Aggarwal, Priya, Lessie, Matthew D., Lin, Douglas I., Pontano, Laura, Gladden, Andrew B., Nuskey, Beth, ...
Deregulation of cyclin D1 occurs in numerous human cancers through mutations, alternative splicing, and gene amplification. Although cancer-derived cyclin D1 mutants are potent oncogenes in vitro and...
Activating Notch1 mutations in mouse models of T-ALL (2005)
O'Neil, Jennifer Elinor, Calvo, Jennifer Ann, McKenna, Keith, Krishnamoorthy, Veena, Aster, Jon C., Bassing, Craig H., ...
Recent studies have demonstrated that most patients with T-cell acute lymphocytic leukemia (T-ALL) have activating mutations in NOTCH1. We sought to determine whether these mutations are also...
Mechanisms that direct ordered assembly of T cell receptor β locus V, D, and J gene segments
Sleckman, Barry P., Bassing, Craig H., Hughes, Maureen M., Okada, Ami, D'Auteuil, Margaux, Wehrly, Tara D., ...
T cell receptor (TCR) β variable region genes are assembled in progenitor T cells from germ-line Vβ, Dβ, and Jβ segments via an ordered two-step process in which Dβ to Jβ rearrangements occur...
Cloning and Functional Characterization of the Early-Lymphocyte-Specific Pb99 Gene
Sleckman, Barry P., Khan, Wasif N., Xu, Wanping, Bassing, Craig H., Malynn, Barbara A., Copeland, Neal G., ...
The Pb99 gene is specifically expressed in pre-B cells and thymocytes and not in mature B and T cells or nonlymphoid tissues, implying that it may function in early lymphoid development. We have...
Increased ionizing radiation sensitivity and genomic instability in the absence of histone H2AX
Bassing, Craig H., Chua, Katrin F., Sekiguchi, JoAnn, Suh, Heikyung, Whitlow, Scott R., Fleming, James C., ...
In mammalian cells, DNA double-strand breaks (DSBs) cause rapid phosphorylation of the H2AX core histone variant (to form γ-H2AX) in megabase chromatin domains flanking sites of DNA damage. To...
Bassing, Craig H., Tillman, Robert E., Woodman, Barbara B., Canty, David, Monroe, Robert J., Sleckman, Barry P., ...
T cell receptor (TCR) δ and α variable region genes are assembled from germ-line gene segments located in a single chromosomal locus in which TCRδ segments are situated between TCRα segments. The...
Deletion of the RAG2 C terminus leads to impaired lymphoid development in mice
Akamatsu, Yoshiko, Monroe, Robert, Dudley, Darryll D., Elkin, Sheryl K., Gärtner, Frank, Talukder, Sadiqur R., ...
The recombination-activating gene (RAG)1 and RAG2 proteins comprise the lymphocyte-specific components of the V(D)J recombinase and are required for the assembly of antigen-receptor variable-region...
Elucidation of IgH intronic enhancer functions via germ-line deletion
Perlot, Thomas, Alt, Frederick W., Bassing, Craig H., Suh, Heikyung, Pinaud, Eric
Studies of chimeric mice demonstrated that the core Ig heavy chain (IgH) intronic enhancer (iEμ) functions in V(D)J and class switch recombination at the IgH locus. To more fully evaluate the role...
53BP1 and p53 synergize to suppress genomic instability and lymphomagenesis
Morales, Julio C., Franco, Sonia, Murphy, Michael M., Bassing, Craig H., Mills, Kevin D., Adams, Melissa M., ...
p53-binding protein 1 (53BP1) participates in the cellular response to DNA double-stranded breaks where it associates with various DNA repair/cell cycle factors including the H2AX histone variant....
Mechanisms that direct ordered assembly of T cell receptor β locus V, D, and J gene segments
Sleckman, Barry P., Bassing, Craig H., Hughes, Maureen M., Okada, Ami, D'Auteuil, Margaux, Wehrly, Tara D., ...
T cell receptor (TCR) β variable region genes are assembled in progenitor T cells from germ-line Vβ, Dβ, and Jβ segments via an ordered two-step process in which Dβ to Jβ rearrangements occur...
Cloning and Functional Characterization of the Early-Lymphocyte-Specific Pb99 Gene
Sleckman, Barry P., Khan, Wasif N., Xu, Wanping, Bassing, Craig H., Malynn, Barbara A., Copeland, Neal G., ...
The Pb99 gene is specifically expressed in pre-B cells and thymocytes and not in mature B and T cells or nonlymphoid tissues, implying that it may function in early lymphoid development. We have...
Increased ionizing radiation sensitivity and genomic instability in the absence of histone H2AX
Bassing, Craig H., Chua, Katrin F., Sekiguchi, JoAnn, Suh, Heikyung, Whitlow, Scott R., Fleming, James C., ...
In mammalian cells, DNA double-strand breaks (DSBs) cause rapid phosphorylation of the H2AX core histone variant (to form γ-H2AX) in megabase chromatin domains flanking sites of DNA damage. To...
Bassing, Craig H., Tillman, Robert E., Woodman, Barbara B., Canty, David, Monroe, Robert J., Sleckman, Barry P., ...
T cell receptor (TCR) δ and α variable region genes are assembled from germ-line gene segments located in a single chromosomal locus in which TCRδ segments are situated between TCRα segments. The...
Deletion of the RAG2 C terminus leads to impaired lymphoid development in mice
Akamatsu, Yoshiko, Monroe, Robert, Dudley, Darryll D., Elkin, Sheryl K., Gärtner, Frank, Talukder, Sadiqur R., ...
The recombination-activating gene (RAG)1 and RAG2 proteins comprise the lymphocyte-specific components of the V(D)J recombinase and are required for the assembly of antigen-receptor variable-region...
Elucidation of IgH intronic enhancer functions via germ-line deletion
Perlot, Thomas, Alt, Frederick W., Bassing, Craig H., Suh, Heikyung, Pinaud, Eric
Studies of chimeric mice demonstrated that the core Ig heavy chain (IgH) intronic enhancer (iEμ) functions in V(D)J and class switch recombination at the IgH locus. To more fully evaluate the role...
53BP1 and p53 synergize to suppress genomic instability and lymphomagenesis
Morales, Julio C., Franco, Sonia, Murphy, Michael M., Bassing, Craig H., Mills, Kevin D., Adams, Melissa M., ...
p53-binding protein 1 (53BP1) participates in the cellular response to DNA double-stranded breaks where it associates with various DNA repair/cell cycle factors including the H2AX histone variant....
Activating Notch1 mutations in mouse models of T-ALL
O'Neil, Jennifer, Calvo, Jennifer, McKenna, Keith, Krishnamoorthy, Veena, Aster, Jon C., Bassing, Craig H., ...
Recent studies have demonstrated that most patients with T-cell acute lymphocytic leukemia (T-ALL) have activating mutations in NOTCH1. We sought to determine whether these mutations are also...
Wu, Cherry, Ranganath, Sheila, Gleason, Megan, Woodman, Barbara B., Borjeson, Tiffany M., Alt, Frederick W., ...
T cell antigen receptor (TCR)β V(D)J variable region exon assembly is ordered, with Dβ to Jβ rearrangements occurring before joining of Vβs to a DJβ complex. Germ-line V(D)J segments are flanked...
Aifantis, Iannis, Bassing, Craig H., Garbe, Annette I., Sawai, Katie, Alt, Frederick W., Von Boehmer, Harald
It is well established that the pre–T cell receptor for antigen (TCR) is responsible for efficient expansion and differentiation of thymocytes with productive TCRβ rearrangements. However, Ptcra-...
Defects in coding joint formation in vivo in developing ATM-deficient B and T lymphocytes
Huang, Ching-Yu, Sharma, Girdhar G., Walker, Laura M., Bassing, Craig H., Pandita, Tej K., Sleckman, Barry P.
Ataxia-telangiectasia mutated (ATM)–deficient lymphocytes exhibit defects in coding joint formation during V(D)J recombination in vitro. Similar defects in vivo should affect both T and B cell...
Ye, Qunrui, Press, Barry, Kissler, Stephan, Yang, Xiao-Feng, Lu, Linrong, Bassing, Craig H., ...
The molecular basis of CD28-dependent costimulation of T cells is poorly understood. Bcl-xγ is a member of the Bcl-x family whose expression is restricted to activated T cells and requires...
Impaired V(D)J Recombination and Lymphocyte Development in Core RAG1-expressing Mice
Dudley, Darryll D., Sekiguchi, JoAnn, Zhu, Chengming, Sadofsky, Moshe J., Whitlow, Scott, DeVido, Jeffrey, ...
RAG1 and RAG2 are the lymphocyte-specific components of the V(D)J recombinase. In vitro analyses of RAG function have relied on soluble, highly truncated “core” RAG proteins. To identify...
Aggarwal, Priya, Lessie, Matthew D., Lin, Douglas I., Pontano, Laura, Gladden, Andrew B., Nuskey, Beth, ...
Deregulation of cyclin D1 occurs in numerous human cancers through mutations, alternative splicing, and gene amplification. Although cancer-derived cyclin D1 mutants are potent oncogenes in vitro and...
Complementary functions of ATM and H2AX in development and suppression of genomic instability
Zha, Shan, Sekiguchi, JoAnn, Brush, James W., Bassing, Craig H., Alt, Frederick W.
Upon DNA damage, histone H2AX is phosphorylated by ataxia-telangiectasia mutated (ATM) and other phosphoinositide 3-kinase-related protein kinases. To elucidate further the potential overlapping and...
Bassing, Craig H., Ranganath, Sheila, Murphy, Mike, Savic, Velibor, Gleason, Meagan, Alt, Frederick W.
Histone H2AX is required to maintain genomic stability in cells and to suppress malignant transformation of lymphocytes in mice. H2ax−/−p53−/− mice succumb predominantly to immature αβ...
Pontano, Laura L., Aggarwal, Priya, Barbash, Olena, Brown, Eric J., Bassing, Craig H., Diehl, J. Alan
While mitogenic induction of cyclin D1 contributes to cell cycle progression, ubiquitin-mediated proteolysis buffers this accumulation and prevents aberrant proliferation. Because the failure to...
MRN complex function in the repair of chromosomal Rag-mediated DNA double-strand breaks
Helmink, Beth A., Bredemeyer, Andrea L., Lee, Baeck-Seung, Huang, Ching-Yu, Sharma, Girdhar G., Walker, Laura M., ...
The Mre11–Rad50–Nbs1 (MRN) complex functions in the repair of DNA double-strand breaks (DSBs) by homologous recombination (HR) at postreplicative stages of the cell cycle. During HR, the MRN...