Christian Vadeboncoeur

The relevance of genetic analysis to dairy bacteria: building upon our heritage (2004)

Vadeboncoeur, Christian, Moineau, Sylvain

Abstract Lactic acid bacteria (LAB) are essential for the manufacture of fermented dairy products. Studies on the physiology, biochemistry and genetics of these microorganisms over the last century...

The HPr(Ser) Kinase of Streptococcus salivarius: Purification, Properties, and Cloning of the hprK Gene

Brochu, Denis, Vadeboncoeur, Christian

In gram-positive bacteria, HPr, a protein of the phosphoenolpyruvate:sugar phosphotransferase system, is phosphorylated on a serine residue at position 46 by an ATP-dependent protein kinase. The...

Phenotypic Consequences Resulting from a Methionine-to-Valine Substitution at Position 48 in the HPr Protein of Streptococcus salivarius

Plamondon, Pascale, Brochu, Denis, Thomas, Suzanne, Fradette, Julie, Gauthier, Lucie, Vaillancourt, Katy, ...

In gram-positive bacteria, the HPr protein of the phosphoenolpyruvate:sugar phosphotransferase system (PTS) can be phosphorylated on a histidine residue at position 15 (His15) by enzyme I (EI) of the...

Streptococcus salivarius Fimbriae Are Composed of a Glycoprotein Containing a Repeated Motif Assembled into a Filamentous Nondissociable Structure

Lévesque, Céline, Vadeboncoeur, Christian, Chandad, Fatiha, Frenette, Michel

Streptococcus salivarius, a gram-positive bacterium found in the human oral cavity, expresses flexible peritrichous fimbriae. In this paper, we report purification and partial characterization of S....

An Effective Strategy, Applicable to Streptococcus salivarius and Related Bacteria, To Enhance or Confer Electroporation Competence

Buckley, Nicole D., Vadeboncoeur, Christian, LeBlanc, Donald J., Lee, Linda N., Frenette, Michel

Despite the large number of techniques available for transformation of bacteria, certain species and strains are still resistant to introduction of foreign DNA. Some oral streptococci are among the...

Novel Food-Grade Plasmid Vector Based on Melibiose Fermentation for the Genetic Engineering of Lactococcus lactis

Boucher, Isabelle, Parrot, Marc, Gaudreau, Hélène, Champagne, Claude P., Vadeboncoeur, Christian, Moineau, Sylvain

The α-galactosidase gene (aga) and a gene coding for a putative transcriptional regulator from the LacI/GalR family (galR) of Lactococcus raffinolactis ATCC 43920 were cloned and sequenced. When...

Characterization of Genes Involved in the Metabolism of α-Galactosides by Lactococcus raffinolactis

Boucher, Isabelle, Vadeboncoeur, Christian, Moineau, Sylvain

Lactococcus raffinolactis, unlike most lactococci, is able to ferment α-galactosides, such as melibiose and raffinose. More than 12 kb of chromosomal DNA from L. raffinolactis ATCC 43920 was...

Genetic and Biochemical Characterization of the Phosphoenolpyruvate:Glucose/Mannose Phosphotransferase System of Streptococcus thermophilus

Cochu, Armelle, Vadeboncoeur, Christian, Moineau, Sylvain, Frenette, Michel

In most streptococci, glucose is transported by the phosphoenolpyruvate (PEP):glucose/mannose phosphotransferase system (PTS) via HPr and IIABMan, two proteins involved in regulatory mechanisms....

Phosphorylation of Streptococcus salivarius Lactose Permease (LacS) by HPr(His∼P) and HPr(Ser-P)(His∼P) and Effects on Growth

Lessard, Christian, Cochu, Armelle, Lemay, Jean-Dominique, Roy, Denis, Vaillancourt, Katy, Frenette, Michel, ...

The oral bacterium Streptococcus salivarius takes up lactose via a transporter called LacS that shares 95% identity with the LacS from Streptococcus thermophilus, a phylogenetically closely related...

Comparative Study of Streptococcus mutans Laboratory Strains and Fresh Isolates from Carious and Caries-Free Tooth Surfaces and from Subjects with Hereditary Fructose Intolerance

Vadeboncoeur, Christian, Trahan, Luc

This study was undertaken to investigate and compare some biochemical and physiological properties related to sugar metabolism of 4 laboratory strains and 13 freshly isolated strains of Streptococcus...

Characterization of a Galactokinase-Positive Recombinant Strain of Streptococcus thermophilus

Vaillancourt, Katy, LeMay, Jean-Dominique, Lamoureux, Maryse, Frenette, Michel, Moineau, Sylvain, Vadeboncoeur, Christian

The lactic acid bacterium Streptococcus thermophilus is widely used by the dairy industry for its ability to transform lactose, the primary sugar found in milk, into lactic acid. Unlike the...

The relevance of genetic analysis to dairy bacteria: building upon our heritage

Vadeboncoeur, Christian, Moineau, Sylvain

Lactic acid bacteria (LAB) are essential for the manufacture of fermented dairy products. Studies on the physiology, biochemistry and genetics of these microorganisms over the last century have...

The Doubly Phosphorylated Form of HPr, HPr(Ser-P)(His∼P), Is Abundant in Exponentially Growing Cells of Streptococcus thermophilus and Phosphorylates the Lactose Transporter LacS as Efficiently as HPr(His∼P)

Cochu, Armelle, Roy, Denis, Vaillancourt, Katy, LeMay, Jean-Dominique, Casabon, Israël, Frenette, Michel, ...

In Streptococcus thermophilus, lactose is taken up by LacS, a transporter that comprises a membrane translocator domain and a hydrophilic regulatory domain homologous to the IIA proteins and protein...

Characterization of the cro-ori Region of the Streptococcus thermophilus Virulent Bacteriophage DT1

Lamothe, Geneviève, Lévesque, Céline, Bissonnette, Frédéric, Cochu, Armelle, Vadeboncoeur, Christian, Frenette, Michel, ...

The virulent cos-type Streptococcus thermophilus phage DT1 was previously isolated from a mozzarella whey sample, and its complete genomic sequence is available. The putative ori of phage DT1 is...

The HPr(Ser) Kinase of Streptococcus salivarius: Purification, Properties, and Cloning of the hprK Gene

Brochu, Denis, Vadeboncoeur, Christian

In gram-positive bacteria, HPr, a protein of the phosphoenolpyruvate:sugar phosphotransferase system, is phosphorylated on a serine residue at position 46 by an ATP-dependent protein kinase. The...

Phenotypic Consequences Resulting from a Methionine-to-Valine Substitution at Position 48 in the HPr Protein of Streptococcus salivarius

Plamondon, Pascale, Brochu, Denis, Thomas, Suzanne, Fradette, Julie, Gauthier, Lucie, Vaillancourt, Katy, ...

In gram-positive bacteria, the HPr protein of the phosphoenolpyruvate:sugar phosphotransferase system (PTS) can be phosphorylated on a histidine residue at position 15 (His15) by enzyme I (EI) of the...

Streptococcus salivarius Fimbriae Are Composed of a Glycoprotein Containing a Repeated Motif Assembled into a Filamentous Nondissociable Structure

Lévesque, Céline, Vadeboncoeur, Christian, Chandad, Fatiha, Frenette, Michel

Streptococcus salivarius, a gram-positive bacterium found in the human oral cavity, expresses flexible peritrichous fimbriae. In this paper, we report purification and partial characterization of S....

An Effective Strategy, Applicable to Streptococcus salivarius and Related Bacteria, To Enhance or Confer Electroporation Competence

Buckley, Nicole D., Vadeboncoeur, Christian, LeBlanc, Donald J., Lee, Linda N., Frenette, Michel

Despite the large number of techniques available for transformation of bacteria, certain species and strains are still resistant to introduction of foreign DNA. Some oral streptococci are among the...

Novel Food-Grade Plasmid Vector Based on Melibiose Fermentation for the Genetic Engineering of Lactococcus lactis

Boucher, Isabelle, Parrot, Marc, Gaudreau, Hélène, Champagne, Claude P., Vadeboncoeur, Christian, Moineau, Sylvain

The α-galactosidase gene (aga) and a gene coding for a putative transcriptional regulator from the LacI/GalR family (galR) of Lactococcus raffinolactis ATCC 43920 were cloned and sequenced. When...

Characterization of Genes Involved in the Metabolism of α-Galactosides by Lactococcus raffinolactis

Boucher, Isabelle, Vadeboncoeur, Christian, Moineau, Sylvain

Lactococcus raffinolactis, unlike most lactococci, is able to ferment α-galactosides, such as melibiose and raffinose. More than 12 kb of chromosomal DNA from L. raffinolactis ATCC 43920 was...

Genetic and Biochemical Characterization of the Phosphoenolpyruvate:Glucose/Mannose Phosphotransferase System of Streptococcus thermophilus

Cochu, Armelle, Vadeboncoeur, Christian, Moineau, Sylvain, Frenette, Michel

In most streptococci, glucose is transported by the phosphoenolpyruvate (PEP):glucose/mannose phosphotransferase system (PTS) via HPr and IIABMan, two proteins involved in regulatory mechanisms....

Phosphorylation of Streptococcus salivarius Lactose Permease (LacS) by HPr(His∼P) and HPr(Ser-P)(His∼P) and Effects on Growth

Lessard, Christian, Cochu, Armelle, Lemay, Jean-Dominique, Roy, Denis, Vaillancourt, Katy, Frenette, Michel, ...

The oral bacterium Streptococcus salivarius takes up lactose via a transporter called LacS that shares 95% identity with the LacS from Streptococcus thermophilus, a phylogenetically closely related...

Comparative Study of Streptococcus mutans Laboratory Strains and Fresh Isolates from Carious and Caries-Free Tooth Surfaces and from Subjects with Hereditary Fructose Intolerance

Vadeboncoeur, Christian, Trahan, Luc

This study was undertaken to investigate and compare some biochemical and physiological properties related to sugar metabolism of 4 laboratory strains and 13 freshly isolated strains of Streptococcus...

Characterization of a Galactokinase-Positive Recombinant Strain of Streptococcus thermophilus

Vaillancourt, Katy, LeMay, Jean-Dominique, Lamoureux, Maryse, Frenette, Michel, Moineau, Sylvain, Vadeboncoeur, Christian

The lactic acid bacterium Streptococcus thermophilus is widely used by the dairy industry for its ability to transform lactose, the primary sugar found in milk, into lactic acid. Unlike the...

The relevance of genetic analysis to dairy bacteria: building upon our heritage

Vadeboncoeur, Christian, Moineau, Sylvain

Lactic acid bacteria (LAB) are essential for the manufacture of fermented dairy products. Studies on the physiology, biochemistry and genetics of these microorganisms over the last century have...

The Doubly Phosphorylated Form of HPr, HPr(Ser-P)(His∼P), Is Abundant in Exponentially Growing Cells of Streptococcus thermophilus and Phosphorylates the Lactose Transporter LacS as Efficiently as HPr(His∼P)

Cochu, Armelle, Roy, Denis, Vaillancourt, Katy, LeMay, Jean-Dominique, Casabon, Israël, Frenette, Michel, ...

In Streptococcus thermophilus, lactose is taken up by LacS, a transporter that comprises a membrane translocator domain and a hydrophilic regulatory domain homologous to the IIA proteins and protein...

Characterization of the cro-ori Region of the Streptococcus thermophilus Virulent Bacteriophage DT1

Lamothe, Geneviève, Lévesque, Céline, Bissonnette, Frédéric, Cochu, Armelle, Vadeboncoeur, Christian, Frenette, Michel, ...

The virulent cos-type Streptococcus thermophilus phage DT1 was previously isolated from a mozzarella whey sample, and its complete genomic sequence is available. The putative ori of phage DT1 is...

Role of galK and galM in Galactose Metabolism by Streptococcus thermophilus▿

Vaillancourt, Katy, Bédard, Nathalie, Bart, Christian, Tessier, Mélanie, Robitaille, Gilles, Turgeon, Nathalie, ...

Streptococcus thermophilus is unable to metabolize the galactose moiety of lactose. In this paper, we show that a transformant of S. thermophilus SMQ-301 expressing Streptococcus salivarius galK and...