Colin R. Harwood

From Gene Regulation to Gene Function: Regulatory Networks in Bacillus Subtilis (2006)

Colin R. Harwood, Ivan Moszer

Bacillus subtilis is a sporulating Gram-positive bacterium that lives primarily in the soil and associated water sources. The publication of the B. subtilis genome sequence and subsequent systematic...

From Genome to Function: Systematic Analysis of the Soil Bacterium Bacillus Subtilis (2006)

Colin R. Harwood, Samuel G. Crawshaw, Anil Wipat

Bacillus subtilis is a sporulating Gram-positive bacterium that lives primarily in the soil and associated water sources. Whilst this bacterium has been studied extensively in the laboratory,...

From Gene Regulation to Gene Function: Regulatory Networks in Bacillus subtilis (2002)

Colin R. Harwood, Ivan Moszer

Bacillus subtilis is a sporulating Gram-positive bacterium that lives primarily in the soil and associated water sources. The publication of the B. subtilis genome sequence and subsequent systematic...

From Genome to Function: Systematic Analysis of the Soil Bacterium Bacillus subtilis (2001)

Colin R. Harwood, Samuel G. Crawshaw, Anil Wipat

Bacillus subtilis is a sporulating Gram-positive bacterium that lives primarily in the soil and associated water sources. Whilst this bacterium has been studied extensively in the laboratory,...

Bacillus subtilis NhaC, an Na+/H+ Antiporter, Influences Expression of the phoPR Operon and Production of Alkaline Phosphatases

Prágai, Zoltán, Eschevins, Caroline, Bron, Sierd, Harwood, Colin R.

When Bacillus subtilis is subjected to phosphate starvation, genes of the Pho regulon are either induced or repressed. Among those induced are genes encoding alkaline phosphatases (APases). A set of...

Influence of a Cell-Wall-Associated Protease on Production of α-Amylase by Bacillus subtilis

Stephenson, Keith, Harwood, Colin R.

AmyL, an extracellular α-amylase from Bacillus licheniformis, is resistant to extracellular proteases secreted by Bacillus subtilis during growth. Nevertheless, when AmyL is produced and secreted by...

YsxC, a Putative GTP-Binding Protein Essential for Growth of Bacillus subtilis 168

Prágai, Zoltán, Harwood, Colin R.

YsxC is a member of a family of GTP-binding proteins carried by a diverse range of organisms from bacteria to yeasts, plants, and humans. To resolve the issue of whether ysxC of Bacillus subtilis is...

Optimization of the Cell Wall Microenvironment Allows Increased Production of Recombinant Bacillus anthracis Protective Antigen from B. subtilis

Thwaite, Joanne E., Baillie, Les W. J., Carter, Noel M., Stephenson, Keith, Rees, Mark, Harwood, Colin R., ...

The stability of heterologous proteins secreted by gram-positive bacteria is greatly influenced by the microenvironment on the trans side of the cytoplasmic membrane, and secreted heterologous...

Mecillinam Susceptibility of Escherichia coli K-12 Mutants Deficient in the Adenosine 3′,5′-Monophosphate System

Scott, Nigel W., Harwood, Colin R.

The mecillinam resistance of Escherichia coli K-12 mutants deficient in the enzyme responsible for the synthesis of adenosine 3′,5′-monophosphate, adenylate cyclase, has been investigated. The...

Transcriptional Regulation of the phoPR Operon in Bacillus subtilis

Prágai, Zoltán, Allenby, Nicholas E. E., O'Connor, Nicola, Dubrac, Sarah, Rapoport, Georges, Msadek, Tarek, ...

When Bacillus subtilis is subjected to phosphate starvation, the Pho regulon is activated by the PhoP-PhoR two-component signal transduction system to elicit specific responses to this nutrient...

Genome-Wide Transcriptional Analysis of the Phosphate Starvation Stimulon of Bacillus subtilis†

Allenby, Nicholas E. E., O'Connor, Nicola, Prágai, Zoltán, Ward, Alan C., Wipat, Anil, Harwood, Colin R.

Bacillus subtilis responds to phosphate starvation stress by inducing the PhoP and SigB regulons. While the PhoP regulon provides a specific response to phosphate starvation stress, maximizing the...

Phosphate Starvation Induces the Sporulation Killing Factor of Bacillus subtilis

Allenby, Nicholas E. E., Watts, Carys A., Homuth, Georg, Prágai, Zoltán, Wipat, Anil, Ward, Alan C., ...

Bacillus subtilis produces and exports a peptide sporulation killing factor (SkfA) that induces lysis of sibling cells. skfA is part of the skf operon (skfA-H), which is responsible for immunity to...

Bacillus subtilis NhaC, an Na+/H+ Antiporter, Influences Expression of the phoPR Operon and Production of Alkaline Phosphatases

Prágai, Zoltán, Eschevins, Caroline, Bron, Sierd, Harwood, Colin R.

When Bacillus subtilis is subjected to phosphate starvation, genes of the Pho regulon are either induced or repressed. Among those induced are genes encoding alkaline phosphatases (APases). A set of...

Influence of a Cell-Wall-Associated Protease on Production of α-Amylase by Bacillus subtilis

Stephenson, Keith, Harwood, Colin R.

AmyL, an extracellular α-amylase from Bacillus licheniformis, is resistant to extracellular proteases secreted by Bacillus subtilis during growth. Nevertheless, when AmyL is produced and secreted by...

YsxC, a Putative GTP-Binding Protein Essential for Growth of Bacillus subtilis 168

Prágai, Zoltán, Harwood, Colin R.

YsxC is a member of a family of GTP-binding proteins carried by a diverse range of organisms from bacteria to yeasts, plants, and humans. To resolve the issue of whether ysxC of Bacillus subtilis is...

Optimization of the Cell Wall Microenvironment Allows Increased Production of Recombinant Bacillus anthracis Protective Antigen from B. subtilis

Thwaite, Joanne E., Baillie, Les W. J., Carter, Noel M., Stephenson, Keith, Rees, Mark, Harwood, Colin R., ...

The stability of heterologous proteins secreted by gram-positive bacteria is greatly influenced by the microenvironment on the trans side of the cytoplasmic membrane, and secreted heterologous...

Mecillinam Susceptibility of Escherichia coli K-12 Mutants Deficient in the Adenosine 3′,5′-Monophosphate System

Scott, Nigel W., Harwood, Colin R.

The mecillinam resistance of Escherichia coli K-12 mutants deficient in the enzyme responsible for the synthesis of adenosine 3′,5′-monophosphate, adenylate cyclase, has been investigated. The...

Transcriptional Regulation of the phoPR Operon in Bacillus subtilis

Prágai, Zoltán, Allenby, Nicholas E. E., O'Connor, Nicola, Dubrac, Sarah, Rapoport, Georges, Msadek, Tarek, ...

When Bacillus subtilis is subjected to phosphate starvation, the Pho regulon is activated by the PhoP-PhoR two-component signal transduction system to elicit specific responses to this nutrient...

Genome-Wide Transcriptional Analysis of the Phosphate Starvation Stimulon of Bacillus subtilis†

Allenby, Nicholas E. E., O'Connor, Nicola, Prágai, Zoltán, Ward, Alan C., Wipat, Anil, Harwood, Colin R.

Bacillus subtilis responds to phosphate starvation stress by inducing the PhoP and SigB regulons. While the PhoP regulon provides a specific response to phosphate starvation stress, maximizing the...

Phosphate Starvation Induces the Sporulation Killing Factor of Bacillus subtilis

Allenby, Nicholas E. E., Watts, Carys A., Homuth, Georg, Prágai, Zoltán, Wipat, Anil, Ward, Alan C., ...

Bacillus subtilis produces and exports a peptide sporulation killing factor (SkfA) that induces lysis of sibling cells. skfA is part of the skf operon (skfA-H), which is responsible for immunity to...

From Genome to Function: Systematic Analysis of the Soil Bacterium Bacillus Subtilis

Harwood, Colin R., Crawshaw, Samuel G., Wipat, Anil

Bacillus subtilis is a sporulating Gram-positive bacterium that lives primarily in the soil and associated water sources. Whilst this bacterium has been studied extensively in the laboratory,...

From Gene Regulation to Gene Function: Regulatory Networks in Bacillus Subtilis

Harwood, Colin R., Moszer, Ivan

Bacillus subtilis is a sporulating Gram-positive bacterium that lives primarily in the soil and associated water sources. The publication of the B. subtilis genome sequence and subsequent systematic...