Krishnan, B. Rajendra, Jamry, Isabela, Berg, Douglas E., Berg, Claire M., Chaplin, David D.
We are applying a transposon-based approach for detecting and mapping features of special interest to construct ‘feature maps’ of currently uncharacterized portions of the human leukocyte antigen...
Wang, Gan, Whittam, Thomas S., Berg, Claire M., Berg, Douglas E.
The RAPD (random amplified polymorphic DNA) fingerprinting method, which utilizes low stringency PCR amplification with single primers of arbitrary sequence to generate strain-specific arrays of...
Shin, Dong-Guk, Lee, Changhwan, Zhang, Jinghui, Rudd, Kenneth E., Berg, Claire M.
This paper reports our exploratory work to redesign, implement and integrate a collection of genome software tools with an object-oriented database system. Our software tools deal with genome data...
Krishnan, Rajendra B., Kersulyte, Dangeruta, Brikun, Igor, Berg, Claire M., Berg, Douglas E.
The 264 bp mini-transposon Tn5supF was constructed to sequence DNAs cloned in phage λ without extensive shotgun subcloning or primer walking. Unique sequences near each transposon end serve as...
Liu, Lin, Whalen, William, Das, Asis, Berg, Claire M.
A new approach to determining the sequence of cloned DNA is described. Unique regions near each end of the transposable element gamma-delta provide a pair of “portable” primer-specific sites for...
Procedure for Isolating Mutants Defective in Metabolite Transport or Utilization
Berg, Claire M., Rossi, John J., Coleman, Joyce V., Conlon, Michele
Mutants defective in uptake or utilization of a given metabolite can readily be obtained from facultative auxotrophs (for that metabolite) by penicillin enrichment under nonpermissive conditions in...
Thymineless Death in polA+ and polA− Strains of Escherichia coli
Berg, Claire M., O'Neill, John M.
The extreme sensitivity of polA− cells to thymineless death is due, primarily, to the absence of an extended lag prior to the commencement of death. Once thymineless death has commenced, the rate...
Proline Excretion and Indirect Suppression in Escherichia coli and Salmonella typhimurium
Berg, Claire M., Rossi, John J.
The last step in proline biosynthesis in Escherichia coli K-12, Salmonella typhimurium LT7, and a number of other enterobacterial isolates is regulated so that no proline is excreted, even if excess...
Auxotroph Accumulation in Deoxyribonucleic Acid Polymeraseless Strains of Escherichia coli K-121
Spontaneous auxotrophs are found with high frequency in several strains of Escherichia coli K-12 deficient in Kornberg deoxyribonucleic acid polymerase. These include amino acid-, vitamin-, purine-,...
Differential Recovery of Auxotrophs After Penicillin Enrichment in Escherichia coli
Rossi, John J., Berg, Claire M.
Various auxotrophs are recovered from a penicillin enrichment cycle with differing efficiencies. Reconstruction experiments indicate that, under starvation conditions in the presence of penicillin,...
Kinetic and Genetic Analyses of d-Cycloserine Inhibition and Resistance in Escherichia coli
Curtiss, Roy, Charamella, Leigh J., Berg, Claire M., Harris, Paula E.
Curtiss, Roy, III (Oak Ridge National Laboratory, Oak Ridge, Tenn.), Leigh J. Charamella, Claire M. Berg, and Paula E. Harris. Kinetic and genetic analyses of d-cycloserine inhibition and resistance...
Inhibition of Amino Acid Transport in Escherichia coli by Some Beta-Lactam Antibiotics
Anderson, Scott V., Berg, Claire M.
Among 10 antibiotics tested, cephaloridine and cephalothin showed the greatest inhibition of proline active transport. Ethylenediaminetetraacetate pretreatment of the cells did not enhance inhibition...
Effect of Enrichment Procedure upon Auxotroph Recovery in Escherichia coli K-12
Rossi, John J., Berg, Claire M.
Proline-requiring auxotrophs are recovered preferentially after mutant enrichment procedures (e.g., penicillin) which perturb the cell envelope, but not after procedures (e.g., thymineless death)...
Penicillin-Induced Lysis of Escherichia coli in Medium That Does Not Support Sustained Growth
Berg, Claire M., Anderson, Scott V.
Exponentially growing cells of an Escherichia coli auxotroph that are washed and resuspended in buffer containing a carbon source undergo sufficient growth to render them susceptible to...
Berg, Claire M., Grullón, Carmen A., Wang, Aoquan, Whalen, William A., Berg, Douglas E.
Generalized transduction is commonly used to move transposon-induced mutations among bacterial strains by selecting for inheritance of a transposonencoded resistance determinant. Although complete...
High Frequency Generalized Transduction by Minimu Plasmid Phage
Wang, Boamin, Liu, Lin, Groisman, Eduardo A., Casadaban, Malcolm J., Berg, Claire M.
Deletion derivatives of phage Mu which replicate as multicopy plasmids, and also transpose and package like Mu, have been developed for the in vivo cloning of bacterial genes. We show here that these...
ESCHERICHIA COLI K-12 Auxotrophs Induced by Insertion of the Transposable Element Tn5
Shaw, Karen J., Berg, Claire M.
The sites of insertion of the transposable kanamycin-neomycin resistance-determining element, Tn5, in the E. coli K-12 chromosome were assessed in a collection of over 300 auxotrophs. Although...
Berg, Claire M., Shaw, Karen J., Vender, Joyce, Borucka-Mankiewicz, Maryla
The properties of 22 isoleucine-valine auxotrophs induced in Escherichia coli K-12 by the transposable element, Tn5, were characterized on the basis of growth requirements, cross-feeding behavior,...
Procedure for Isolating Mutants Defective in Metabolite Transport or Utilization
Berg, Claire M., Rossi, John J., Coleman, Joyce V., Conlon, Michele
Mutants defective in uptake or utilization of a given metabolite can readily be obtained from facultative auxotrophs (for that metabolite) by penicillin enrichment under nonpermissive conditions in...
Thymineless Death in polA+ and polA− Strains of Escherichia coli
Berg, Claire M., O'Neill, John M.
The extreme sensitivity of polA− cells to thymineless death is due, primarily, to the absence of an extended lag prior to the commencement of death. Once thymineless death has commenced, the rate...
Proline Excretion and Indirect Suppression in Escherichia coli and Salmonella typhimurium
Berg, Claire M., Rossi, John J.
The last step in proline biosynthesis in Escherichia coli K-12, Salmonella typhimurium LT7, and a number of other enterobacterial isolates is regulated so that no proline is excreted, even if excess...
Auxotroph Accumulation in Deoxyribonucleic Acid Polymeraseless Strains of Escherichia coli K-121
Spontaneous auxotrophs are found with high frequency in several strains of Escherichia coli K-12 deficient in Kornberg deoxyribonucleic acid polymerase. These include amino acid-, vitamin-, purine-,...
Differential Recovery of Auxotrophs After Penicillin Enrichment in Escherichia coli
Rossi, John J., Berg, Claire M.
Various auxotrophs are recovered from a penicillin enrichment cycle with differing efficiencies. Reconstruction experiments indicate that, under starvation conditions in the presence of penicillin,...
Kinetic and Genetic Analyses of d-Cycloserine Inhibition and Resistance in Escherichia coli
Curtiss, Roy, Charamella, Leigh J., Berg, Claire M., Harris, Paula E.
Curtiss, Roy, III (Oak Ridge National Laboratory, Oak Ridge, Tenn.), Leigh J. Charamella, Claire M. Berg, and Paula E. Harris. Kinetic and genetic analyses of d-cycloserine inhibition and resistance...
Inhibition of Amino Acid Transport in Escherichia coli by Some Beta-Lactam Antibiotics
Anderson, Scott V., Berg, Claire M.
Among 10 antibiotics tested, cephaloridine and cephalothin showed the greatest inhibition of proline active transport. Ethylenediaminetetraacetate pretreatment of the cells did not enhance inhibition...
Effect of Enrichment Procedure upon Auxotroph Recovery in Escherichia coli K-12
Rossi, John J., Berg, Claire M.
Proline-requiring auxotrophs are recovered preferentially after mutant enrichment procedures (e.g., penicillin) which perturb the cell envelope, but not after procedures (e.g., thymineless death)...
Penicillin-Induced Lysis of Escherichia coli in Medium That Does Not Support Sustained Growth
Berg, Claire M., Anderson, Scott V.
Exponentially growing cells of an Escherichia coli auxotroph that are washed and resuspended in buffer containing a carbon source undergo sufficient growth to render them susceptible to...
Berg, Claire M., Grullón, Carmen A., Wang, Aoquan, Whalen, William A., Berg, Douglas E.
Generalized transduction is commonly used to move transposon-induced mutations among bacterial strains by selecting for inheritance of a transposonencoded resistance determinant. Although complete...
High Frequency Generalized Transduction by Minimu Plasmid Phage
Wang, Boamin, Liu, Lin, Groisman, Eduardo A., Casadaban, Malcolm J., Berg, Claire M.
Deletion derivatives of phage Mu which replicate as multicopy plasmids, and also transpose and package like Mu, have been developed for the in vivo cloning of bacterial genes. We show here that these...
ESCHERICHIA COLI K-12 Auxotrophs Induced by Insertion of the Transposable Element Tn5
Shaw, Karen J., Berg, Claire M.
The sites of insertion of the transposable kanamycin-neomycin resistance-determining element, Tn5, in the E. coli K-12 chromosome were assessed in a collection of over 300 auxotrophs. Although...
Berg, Claire M., Shaw, Karen J., Vender, Joyce, Borucka-Mankiewicz, Maryla
The properties of 22 isoleucine-valine auxotrophs induced in Escherichia coli K-12 by the transposable element, Tn5, were characterized on the basis of growth requirements, cross-feeding behavior,...