
Structural and Functional Basis for ADP-Ribose and Poly(ADP-Ribose) Binding by Viral Macro Domains
Egloff, Marie-Pierre, Malet, Hélène, Putics, Ákos, Heinonen, Maarit, Dutartre, Hélène, Frangeul, Antoine, ...
Macro domains constitute a protein module family found associated with specific histones and proteins involved in chromatin metabolism. In addition, a small number of animal RNA viruses, such as...
Crystal Structure of the Receptor-Binding Protein Head Domain from Lactococcus lactis Phage bIL170
Ricagno, Stefano, Campanacci, Valérie, Blangy, Stéphanie, Spinelli, Silvia, Tremblay, Denise, Moineau, Sylvain, ...
Lactococcus lactis, a gram-positive bacterium widely used by the dairy industry, is subject to lytic phage infections. In the first step of infection, phages recognize the host saccharidic receptor...
Crystal structure of AFV3-109, a highly conserved protein from crenarchaeal viruses
Keller, Jenny, Leulliot, Nicolas, Cambillau, Christian, Campanacci, Valérie, Porciero, Stéphanie, Prangishvilli, David, ...
The extraordinary morphologies of viruses infecting hyperthermophilic archaea clearly distinguish them from bacterial and eukaryotic viruses. Moreover, their genomes code for proteins that to a large...
Sulzenbacher, Gerlind, Canaan, Stéphane, Bordat, Yann, Neyrolles, Olivier, Stadthagen, Gustavo, Roig-Zamboni, Véronique, ...
Cell envelope lipids play an important role in the pathogenicity of mycobacteria, but the mechanisms by which they are transported to the outer membrane of these prokaryotes are largely unknown....
Ricagno, Stefano, Egloff, Marie-Pierre, Ulferts, Rachel, Coutard, Bruno, Nurizzo, Didier, Campanacci, Valérie, ...
The ≈30-kb coronavirus (+)RNA genome is replicated and transcribed by a membrane-bound replicase complex made up of 16 viral nonstructural proteins (nsp) with multiple enzymatic activities. The...
Expression, purification and crystallization of the SARS-CoV macro domain
Malet, Hélène, Dalle, Karen, Brémond, Nicolas, Tocque, Fabienne, Blangy, Stéphanie, Campanacci, Valérie, ...
The SARS-CoV macro domain was expressed, purified and crystallized. Selenomethionine-labelled crystals diffracted to 1.8 Å resolution.
Crystallization and preliminary X-ray diffraction analysis of Nsp15 from SARS coronavirus
Ricagno, Stéfano, Coutard, Bruno, Grisel, Sacha, Brémond, Nicolas, Dalle, Karen, Tocque, Fabienne, ...
Crystals of Nsp15 from the aetiological agent of SARS have been grown at room temperature. Crystals have cubic symmetry and diffract to a maximum resolution of 2.7 Å.
Honegger, Annemarie, Spinelli, Silvia, Cambillau, Christian, Plückthun, Andreas
The structure of the scFv fragment FITC-E2, obtained from a naive phage antibody scFv library derived from human donors, was determined at 2.1 Å resolution in the free form and at 3.0 Å in the...
High-throughput automated refolding screening of inclusion bodies
Vincentelli, Renaud, Canaan, Stéphane, Campanacci, Valérie, Valencia, Christel, Maurin, Damien, Frassinetti, Frédéric, ...
One of the main stumbling blocks encountered when attempting to express foreign proteins in Escherichia coli is the occurrence of amorphous aggregates of misfolded proteins, called inclusion bodies...
Toyota, Cory G., Berthold, Catrine L., Gruez, Arnaud, Jónsson, Stefán, Lindqvist, Ylva, Cambillau, Christian, ...
The yfdXWUVE operon appears to encode proteins that enhance the ability of Escherichia coli MG1655 to survive under acidic conditions. Although the molecular mechanisms underlying this phenotypic...
Goulet, Adeline, Spinelli, Silvia, Campanacci, Valérie, Porciero, Sophie, Blangy, Stéphanie, Garrett, Roger A., ...
Crystals of S. islandicus filamentous virus (SIFV) protein 14 have been grown at 293 K. Crystals belong to space group P6222 or P6422 and diffract to a resolution of 2.95 Å.
Improved crystallization of the coxsackievirus B3 RNA-dependent RNA polymerase
Jabafi, Ilham, Selisko, Barbara, Coutard, Bruno, De Palma, Armando M., Neyts, Johan, Egloff, Marie-Pierre, ...
The first crystal of a coxsackievirus RNA-dependent RNA polymerase is reported.
Siponen, Marina, Sciara, Giuliano, Villion, Manuela, Spinelli, Silvia, Lichière, Julie, Cambillau, Christian, ...
We report here the characterization of the nonstructural protein ORF12 of the virulent lactococcal phage p2, which belongs to the Siphoviridae family. ORF12 was produced as a soluble protein, which...
Crystal Structure of a Chimeric Receptor Binding Protein Constructed from Two Lactococcal Phages▿ †
Siponen, Marina, Spinelli, Silvia, Blangy, Stéphanie, Moineau, Sylvain, Cambillau, Christian, Campanacci, Valérie
Lactococcus lactis, a gram-positive bacterium widely used by the dairy industry to manufacture cheeses, is subject to infection by a diverse population of virulent phages. We have previously...