Xie, Zhongjian J, Singleton, P A, Bourguignon, LYW, Bikle, D D
We have previously demonstrated that phospholipase C (PLC)-gamma 1 is required for calcium-induced human keratinocyte differentiation. In the present study, we investigated whether the activation of...
Tu, Chia-Ling L, Chang, W H, Bikle, D D
Store-operated calcium entry depicts the movement of extracellular Ca2+ into cells through plasma membrane Ca2+ channels activated by depletion of intracellular Ca2+ stores. The members of the...
The role of the calcium-sensing receptor in epidermal differentiation (2004)
Tu, Chia-Ling L, Oda, Y, Komuves, L, Bikle, D D
Calcium regulates the proliferation and differentiation of keratinocytes both in vivo and in vitro. Elevated extracellular Ca2+ concentration ([Ca2+](o)) raises the intracellular free calcium...
Human keratinocyte ATP2C1 localizes to the Golgi and controls Golgi Ca2+ stores (2003)
Behne, M J, Tu, Chia-Ling L, Aronchik, I, Epstein, E, Bench, G, Bikle, D D, ...
Hailey-Hailey disease (MIM16960) is a blistering skin disease caused by mutations in the Ca2+ ATPase ATP2C1. We found that the abnormal Ca2+ signaling seen in Hailey-Hailey disease keratinocytes...
Pillai, S, Bikle, D D, Su, M J, Ratnam, A, Abe, J
1,25-Dihydroxyvitamin D3 (1,25(OH)2D3) induces the differentiation of normal human keratinocytes, in part by increasing their basal intracellular calcium levels (Cai) over a period of hours. Agonists...
The ionic control of 1,25-dihydroxyvitamin D3 production in isolated chick renal tubules.
Isolated renal tubules prepared from vitamin D-deficient chicks catalyze the 1 alpha-hydroxylation of 25-hydroxyvitamin D3 (250HD3) in vitro. The effect of calcium and phosphate on the rate of...
Bikle, D D, Murphy, E W, Rasmussen, H
Isolated kidney mitochondria prepared from Vitamin D-deficient chicks catalyze the conversion of 25-hydroxyvitamin D3 to 1,25 dihydroxyvitamin D3. It wasfound that changes in the concentrations of...
Pillai, S, Bikle, D D, Eessalu, T E, Aggarwal, B B, Elias, P M
Tumor necrosis factor alpha (TNF alpha) localizes to the epidermis when injected in vivo, but its role in the skin has heretofore not been evaluated. As a first approach to assessing the role of TNF...
Transpleural gradient of 1,25-dihydroxyvitamin D in tuberculous pleuritis.
Barnes, P F, Modlin, R L, Bikle, D D, Adams, J S
We used tuberculous pleuritis as a model to study the compartmentalization and potential immunoregulatory role of 1,25-dihydroxyvitamin D [1,25-(OH)2-D] in human granulomatous disease. In tuberculous...
1,25-Dihydroxyvitamin D3 production by human keratinocytes. Kinetics and regulation.
Bikle, D D, Nemanic, M K, Gee, E, Elias, P
Human foreskin keratinocytes in vitro metabolize 25-hydroxyvitamin D3 to a number of metabolites, including 1,25-dihydroxyvitamin D3 (1,25(OH)2D3). This metabolite remains mostly within the cell and...
Halloran, B P, Bikle, D D, Levens, M J, Castro, M E, Globus, R K, Holton, E
Administration of 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] can lower the serum concentration of 25-hydroxyvitamin (25-OH-D). To determine if 1,25(OH)2D3 lowers serum 25-OH-D by increasing clearance or...
Bikle, D D, Halloran, B P, Gee, E, Ryzen, E, Haddad, J G
We determined the free fraction of 25-dihydroxyvitamin D (25OHD) in the serum of subjects with clinical evidence of liver disease and correlated these measurements to the levels of vitamin D binding...
In previous studies we demonstrated that the biologically active vitamin D metabolite 1,25-dihydroxyvitamin D [1,25(OH)2D] increased the calmodulin (CaM) content of chick duodenal brush border...
Bikle, D D, Gee, E, Halloran, B, Haddad, J G
We measured the free concentration of 1,25-dihydroxyvitamin D (1,25[OH]2D) using centrifugal ultrafiltration, and the level of vitamin D-binding protein (DBP) in 24 normal subjects, 17 pregnant...
During wound healing, release of ATP from platelets potentially exposes the epidermis to concentrations of ATP known to alter cellular functions mediated via changes in inositol trisphosphate (IP3)...
Bikle, D D, Ratnam, A, Mauro, T, Harris, J, Pillai, S
Extracellular calcium concentrations (Cao) > 0.1 mM are required for the differentiation of normal human keratinocytes in culture. Increments in Cao result in acute and sustained increases in the...
Pillai, S, Bikle, D D, Su, M J, Ratnam, A, Abe, J
1,25-Dihydroxyvitamin D3 (1,25(OH)2D3) induces the differentiation of normal human keratinocytes, in part by increasing their basal intracellular calcium levels (Cai) over a period of hours. Agonists...
The ionic control of 1,25-dihydroxyvitamin D3 production in isolated chick renal tubules.
Isolated renal tubules prepared from vitamin D-deficient chicks catalyze the 1 alpha-hydroxylation of 25-hydroxyvitamin D3 (250HD3) in vitro. The effect of calcium and phosphate on the rate of...
Bikle, D D, Murphy, E W, Rasmussen, H
Isolated kidney mitochondria prepared from Vitamin D-deficient chicks catalyze the conversion of 25-hydroxyvitamin D3 to 1,25 dihydroxyvitamin D3. It wasfound that changes in the concentrations of...
Pillai, S, Bikle, D D, Eessalu, T E, Aggarwal, B B, Elias, P M
Tumor necrosis factor alpha (TNF alpha) localizes to the epidermis when injected in vivo, but its role in the skin has heretofore not been evaluated. As a first approach to assessing the role of TNF...
Transpleural gradient of 1,25-dihydroxyvitamin D in tuberculous pleuritis.
Barnes, P F, Modlin, R L, Bikle, D D, Adams, J S
We used tuberculous pleuritis as a model to study the compartmentalization and potential immunoregulatory role of 1,25-dihydroxyvitamin D [1,25-(OH)2-D] in human granulomatous disease. In tuberculous...
1,25-Dihydroxyvitamin D3 production by human keratinocytes. Kinetics and regulation.
Bikle, D D, Nemanic, M K, Gee, E, Elias, P
Human foreskin keratinocytes in vitro metabolize 25-hydroxyvitamin D3 to a number of metabolites, including 1,25-dihydroxyvitamin D3 (1,25(OH)2D3). This metabolite remains mostly within the cell and...
Halloran, B P, Bikle, D D, Levens, M J, Castro, M E, Globus, R K, Holton, E
Administration of 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] can lower the serum concentration of 25-hydroxyvitamin (25-OH-D). To determine if 1,25(OH)2D3 lowers serum 25-OH-D by increasing clearance or...
Bikle, D D, Halloran, B P, Gee, E, Ryzen, E, Haddad, J G
We determined the free fraction of 25-dihydroxyvitamin D (25OHD) in the serum of subjects with clinical evidence of liver disease and correlated these measurements to the levels of vitamin D binding...
In previous studies we demonstrated that the biologically active vitamin D metabolite 1,25-dihydroxyvitamin D [1,25(OH)2D] increased the calmodulin (CaM) content of chick duodenal brush border...
Bikle, D D, Gee, E, Halloran, B, Haddad, J G
We measured the free concentration of 1,25-dihydroxyvitamin D (1,25[OH]2D) using centrifugal ultrafiltration, and the level of vitamin D-binding protein (DBP) in 24 normal subjects, 17 pregnant...
During wound healing, release of ATP from platelets potentially exposes the epidermis to concentrations of ATP known to alter cellular functions mediated via changes in inositol trisphosphate (IP3)...
Bikle, D D, Ratnam, A, Mauro, T, Harris, J, Pillai, S
Extracellular calcium concentrations (Cao) > 0.1 mM are required for the differentiation of normal human keratinocytes in culture. Increments in Cao result in acute and sustained increases in the...