Production and properties of a factor X-cellulose-binding domain fusion protein (1993)
Assouline, Z., Shen, H., Kilburn, D.G., Warren, R.A.J.
A fusion protein, FX–CBDCex, which comprises factor X with a cellulose-binding domain (CBDCex) fused to its C-terminus, was produced in BHK cells. It was purified from the culture medium by...
Rational design and PCR-based synthesis of an artificial Schizophyllum commune xylanase gene (1993)
Graham, Roger W., Atkinson, Tom, Kilburn, D.G., Miller, R.C., Warren, R.A.J
A synthetic gene encoding the Schizophyllum commune xylanase XynA was constructed by a novel PCR-based procedure. Threelong oligonucleotides were synthesized and used in combination with flanking PCR...
Creagh, A L, Ong, E, Jervis, E, Kilburn, D G, Haynes, C A
Isothermal titration microcalorimetry is combined with solution-depletion isotherm data to analyze the thermodynamics of binding of the cellulose-binding domain (CBD) from the beta-1,4-(exo)glucanase...
C1-Cx revisited: intramolecular synergism in a cellulase.
Din, N, Damude, H G, Gilkes, N R, Miller, R C, Warren, R A, Kilburn, D G
Endoglucanase A (CenA) from the bacterium Cellulomonas fimi is composed of a catalytic domain and a nonhydrolytic cellulose-binding domain that can function independently. The individual domains...
Comparison of a fungal (family I) and bacterial (family II) cellulose-binding domain.
Tomme, P, Driver, D P, Amandoron, E A, Miller, R C, Antony, R, Warren, J, ...
A family II cellulose-binding domain (CBD) of an exoglucanase/xylanase (Cex) from the bacterium Cellulomonas fimi was replaced with the family I CBD of cellobiohydrolase I (CbhI) from the fungus...
Tomme, P, Kwan, E, Gilkes, N R, Kilburn, D G, Warren, R A
The cenC gene, encoding beta-1,4-glucanase C (CenC) from Cellulomonas fimi, was overexpressed in Escherichia coli with a tac-based expression vector. The resulting polypeptide, with an apparent...
Moser, B, Gilkes, N R, Kilburn, D G, Warren, R A, Miller, R C
Two nonglycosylated endoglucanases which bind to Sephadex were purified from culture supernatants of Cellulomonas fimi grown on microcrystalline cellulose. Their Mrs were 120,000 and 130,000. The...
Meinke, A, Gilkes, N R, Kilburn, D G, Miller, R C, Warren, R A
Five cellulose-binding polypeptides were detected in Cellulomonas fimi culture supernatants. Two of them are CenA and CenB, endo-beta-1,4-glucanases which have been characterized previously; the...
Streptomyces lividans glycosylates the linker region of a beta-1,4-glycanase from Cellulomonas fimi.
Ong, E, Kilburn, D G, Miller, R C, Warren, R A
The beta-1,4-glycanase Cex of the gram-positive bacterium Cellulomonas fimi is a glycoprotein comprising a C-terminal cellulose-binding domain connected to an N-terminal catalytic domain by a linker...
Unusual sequence organization in CenB, an inverting endoglucanase from Cellulomonas fimi.
Meinke, A, Braun, C, Gilkes, N R, Kilburn, D G, Miller, R C, Warren, R A
The nucleotide sequence of the cenB gene was determined and used to deduce the amino acid sequence of endoglucanase B (CenB) of Cellulomonas fimi. CenB comprises 1,012 amino acids and has a molecular...
Meinke, A, Gilkes, N R, Kilburn, D G, Miller, R C, Warren, R A
Endoglucanase B (CenB) from the bacterium Cellulomonas fimi is divided into five discrete domains by linker sequences rich in proline and hydroxyamino acids (A. Meinke, C. Braun, N. R. Gilkes, D. G....
Wakarchuk, W W, Greenberg, N M, Kilburn, D G, Miller, R C, Warren, R A
The DNA sequence was determined for the cloned Agrobacterium sp. strain ATCC 21400 beta-glucosidase gene, abg. High-resolution nuclease S1 protection studies were used to map the abg mRNA 5' and 3'...
Regulation, initiation, and termination of the cenA and cex transcripts of Cellulomonas fimi.
Greenberg, N M, Warren, R A, Kilburn, D G, Miller, R C
We characterized the in vivo transcripts of two Cellulomonas fimi genes, the cenA gene, which encodes an extracellular endo-beta-1,4-glucanase (EC 3.2.1.4) and the cex gene, which encodes an...
Regulation and initiation of cenB transcripts of Cellulomonas fimi.
Greenberg, N M, Warren, R A, Kilburn, D G, Miller, R C
We characterized the in vivo transcription of the Cellulomonas fimi cenB gene, which encodes an extracellular endo-beta-1,4-glucanase (EC 3.2.1.4). By Northern blot (RNA blot) analysis, cenB mRNA was...
O'Neill, G P, Kilburn, D G, Warren, R A, Miller, R C
A recombinant exoglucanase was expressed in Escherichia coli to a level that exceeded 20% of total cellular protein. To obtain this level of overproduction, the exoglucanase gene coding sequence was...
Rational design and PCR-based synthesis of an artificial Schizophyllum commune xylanase gene.
Graham, R W, Atkinson, T, Kilburn, D G, Miller, R C, Warren, R A
A synthetic gene encoding the Schizophyllum commune xylanase XynA was constructed by a novel PCR-based procedure. Three long oligonucleotides were synthesized and used in combination with flanking...
Domains in microbial beta-1, 4-glycanases: sequence conservation, function, and enzyme families.
Gilkes, N R, Henrissat, B, Kilburn, D G, Miller, R C, Warren, R A
Several types of domain occur in beta-1, 4-glycanases. The best characterized of these are the catalytic domains and the cellulose-binding domains. The domains may be joined by linker sequences rich...
Shen, H, Gilkes, N R, Kilburn, D G, Miller, R C, Warren, R A
The gene cbhB from the cellulolytic bacterium Cellulomonas fimi encodes a polypeptide of 1090 amino acids. Cellobiohydrolase B (CbhB) is 1037 amino acids long, with a calculated molecular mass of...
The tertiary structure of a bacterial cellulase determined by small-angle X-ray-scattering analysis.
Pilz, I, Schwarz, E, Kilburn, D G, Miller, R C, Warren, R A, Gilkes, N R
CenA from Cellulomonas fimi is a beta-1,4-endoglucanase that binds tightly to cellulose. X-ray-scattering analyses show that the enzyme is tadpole-shaped: the previously identified catalytic and...
Doheny, J G, Jervis, E J, Guarna, M M, Humphries, R K, Warren, R A, Kilburn, D G
A chimaera of stem cell factor (SCF) and a cellulose-binding domain from the xylanase Cex (CBDCex) effectively immobilizes SCF on a cellulose surface. The fusion protein retains both the cytokine...
A novel mechanism of xylan binding by a lectin-like module from Streptomyces lividans xylanase 10A.
Boraston, A B, Tomme, P, Amandoron, E A, Kilburn, D G
The C-terminal module of xylanase 10A from Streptomyces lividans is a family 13 carbohydrate-binding module (CBM13). CBM13 binds mono- and oligo-saccharides with association constants of...
Boraston, A B, Warren, R A, Kilburn, D G
When produced by Pichia pastoris, three of the five Asn-Xaa-Ser/Thr sequences (corresponding to Asn-24, Asn-73 and Asn-87) in the carbohydrate-binding module CBM2a of xylanase 10A from Cellulomonas...
Creagh, A L, Ong, E, Jervis, E, Kilburn, D G, Haynes, C A
Isothermal titration microcalorimetry is combined with solution-depletion isotherm data to analyze the thermodynamics of binding of the cellulose-binding domain (CBD) from the beta-1,4-(exo)glucanase...
C1-Cx revisited: intramolecular synergism in a cellulase.
Din, N, Damude, H G, Gilkes, N R, Miller, R C, Warren, R A, Kilburn, D G
Endoglucanase A (CenA) from the bacterium Cellulomonas fimi is composed of a catalytic domain and a nonhydrolytic cellulose-binding domain that can function independently. The individual domains...
Comparison of a fungal (family I) and bacterial (family II) cellulose-binding domain.
Tomme, P, Driver, D P, Amandoron, E A, Miller, R C, Antony, R, Warren, J, ...
A family II cellulose-binding domain (CBD) of an exoglucanase/xylanase (Cex) from the bacterium Cellulomonas fimi was replaced with the family I CBD of cellobiohydrolase I (CbhI) from the fungus...
Tomme, P, Kwan, E, Gilkes, N R, Kilburn, D G, Warren, R A
The cenC gene, encoding beta-1,4-glucanase C (CenC) from Cellulomonas fimi, was overexpressed in Escherichia coli with a tac-based expression vector. The resulting polypeptide, with an apparent...
Moser, B, Gilkes, N R, Kilburn, D G, Warren, R A, Miller, R C
Two nonglycosylated endoglucanases which bind to Sephadex were purified from culture supernatants of Cellulomonas fimi grown on microcrystalline cellulose. Their Mrs were 120,000 and 130,000. The...
Meinke, A, Gilkes, N R, Kilburn, D G, Miller, R C, Warren, R A
Five cellulose-binding polypeptides were detected in Cellulomonas fimi culture supernatants. Two of them are CenA and CenB, endo-beta-1,4-glucanases which have been characterized previously; the...
Streptomyces lividans glycosylates the linker region of a beta-1,4-glycanase from Cellulomonas fimi.
Ong, E, Kilburn, D G, Miller, R C, Warren, R A
The beta-1,4-glycanase Cex of the gram-positive bacterium Cellulomonas fimi is a glycoprotein comprising a C-terminal cellulose-binding domain connected to an N-terminal catalytic domain by a linker...
Unusual sequence organization in CenB, an inverting endoglucanase from Cellulomonas fimi.
Meinke, A, Braun, C, Gilkes, N R, Kilburn, D G, Miller, R C, Warren, R A
The nucleotide sequence of the cenB gene was determined and used to deduce the amino acid sequence of endoglucanase B (CenB) of Cellulomonas fimi. CenB comprises 1,012 amino acids and has a molecular...
Meinke, A, Gilkes, N R, Kilburn, D G, Miller, R C, Warren, R A
Endoglucanase B (CenB) from the bacterium Cellulomonas fimi is divided into five discrete domains by linker sequences rich in proline and hydroxyamino acids (A. Meinke, C. Braun, N. R. Gilkes, D. G....
Wakarchuk, W W, Greenberg, N M, Kilburn, D G, Miller, R C, Warren, R A
The DNA sequence was determined for the cloned Agrobacterium sp. strain ATCC 21400 beta-glucosidase gene, abg. High-resolution nuclease S1 protection studies were used to map the abg mRNA 5' and 3'...
Regulation, initiation, and termination of the cenA and cex transcripts of Cellulomonas fimi.
Greenberg, N M, Warren, R A, Kilburn, D G, Miller, R C
We characterized the in vivo transcripts of two Cellulomonas fimi genes, the cenA gene, which encodes an extracellular endo-beta-1,4-glucanase (EC 3.2.1.4) and the cex gene, which encodes an...
Regulation and initiation of cenB transcripts of Cellulomonas fimi.
Greenberg, N M, Warren, R A, Kilburn, D G, Miller, R C
We characterized the in vivo transcription of the Cellulomonas fimi cenB gene, which encodes an extracellular endo-beta-1,4-glucanase (EC 3.2.1.4). By Northern blot (RNA blot) analysis, cenB mRNA was...
O'Neill, G P, Kilburn, D G, Warren, R A, Miller, R C
A recombinant exoglucanase was expressed in Escherichia coli to a level that exceeded 20% of total cellular protein. To obtain this level of overproduction, the exoglucanase gene coding sequence was...
Rational design and PCR-based synthesis of an artificial Schizophyllum commune xylanase gene.
Graham, R W, Atkinson, T, Kilburn, D G, Miller, R C, Warren, R A
A synthetic gene encoding the Schizophyllum commune xylanase XynA was constructed by a novel PCR-based procedure. Three long oligonucleotides were synthesized and used in combination with flanking...
Domains in microbial beta-1, 4-glycanases: sequence conservation, function, and enzyme families.
Gilkes, N R, Henrissat, B, Kilburn, D G, Miller, R C, Warren, R A
Several types of domain occur in beta-1, 4-glycanases. The best characterized of these are the catalytic domains and the cellulose-binding domains. The domains may be joined by linker sequences rich...
Shen, H, Gilkes, N R, Kilburn, D G, Miller, R C, Warren, R A
The gene cbhB from the cellulolytic bacterium Cellulomonas fimi encodes a polypeptide of 1090 amino acids. Cellobiohydrolase B (CbhB) is 1037 amino acids long, with a calculated molecular mass of...
The tertiary structure of a bacterial cellulase determined by small-angle X-ray-scattering analysis.
Pilz, I, Schwarz, E, Kilburn, D G, Miller, R C, Warren, R A, Gilkes, N R
CenA from Cellulomonas fimi is a beta-1,4-endoglucanase that binds tightly to cellulose. X-ray-scattering analyses show that the enzyme is tadpole-shaped: the previously identified catalytic and...
Doheny, J G, Jervis, E J, Guarna, M M, Humphries, R K, Warren, R A, Kilburn, D G
A chimaera of stem cell factor (SCF) and a cellulose-binding domain from the xylanase Cex (CBDCex) effectively immobilizes SCF on a cellulose surface. The fusion protein retains both the cytokine...
A novel mechanism of xylan binding by a lectin-like module from Streptomyces lividans xylanase 10A.
Boraston, A B, Tomme, P, Amandoron, E A, Kilburn, D G
The C-terminal module of xylanase 10A from Streptomyces lividans is a family 13 carbohydrate-binding module (CBM13). CBM13 binds mono- and oligo-saccharides with association constants of...
Boraston, A B, Warren, R A, Kilburn, D G
When produced by Pichia pastoris, three of the five Asn-Xaa-Ser/Thr sequences (corresponding to Asn-24, Asn-73 and Asn-87) in the carbohydrate-binding module CBM2a of xylanase 10A from Cellulomonas...
Characterization of a T-lymphocyte inhibitor in the serum of tumour-bearing mice.
Levy, J G, Smith, A G, Whitney, R B, McMaster, R, Kilburn, D G
Sera from mice with large tumours from a variety of tissue types and sources have been shown to contain substances capable of suppressing the proliferative response of normal mouse lymphocytes to...
Identity of a T-lymphocyte inhibitor with mouse immunoglobulin in the serum of tumour-bearing mice.
Levy, J G, McMaster, R, Kelly, B, Whitney, R B, Kilburn, D G
A component of the serum of tumour-bearing mice has been shown to be inhibitory to the immunological function of normal mouse T cells. This factor fractionates with monomeric immunoglobulin upon gel...
Probing the role of tryptophan residues in a cellulose-binding domain by chemical modification.
Bray, M. R., Johnson, P. E., Gilkes, N. R., McIntosh, L. P., Kilburn, D. G., Warren, R. A.
The cellulose-binding domain (CBDCex) of the mixed function glucanase-xylanase Cex from Cellulomonas fimi contains five tryptophans, two of which are located within the beta-barrel structure and...
Effect of adult thymectomy on tumour immunity in mice.
Takei, F., Levy, J. G., Kilburn, D. G.
The effect of adult thymectomy in DBA/2J mice on the in vitro response to syngeneic tumour cells was investigated. Spleen cells from adult mice which had been thymectomized 8 weeks previously...