Claire M. Fraser

Whole-Genome Analysis of Human Influenza A Virus Reveals Multiple Persistent Lineages and Reassortment among Recent H3N2 Viruses (2005)

Edward C. Holmes, Elodie Ghedin, Naomi Miller, Jill Taylor, Yiming Bao, Kirsten St. George, ...

Evolution of the flu virus is analyzed via genomic phylogeny; humans are found to provide a reservoir of antigenic variability implicit in flu adaptation and virulence.

Whole-Genome Analysis of Human Influenza A Virus Reveals Multiple Persistent Lineages and Reassortment among Recent H3N2 Viruses (2005)

Edward C. Holmes, Elodie Ghedin, Naomi Miller, Jill Taylor, Yiming Bao, Kirsten St. George, ...

Understanding the evolution of influenza A viruses in humans is important for surveillance and vaccine strain selection. We performed a phylogenetic analysis of 156 complete genomes of human H3N2...

Major Structural Differences and Novel Potential Virulence Mechanisms from the Genomes of Multiple Campylobacter Species (2005)

Derrick E. Fouts, Emmanuel F. Mongodin, Robert E. Mandrell, William G. Miller, David A. Rasko, Jacques Ravel, ...

Although the single genome sequence of the human Campylobacter jejuni yielded some insight, comparison of multiple species of the same genus elucidated greater understanding of virulence mechanisms.

Major Structural Differences and Novel Potential Virulence Mechanisms from the Genomes of Multiple Campylobacter Species (2005)

Derrick E. Fouts, Emmanuel F. Mongodin, Robert E. Mandrell, William G. Miller, David A. Rasko, Jacques Ravel, ...

Sequencing and comparative genome analysis of four strains of Campylobacter including C. lari RM2100, C. upsaliensis RM3195, and C. coli RM2228 has revealed major structural differences that are...

Whole-Genome Analysis of Human Influenza A Virus Reveals Multiple Persistent Lineages and Reassortment among Recent H3N2 Viruses (2005)

Holmes, Edward C., Ghedin, Elodie, Miller, Naomi, Taylor, Jill, Bao, Yiming, St. George, Kirsten, ...

Understanding the evolution of influenza A viruses in humans is important for surveillance and vaccine strain selection. We performed a phylogenetic analysis of 156 complete genomes of human H3N2...

Whole-Genome Analysis of Human Influenza A Virus Reveals Multiple Persistent Lineages and Reassortment among Recent H3N2 Viruses (2005)

Holmes, Edward C., Ghedin, Elodie, Miller, Naomi, Taylor, Jill, Bao, Yiming, St. George, Kirsten, ...

Understanding the evolution of influenza A viruses in humans is important for surveillance and vaccine strain selection. We performed a phylogenetic analysis of 156 complete genomes of human H3N2...

Whole-genome analysis of human influenza A virus reveals multiple persistent lineages and reassortment among recent H3N2 viruses. PLoS Biol (2005)

Edward C. Holmes, Elodie Ghedin, Naomi Miller, Jill Taylor, Yiming Bao, Kirsten St. George, ...

Understanding the evolution of influenza A viruses in humans is important for surveillance and vaccine strain selection. We performed a phylogenetic analysis of 156 complete genomes of human H3N2...

Genomic Insights into Methanotrophy: The Complete Genome Sequence of Methylococcus capsulatus (Bath) (2004)

Naomi Ward, Øivind Larsen, James Sakwa, Live Bruseth, Hoda Khouri, A. Scott Durkin, ...

Methanotrophs are bacteria that use methane as a sole carbon source. The genome sequence of Methylococcus capsulatus deepens our understanding of methanotroph biology and its relationship to global...

Genomic Insights into Methanotrophy: The Complete Genome Sequence of Methylococcus capsulatus (Bath) (2004)

Naomi Ward, Øivind Larsen, James Sakwa, Live Bruseth, Hoda Khouri, A. Scott Durkin, ...

Methanotrophs are ubiquitous bacteria that can use the greenhouse gas methane as a sole carbon and energy source for growth, thus playing major roles in global carbon cycles, and in particular,...

Genomic Insights into Methanotrophy: The Complete Genome Sequence of Methylococcus capsulatus (Bath) (2004)

Ward, Naomi, Larsen, Øivind, Sakwa, James, Bruseth, Live, Khouri, Hoda, Durkin, A. Scott, ...

Methanotrophs are ubiquitous bacteria that can use the greenhouse gas methane as a sole carbon and energy source for growth, thus playing major roles in global carbon cycles, and in particular,...

Genomic Insights into Methanotrophy : the Complete Genome Sequence of Methylococcus capsulatus (Bath) (2004)

Ward, Naomi, Larsen, Øivind, Sakwa, James, Bruseth, Live, Khouri, Hoda, Durkin, A. Scott, ...

Methanotrophs are ubiquitous bacteria that can use the greenhouse gas methane as a sole carbon and energy source for growth, thus playing major roles in global carbon cycles, and in particular,...

An integrated 4249 marker FISH/RH map of the canine genome (2004)

Breen, Matthew, Hitte, Christophe, Lorentzen, Travis D, Thomas, Rachael, Cadieu, Edouard, Sabacan, Leah, ...

Abstract Background The 156 breeds of dog recognized by the American Kennel Club offer a unique opportunity to map genes important in genetic variation. Each breed features a defining constellation...

Human, Mouse, and Rat Genome Large-Scale Rearrangements: Stability Versus Speciation (2004)

Zhao, Shaying, Shetty, Jyoti, Hou, Lihua, Delcher, Arthur, Zhu, Baoli, Osoegawa, Kazutoyo, ...

Using paired-end sequences from bacterial artificial chromosomes, we have constructed high-resolution synteny and rearrangement breakpoint maps among human, mouse, and rat genomes. Among the >300...

Whole genome comparisons of serotype 4b and 1/2a strains of the food-borne pathogen Listeria monocytogenes reveal new insights into the core genome components of this species (2004)

Nelson, Karen E., Fouts, Derrick E., Mongodin, Emmanuel F., Ravel, Jacques, DeBoy, Robert T., Kolonay, James F., ...

The genomes of three strains of Listeria monocytogenes that have been associated with food‐borne illness in the USA were subjected to whole genome comparative analysis. A total of 51, 97 and 69...

Human, Mouse, and Rat Genome Large-Scale Rearrangements: Stability Versus Speciation (2004)

Zhao, Shaying, Shetty, Jyoti, Hou, Lihua, Delcher, Arthur, Zhu, Baoli, Osoegawa, Kazutoyo, ...

Using paired-end sequences from bacterial artificial chromosomes, we have constructed high-resolution synteny and rearrangement breakpoint maps among human, mouse, and rat genomes. Among the >300...

The genome sequence of Bacillus cereus ATCC 10987 reveals metabolic adaptations and a large plasmid related to Bacillus anthracis pXO1 (2004)

Rasko, David A., Ravel, Jacques, Økstad, Ole Andreas, Helgason, Erlendur, Cer, Regina Z., Jiang, Lingxia, ...

We sequenced the complete genome of Bacillus cereus ATCC 10987, a non‐lethal dairy isolate in the same genetic subgroup as Bacillus anthracis. Comparison of the chromosomes demonstrated that...

The sequence and analysis of Trypanosoma brucei chromosome II (2003)

Ghedin, Elodie, Song, Jinming, MacLeod, Annette, Bringaud, Frederic, Larkin, Christopher, ...

We report here the sequence of chromosome II from Trypanosoma brucei, the causative agent of African sleeping sickness. The 1.2‐Mb pairs encode about 470 predicted genes organised in 17...

The complexity of simplicity (2001)

Peterson, Scott N, Fraser, Claire M

Abstract What is the minimum number of genes or functions necessary to support cellular life? The concept of a 'minimal genome' has become popular, but is it a useful concept, and if so, what might a...

Complete genome sequence of Caulobacter crescentus

Nierman, William C., Feldblyum, Tamara V., Laub, Michael T., Paulsen, Ian T., Nelson, Karen E., Eisen, Jonathan, ...

The complete genome sequence of Caulobacter crescentus was determined to be 4,016,942 base pairs in a single circular chromosome encoding 3,767 genes. This organism, which grows in a dilute aquatic...

Characterization of Porphyromonas gingivalis Insertion Sequence-Like Element ISPg5

Califano, Joseph V., Kitten, Todd, Lewis, Janina P., Macrina, Francis L., Fleischmann, Robert D., Fraser, Claire M., ...

Porphyromonas gingivalis, a black-pigmented, gram-negative anaerobe, is found in periodontitis lesions, and its presence in subgingival plaque significantly increases the risk for periodontitis. In...

Genomic Loci of the Porphyromonas gingivalis Insertion Element IS1126

Dong, Hong, Chen, Tsute, Dewhirst, Floyd E., Fleischmann, Robert D., Fraser, Claire M., Duncan, Margaret J.

The Porphyromonas gingivalis genome contains multiple copies of insertion element IS1126. When chromosomal DNA digests of different strains were probed with IS1126, between 25 and 35 hybridizing...

The complete genome sequence of Chlorobium tepidum TLS, a photosynthetic, anaerobic, green-sulfur bacterium

Eisen, Jonathan A., Nelson, Karen E., Paulsen, Ian T., Heidelberg, John F., Wu, Martin, Dodson, Robert J., ...

The complete genome of the green-sulfur eubacterium Chlorobium tepidum TLS was determined to be a single circular chromosome of 2,154,946 bp. This represents the first genome sequence from the phylum...

Identification of in vivo expressed vaccine candidate antigens from Staphylococcus aureus

Etz, Hildegard, Minh, Duc Bui, Henics, Tamás, Dryla, Agnieszka, Winkler, Birgit, Triska, Christine, ...

For the design of potent subunit vaccines, it is of paramount importance to identify all antigens immunologically recognized by a patient population infected with a pathogen. We have developed a...

Complete genome sequence and comparative genomic analysis of an emerging human pathogen, serotype V Streptococcus agalactiae

Tettelin, Hervé, Masignani, Vega, Cieslewicz, Michael J., Eisen, Jonathan A., Peterson, Scott, Wessels, Michael R., ...

The 2,160,267 bp genome sequence of Streptococcus agalactiae, the leading cause of bacterial sepsis, pneumonia, and meningitis in neonates in the U.S. and Europe, is predicted to encode 2,175 genes....

The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts

Paulsen, Ian T., Seshadri, Rekha, Nelson, Karen E., Eisen, Jonathan A., Heidelberg, John F., Read, Timothy D., ...

The 3.31-Mb genome sequence of the intracellular pathogen and potential bioterrorism agent, Brucella suis, was determined. Comparison of B. suis with Brucella melitensis has defined a finite set of...

The complexity of simplicity

Peterson, Scott N, Fraser, Claire M

What is the minimum number of genes or functions necessary to support cellular life? The concept of a 'minimal genome' has become popular, but is it a useful concept, and if so, what might a minimal...

Complete genome sequence of the Q-fever pathogen Coxiella burnetii

Seshadri, Rekha, Paulsen, Ian T., Eisen, Jonathan A., Read, Timothy D., Nelson, Karen E., Nelson, William C., ...

The 1,995,275-bp genome of Coxiella burnetii, Nine Mile phase I RSA493, a highly virulent zoonotic pathogen and category B bioterrorism agent, was sequenced by the random shotgun method. This...

Functional Selection of Vaccine Candidate Peptides from Staphylococcus aureus Whole-Genome Expression Libraries In Vitro

Weichhart, Thomas, Horky, Markus, Söllner, Johannes, Gangl, Susanne, Henics, Tamàs, Nagy, Eszter, ...

An in vitro protein selection method, ribosome display, has been applied to comprehensively identify and map the immunologically relevant proteins of the human pathogen Staphylococcus aureus. A...

The sequence and analysis of Trypanosoma brucei chromosome II

Ghedin, Elodie, Song, Jinming, MacLeod, Annette, Bringaud, Frederic, Larkin, Christopher, ...

We report here the sequence of chromosome II from Trypanosoma brucei, the causative agent of African sleeping sickness. The 1.2-Mb pairs encode about 470 predicted genes organised in 17 directional...

Complete Genome Sequence of the Broad-Host-Range Vibriophage KVP40: Comparative Genomics of a T4-Related Bacteriophage

Miller, Eric S., Heidelberg, John F., Eisen, Jonathan A., Nelson, William C., Durkin, A. Scott, Ciecko, Ann, ...

The complete genome sequence of the T4-like, broad-host-range vibriophage KVP40 has been determined. The genome sequence is 244,835 bp, with an overall G+C content of 42.6%. It encodes 386 putative...

The complete genome sequence of the Arabidopsis and tomato pathogen Pseudomonas syringae pv. tomato DC3000

Buell, C. Robin, Joardar, Vinita, Lindeberg, Magdalen, Selengut, Jeremy, Paulsen, Ian T., Gwinn, Michelle L., ...

We report the complete genome sequence of the model bacterial pathogen Pseudomonas syringae pathovar tomato DC3000 (DC3000), which is pathogenic on tomato and Arabidopsis thaliana. The DC3000 genome...

Complete Genome Sequence of the Oral Pathogenic Bacterium Porphyromonas gingivalis Strain W83

Nelson, Karen E., Fleischmann, Robert D., DeBoy, Robert T., Paulsen, Ian T., Fouts, Derrick E., Eisen, Jonathan A., ...

The complete 2,343,479-bp genome sequence of the gram-negative, pathogenic oral bacterium Porphyromonas gingivalis strain W83, a major contributor to periodontal disease, was determined. Whole-genome...

Mouse BAC Ends Quality Assessment and Sequence Analyses

Zhao, Shaying, Shatsman, Sofiya, Ayodeji, Bola, Geer, Keita, Tsegaye, Getahun, Krol, Margaret, ...

A large-scale BAC end-sequencing project at The Institute for Genomic Research (TIGR) has generated one of the most extensive sets of sequence markers for the mouse genome to date. With a sequencing...

The genome sequence of Bacillus cereus ATCC 10987 reveals metabolic adaptations and a large plasmid related to Bacillus anthracis pXO1

Rasko, David A., Ravel, Jacques, Økstad, Ole Andreas, Helgason, Erlendur, Cer, Regina Z., Jiang, Lingxia, ...

We sequenced the complete genome of Bacillus cereus ATCC 10987, a non-lethal dairy isolate in the same genetic subgroup as Bacillus anthracis. Comparison of the chromosomes demonstrated that B.cereus...

Comparison of the genome of the oral pathogen Treponema denticola with other spirochete genomes

Seshadri, Rekha, Myers, Garry S. A., Tettelin, Hervé, Eisen, Jonathan A., Heidelberg, John F., Dodson, Robert J., ...

We present the complete 2,843,201-bp genome sequence of Treponema denticola (ATCC 35405) an oral spirochete associated with periodontal disease. Analysis of the T. denticola genome reveals factors...

Whole genome comparisons of serotype 4b and 1/2a strains of the food-borne pathogen Listeria monocytogenes reveal new insights into the core genome components of this species

Nelson, Karen E., Fouts, Derrick E., Mongodin, Emmanuel F., Ravel, Jacques, DeBoy, Robert T., Kolonay, James F., ...

The genomes of three strains of Listeria monocytogenes that have been associated with food-borne illness in the USA were subjected to whole genome comparative analysis. A total of 51, 97 and 69...

Identification of anthrax toxin genes in a Bacillus cereus associated with an illness resembling inhalation anthrax

Hoffmaster, Alex R., Ravel, Jacques, Rasko, David A., Chapman, Gail D., Chute, Michael D., Marston, Chung K., ...

Bacillus anthracis is the etiologic agent of anthrax, an acute fatal disease among mammals. It was thought to differ from Bacillus cereus, an opportunistic pathogen and cause of food poisoning, by...

Genomic Insights into Methanotrophy: The Complete Genome Sequence of Methylococcus capsulatus (Bath)

Ward, Naomi, Larsen, Øivind, Sakwa, James, Bruseth, Live, Khouri, Hoda, Durkin, A. Scott, ...

Methanotrophs are ubiquitous bacteria that can use the greenhouse gas methane as a sole carbon and energy source for growth, thus playing major roles in global carbon cycles, and in particular,...

Phylogenetic discovery bias in Bacillus anthracis using single-nucleotide polymorphisms from whole-genome sequencing

Pearson, Talima, Busch, Joseph D., Ravel, Jacques, Read, Timothy D., Rhoton, Shane D., U'Ren, Jana M., ...

Phylogenetic reconstruction using molecular data is often subject to homoplasy, leading to inaccurate conclusions about phylogenetic relationships among operational taxonomic units. Compared with...

Genetic exchange and plasmid transfers in Borrelia burgdorferi sensu stricto revealed by three-way genome comparisons and multilocus sequence typing

Qiu, Wei-Gang, Schutzer, Steven E., Bruno, John F., Attie, Oliver, Xu, Yun, Dunn, John J., ...

Comparative genomics of closely related bacterial isolates is a powerful method for uncovering virulence and other important genome elements. We determined draft sequences (8-fold coverage) of the...

Structural flexibility in the Burkholderia mallei genome

Nierman, William C., DeShazer, David, Kim, H. Stanley, Tettelin, Herve, Nelson, Karen E., Feldblyum, Tamara, ...

The complete genome sequence of Burkholderia mallei ATCC 23344 provides insight into this highly infectious bacterium's pathogenicity and evolutionary history. B. mallei, the etiologic agent of...

Human, Mouse, and Rat Genome Large-Scale Rearrangements: Stability Versus Speciation

Zhao, Shaying, Shetty, Jyoti, Hou, Lihua, Delcher, Arthur, Zhu, Baoli, Osoegawa, Kazutoyo, ...

Using paired-end sequences from bacterial artificial chromosomes, we have constructed high-resolution synteny and rearrangement breakpoint maps among human, mouse, and rat genomes. Among the >300...

Major Structural Differences and Novel Potential Virulence Mechanisms from the Genomes of Multiple Campylobacter Species

Fouts, Derrick E, Mongodin, Emmanuel F, Mandrell, Robert E, Miller, William G, Rasko, David A, Ravel, Jacques, ...

Sequencing and comparative genome analysis of four strains of Campylobacter including C. lari RM2100, C. upsaliensis RM3195, and C. coli RM2228 has revealed major structural differences that are...

Gene Transfer and Genome Plasticity in Thermotoga maritima, a Model Hyperthermophilic Species†

Mongodin, Emmanuel F., Hance, Ioana R., DeBoy, Robert T., Gill, Steven R., Daugherty, Sean, Huber, Robert, ...

The genome sequence of the hyperthermophilic bacterium Thermotoga maritima MSB8 presents evidence for lateral gene transfer events between bacterial and archaeal species. To estimate the extent of...

The psychrophilic lifestyle as revealed by the genome sequence of Colwellia psychrerythraea 34H through genomic and proteomic analyses

Methé, Barbara A., Nelson, Karen E., Deming, Jody W., Momen, Bahram, Melamud, Eugene, Zhang, Xijun, ...

The completion of the 5,373,180-bp genome sequence of the marine psychrophilic bacterium Colwellia psychrerythraea 34H, a model for the study of life in permanently cold environments, reveals...

Whole-Genome Analysis of Human Influenza A Virus Reveals Multiple Persistent Lineages and Reassortment among Recent H3N2 Viruses

Holmes, Edward C, Ghedin, Elodie, Miller, Naomi, Taylor, Jill, Bao, Yiming, St George, Kirsten, ...

Understanding the evolution of influenza A viruses in humans is important for surveillance and vaccine strain selection. We performed a phylogenetic analysis of 156 complete genomes of human H3N2...

Genome analysis of multiple pathogenic isolates of Streptococcus agalactiae: Implications for the microbial “pan-genome”

Tettelin, Hervé, Masignani, Vega, Cieslewicz, Michael J., Donati, Claudio, Medini, Duccio, Ward, Naomi L., ...

The development of efficient and inexpensive genome sequencing methods has revolutionized the study of human bacterial pathogens and improved vaccine design. Unfortunately, the sequence of a single...

Whole-Genome Sequence Analysis of Pseudomonas syringae pv. phaseolicola 1448A Reveals Divergence among Pathovars in Genes Involved in Virulence and Transposition†

Joardar, Vinita, Lindeberg, Magdalen, Jackson, Robert W., Selengut, Jeremy, Dodson, Robert, Brinkac, Lauren M., ...

Pseudomonas syringae pv. phaseolicola, a gram-negative bacterial plant pathogen, is the causal agent of halo blight of bean. In this study, we report on the genome sequence of P. syringae pv....

Modeling Bacterial Evolution with Comparative-Genome-Based Marker Systems: Application to Mycobacterium tuberculosis Evolution and Pathogenesis

Alland, David, Whittam, Thomas S., Murray, Megan B., Cave, M. Donald, Hazbon, Manzour H., Dix, Kim, ...

The comparative-genomic sequencing of two Mycobacterium tuberculosis strains enabled us to identify single nucleotide polymorphism (SNP) markers for studies of evolution, pathogenesis, and...

Theileria parva candidate vaccine antigens recognized by immune bovine cytotoxic T lymphocytes

Graham, Simon P., Pellé, Roger, Honda, Yoshikazu, Mwangi, Duncan M., Tonukari, Nyerhovwo J., Yamage, Mat, ...

East Coast fever, caused by the tick-borne intracellular apicomplexan parasite Theileria parva, is a highly fatal lymphoproliferative disease of cattle. The pathogenic schizont-induced lymphocyte...

Complete genome sequence of Caulobacter crescentus

Nierman, William C., Feldblyum, Tamara V., Laub, Michael T., Paulsen, Ian T., Nelson, Karen E., Eisen, Jonathan, ...

The complete genome sequence of Caulobacter crescentus was determined to be 4,016,942 base pairs in a single circular chromosome encoding 3,767 genes. This organism, which grows in a dilute aquatic...

Characterization of Porphyromonas gingivalis Insertion Sequence-Like Element ISPg5

Califano, Joseph V., Kitten, Todd, Lewis, Janina P., Macrina, Francis L., Fleischmann, Robert D., Fraser, Claire M., ...

Porphyromonas gingivalis, a black-pigmented, gram-negative anaerobe, is found in periodontitis lesions, and its presence in subgingival plaque significantly increases the risk for periodontitis. In...

Genomic Loci of the Porphyromonas gingivalis Insertion Element IS1126

Dong, Hong, Chen, Tsute, Dewhirst, Floyd E., Fleischmann, Robert D., Fraser, Claire M., Duncan, Margaret J.

The Porphyromonas gingivalis genome contains multiple copies of insertion element IS1126. When chromosomal DNA digests of different strains were probed with IS1126, between 25 and 35 hybridizing...

The complete genome sequence of Chlorobium tepidum TLS, a photosynthetic, anaerobic, green-sulfur bacterium

Eisen, Jonathan A., Nelson, Karen E., Paulsen, Ian T., Heidelberg, John F., Wu, Martin, Dodson, Robert J., ...

The complete genome of the green-sulfur eubacterium Chlorobium tepidum TLS was determined to be a single circular chromosome of 2,154,946 bp. This represents the first genome sequence from the phylum...

Identification of in vivo expressed vaccine candidate antigens from Staphylococcus aureus

Etz, Hildegard, Minh, Duc Bui, Henics, Tamás, Dryla, Agnieszka, Winkler, Birgit, Triska, Christine, ...

For the design of potent subunit vaccines, it is of paramount importance to identify all antigens immunologically recognized by a patient population infected with a pathogen. We have developed a...

Complete genome sequence and comparative genomic analysis of an emerging human pathogen, serotype V Streptococcus agalactiae

Tettelin, Hervé, Masignani, Vega, Cieslewicz, Michael J., Eisen, Jonathan A., Peterson, Scott, Wessels, Michael R., ...

The 2,160,267 bp genome sequence of Streptococcus agalactiae, the leading cause of bacterial sepsis, pneumonia, and meningitis in neonates in the U.S. and Europe, is predicted to encode 2,175 genes....

The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts

Paulsen, Ian T., Seshadri, Rekha, Nelson, Karen E., Eisen, Jonathan A., Heidelberg, John F., Read, Timothy D., ...

The 3.31-Mb genome sequence of the intracellular pathogen and potential bioterrorism agent, Brucella suis, was determined. Comparison of B. suis with Brucella melitensis has defined a finite set of...

The complexity of simplicity

Peterson, Scott N, Fraser, Claire M

What is the minimum number of genes or functions necessary to support cellular life? The concept of a 'minimal genome' has become popular, but is it a useful concept, and if so, what might a minimal...

Complete genome sequence of the Q-fever pathogen Coxiella burnetii

Seshadri, Rekha, Paulsen, Ian T., Eisen, Jonathan A., Read, Timothy D., Nelson, Karen E., Nelson, William C., ...

The 1,995,275-bp genome of Coxiella burnetii, Nine Mile phase I RSA493, a highly virulent zoonotic pathogen and category B bioterrorism agent, was sequenced by the random shotgun method. This...

Modeling Bacterial Evolution with Comparative-Genome-Based Marker Systems: Application to Mycobacterium tuberculosis Evolution and Pathogenesis

Alland, David, Whittam, Thomas S., Murray, Megan B., Cave, M. Donald, Hazbon, Manzour H., Dix, Kim, ...

The comparative-genomic sequencing of two Mycobacterium tuberculosis strains enabled us to identify single nucleotide polymorphism (SNP) markers for studies of evolution, pathogenesis, and...

Functional Selection of Vaccine Candidate Peptides from Staphylococcus aureus Whole-Genome Expression Libraries In Vitro

Weichhart, Thomas, Horky, Markus, Söllner, Johannes, Gangl, Susanne, Henics, Tamàs, Nagy, Eszter, ...

An in vitro protein selection method, ribosome display, has been applied to comprehensively identify and map the immunologically relevant proteins of the human pathogen Staphylococcus aureus. A...

The sequence and analysis of Trypanosoma brucei chromosome II

Ghedin, Elodie, Song, Jinming, MacLeod, Annette, Bringaud, Frederic, Larkin, Christopher, ...

We report here the sequence of chromosome II from Trypanosoma brucei, the causative agent of African sleeping sickness. The 1.2-Mb pairs encode about 470 predicted genes organised in 17 directional...

Complete Genome Sequence of the Broad-Host-Range Vibriophage KVP40: Comparative Genomics of a T4-Related Bacteriophage

Miller, Eric S., Heidelberg, John F., Eisen, Jonathan A., Nelson, William C., Durkin, A. Scott, Ciecko, Ann, ...

The complete genome sequence of the T4-like, broad-host-range vibriophage KVP40 has been determined. The genome sequence is 244,835 bp, with an overall G+C content of 42.6%. It encodes 386 putative...

The complete genome sequence of the Arabidopsis and tomato pathogen Pseudomonas syringae pv. tomato DC3000

Buell, C. Robin, Joardar, Vinita, Lindeberg, Magdalen, Selengut, Jeremy, Paulsen, Ian T., Gwinn, Michelle L., ...

We report the complete genome sequence of the model bacterial pathogen Pseudomonas syringae pathovar tomato DC3000 (DC3000), which is pathogenic on tomato and Arabidopsis thaliana. The DC3000 genome...

Complete Genome Sequence of the Oral Pathogenic Bacterium Porphyromonas gingivalis Strain W83

Nelson, Karen E., Fleischmann, Robert D., DeBoy, Robert T., Paulsen, Ian T., Fouts, Derrick E., Eisen, Jonathan A., ...

The complete 2,343,479-bp genome sequence of the gram-negative, pathogenic oral bacterium Porphyromonas gingivalis strain W83, a major contributor to periodontal disease, was determined. Whole-genome...

Mouse BAC Ends Quality Assessment and Sequence Analyses

Zhao, Shaying, Shatsman, Sofiya, Ayodeji, Bola, Geer, Keita, Tsegaye, Getahun, Krol, Margaret, ...

A large-scale BAC end-sequencing project at The Institute for Genomic Research (TIGR) has generated one of the most extensive sets of sequence markers for the mouse genome to date. With a sequencing...

The genome sequence of Bacillus cereus ATCC 10987 reveals metabolic adaptations and a large plasmid related to Bacillus anthracis pXO1

Rasko, David A., Ravel, Jacques, Økstad, Ole Andreas, Helgason, Erlendur, Cer, Regina Z., Jiang, Lingxia, ...

We sequenced the complete genome of Bacillus cereus ATCC 10987, a non-lethal dairy isolate in the same genetic subgroup as Bacillus anthracis. Comparison of the chromosomes demonstrated that B.cereus...

Comparison of the genome of the oral pathogen Treponema denticola with other spirochete genomes

Seshadri, Rekha, Myers, Garry S. A., Tettelin, Hervé, Eisen, Jonathan A., Heidelberg, John F., Dodson, Robert J., ...

We present the complete 2,843,201-bp genome sequence of Treponema denticola (ATCC 35405) an oral spirochete associated with periodontal disease. Analysis of the T. denticola genome reveals factors...

Whole genome comparisons of serotype 4b and 1/2a strains of the food-borne pathogen Listeria monocytogenes reveal new insights into the core genome components of this species

Nelson, Karen E., Fouts, Derrick E., Mongodin, Emmanuel F., Ravel, Jacques, DeBoy, Robert T., Kolonay, James F., ...

The genomes of three strains of Listeria monocytogenes that have been associated with food-borne illness in the USA were subjected to whole genome comparative analysis. A total of 51, 97 and 69...

Identification of anthrax toxin genes in a Bacillus cereus associated with an illness resembling inhalation anthrax

Hoffmaster, Alex R., Ravel, Jacques, Rasko, David A., Chapman, Gail D., Chute, Michael D., Marston, Chung K., ...

Bacillus anthracis is the etiologic agent of anthrax, an acute fatal disease among mammals. It was thought to differ from Bacillus cereus, an opportunistic pathogen and cause of food poisoning, by...

Genomic Insights into Methanotrophy: The Complete Genome Sequence of Methylococcus capsulatus (Bath)

Ward, Naomi, Larsen, Øivind, Sakwa, James, Bruseth, Live, Khouri, Hoda, Durkin, A. Scott, ...

Methanotrophs are ubiquitous bacteria that can use the greenhouse gas methane as a sole carbon and energy source for growth, thus playing major roles in global carbon cycles, and in particular,...

Phylogenetic discovery bias in Bacillus anthracis using single-nucleotide polymorphisms from whole-genome sequencing

Pearson, Talima, Busch, Joseph D., Ravel, Jacques, Read, Timothy D., Rhoton, Shane D., U'Ren, Jana M., ...

Phylogenetic reconstruction using molecular data is often subject to homoplasy, leading to inaccurate conclusions about phylogenetic relationships among operational taxonomic units. Compared with...

Genetic exchange and plasmid transfers in Borrelia burgdorferi sensu stricto revealed by three-way genome comparisons and multilocus sequence typing

Qiu, Wei-Gang, Schutzer, Steven E., Bruno, John F., Attie, Oliver, Xu, Yun, Dunn, John J., ...

Comparative genomics of closely related bacterial isolates is a powerful method for uncovering virulence and other important genome elements. We determined draft sequences (8-fold coverage) of the...

Structural flexibility in the Burkholderia mallei genome

Nierman, William C., DeShazer, David, Kim, H. Stanley, Tettelin, Herve, Nelson, Karen E., Feldblyum, Tamara, ...

The complete genome sequence of Burkholderia mallei ATCC 23344 provides insight into this highly infectious bacterium's pathogenicity and evolutionary history. B. mallei, the etiologic agent of...

Human, Mouse, and Rat Genome Large-Scale Rearrangements: Stability Versus Speciation

Zhao, Shaying, Shetty, Jyoti, Hou, Lihua, Delcher, Arthur, Zhu, Baoli, Osoegawa, Kazutoyo, ...

Using paired-end sequences from bacterial artificial chromosomes, we have constructed high-resolution synteny and rearrangement breakpoint maps among human, mouse, and rat genomes. Among the >300...

Major Structural Differences and Novel Potential Virulence Mechanisms from the Genomes of Multiple Campylobacter Species

Fouts, Derrick E, Mongodin, Emmanuel F, Mandrell, Robert E, Miller, William G, Rasko, David A, Ravel, Jacques, ...

Sequencing and comparative genome analysis of four strains of Campylobacter including C. lari RM2100, C. upsaliensis RM3195, and C. coli RM2228 has revealed major structural differences that are...

Gene Transfer and Genome Plasticity in Thermotoga maritima, a Model Hyperthermophilic Species†

Mongodin, Emmanuel F., Hance, Ioana R., DeBoy, Robert T., Gill, Steven R., Daugherty, Sean, Huber, Robert, ...

The genome sequence of the hyperthermophilic bacterium Thermotoga maritima MSB8 presents evidence for lateral gene transfer events between bacterial and archaeal species. To estimate the extent of...

The psychrophilic lifestyle as revealed by the genome sequence of Colwellia psychrerythraea 34H through genomic and proteomic analyses

Methé, Barbara A., Nelson, Karen E., Deming, Jody W., Momen, Bahram, Melamud, Eugene, Zhang, Xijun, ...

The completion of the 5,373,180-bp genome sequence of the marine psychrophilic bacterium Colwellia psychrerythraea 34H, a model for the study of life in permanently cold environments, reveals...

Whole-Genome Analysis of Human Influenza A Virus Reveals Multiple Persistent Lineages and Reassortment among Recent H3N2 Viruses

Holmes, Edward C, Ghedin, Elodie, Miller, Naomi, Taylor, Jill, Bao, Yiming, St George, Kirsten, ...

Understanding the evolution of influenza A viruses in humans is important for surveillance and vaccine strain selection. We performed a phylogenetic analysis of 156 complete genomes of human H3N2...

Genome analysis of multiple pathogenic isolates of Streptococcus agalactiae: Implications for the microbial “pan-genome”

Tettelin, Hervé, Masignani, Vega, Cieslewicz, Michael J., Donati, Claudio, Medini, Duccio, Ward, Naomi L., ...

The development of efficient and inexpensive genome sequencing methods has revolutionized the study of human bacterial pathogens and improved vaccine design. Unfortunately, the sequence of a single...

Whole-Genome Sequence Analysis of Pseudomonas syringae pv. phaseolicola 1448A Reveals Divergence among Pathovars in Genes Involved in Virulence and Transposition†

Joardar, Vinita, Lindeberg, Magdalen, Jackson, Robert W., Selengut, Jeremy, Dodson, Robert, Brinkac, Lauren M., ...

Pseudomonas syringae pv. phaseolicola, a gram-negative bacterial plant pathogen, is the causal agent of halo blight of bean. In this study, we report on the genome sequence of P. syringae pv....

Theileria parva candidate vaccine antigens recognized by immune bovine cytotoxic T lymphocytes

Graham, Simon P., Pellé, Roger, Honda, Yoshikazu, Mwangi, Duncan M., Tonukari, Nyerhovwo J., Yamage, Mat, ...

East Coast fever, caused by the tick-borne intracellular apicomplexan parasite Theileria parva, is a highly fatal lymphoproliferative disease of cattle. The pathogenic schizont-induced lymphocyte...

Strain-Specific Single-Nucleotide Polymorphism Assays for the Bacillus anthracis Ames Strain† ▿

Van Ert, Matthew N., Easterday, W. Ryan, Simonson, Tatum S., U'Ren, Jana M., Pearson, Talima, Kenefic, Leo J., ...

Highly precise diagnostics and forensic assays can be developed through a combination of evolutionary analysis and the exhaustive examination of genomic sequences. In Bacillus anthracis, whole-genome...