Spiral Defects in Motility Assays: A Measure of Motor Protein Force (1995)
Bourdieu, L., Duke, T., Elowitz, M. B., Winkelmann, D. A., Leibler, S., Libchaber, A.
In a commonly used motility assay, cytoskeletal filaments are observed as they glide over a surface coated with motor proteins. Defects in the motion frequently interrupt the flow of filaments....
Segregated assembly of muscle myosin expressed in nonmuscle cells.
Moncman, C L, Rindt, H, Robbins, J, Winkelmann, D A
Skeletal muscle myosin cDNAs were expressed in a simian kidney cell line (COS) and a mouse myogenic cell line to investigate the mechanisms controlling early stages of myosin filament assembly. An...
Collagen fibrillogenesis in situ: fibril segments are intermediates in matrix assembly.
Birk, D E, Zycband, E I, Winkelmann, D A, Trelstad, R L
The assembly of discontinuous fibril segments and bundles was studied in 14-day chicken embryo tendons by using serial sections, transmission electron microscopy, and computer-assisted image...
Crystallization of myosin subfragment 1.
Crystals of myosin subfragment 1 from avian skeletal muscle have been grown reproducibly. They diffract x-rays to at least 4.5-A resolution. The subfragment 1 crystallizes in space group...
Winkelmann, D A, Kahan, L, Lake, J A
The location of protein S4 in the small ribosomal subunit has been identified by immunoelectron microscopy. Although intact small subunits are not reactive with antibodies directed against protein...
Motility assays using myosin attached to surfaces through specific binding to monoclonal antibodies.
Winkelmann, D A, Bourdieu, L, Kinose, F, Libchaber, A
We have analyzed the dependence of actin filament movement on the mode of myosin attachment to surfaces. Monoclonal antibodies that bind to three distinct sites were used to tether myosin to...
Flexibility of myosin attachment to surfaces influences F-actin motion.
Winkelmann, D A, Bourdieu, L, Ott, A, Kinose, F, Libchaber, A
We have analyzed the dependence of actin filament sliding movement on the mode of myosin attachment to surfaces. Monoclonal antibodies (mAbs) that bind to three distinct sites were used to tether...
Segregated assembly of muscle myosin expressed in nonmuscle cells.
Moncman, C L, Rindt, H, Robbins, J, Winkelmann, D A
Skeletal muscle myosin cDNAs were expressed in a simian kidney cell line (COS) and a mouse myogenic cell line to investigate the mechanisms controlling early stages of myosin filament assembly. An...
Collagen fibrillogenesis in situ: fibril segments are intermediates in matrix assembly.
Birk, D E, Zycband, E I, Winkelmann, D A, Trelstad, R L
The assembly of discontinuous fibril segments and bundles was studied in 14-day chicken embryo tendons by using serial sections, transmission electron microscopy, and computer-assisted image...
Crystallization of myosin subfragment 1.
Crystals of myosin subfragment 1 from avian skeletal muscle have been grown reproducibly. They diffract x-rays to at least 4.5-A resolution. The subfragment 1 crystallizes in space group...
Winkelmann, D A, Kahan, L, Lake, J A
The location of protein S4 in the small ribosomal subunit has been identified by immunoelectron microscopy. Although intact small subunits are not reactive with antibodies directed against protein...
Motility assays using myosin attached to surfaces through specific binding to monoclonal antibodies.
Winkelmann, D A, Bourdieu, L, Kinose, F, Libchaber, A
We have analyzed the dependence of actin filament movement on the mode of myosin attachment to surfaces. Monoclonal antibodies that bind to three distinct sites were used to tether myosin to...
Flexibility of myosin attachment to surfaces influences F-actin motion.
Winkelmann, D A, Bourdieu, L, Ott, A, Kinose, F, Libchaber, A
We have analyzed the dependence of actin filament sliding movement on the mode of myosin attachment to surfaces. Monoclonal antibodies (mAbs) that bind to three distinct sites were used to tether...