The importance of boundaries (2007)
In this paper the former Australian Electoral Commissioner considers the issue of electorate boundary changes. The paper provides a comprehensive review of the 2006 redistributions in NSW and...
The importance of boundaries (2007)
In this paper the former Australian Electoral Commissioner considers the issue of electorate boundary changes. The paper provides a comprehensive review of the 2006 redistributions in NSW and...
A study of training and development (T&D) in the Irish financial services sector (2006)
The proliferation of reports on 'lifelong learning' in recent years has brought to the fore the importance of workplace learning. In the present knowledge economy companies with highly knowledgeable...
Matt Lavin, Martin F. Wojciechowski, Peter Gasson, Colin Hughes, Elisabeth Wheeler
Morphological data and sequences from the nuclear ribosomal ITS region, and the chloroplast trnL intron and matK locus were sampled from robinioid legumes to infer phylogenetic relationships. The...
Male mating strategies and the mating system of great-tailed grackles (2000)
Johnson, Kristine, DuVal, Emily, Kielt, Megan, Hughes, Colin
Great-tailed grackles (Quiscalus mexicanus) are sexually dimorphic, dichromatic, colonially nesting blackbirds. In this study, males pursued three basic types of conditional mating strategies, each...
Koronakis, Vassilis, Cross, Michael, Hughes, Colin
The genes hly A and hly B dictating synthesis and secretion of hemolytic toxin by Escherichia coli are transcribed as part of an operon hly C,hly A,hly B but are separated by an inverted repeat and...
A Novel Membrane Protein Influencing Cell Shape and Multicellular Swarming of Proteus mirabilis
Hay, Nicole A., Tipper, Donald J., Gygi, Daniel, Hughes, Colin
Swarming in Proteus mirabilis is characterized by the coordinated surface migration of multicellular rafts of highly elongated, hyperflagellated swarm cells. We describe a transposon mutant, MNS185,...
Claret, Laurent, Hughes, Colin
The enterobacterial flhDC master operon activates expression of the flagellar biogenesis gene hierarchy and also represses cell division. During Proteus mirabilis differentiation into elongated...
Hyland, Caroline, Vuillard, Laurent, Hughes, Colin, Koronakis, Vassilis
The 1,024-amino-acid acylated hemolysin of Escherichia coli subverts host cell functions and causes cell lysis. Both activities require insertion of the toxin into target mammalian cell membranes. To...
Stanley, Peter, Koronakis, Vassilis, Hughes, Colin
The pore-forming hemolysin (HlyA) of Escherichia coli represents a unique class of bacterial toxins that require a posttranslational modification for activity. The inactive protoxin pro-HlyA is...
Transition to the open state of the TolC periplasmic tunnel entrance
Andersen, Christian, Koronakis, Eva, Bokma, Evert, Eswaran, Jeyanthy, Humphreys, Daniel, Hughes, Colin, ...
The TolC channel-tunnel spans the bacterial outer membrane and periplasm, providing a large exit duct for protein export and multidrug efflux when recruited by substrate-engaged inner membrane...
Hughes, Colin, Hacker, Jörg, Roberts, Anthony, Goebel, Werner
Potential virulence, as defined by combined levels of adhesion to urinary epithelial cells, serum resistance, and mouse toxicity, was assessed for Escherichia coli strains causing symptomatic and...
Thomas, Joanne, Stafford, Graham P., Hughes, Colin
Bacterial type III protein export underlies flagellum assembly and delivery of virulence factors into eukaryotic cells. The sequence of protein interactions underlying the export pathway are poorly...
Plasmid Carriage and the Serum Sensitivity of Enterobacteria
Taylor, Peter W., Hughes, Colin
The carriage of a range of plasmids by rough, serum-sensitive laboratory strains of Escherichia coli made no difference to their reactivity in human serum as determined by two methods....
Structure of the periplasmic component of a bacterial drug efflux pump
Higgins, Matthew K., Bokma, Evert, Koronakis, Eva, Hughes, Colin, Koronakis, Vassilis
Multidrug resistance among Gram-negative bacteria is conferred by three-component membrane pumps that expel diverse antibiotics from the cell. These efflux pumps consist of an inner membrane...
Chunnel vision: Export and efflux through bacterial channel-tunnels
Andersen, Christian, Hughes, Colin, Koronakis, Vassilis
The Escherichia coli TolC protein is central to toxin export and drug efflux across the inner and outer cell membranes and the intervening periplasmic space. The crystal structure has revealed that...
A Novel Membrane Protein Influencing Cell Shape and Multicellular Swarming of Proteus mirabilis
Hay, Nicole A., Tipper, Donald J., Gygi, Daniel, Hughes, Colin
Swarming in Proteus mirabilis is characterized by the coordinated surface migration of multicellular rafts of highly elongated, hyperflagellated swarm cells. We describe a transposon mutant, MNS185,...
Claret, Laurent, Hughes, Colin
The enterobacterial flhDC master operon activates expression of the flagellar biogenesis gene hierarchy and also represses cell division. During Proteus mirabilis differentiation into elongated...
Hyland, Caroline, Vuillard, Laurent, Hughes, Colin, Koronakis, Vassilis
The 1,024-amino-acid acylated hemolysin of Escherichia coli subverts host cell functions and causes cell lysis. Both activities require insertion of the toxin into target mammalian cell membranes. To...
Stanley, Peter, Koronakis, Vassilis, Hughes, Colin
The pore-forming hemolysin (HlyA) of Escherichia coli represents a unique class of bacterial toxins that require a posttranslational modification for activity. The inactive protoxin pro-HlyA is...
Transition to the open state of the TolC periplasmic tunnel entrance
Andersen, Christian, Koronakis, Eva, Bokma, Evert, Eswaran, Jeyanthy, Humphreys, Daniel, Hughes, Colin, ...
The TolC channel-tunnel spans the bacterial outer membrane and periplasm, providing a large exit duct for protein export and multidrug efflux when recruited by substrate-engaged inner membrane...
Hughes, Colin, Hacker, Jörg, Roberts, Anthony, Goebel, Werner
Potential virulence, as defined by combined levels of adhesion to urinary epithelial cells, serum resistance, and mouse toxicity, was assessed for Escherichia coli strains causing symptomatic and...
Thomas, Joanne, Stafford, Graham P., Hughes, Colin
Bacterial type III protein export underlies flagellum assembly and delivery of virulence factors into eukaryotic cells. The sequence of protein interactions underlying the export pathway are poorly...
Plasmid Carriage and the Serum Sensitivity of Enterobacteria
Taylor, Peter W., Hughes, Colin
The carriage of a range of plasmids by rough, serum-sensitive laboratory strains of Escherichia coli made no difference to their reactivity in human serum as determined by two methods....
Structure of the periplasmic component of a bacterial drug efflux pump
Higgins, Matthew K., Bokma, Evert, Koronakis, Eva, Hughes, Colin, Koronakis, Vassilis
Multidrug resistance among Gram-negative bacteria is conferred by three-component membrane pumps that expel diverse antibiotics from the cell. These efflux pumps consist of an inner membrane...
Chunnel vision: Export and efflux through bacterial channel-tunnels
Andersen, Christian, Hughes, Colin, Koronakis, Vassilis
The Escherichia coli TolC protein is central to toxin export and drug efflux across the inner and outer cell membranes and the intervening periplasmic space. The crystal structure has revealed that...
Species radiations provide unique insights into evolutionary processes underlying species diversification and patterns of biodiversity. To compare plant diversification over a similar time period to...
An escort mechanism for cycling of export chaperones during flagellum assembly
Evans, Lewis D. B., Stafford, Graham P., Ahmed, Sangita, Fraser, Gillian M., Hughes, Colin
Assembly of the bacterial flagellar filament requires a type III export pathway for ordered delivery of structural subunits from the cytosol to the cell surface. This is facilitated by transient...
Lobedanz, Sune, Bokma, Evert, Symmons, Martyn F., Koronakis, Eva, Hughes, Colin, Koronakis, Vassilis
Bacteria such as Escherichia coli and Pseudomonas aeruginosa expel antibiotics and other inhibitors via tripartite multidrug efflux pumps spanning the inner and outer membranes and the intervening...
The assembled structure of a complete tripartite bacterial multidrug efflux pump
Symmons, Martyn F., Bokma, Evert, Koronakis, Eva, Hughes, Colin, Koronakis, Vassilis
Bacteria like Escherichia coli and Pseudomonas aeruginosa expel drugs via tripartite multidrug efflux pumps spanning both inner and outer membranes and the intervening periplasm. In these pumps a...