Christian Schley, Matthias Altmeyer, Rolf Müller, Christian G. Huber
Abstract. In order to generate proteomics data that are suitable to validate protein identification in complex mixtures using multidimensional liquidchromatography-mass spectrometry approaches, we...
Hansjörg Toll, Peter Berger, Andreas Hofmann, Andreas Hildebr, Hans Peter Lenhof, Christian G. Huber
Abstract. Due to their extensive structural heterogeneity, the elucidation of glycosylation patterns in glycoproteins such as the subunits of human chorionic gonadotropin (hCG), hCG-α and hCG-β �...
Pfeifer, Nico, Leinenbach, Andreas, Huber, Christian G, Kohlbacher, Oliver
Abstract Background High-throughput peptide and protein identification technologies have benefited tremendously from strategies based on tandem mass spectrometry (MS/MS) in combination with database...
Sturm, Marc, Quinten, Sascha, Huber, Christian G., Kohlbacher, Oliver
We propose a new model for predicting the retention time of oligonucleotides. The model is based on ν support vector regression using features derived from base sequence and predicted secondary...
TNF-alpha-Wirkungen auf die Enterochromaffin-like Zellen des Magens (2006)
Enterochromaffin-like (ECL) Zellen sind als histaminhaltige Zellen des Magenepithels für die periphere Regulation der Magensäuresekretion verantwortlich. Bei chronischer Gastritis sind sie...
Yoo, Chul, Zhao, Jia, Pal, Manoj, Hersberger, Katherine, Huber, Christian G., Simeone, Diane M., ...
A unique approach of automating the integration of monolithic capillary HPLC-based protein separation and on-plate digestion for subsequent MALDI-MS analysis has been developed. All liquid-handling...
Schley, Christian, Altmeyer, Matthias, Müller, Rolf, Huber, Christian G.
In order to generate proteomics data that are suitable to validate protein identification in complex mixtures using multidimensional liquid-chromatography-mass spectrometry approaches, we implemented...
05471 Executive Summary -- Computational Proteomics (2006)
Huber, Christian G., Kohlbacher, Oliver, Reinert, Knut
The Dagstuhl Seminar on Computational Proteomics brought together researchers from computer science and from proteomics to discuss the state of the art and future developments at the interface...
Evaluation of LC-MS data for the absolute quantitative analysis of marker proteins (2006)
Delmotte, Nathanaël, Mayr, Bettina, Leinenbach, Andreas, Reinert, Knut, Kohlbacher, Oliver, Klein, Christoph, ...
The serum complexity makes the absolute quantitative analysis of medium to low-abundant proteins very challenging. Tens of thousands proteins are present in human serum and dispersed over an...
Toll, Hansjörg, Berger, Peter, Hofmann, Andreas, Hildebrandt, Andreas, Oberacher, Herbert, Lenhof, Hans Peter, ...
Due to their extensive structural heterogeneity, the elucidation of glycosylation patterns in glycoproteins such as the subunits of chorionic gonadotropin (CG), CG-alpha and CG-beta remains one of...
05471 Abstract Collection -- Computational Proteomics (2006)
Huber, Christian G., Kohlbacher, Oliver, Reinert, Knut
From 20.11.05 to 25.11.05, the Dagstuhl Seminar 05471 ``Computational Proteomics'' was held in the International Conference and Research Center (IBFI), Schloss Dagstuhl. During the seminar, several...
A machine learning approach for prediction of DNA and peptide HPLC retention times (2006)
Sturm, Marc, Quinten, Sascha, Huber, Christian G., Kohlbacher, Oliver
High performance liquid chromatography (HPLC) has become one of the most efficient methods for the separation of biomolecules. It is an important tool in DNA purification after synthesis as well as...
Hoelzl, Georg, Oberacher, Herbert, Pitsch, Stefan, Stutz, Alfred, Huber, Christian G.
Ion-pair reversed-phase high-performance liq. chromatog. (IP-RP-HPLC) has been evaluated as a method for the fractionation and desalting of ribonucleic acids prior to their characterization by...
Temperature-Modulated Array High-Performance Liquid Chromatography (2001)
Premstaller, Andreas, Xiao, Wenzhong, Oberacher, Herbert, O'Keefe, Matthew, Stern, David, Willis, Thomas, ...
Huber, Christian G., Oefner, Peter J., Preuss, Eugen, Bonn, Günther K.
DNA restriction fragments and PCR products were separated by means of ion-pair reversed-phase high-performance liquid chromatography on alkylated non-porous poly(styrene-divinylbenzene) particles...
Zolla, Lello, Rinalducci, Sara, Timperio, Anna Maria, Huber, Christian G.
The light-harvesting proteins (Lhca) of photosystem I (PSI) from four monocot and five dicot species were extracted from plant material, separated by reversed-phase high-performance liquid...
Zolla, Lello, Timperio, Anna-Maria, Walcher, Wolfgang, Huber, Christian G.
An overview of the intact molecular masses and the hydrophobic properties of the photosystem II (PSII) light-harvesting proteins in 14 different plant species is presented. The protein separation and...
Temperature-Modulated Array High-Performance Liquid Chromatography
Premstaller, Andreas, Xiao, Wenzhong, Oberacher, Herbert, O'Keefe, Matthew, Stern, David, Willis, Thomas, ...
Using novel monolithic poly(styrene-divinylbenzene) capillary columns with an internal diameter of 0.2 mm, we demonstrate for the first time the feasibility of constructing high-performance liquid...
Zolla, Lello, Rinalducci, Sara, Timperio, Anna Maria, Huber, Christian G.
The light-harvesting proteins (Lhca) of photosystem I (PSI) from four monocot and five dicot species were extracted from plant material, separated by reversed-phase high-performance liquid...
Zolla, Lello, Timperio, Anna-Maria, Walcher, Wolfgang, Huber, Christian G.
An overview of the intact molecular masses and the hydrophobic properties of the photosystem II (PSII) light-harvesting proteins in 14 different plant species is presented. The protein separation and...
Temperature-Modulated Array High-Performance Liquid Chromatography
Premstaller, Andreas, Xiao, Wenzhong, Oberacher, Herbert, O'Keefe, Matthew, Stern, David, Willis, Thomas, ...
Using novel monolithic poly(styrene-divinylbenzene) capillary columns with an internal diameter of 0.2 mm, we demonstrate for the first time the feasibility of constructing high-performance liquid...
Sturm, Marc, Quinten, Sascha, Huber, Christian G., Kohlbacher, Oliver
We propose a new model for predicting the retention time of oligonucleotides. The model is based on ν support vector regression using features derived from base sequence and predicted secondary...
Yoo, Chul, Patwa, Tasneem H., Kreunin, Paweena, Miller, Fred R., Huber, Christian G., Nesvizhskii, Alexey I., ...
A comprehensive platform that integrates information from the protein and peptide levels by combining various MS techniques has been employed for the analysis of proteins in fully malignant human...