Coumoul, Xavier, Li, Wenmei, Wang, Rui-Hong, Deng, Chuxia
RNA interference (RNAi) is a simple and powerful tool widely used for studying gene function in a number of species. Recently, inducible regulation of RNAi in mammalian cells using either...
Chen, Lin, Li, Cuiling, Qiao, Wenhui, Xu, Xiaoling, Deng, Chuxia
Missense mutations in fibroblast growth factor receptor 3 (FGFR3) result in several types of human skeletal dysplasia, including the neonatally lethal dwarfism known as thanatophoric dysplasia. An...
Location of crossovers during gene targeting with insertion and replacement vectors. (1993)
Gene targeting was used to introduce nonselectable genetic changes into chromosomal loci in mouse embryo-derived stem cells. The nonselectable markers were linked to a selectable marker in both...
Location of crossovers during gene targeting with insertion and replacement vectors. (1993)
Gene targeting was used to introduce nonselectable genetic changes into chromosomal loci in mouse embryo-derived stem cells. The nonselectable markers were linked to a selectable marker in both...
Location of crossovers during gene targeting with insertion and replacement vectors. (1993)
Gene targeting was used to introduce nonselectable genetic changes into chromosomal loci in mouse embryo-derived stem cells. The nonselectable markers were linked to a selectable marker in both...
Capecchi, Mario R.;, Deng, Chuxia ;
Mutations were targeted to the Hprt locus of mouse embryo-derived stem cells by using 22 different sequence replacement and sequence insertion vectors. The targeting frequency was examined at two...
Capecchi, Mario R.;, Deng, Chuxia ;
Mutations were targeted to the Hprt locus of mouse embryo-derived stem cells by using 22 different sequence replacement and sequence insertion vectors. The targeting frequency was examined at two...
High-fidelity gene targeting in embryonic stem cells by using sequence replacement vectors. (1992)
Mutations were targeted to the Hprt locus in murine embryonic stem cells by using sequence replacement vectors. When the vector was designed such that the mutated sequences were flanked on both sides...
High-fidelity gene targeting in embryonic stem cells by using sequence replacement vectors. (1992)
Mutations were targeted to the Hprt locus in murine embryonic stem cells by using sequence replacement vectors. When the vector was designed such that the mutated sequences were flanked on both sides...
High-fidelity gene targeting in embryonic stem cells by using sequence replacement vectors. (1992)
Mutations were targeted to the Hprt locus in murine embryonic stem cells by using sequence replacement vectors. When the vector was designed such that the mutated sequences were flanked on both sides...
Thesis (Ph. D.)--Dept. of Biology, University of Utah, 1992.
Introduction of a lacZ reporter gene into the mouse int-2 locus by homologous recombination. (1990)
We demonstrate that the frequency of gene targeting is unaffected by the length of nonhomologous DNA transferred to a target chromosomal sequence. A result of this finding is that a much wider...
Introduction of a lacZ reporter gene into the mouse int-2 locus by homologous recombination. (1990)
We demonstrate that the frequency of gene targeting is unaffected by the length of nonhomologous DNA transferred to a target chromosomal sequence. A result of this finding is that a much wider...
Introduction of a lacZ reporter gene into the mouse int-2 locus by homologous recombination. (1990)
We demonstrate that the frequency of gene targeting is unaffected by the length of nonhomologous DNA transferred to a target chromosomal sequence. A result of this finding is that a much wider...
A vital role for glycosphingolipid synthesis during development and differentiation
Yamashita, Tadashi, Wada, Ryuichi, Sasaki, Teiji, Deng, Chuxia, Bierfreund, Uwe, Sandhoff, Konrad, ...
Glycosphingolipids (GSLs) are believed to be integral for the dynamics of many cell membrane events, including cellular interactions, signaling, and trafficking. We have investigated their roles in...
Xu, Jianming, Liao, Lan, Ning, Guang, Yoshida-Komiya, Hiromi, Deng, Chuxia, O'Malley, Bert W.
Steroid receptor coactivator-3 (SRC-3) is a coactivator of nuclear receptors in the SRC family as assayed in vitro. Here, we show that mouse SRC-3 is expressed in a tissue-specific fashion and...
Yang, Xiao, Li, Cuiling, Xu, Xiaoling, Deng, Chuxia
Members of the transforming growth factor (TGF)-β superfamily have been shown to play a variety of important roles in embryogenesis, including dorsal and ventral mesoderm induction. The tumor...
Franklin, David S., Godfrey, Virginia L., O'Brien, Deborah A., Deng, Chuxia, Xiong, Yue
The presence of two families of seven distinct mammalian cyclin-dependent kinase (CDK) inhibitor genes is thought to mediate the complexity of connecting a variety of cellular processes to the cell...
Defective lysosomal targeting of activated fibroblast growth factor receptor 3 in achondroplasia
Cho, Jay Y., Guo, Changsheng, Torello, Monica, Lunstrum, Gregory P., Iwata, Tomoko, Deng, Chuxia, ...
Mutations of fibroblast growth factor receptor 3 (FGFR3) are responsible for achondroplasia (ACH) and related dwarfing conditions in humans. The pathogenesis involves constitutive activation of...
A genetic model of substrate deprivation therapy for a glycosphingolipid storage disorder
Liu, Yujing, Wada, Ryuichi, Kawai, Hiromichi, Sango, Kazunori, Deng, Chuxia, Tai, Tadashi, ...
Inherited defects in the degradation of glycosphingolipids (GSLs) cause a group of severe diseases known as GSL storage disorders. There are currently no effective treatments for the majority of...
Coumoul, Xavier, Li, Wenmei, Wang, Rui-Hong, Deng, Chuxia
RNA interference (RNAi) is a simple and powerful tool widely used for studying gene function in a number of species. Recently, inducible regulation of RNAi in mammalian cells using either...
Castrop, Hayo, Huang, Yuning, Hashimoto, Seiji, Mizel, Diane, Hansen, Pernille, Theilig, Franziska, ...
Adenosine coordinates organ metabolism and blood supply, and it modulates immune responses. In the kidney it mediates the vascular response elicited by changes in NaCl concentration in the macula...
Liu, Jie, Chen, Min, Deng, Chuxia, Bourc'his, Déborah, Nealon, Julie G., Erlichman, Beth, ...
Gnas is a complex gene with multiple imprinted promoters. The upstream Nesp and Nespas/Gnasxl promoters are paternally and maternally methylated, respectively. The downstream promoter for the...
Alternative Gnas gene products have opposite effects on glucose and lipid metabolism
Chen, Min, Gavrilova, Oksana, Liu, Jie, Xie, Tao, Deng, Chuxia, Nguyen, Annie T., ...
Gnas is an imprinted gene with multiple gene products resulting from alternative splicing of different first exons onto a common exon 2. These products include stimulatory G protein α-subunit...
Essential Role of Smad3 in Angiotensin II–Induced Vascular Fibrosis
Wang, Wansheng, Huang, Xiao R., Canlas, Ellery, Oka, Kazuhiro, Truong, Luan D., Deng, Chuxia, ...
Angiotensin II (Ang II) plays a pivotal role in vascular fibrosis, which leads to serious complications in hypertension and diabetes. However, the underlying signaling mechanisms are largely unclear....
A vital role for glycosphingolipid synthesis during development and differentiation
Yamashita, Tadashi, Wada, Ryuichi, Sasaki, Teiji, Deng, Chuxia, Bierfreund, Uwe, Sandhoff, Konrad, ...
Glycosphingolipids (GSLs) are believed to be integral for the dynamics of many cell membrane events, including cellular interactions, signaling, and trafficking. We have investigated their roles in...
Xu, Jianming, Liao, Lan, Ning, Guang, Yoshida-Komiya, Hiromi, Deng, Chuxia, O'Malley, Bert W.
Steroid receptor coactivator-3 (SRC-3) is a coactivator of nuclear receptors in the SRC family as assayed in vitro. Here, we show that mouse SRC-3 is expressed in a tissue-specific fashion and...
Yang, Xiao, Li, Cuiling, Xu, Xiaoling, Deng, Chuxia
Members of the transforming growth factor (TGF)-β superfamily have been shown to play a variety of important roles in embryogenesis, including dorsal and ventral mesoderm induction. The tumor...
Franklin, David S., Godfrey, Virginia L., O'Brien, Deborah A., Deng, Chuxia, Xiong, Yue
The presence of two families of seven distinct mammalian cyclin-dependent kinase (CDK) inhibitor genes is thought to mediate the complexity of connecting a variety of cellular processes to the cell...
Defective lysosomal targeting of activated fibroblast growth factor receptor 3 in achondroplasia
Cho, Jay Y., Guo, Changsheng, Torello, Monica, Lunstrum, Gregory P., Iwata, Tomoko, Deng, Chuxia, ...
Mutations of fibroblast growth factor receptor 3 (FGFR3) are responsible for achondroplasia (ACH) and related dwarfing conditions in humans. The pathogenesis involves constitutive activation of...
A genetic model of substrate deprivation therapy for a glycosphingolipid storage disorder
Liu, Yujing, Wada, Ryuichi, Kawai, Hiromichi, Sango, Kazunori, Deng, Chuxia, Tai, Tadashi, ...
Inherited defects in the degradation of glycosphingolipids (GSLs) cause a group of severe diseases known as GSL storage disorders. There are currently no effective treatments for the majority of...
Coumoul, Xavier, Li, Wenmei, Wang, Rui-Hong, Deng, Chuxia
RNA interference (RNAi) is a simple and powerful tool widely used for studying gene function in a number of species. Recently, inducible regulation of RNAi in mammalian cells using either...
Castrop, Hayo, Huang, Yuning, Hashimoto, Seiji, Mizel, Diane, Hansen, Pernille, Theilig, Franziska, ...
Adenosine coordinates organ metabolism and blood supply, and it modulates immune responses. In the kidney it mediates the vascular response elicited by changes in NaCl concentration in the macula...
Liu, Jie, Chen, Min, Deng, Chuxia, Bourc'his, Déborah, Nealon, Julie G., Erlichman, Beth, ...
Gnas is a complex gene with multiple imprinted promoters. The upstream Nesp and Nespas/Gnasxl promoters are paternally and maternally methylated, respectively. The downstream promoter for the...
Alternative Gnas gene products have opposite effects on glucose and lipid metabolism
Chen, Min, Gavrilova, Oksana, Liu, Jie, Xie, Tao, Deng, Chuxia, Nguyen, Annie T., ...
Gnas is an imprinted gene with multiple gene products resulting from alternative splicing of different first exons onto a common exon 2. These products include stimulatory G protein α-subunit...
Ewing sarcoma gene EWS is essential for meiosis and B lymphocyte development
Li, Hongjie, Watford, Wendy, Li, Cuiling, Parmelee, Alissa, Bryant, Mark A., Deng, Chuxia, ...
Ewing sarcoma gene EWS encodes a putative RNA-binding protein with proposed roles in transcription and splicing, but its physiological role in vivo remains undefined. Here, we have generated...
Mice Lacking Smad3 Are Protected Against Cutaneous Injury Induced by Ionizing Radiation
Flanders, Kathleen C., Sullivan, Catherine D., Fujii, Makiko, Sowers, Anastasia, Anzano, Mario A., Arabshahi, Alidad, ...
Transforming growth factor-β (TGF-β) plays a central role in the pathogenesis of inflammatory and fibrotic diseases, including radiation-induced fibrosis. We previously reported that mice null for...
Peripheral Disruption of the Grb10 Gene Enhances Insulin Signaling and Sensitivity In Vivo▿
Wang, Lixin, Balas, Bogdan, Christ-Roberts, Christine Y., Kim, Ryang Yeo, Ramos, Fresnida J., Kikani, Chintan K., ...
Grb10 is a pleckstrin homology and Src homology 2 domain-containing protein that interacts with a number of phosphorylated receptor tyrosine kinases, including the insulin receptor. In mice, Grb10...
Lee, H. Thomas, Chen, Sean W. C., Doetschman, Thomas C., Deng, Chuxia, D'Agati, Vivette D., Kim, Mihwa
We previously demonstrated that several clinically utilized volatile anesthetics including sevoflurane protected against renal ischemia-reperfusion (IR) injury by reducing necrosis and inflammation...
Bornstein, Sophia, White, Ruth, Malkoski, Stephen, Oka, Masako, Han, Gangwen, Cleaver, Timothy, ...
Smad4 is a central mediator of TGF-β signaling, and its expression is downregulated or lost at the malignant stage in several cancer types. In this study, we found that Smad4 was frequently...