Ross, J B, Senear, D F, Waxman, E, Kombo, B B, Rusinova, E, Huang, Y T, ...
We have used a tryptophan-requiring Escherichia coli auxotroph to replace the three tryptophan residues of lambda cI repressor with 5-hydroxy-L-tryptophan (5-OHTrp). By using a nonleaky promoter, we...
Flanking DNA-sequences contribute to the specific binding of cI-repressor and OR1.
Brenowitz, M, Senear, D F, Ackers, G K
The binding of cI-repressor to a series of mutant operators containing OR1 of the right operator of bacteriophage lambda was investigated. Sites OR2 and/or OR3 were inactivated by either point or...
"Footprint" titrations yield valid thermodynamic isotherms.
Brenowitz, M, Senear, D F, Shea, M A, Ackers, G K
A central issue in gene regulation is the mechanism, and biological function, of the cooperative binding of regulatory protein ligands to specific sites on DNA. To elucidate the physical-chemical...
Ross, J B, Senear, D F, Waxman, E, Kombo, B B, Rusinova, E, Huang, Y T, ...
We have used a tryptophan-requiring Escherichia coli auxotroph to replace the three tryptophan residues of lambda cI repressor with 5-hydroxy-L-tryptophan (5-OHTrp). By using a nonleaky promoter, we...
Flanking DNA-sequences contribute to the specific binding of cI-repressor and OR1.
Brenowitz, M, Senear, D F, Ackers, G K
The binding of cI-repressor to a series of mutant operators containing OR1 of the right operator of bacteriophage lambda was investigated. Sites OR2 and/or OR3 were inactivated by either point or...
"Footprint" titrations yield valid thermodynamic isotherms.
Brenowitz, M, Senear, D F, Shea, M A, Ackers, G K
A central issue in gene regulation is the mechanism, and biological function, of the cooperative binding of regulatory protein ligands to specific sites on DNA. To elucidate the physical-chemical...