D. F. Klessig

Partial block to transcription of human adenovirus type 2 late genes in abortively infected monkey cells.

The multifunctional adenovirus single-strand DNA-binding protein (DBP) is highly phosphorylated. Its phosphorylation sites are located in the amino-terminal domain of the protein, and its DNA- and...

Johnston, J M, Anderson, K P, Klessig, D F

The block to efficient growth of human adenovirus in monkey cells results in depressed synthesis of late viral polypeptides. This is attributable in part to reduced steady-state levels of the...

Isolation and analysis of adenovirus type 5 mutants containing deletions in the gene encoding the DNA-binding protein.

Synthesis of the Ad2+ND5-specified 42K protein is regulated posttranscriptionally in abortively infected monkey cells.

A genetic system is described which allows the isolation and propagation of adenovirus mutants containing lesions in early region 2A (E2A), the gene encoding the multifunctional adenovirus...

Silverman, L, Anderson, K P, Klessig, D F

In abortive infections of monkey cells by Ad2+ND5, the synthesis of the simian virus 40-specific 42,000-molecular-weight (42K) protein was reduced approximately 10-fold compared with a productive...

Klockmann, U, Klessig, D F, Deppert, W

Human adenoviruses fail to multiply effectively in monkey cells. The block to the replication of these viruses can be overcome by coinfection with simian virus 40 (SV40) or when part of the SV40...

Restricted changes in the adenovirus DNA-binding protein that lead to extended host range or temperature-sensitive phenotypes.

Brough, D E, Rice, S A, Sell, S, Klessig, D F

Human adenovirus fails to multiply efficiently in monkey cells owing to a block to late viral gene expression. Ad2hr400 through Ad2hr403 are a set of host range (hr) mutants which were selected for...

Independent, spontaneous mutants of adenovirus type 2-simian virus 40 hybrid Ad2+ND3 that grow efficiently in monkey cells possess indentical mutations in the adenovirus type 2 DNA-binding protein gene.

Anderson, C W, Hardy, M M, Dunn, J J, Klessig, D F

Four independent, spontaneous mutants of the adenovirus type 2-simian virus 40 hybrid Ad2+ND3 that allow efficient growth in monkey cells were isolated previously (C. W. Anderson, Virology...

The function(s) provided by the adenovirus-specified, DNA-binding protein required for viral late gene expression is independent of the role of the protein in viral DNA replication.

Normal translation of human adenovirus mRNA in cell-free lysates prepared from abortively as well as productively infected monkey cells.

The adenovirus type 2 (Ad2) host range mutant Ad2hr400 grows efficiently in cultured monkey cells at 37 degrees C, but is cold sensitive for plaque formation and late gene expression at 32.5 degrees...

Quinlan, M P, Klessig, D F

A variety of mRNAs, including adenovirus-specified mRNAs isolated from infected human cells, were translated with similar efficiencies in S10 cell-free lysates prepared from productively and...

Proteins containing only half of the coding information of early region 1b of adenovirus are functional in human cells transformed with the herpes simplex virus type 1 thymidine kinase gene and adenovirus type 2 DNA.

Klessig, D F, Quinlan, M P, Grodzicker, T

We introduced into tk- human 143 cells adenovirus type 2 (Ad2) genes by transformation with a plasmid (p711) containing both Ad2 sequences and the herpes simplex virus type 1 (HSV-1) tk gene. p711...

Synthesis of human adenovirus early RNA species is similar in productive and abortive infections of monkey and human cells.

Identification of two nuclear subclasses of the adenovirus type 5-encoded DNA-binding protein.

Northern (RNA) blot analysis has been used to show that synthesis of early mRNA species is similar in monkey cells productively or abortively infected with human adenovirus. mRNA species from all...

Voelkerding, K, Klessig, D F

The synthesis, accumulation, and subcellular distribution of the adenovirus serotype 5 DNA-binding protein (DBP) has been examined during the infectious cycle in HeLa cells. With the onset of viral...

Nucleotide sequence of rbcL from Amaranthus hypochondriacus chloroplasts.

Michalowski, C B, Bohnert, H J, Klessig, D F, Berry, J O

Isolation and nucleotide sequence of cDNA clones for the pathogenesis-related proteins PR1a, PR1b and PR1c of Nicotiana tabacum cv. Xanthi nc induced by TMV infection.

Cutt, J R, Dixon, D C, Carr, J P, Klessig, D F

Altered mRNA splicing in monkey cells abortively infected with human adenovirus may be responsible for inefficient synthesis of the virion fiber polypeptide.

Block to multiplication of adenovirus serotype 2 in monkey cells.

Messenger RNA encoding the fiber protein of the human adenovirus serotype 2 (Ad2) capsid is inefficiently translated in abortively infected African green monkey kidney cells. The amount of fiber mRNA...

Klessig, D F, Anderson, C W

The block to adenovirus 2 (Ad2) multiplication in monkey cells can be overcome by coinfection with simian virus 40 (SV40). To identify this block we have compared the synthesis of Ad2 proteins in...

cis-acting elements and a trans-acting factor affecting alternative splicing of adenovirus L1 transcripts.

Delsert, C, Morin, N, Klessig, D F

Expression of the L1 region of adenovirus is temporally regulated by alternative splicing to yield two major RNAs encoding the 52- to 55-kilodalton (52-55K) and IIIa polypeptides. The distal acceptor...

Nuclear localization of the adenovirus DNA-binding protein: requirement for two signals and complementation during viral infection.

Synthesis and localization of pathogenesis-related proteins in tobacco.

The adenovirus DNA-binding protein (DBP) is an abundant multifunctional protein located primarily in the nuclei of infected cells. To define sequences involved in nuclear transport of DBP, a series...

Carr, J P, Dixon, D C, Nikolau, B J, Voelkerding, K V, Klessig, D F

The PR1 family of pathogenesis-related proteins from tobacco (Nicotiana tabacum L.) leaves is induced by a variety of pathogenic and chemical agents and is associated with resistance to tobacco...

Transcriptional and post-transcriptional regulation of ribulose 1,5-bisphosphate carboxylase gene expression in light- and dark-grown amaranth cotyledons.

Microinjection of mRNA enhances translational efficiency of human adenovirus fiber message in monkey cells.

The regulation of expression of the genes encoding the large subunit (LSU) and small subunit (SSU) of ribulose 1,5-bisphosphate carboxylase (RuBPCase) was examined in 1- through 8-day-old, dark-grown...

Anderson, K P, Wong, E A, Klessig, D F

In monkey cells abortively infected with human adenovirus serotype 2, the synthesis of the fiber polypeptide of the virion capsid is reduced by at least a factor of 100 when compared with that in...

Translational regulation of light-induced ribulose 1,5-bisphosphate carboxylase gene expression in amaranth.

Introduction, stable integration, and controlled expression of a chimeric adenovirus gene whose product is toxic to the recipient human cell.

The regulation of the genes encoding the large and small subunits of ribulose 1,5-bisphosphate carboxylase was examined in amaranth cotyledons in response to changes in illumination. When dark-grown...

Klessig, D F, Brough, D E, Cleghon, V

The DNA-binding protein (DBP) encoded by human adenoviruses is a multifunctional polypeptide which plays a central role in regulating the expression of the viral genes. To gain a better understanding...

Association of the adenovirus DNA-binding protein with RNA both in vitro and in vivo.

Cleghon, V G, Klessig, D F

The multifunctional DNA-binding protein (DBP) encoded by human adenovirus binds RNA. The association of purified DBP with RNA in vitro was demonstrated by using either a gel filtration or a filter...

Differential targeting of the tobacco PR-1 pathogenesis-related proteins to the extracellular space and vacuoles of crystal idioblasts.

Dixon, D C, Cutt, J R, Klessig, D F

Several biochemical and localization studies have shown that the acidic isoforms of the tobacco pathogenesis-related (PR) proteins, PR-1a, -1b and -1c are secreted to the extracellular spaces of...

Isolation of a variant of human adenovirus serotype 2 that multiplies efficiently on monkey cells.

Klessig, D F

A variant of adenovirus serotype 2 (Ad2) that overcomes the block to multiplication of the wild-type Ad2 on monkey cells is described. The variant was selected, after nitrous acid mutagenesis, by...

Characterization of a variant of human adenovirus type 2 which multiples efficiently in simian cells.

Klessig, D F, Hassell, J A

In a previous report (Klessig, J. Virol. 21:1243--1246, 1977), the isolation of a variant (H2hr400) of adenovirus serotype 2 (Ad2) that overcomes the block to multiplication of wild-type Ad2 in...

Inhibition of ascorbate peroxidase by salicylic acid and 2,6-dichloroisonicotinic acid, two inducers of plant defense responses.

Durner, J, Klessig, D F

In recent years, it has become apparent that salicylic acid (SA) plays an important role in plant defense responses to pathogen attack. Previous studies have suggested that one of SA's mechanisms of...

Two inducers of plant defense responses, 2,6-dichloroisonicotinec acid and salicylic acid, inhibit catalase activity in tobacco.

Conrath, U, Chen, Z, Ricigliano, J R, Klessig, D F

2,6-Dichloroisonicotinic acid (INA) and salicylic acid (SA) are potent inducers of plant defense responses including the synthesis of pathogenesis-related (PR) proteins and the development of...

Purification and characterization of a soluble salicylic acid-binding protein from tobacco.

Chen, Z, Ricigliano, J W, Klessig, D F

Previously, we identified a soluble salicylic acid (SA)-binding protein (SABP) in tobacco whose properties suggest that it may play a role in transmitting the SA signal during plant defense...

Identification of a soluble salicylic acid-binding protein that may function in signal transduction in the plant disease-resistance response.

Chen, Z, Klessig, D F

It has recently been demonstrated that salicylic acid (SA) may serve as an endogenous signal molecule in the induction of systemic acquired resistance in tobacco and cucumber. In addition, SA is an...

Activation of the tobacco SIP kinase by both a cell wall-derived carbohydrate elicitor and purified proteinaceous elicitins from Phytophthora spp.

Zhang, S, Du, H, Klessig, D F

Two purified proteinaceous fungal elicitors, parasiticein (an alpha elicitin) and cryptogein (a beta elicitin), as well as a fungal cell wall-derived carbohydrate elicitor all rapidly activated a...

Uncoupling PR gene expression from NPR1 and bacterial resistance: characterization of the dominant Arabidopsis cpr6-1 mutant.

Clarke, J D, Liu, Y, Klessig, D F, Dong, X

In Arabidopsis, NPR1 mediates the salicylic acid (SA)-induced expression of pathogenesis-related (PR) genes and systemic acquired resistance (SAR). Here, we report the identification of another...

PAD4 functions upstream from salicylic acid to control defense responses in Arabidopsis.

Zhou, N, Tootle, T L, Tsui, F, Klessig, D F, Glazebrook, J

The Arabidopsis PAD4 gene was previously shown to be required for synthesis of camalexin in response to infection by the virulent bacterial pathogen Pseudomonas syringae pv maculicola ES4326 but not...

Overexpression of Pto activates defense responses and confers broad resistance.

Tang, X, Xie, M, Kim, Y J, Zhou, J, Klessig, D F, Martin, G B

The tomato disease resistance (R) gene Pto specifies race-specific resistance to the bacterial pathogen Pseudomonas syringae pv tomato carrying the avrPto gene. Pto encodes a serine/threonine protein...

The Arabidopsis ssi1 mutation restores pathogenesis-related gene expression in npr1 plants and renders defensin gene expression salicylic acid dependent.

Shah, J, Kachroo, P, Klessig, D F

The Arabidopsis NPR1 gene was previously shown to be required for the salicylic acid (SA)- and benzothiadiazole (BTH)-induced expression of pathogenesis-related (PR) genes and systemic acquired...

Rapid Avr9- and Cf-9 -dependent activation of MAP kinases in tobacco cell cultures and leaves: convergence of resistance gene, elicitor, wound, and salicylate responses.

Romeis, T, Piedras, P, Zhang, S, Klessig, D F, Hirt, H, Jones, J D

The Cf-9 resistance (R) gene from tomato confers resistance to the fungal pathogen Cladosporium fulvum expressing the corresponding, pathogen-derived avirulence gene product Avr9. To understand how...

Salicylic acid activates a 48-kD MAP kinase in tobacco.

Zhang, S, Klessig, D F

The involvement of phosphorylation/dephosphorylation in the salicylic acid (SA) signal transduction pathway leading to pathogenesis-related gene induction has previously been demonstrated using...

The cpr5 mutant of Arabidopsis expresses both NPR1-dependent and NPR1-independent resistance.

Bowling, S A, Clarke, J D, Liu, Y, Klessig, D F, Dong, X

The cpr5 mutant was identified from a screen for constitutive expression of systemic acquired resistance (SAR). This single recessive mutation also leads to spontaneous expression of chlorotic...

Identification of a Soluble, High-Affinity Salicylic Acid-Binding Protein in Tobacco.

Du, H., Klessig, D. F.

Salicylic acid (SA) is a key component in the signal transduction pathway(s), leading to the activation of certain defense responses in plants after pathogen attack. Previous studies have identified...

Differential Accumulation of Salicylic Acid and Salicylic Acid-Sensitive Catalase in Different Rice Tissues.

Chen, Z., Iyer, S., Caplan, A., Klessig, D. F., Fan, B.

We previously proposed that salicylic acid (SA)-sensitive catalases serve as biological targets of SA in plant defense responses. To further examine the role of SA-sensitive catalases, we have...

Is the High Basal Level of Salicylic Acid Important for Disease Resistance in Potato?

Yu, D., Liu, Y., Fan, B., Klessig, D. F., Chen, Z.

Potato (Solanum tuberosum) plants contain a high basal level of salicylic acid (SA), the role of which in disease resistance is currently unclear. Here we report that, in spite of a drastic reduction...

A Salicylic Acid-Binding Activity and a Salicylic Acid-Inhibitable Catalase Activity Are Present in a Variety of Plant Species.

Sanchez-Casas, P., Klessig, D. F.

Recently, it has been demonstrated that the salicylic acid (SA)-binding protein (SABP) from tobacco (Nicotiana tabacum) is a SA-inhibitable catalase (Z. Chen, H. Silva, D.F. Klessig [1993] Science...

Light-mediated control of translational initiation of ribulose-1, 5-bisphosphate carboxylase in amaranth cotyledons.

Berry, J O, Breiding, D E, Klessig, D F

In cotyledons of 6-day-old amaranth seedlings, the large subunit (LSU) and the small subunit (SSU) polypeptides of ribulose-1,5-bisphosphate carboxylase are not synthesized in the absence of light....

A mutation in Arabidopsis that leads to constitutive expression of systemic acquired resistance.

Bowling, S A, Guo, A, Cao, H, Gordon, A S, Klessig, D F, Dong, X

Systemic acquired resistance (SAR) is a nonspecific defense response in plants that is associated with an increase in the endogenous level of salicylic acid (SA) and elevated expression of...

Isolation and characterization of a viable adenovirus mutant defective in nuclear transport of the DNA-binding protein.

Cleghon, V, Voelkerding, K, Morin, N, Delsert, C, Klessig, D F

The isolation and characterization of an adenovirus mutant, Ad5dl802r1, containing two independent deletions in the 72-kilodalton (kDa) DNA-binding protein (DBP) gene is described. The two deletions...

Characterization of the translational defect to fiber synthesis in monkey cells abortively infected with human adenovirus: role of ancillary leaders.

Silverman, L, Klessig, D F

Synthesis of the fiber protein of human adenovirus serotype 2 (Ad2) is 100-fold lower in abortively infected monkey cells, compared with productive infections, despite only a 5- to 10-fold reduction...

Mutations that affect phosphorylation of the adenovirus DNA-binding protein alter its ability to enhance its own synthesis.

Partial block to transcription of human adenovirus type 2 late genes in abortively infected monkey cells.

The multifunctional adenovirus single-strand DNA-binding protein (DBP) is highly phosphorylated. Its phosphorylation sites are located in the amino-terminal domain of the protein, and its DNA- and...

Johnston, J M, Anderson, K P, Klessig, D F

The block to efficient growth of human adenovirus in monkey cells results in depressed synthesis of late viral polypeptides. This is attributable in part to reduced steady-state levels of the...

Isolation and analysis of adenovirus type 5 mutants containing deletions in the gene encoding the DNA-binding protein.

Synthesis of the Ad2+ND5-specified 42K protein is regulated posttranscriptionally in abortively infected monkey cells.

A genetic system is described which allows the isolation and propagation of adenovirus mutants containing lesions in early region 2A (E2A), the gene encoding the multifunctional adenovirus...

Silverman, L, Anderson, K P, Klessig, D F

In abortive infections of monkey cells by Ad2+ND5, the synthesis of the simian virus 40-specific 42,000-molecular-weight (42K) protein was reduced approximately 10-fold compared with a productive...

Klockmann, U, Klessig, D F, Deppert, W

Human adenoviruses fail to multiply effectively in monkey cells. The block to the replication of these viruses can be overcome by coinfection with simian virus 40 (SV40) or when part of the SV40...

Restricted changes in the adenovirus DNA-binding protein that lead to extended host range or temperature-sensitive phenotypes.

Brough, D E, Rice, S A, Sell, S, Klessig, D F

Human adenovirus fails to multiply efficiently in monkey cells owing to a block to late viral gene expression. Ad2hr400 through Ad2hr403 are a set of host range (hr) mutants which were selected for...

Independent, spontaneous mutants of adenovirus type 2-simian virus 40 hybrid Ad2+ND3 that grow efficiently in monkey cells possess indentical mutations in the adenovirus type 2 DNA-binding protein gene.

Anderson, C W, Hardy, M M, Dunn, J J, Klessig, D F

Four independent, spontaneous mutants of the adenovirus type 2-simian virus 40 hybrid Ad2+ND3 that allow efficient growth in monkey cells were isolated previously (C. W. Anderson, Virology...

The function(s) provided by the adenovirus-specified, DNA-binding protein required for viral late gene expression is independent of the role of the protein in viral DNA replication.

Normal translation of human adenovirus mRNA in cell-free lysates prepared from abortively as well as productively infected monkey cells.

The adenovirus type 2 (Ad2) host range mutant Ad2hr400 grows efficiently in cultured monkey cells at 37 degrees C, but is cold sensitive for plaque formation and late gene expression at 32.5 degrees...

Quinlan, M P, Klessig, D F

A variety of mRNAs, including adenovirus-specified mRNAs isolated from infected human cells, were translated with similar efficiencies in S10 cell-free lysates prepared from productively and...

Proteins containing only half of the coding information of early region 1b of adenovirus are functional in human cells transformed with the herpes simplex virus type 1 thymidine kinase gene and adenovirus type 2 DNA.

Klessig, D F, Quinlan, M P, Grodzicker, T

We introduced into tk- human 143 cells adenovirus type 2 (Ad2) genes by transformation with a plasmid (p711) containing both Ad2 sequences and the herpes simplex virus type 1 (HSV-1) tk gene. p711...

Synthesis of human adenovirus early RNA species is similar in productive and abortive infections of monkey and human cells.

Identification of two nuclear subclasses of the adenovirus type 5-encoded DNA-binding protein.

Northern (RNA) blot analysis has been used to show that synthesis of early mRNA species is similar in monkey cells productively or abortively infected with human adenovirus. mRNA species from all...

Voelkerding, K, Klessig, D F

The synthesis, accumulation, and subcellular distribution of the adenovirus serotype 5 DNA-binding protein (DBP) has been examined during the infectious cycle in HeLa cells. With the onset of viral...

Nucleotide sequence of rbcL from Amaranthus hypochondriacus chloroplasts.

Michalowski, C B, Bohnert, H J, Klessig, D F, Berry, J O

Isolation and nucleotide sequence of cDNA clones for the pathogenesis-related proteins PR1a, PR1b and PR1c of Nicotiana tabacum cv. Xanthi nc induced by TMV infection.

Cutt, J R, Dixon, D C, Carr, J P, Klessig, D F

Altered mRNA splicing in monkey cells abortively infected with human adenovirus may be responsible for inefficient synthesis of the virion fiber polypeptide.

Block to multiplication of adenovirus serotype 2 in monkey cells.

Messenger RNA encoding the fiber protein of the human adenovirus serotype 2 (Ad2) capsid is inefficiently translated in abortively infected African green monkey kidney cells. The amount of fiber mRNA...

Klessig, D F, Anderson, C W

The block to adenovirus 2 (Ad2) multiplication in monkey cells can be overcome by coinfection with simian virus 40 (SV40). To identify this block we have compared the synthesis of Ad2 proteins in...

cis-acting elements and a trans-acting factor affecting alternative splicing of adenovirus L1 transcripts.

Delsert, C, Morin, N, Klessig, D F

Expression of the L1 region of adenovirus is temporally regulated by alternative splicing to yield two major RNAs encoding the 52- to 55-kilodalton (52-55K) and IIIa polypeptides. The distal acceptor...

Nuclear localization of the adenovirus DNA-binding protein: requirement for two signals and complementation during viral infection.

Synthesis and localization of pathogenesis-related proteins in tobacco.

The adenovirus DNA-binding protein (DBP) is an abundant multifunctional protein located primarily in the nuclei of infected cells. To define sequences involved in nuclear transport of DBP, a series...

Carr, J P, Dixon, D C, Nikolau, B J, Voelkerding, K V, Klessig, D F

The PR1 family of pathogenesis-related proteins from tobacco (Nicotiana tabacum L.) leaves is induced by a variety of pathogenic and chemical agents and is associated with resistance to tobacco...

Transcriptional and post-transcriptional regulation of ribulose 1,5-bisphosphate carboxylase gene expression in light- and dark-grown amaranth cotyledons.

Microinjection of mRNA enhances translational efficiency of human adenovirus fiber message in monkey cells.

The regulation of expression of the genes encoding the large subunit (LSU) and small subunit (SSU) of ribulose 1,5-bisphosphate carboxylase (RuBPCase) was examined in 1- through 8-day-old, dark-grown...

Anderson, K P, Wong, E A, Klessig, D F

In monkey cells abortively infected with human adenovirus serotype 2, the synthesis of the fiber polypeptide of the virion capsid is reduced by at least a factor of 100 when compared with that in...

Translational regulation of light-induced ribulose 1,5-bisphosphate carboxylase gene expression in amaranth.