A cytolysin encoded by Salmonella is required for survival within macrophages.
Libby, S J, Goebel, W, Ludwig, A, Buchmeier, N, Bowe, F, Fang, F C, ...
A Salmonella gene encoding a cytolysin has been identified by screening for hemolysis on blood agar. DNA sequence analyses together with genetic mapping in Salmonella suggest that it is unrelated to...
The alternative sigma factor katF (rpoS) regulates Salmonella virulence.
Fang, F C, Libby, S J, Buchmeier, N A, Loewen, P C, Switala, J, Harwood, J, ...
Nutrient limitation is a critical signal in Salmonella virulence gene regulation. The katF (rpoS) gene mediates the expression of the Salmonella spv plasmid virulence genes during bacterial...
Waters, V L, Hirata, K H, Pansegrau, W, Lanka, E, Guiney, D G
The IncP antibiotic-resistance plasmids transfer to a broad range of bacterial species. The RK2 origin of DNA transfer (oriT) consists of a 250-base-pair segment including the single-stranded...
Isolation and expression of the gene for a major surface protein of Giardia lamblia.
Gillin, F D, Hagblom, P, Harwood, J, Aley, S B, Reiner, D S, McCaffery, M, ...
To study the interactions between the parasitic protozoan Giardia lamblia and its environment, we have cloned the gene that encodes the two major surface-labeled trophozoite protein species. Sequence...
Cirillo, D M, Heffernan, E J, Wu, L, Harwood, J, Fierer, J, Guiney, D G
Rck is encoded on the Salmonella typhimurium virulence plasmid and is a member of a family of related 17- to 19-kDa outer membrane proteins of Enterobacteriaceae, including Ail (Yersinia...
Chen, C Y, Eckmann, L, Libby, S J, Fang, F C, Okamoto, S, Kagnoff, M F, ...
Adaptation to the intracellular environment of host cells is crucial for the pathogenesis of Salmonella infections. The alternative sigma factor RpoS is a global regulator of gene expression during...
Libby, S J, Adams, L G, Ficht, T A, Allen, C, Whitford, H A, Buchmeier, N A, ...
The pathogenic role of the spv (Salmonella plasmid virulence) genes of Salmonella dublin was determined in the natural, bovine host. Since the lack of overt signs of enteritis or enterocolitis due to...
Buchmeier, N, Bossie, S, Chen, C Y, Fang, F C, Guiney, D G, Libby, S J
Appropriate regulation of genes enables Salmonella typhimurium to adapt to the intracellular environment of the host. The Salmonella slyA gene is in a family of transcriptional regulators that may...
Cole, S P, Cirillo, D, Kagnoff, M F, Guiney, D G, Eckmann, L
Helicobacter pylori exists as an actively dividing spiral form and a nonculturable, but viable, metabolizing coccoid form. Both forms are present in the stomach, but their relative pathophysiologic...
Chen, C Y, Buchmeier, N A, Libby, S, Fang, F C, Krause, M, Guiney, D G
The plasmid virulence genes spvABCD of Salmonella spp. are regulated by SpvR and the stationary-phase sigma factor RpoS. The transcription of spv genes is induced during the post-exponential phase of...
The spv regulon of Salmonella dublin is essential for virulence in mice. SpvR, a LysR-type regulator, induces the expression of the spvABCD operon and its own expression in the stationary phase of...
Fang, F C, Chen, C Y, Guiney, D G, Xu, Y
sigma S (RpoS)-regulated lacZ transcriptional fusions in Salmonella typhimurium were identified from a MudJ transposon library by placing the rpoS gene under the control of the araBAD promoter and...
El-Gedaily, A, Paesold, G, Chen, C Y, Guiney, D G, Krause, M
The Salmonella plasmid virulence spvABCD genes are growth phase regulated and require RpoS for maximal expression in stationary phase. We identified a growth phase-independent expression of spv which...
The virulence plasmid-encoded spv regulon is essential for virulence of Salmonella dublin in mice. The spvR gene product belongs to the LysR family of transcriptional regulator proteins. SpvR induces...
Site-directed mutations in the relaxase operon of RP4.
Cole, S P, Lanka, E, Guiney, D G
Mutations were constructed by site-directed mutagenesis in the relaxase operon of the broad-host-range plasmid RP4. The mutations were constructed in smaller plasmids, recombined into the 60-kb RP4...
Lessl, M, Balzer, D, Lurz, R, Waters, V L, Guiney, D G, Lanka, E
We constructed a transfer system consisting of two compatible multicopy plasmids carrying the transfer regions Tra1 and Tra2 of the broad-host-range IncP plasmid RP4. In this system, the plasmid...
Krause, M, Fang, F C, Guiney, D G
The 80-kb plasmid pSDL2 of Salmonella dublin Lane is essential for lethal systemic infection in experimental mice. A cluster of five plasmid genes, designated spvR, spvA, spvB, spvC, and spvD, is...
Conjugal genetic elements in isolates of oral black-pigmented Bacteroides denticola (Prevotella denticola) and B. intermedius (P. intermedia) transfer tetracycline and penicillin resistance in the...
Roudier, C, Fierer, J, Guiney, D G
The spv region of the Salmonella virulence plasmids consists of five genes located on an 8-kb fragment previously shown to be essential for virulence in mice. Four structural genes, spvABCD, form an...
Waters, V L, Strack, B, Pansegrau, W, Lanka, E, Guiney, D G
Although the broad-host-range IncP plasmids can vegetatively replicate in diverse gram-negative bacteria, the development of shuttle vector systems has established that the host range for IncP...
Matthews, B G, Roudier, C, Guiney, D G
pBF4 is a 42-kb R plasmid from Bacteroides fragilis which transfers clindamycin resistance (Clr) independently of the chromosomal tetracycline resistance (Tcr) transfer element. We have found that...
The Salmonella dublin virulence plasmid pSDL2 is a low-copy-number plasmid that is highly conserved in its host. Deletion of the 8-kb EcoRI C fragment downstream of the virulence region leads to...
Detection of conjugal transfer systems in oral, black-pigmented Bacteroides spp.
Oral, black-pigmented Bacteroides spp. are important pathogens in oral anaerobic infections and dental disease. We detected conjugation systems in isolates of Bacteroides denticola and Bacteroides...
Growth regulation of a Salmonella plasmid gene essential for virulence.
Fang, F C, Krause, M, Roudier, C, Fierer, J, Guiney, D G
The Salmonella dublin plasmid gene vsdC is essential for virulence. We have constructed a vsdC-lacZ translational fusion to demonstrate that vsdC is selectively expressed during the stationary phase...
Guiney, D G, Deiss, C, Simnad, V, Yee, L, Pansegrau, W, Lanka, E
The conjugation system of the IncP alpha plasmid RK2/RP4 is encoded by transfer regions designated Tra1, Tra2, and Tra3. The Tra1 core region, cloned on plasmid pDG4 delta 22, consists of the origin...
The Bacteroides drug resistance plasmid pCP1 encodes clindamycin resistance (Clr) and a cryptic tetracycline resistance (Tcr) determinant that is expressed in Escherichia coli cells grown...
We report here a novel system for the conjugal transfer of bacterial chromosomes which utilizes the transfer origin (oriT) of plasmid RK2 cloned into transposon Tn5. Tn5 with oriT was inserted by...
Comparison of 10 IncP plasmids: homology in the regions involved in plasmid replication.
Chikami, G K, Guiney, D G, Schmidhauser, T J, Helinski, D R
We have examined the DNA homology in the replication regions of 10 IncP plasmids independently isolated from several different countries. Two regions of RK2, the best-studied plasmid of this group,...
A 4.4-megadalton penicillinase plasmid, pWD2, from Neisseria gonorrhoeae was transformed into Escherichia coli. pWD2 was efficiently mobilized by IncP plasmids in E. coli but not by Flac, R1drd-19,...
Isolation of plasmid deoxyribonucleic acid from two strains of Bacteroides.
Two clinical isolates of Bacteroides contained covalently closed circular deoxyribonucleic acid (DNA) as shown by sedimentation in an alkaline sucrose gradient, CsCl ethidium bromide equilibrium...
Roudier, C, Krause, M, Fierer, J, Guiney, D G
Large plasmids encoding important virulence properties have been found in several Salmonella serotypes. We have studied the relationship between the presence of a highly conserved 4-kilobase (kb)...
Cloning and expression of genes encoding Haemophilus somnus antigens.
Corbeil, L B, Chikami, G, Yarnall, M, Smith, J, Guiney, D G
A genomic library of Haemophilus somnus 2336, a virulent isolate from a calf with pneumonia (later used to reproduce H. somnus experimental pneumonia), was constructed in the cosmid vector pHC79. The...
Plasmid-mediated virulence in Salmonella dublin demonstrated by use of a Tn5-oriT construct.
Chikami, G K, Fierer, J, Guiney, D G
Salmonella dublin, a serotype which causes invasive disease in cattle and humans, carries a characteristic 80-kilobase plasmid (pSDL2). We were able to cure the plasmid from a strain of S. dublin....
Heffernan, E J, Wu, L, Louie, J, Okamoto, S, Fierer, J, Guiney, D G
Virulence-associated phenotypes of an outer membrane protein gene family of members of the family Enterobacteriaceae were compared by means of pBR322 constructs transformed into Escherichia coli...
Guiney, D G, Hasegawa, P, Davis, C E
A unique shuttle plasmid, pDP1, has been constructed to mediate gene transfer between Escherichia coli and the Gram-negative anaerobe Bacteroides fragilis. pDP1 contains the pBR322 replicon and the...
Location and nucleotide sequence of the transfer origin of the broad host range plasmid RK2.
The origin of plasmid DNA transfer, oriT, has been localized on RK2, a conjugative drug-resistance plasmid of the IncP group with a very broad host range in gram-negative bacteria. The transfer...
DNA repair is more important than catalase for Salmonella virulence in mice.
Buchmeier, N A, Libby, S J, Xu, Y, Loewen, P C, Switala, J, Guiney, D G, ...
Pathogenic microorganisms possess antioxidant defense mechanisms for protection from reactive oxygen metabolites such as hydrogen peroxide (H2O2), which are generated during the respiratory burst of...
Beninger, P R, Chikami, G, Tanabe, K, Roudier, C, Fierer, J, Guiney, D G
Plasmids of approximately 80 kb in size are found in nearly all clinical isolates of Salmonella dublin and are believed to be essential for virulence. We have shown previously that the 80-kb plasmid...
A cytolysin encoded by Salmonella is required for survival within macrophages.
Libby, S J, Goebel, W, Ludwig, A, Buchmeier, N, Bowe, F, Fang, F C, ...
A Salmonella gene encoding a cytolysin has been identified by screening for hemolysis on blood agar. DNA sequence analyses together with genetic mapping in Salmonella suggest that it is unrelated to...
The alternative sigma factor katF (rpoS) regulates Salmonella virulence.
Fang, F C, Libby, S J, Buchmeier, N A, Loewen, P C, Switala, J, Harwood, J, ...
Nutrient limitation is a critical signal in Salmonella virulence gene regulation. The katF (rpoS) gene mediates the expression of the Salmonella spv plasmid virulence genes during bacterial...
Waters, V L, Hirata, K H, Pansegrau, W, Lanka, E, Guiney, D G
The IncP antibiotic-resistance plasmids transfer to a broad range of bacterial species. The RK2 origin of DNA transfer (oriT) consists of a 250-base-pair segment including the single-stranded...
Isolation and expression of the gene for a major surface protein of Giardia lamblia.
Gillin, F D, Hagblom, P, Harwood, J, Aley, S B, Reiner, D S, McCaffery, M, ...
To study the interactions between the parasitic protozoan Giardia lamblia and its environment, we have cloned the gene that encodes the two major surface-labeled trophozoite protein species. Sequence...
Cirillo, D M, Heffernan, E J, Wu, L, Harwood, J, Fierer, J, Guiney, D G
Rck is encoded on the Salmonella typhimurium virulence plasmid and is a member of a family of related 17- to 19-kDa outer membrane proteins of Enterobacteriaceae, including Ail (Yersinia...
Chen, C Y, Eckmann, L, Libby, S J, Fang, F C, Okamoto, S, Kagnoff, M F, ...
Adaptation to the intracellular environment of host cells is crucial for the pathogenesis of Salmonella infections. The alternative sigma factor RpoS is a global regulator of gene expression during...
Libby, S J, Adams, L G, Ficht, T A, Allen, C, Whitford, H A, Buchmeier, N A, ...
The pathogenic role of the spv (Salmonella plasmid virulence) genes of Salmonella dublin was determined in the natural, bovine host. Since the lack of overt signs of enteritis or enterocolitis due to...
Buchmeier, N, Bossie, S, Chen, C Y, Fang, F C, Guiney, D G, Libby, S J
Appropriate regulation of genes enables Salmonella typhimurium to adapt to the intracellular environment of the host. The Salmonella slyA gene is in a family of transcriptional regulators that may...
Cole, S P, Cirillo, D, Kagnoff, M F, Guiney, D G, Eckmann, L
Helicobacter pylori exists as an actively dividing spiral form and a nonculturable, but viable, metabolizing coccoid form. Both forms are present in the stomach, but their relative pathophysiologic...
Chen, C Y, Buchmeier, N A, Libby, S, Fang, F C, Krause, M, Guiney, D G
The plasmid virulence genes spvABCD of Salmonella spp. are regulated by SpvR and the stationary-phase sigma factor RpoS. The transcription of spv genes is induced during the post-exponential phase of...
The spv regulon of Salmonella dublin is essential for virulence in mice. SpvR, a LysR-type regulator, induces the expression of the spvABCD operon and its own expression in the stationary phase of...
Fang, F C, Chen, C Y, Guiney, D G, Xu, Y
sigma S (RpoS)-regulated lacZ transcriptional fusions in Salmonella typhimurium were identified from a MudJ transposon library by placing the rpoS gene under the control of the araBAD promoter and...
El-Gedaily, A, Paesold, G, Chen, C Y, Guiney, D G, Krause, M
The Salmonella plasmid virulence spvABCD genes are growth phase regulated and require RpoS for maximal expression in stationary phase. We identified a growth phase-independent expression of spv which...
The virulence plasmid-encoded spv regulon is essential for virulence of Salmonella dublin in mice. The spvR gene product belongs to the LysR family of transcriptional regulator proteins. SpvR induces...
Site-directed mutations in the relaxase operon of RP4.
Cole, S P, Lanka, E, Guiney, D G
Mutations were constructed by site-directed mutagenesis in the relaxase operon of the broad-host-range plasmid RP4. The mutations were constructed in smaller plasmids, recombined into the 60-kb RP4...
Lessl, M, Balzer, D, Lurz, R, Waters, V L, Guiney, D G, Lanka, E
We constructed a transfer system consisting of two compatible multicopy plasmids carrying the transfer regions Tra1 and Tra2 of the broad-host-range IncP plasmid RP4. In this system, the plasmid...
Krause, M, Fang, F C, Guiney, D G
The 80-kb plasmid pSDL2 of Salmonella dublin Lane is essential for lethal systemic infection in experimental mice. A cluster of five plasmid genes, designated spvR, spvA, spvB, spvC, and spvD, is...
Conjugal genetic elements in isolates of oral black-pigmented Bacteroides denticola (Prevotella denticola) and B. intermedius (P. intermedia) transfer tetracycline and penicillin resistance in the...
Roudier, C, Fierer, J, Guiney, D G
The spv region of the Salmonella virulence plasmids consists of five genes located on an 8-kb fragment previously shown to be essential for virulence in mice. Four structural genes, spvABCD, form an...
Waters, V L, Strack, B, Pansegrau, W, Lanka, E, Guiney, D G
Although the broad-host-range IncP plasmids can vegetatively replicate in diverse gram-negative bacteria, the development of shuttle vector systems has established that the host range for IncP...
Matthews, B G, Roudier, C, Guiney, D G
pBF4 is a 42-kb R plasmid from Bacteroides fragilis which transfers clindamycin resistance (Clr) independently of the chromosomal tetracycline resistance (Tcr) transfer element. We have found that...
The Salmonella dublin virulence plasmid pSDL2 is a low-copy-number plasmid that is highly conserved in its host. Deletion of the 8-kb EcoRI C fragment downstream of the virulence region leads to...
Detection of conjugal transfer systems in oral, black-pigmented Bacteroides spp.
Oral, black-pigmented Bacteroides spp. are important pathogens in oral anaerobic infections and dental disease. We detected conjugation systems in isolates of Bacteroides denticola and Bacteroides...
Growth regulation of a Salmonella plasmid gene essential for virulence.
Fang, F C, Krause, M, Roudier, C, Fierer, J, Guiney, D G
The Salmonella dublin plasmid gene vsdC is essential for virulence. We have constructed a vsdC-lacZ translational fusion to demonstrate that vsdC is selectively expressed during the stationary phase...
Guiney, D G, Deiss, C, Simnad, V, Yee, L, Pansegrau, W, Lanka, E
The conjugation system of the IncP alpha plasmid RK2/RP4 is encoded by transfer regions designated Tra1, Tra2, and Tra3. The Tra1 core region, cloned on plasmid pDG4 delta 22, consists of the origin...
The Bacteroides drug resistance plasmid pCP1 encodes clindamycin resistance (Clr) and a cryptic tetracycline resistance (Tcr) determinant that is expressed in Escherichia coli cells grown...
We report here a novel system for the conjugal transfer of bacterial chromosomes which utilizes the transfer origin (oriT) of plasmid RK2 cloned into transposon Tn5. Tn5 with oriT was inserted by...
Comparison of 10 IncP plasmids: homology in the regions involved in plasmid replication.
Chikami, G K, Guiney, D G, Schmidhauser, T J, Helinski, D R
We have examined the DNA homology in the replication regions of 10 IncP plasmids independently isolated from several different countries. Two regions of RK2, the best-studied plasmid of this group,...
A 4.4-megadalton penicillinase plasmid, pWD2, from Neisseria gonorrhoeae was transformed into Escherichia coli. pWD2 was efficiently mobilized by IncP plasmids in E. coli but not by Flac, R1drd-19,...
Isolation of plasmid deoxyribonucleic acid from two strains of Bacteroides.
Two clinical isolates of Bacteroides contained covalently closed circular deoxyribonucleic acid (DNA) as shown by sedimentation in an alkaline sucrose gradient, CsCl ethidium bromide equilibrium...
Roudier, C, Krause, M, Fierer, J, Guiney, D G
Large plasmids encoding important virulence properties have been found in several Salmonella serotypes. We have studied the relationship between the presence of a highly conserved 4-kilobase (kb)...
Cloning and expression of genes encoding Haemophilus somnus antigens.
Corbeil, L B, Chikami, G, Yarnall, M, Smith, J, Guiney, D G
A genomic library of Haemophilus somnus 2336, a virulent isolate from a calf with pneumonia (later used to reproduce H. somnus experimental pneumonia), was constructed in the cosmid vector pHC79. The...
Plasmid-mediated virulence in Salmonella dublin demonstrated by use of a Tn5-oriT construct.
Chikami, G K, Fierer, J, Guiney, D G
Salmonella dublin, a serotype which causes invasive disease in cattle and humans, carries a characteristic 80-kilobase plasmid (pSDL2). We were able to cure the plasmid from a strain of S. dublin....
Heffernan, E J, Wu, L, Louie, J, Okamoto, S, Fierer, J, Guiney, D G
Virulence-associated phenotypes of an outer membrane protein gene family of members of the family Enterobacteriaceae were compared by means of pBR322 constructs transformed into Escherichia coli...
Guiney, D G, Hasegawa, P, Davis, C E
A unique shuttle plasmid, pDP1, has been constructed to mediate gene transfer between Escherichia coli and the Gram-negative anaerobe Bacteroides fragilis. pDP1 contains the pBR322 replicon and the...
Location and nucleotide sequence of the transfer origin of the broad host range plasmid RK2.
The origin of plasmid DNA transfer, oriT, has been localized on RK2, a conjugative drug-resistance plasmid of the IncP group with a very broad host range in gram-negative bacteria. The transfer...
DNA repair is more important than catalase for Salmonella virulence in mice.
Buchmeier, N A, Libby, S J, Xu, Y, Loewen, P C, Switala, J, Guiney, D G, ...
Pathogenic microorganisms possess antioxidant defense mechanisms for protection from reactive oxygen metabolites such as hydrogen peroxide (H2O2), which are generated during the respiratory burst of...
Beninger, P R, Chikami, G, Tanabe, K, Roudier, C, Fierer, J, Guiney, D G
Plasmids of approximately 80 kb in size are found in nearly all clinical isolates of Salmonella dublin and are believed to be essential for virulence. We have shown previously that the 80-kb plasmid...