16 S rDNA primers and the unbiased assessment of thermophile diversity (2008)
Our understanding of thermophile diversity is based predominantly on PCR studies of community DNA. ‘Universal ’ and domain-specific rRNA gene PCR primers have historically been used for the...
Enzyme stability and activity at high temperatures (2008)
Daniel, R. M., Danson, M. J., Hough, D. W., Lee, C. K., Peterson, M. E., Cowan, D. A.
Carrasco, I.J., Marquez, M.C., Xue, Y., Ma, Y., Cowan, D.A., Jones, B.E., ...
This is the authors' final draft of the paper published as International Journal of Systematic and Evolutionary Microbiology, 2007, 57(9), pp.2084-2088. The final published version is available from...
Characterisation of nitride thin films by EBSD (2002)
Trager-Cowan, C., Sweeney, F., Hastie, J., Manson-Smith, S.K., Cowan, D.A., McColl, D., ...
Thin films incorporating GaN, InGaN and AlGaN are presently arousing considerable excitement because of their suitability for UV and visible light-emitting diodes and laser diodes. However, because...
Characterisation of nitride thin films by EBSD (2002)
Trager-Cowan, C., Sweeney, F., Hastie, J., Manson-Smith, S.K., Cowan, D.A., McColl, D., ...
Thin films incorporating GaN, InGaN and AlGaN are presently arousing considerable excitement because of their suitability for UV and visible light-emitting diodes and laser diodes. However, because...
Characterisation of nitride thin films by EBSD (2002)
Trager-Cowan, C., Sweeney, F., Hastie, J., Manson-Smith, S.K., Cowan, D.A., McColl, D., ...
Thin films incorporating GaN, InGaN and AlGaN are presently arousing considerable excitement because of their suitability for UV and visible light-emitting diodes and laser diodes. However, because...
A microbiological survey of Montserrat Island hydrothermal biotopes (2000)
Atkinson, T., Gairns, S., Cowan, D. A., Danson, M. J., Hough, D. W., Johnson, D. B., ...
In March 1996, a survey of hydrothermal sites on the island of Montserrat was carried out. Six sites (Galway's Soufriere, Gages Upper and Lower Soufrieres, Hot Water Pond, Hot River, and Tar River...
Bone marrow processing and cryopreservation.
Linch, D C, Knott, L J, Patterson, K G, Cowan, D A, Harper, P G
Three different closed procedures for concentrating bone marrow progenitor cells prior to cryopreservation have been compared. These were by a manual double centrifugation method, a Hemonetics 30...
Caldolase, a chelator-insensitive extracellular serine proteinase from a Thermus spp.
Saravani, G A, Cowan, D A, Daniel, R M, Morgan, H W
An extracellular alkaline serine proteinase from Thermus strain ToK3 was isolated and purified to homogeneity by (NH4)2SO4 precipitation followed by ion-exchange chromatography on DEAE-cellulose and...
Khoo, T C, Cowan, D A, Daniel, R M, Morgan, H W
Caldolysin, the extracellular proteinase from the extreme thermophile Thermus aquaticus strain T351, is stabilized by Ca2+. A variety of metal ions were able to substitute for Ca2+. Most were unable...
Cowan, D A, Smolenski, K A, Daniel, R M, Morgan, H W
An organism growing at 88 degrees C that closely resembles Desulfurococcus mucosus produced a single extracellular proteinase. We have purified this enzyme and carried out a preliminary...
A correlation between protein thermostability and resistance to proteolysis.
Daniel, R M, Cowan, D A, Morgan, H W, Curran, M P
The loss of activity due to proteolysis of purified L-asparaginase and beta-galactosidase from different sources correlates with the thermal instability of the enzymes. A similar correlation is found...
Bone marrow processing and cryopreservation.
Linch, D C, Knott, L J, Patterson, K G, Cowan, D A, Harper, P G
Three different closed procedures for concentrating bone marrow progenitor cells prior to cryopreservation have been compared. These were by a manual double centrifugation method, a Hemonetics 30...
Caldolase, a chelator-insensitive extracellular serine proteinase from a Thermus spp.
Saravani, G A, Cowan, D A, Daniel, R M, Morgan, H W
An extracellular alkaline serine proteinase from Thermus strain ToK3 was isolated and purified to homogeneity by (NH4)2SO4 precipitation followed by ion-exchange chromatography on DEAE-cellulose and...
Khoo, T C, Cowan, D A, Daniel, R M, Morgan, H W
Caldolysin, the extracellular proteinase from the extreme thermophile Thermus aquaticus strain T351, is stabilized by Ca2+. A variety of metal ions were able to substitute for Ca2+. Most were unable...
Cowan, D A, Smolenski, K A, Daniel, R M, Morgan, H W
An organism growing at 88 degrees C that closely resembles Desulfurococcus mucosus produced a single extracellular proteinase. We have purified this enzyme and carried out a preliminary...
A correlation between protein thermostability and resistance to proteolysis.
Daniel, R M, Cowan, D A, Morgan, H W, Curran, M P
The loss of activity due to proteolysis of purified L-asparaginase and beta-galactosidase from different sources correlates with the thermal instability of the enzymes. A similar correlation is found...
Human chorionic gonadotrophin and sport.
Kicman, A T, Brooks, R V, Cowan, D A
Human chorionic gonadotrophin (hCG) is a glycoprotein hormone which is produced in large amounts during pregnancy and also by certain types of tumour. The biological action of hCG is identical to...
Criteria to indicate testosterone administration.
Kicman, A T, Brooks, R V, Collyer, S C, Cowan, D A, Nanjee, M N, Southan, G J, ...
A detection method for testosterone administration was developed using radioimmunoassay to measure the urinary ratios of testosterone (T) to epitestosterone (E) and to luteinizing hormone (LH). A...
This themed issue of the British Journal of Pharmacology has been compiled and edited by Ian McGrath, Regius Professor of Physiology at University of Glasgow and David Cowan, Director of the Drug...