McDonald, D, Vogelstein, B, King, MC, Kong, WK, Augustin, H, Hanahan, D, ...
Peer Reviewed
The comparative genetics and genomics of cancer: of mice and men (2003)
Hodgson, G, Hager, J, Chin, K, Lapuk, CA, Volik, S, Collins, C, ...
No abstract available.
D. Hanahan, Genomics Proteomics, Proteomics Combi-chem
Systems Biology can be used for… • Target identification • Target validation • Diagnostic and prognostic biomarkers • Disease progression models • Pharmacology and toxicology •...
McrA and McrB restriction phenotypes of some E.coli strains and implications for gene cloning (1995)
Raleigh, E. A., Murray, N. E., Revel, H., Blumenthal, R. M., Westaway, D., Reith, A. D., ...
Results in Table 1 for strain Y70 are withdrawn. All tests of the strain, both from our own collection and from isolates of the E.coli Genetic Stock Center, yield an McrA+/RgIA+ phenotype. Our tests...
McrA and McrB restriction phenotypes of some E.coli strains and implications for gene cloning (1988)
Raleigh, E.A., Murray, N.E., Revel, H., Blumenthal, R.M., Westaway, D., Reith, A.D., ...
The McrA and McrB (modified cytosine restriction) systems of E. coli interfere with incoming DNA containing methylcytosine. DNA from many organisms, including all mammalian and plant DNA, is expected...
Arbeit, J M, Howley, P M, Hanahan, D
High-risk human papillomaviruses (HPVs), including type 16, have been identified as factors in cervical carcinogenesis. However, the presence and expression of the virus per se appear to be...
Antiangiogenic therapy of transgenic mice impairs de novo tumor growth.
Parangi, S, O'Reilly, M, Christofori, G, Holmgren, L, Grosfeld, J, Folkman, J, ...
Angiogenesis is activated during multistage tumorigenesis prior to the emergence of solid tumors. Using a transgenic mouse model, we have tested the proposition that treatment with angiogenesis...
Jolicoeur, C, Hanahan, D, Smith, K M
Transgenic mice expressing T antigen (Tag) in pancreatic beta cells establish systemic tolerance toward this self-protein. The self-tolerance in two families of rat insulin promoter (RIP)-Tag mice,...
Dietrich, W F, Radany, E H, Smith, J S, Bishop, J M, Hanahan, D, Lander, E S
A genome-wide scan for loss of heterozygosity (LOH) in tumors provides a powerful route to the identification of genes involved in tumorigenesis. This approach has not previously been applied to...
Grant, S G, Jessee, J, Bloom, F R, Hanahan, D
Plasmids comprising transgene insertions in four lines of transgenic mice have been retrieved by plasmid rescue into a set of Escherichia coli strains with mutations in different members of the...
Determinants of the B-cell response against a transgenic autoantigen.
Skowronski, J, Jolicoeur, C, Alpert, S, Hanahan, D
The failure to induce self-tolerance of simian virus 40 large tumor antigen (T antigen) expressed in the pancreatic beta cells of transgenic mice results in an autoimmune response against this...
Cellular transformation and malignancy induced by ras require c-jun.
Johnson, R, Spiegelman, B, Hanahan, D, Wisdom, R
ras is an important oncogene in experimental animals and humans. In addition, activated ras proteins are potent inducers of the transcription factor AP-1, which is composed of heterodimeric complexes...
Progressive squamous epithelial neoplasia in K14-human papillomavirus type 16 transgenic mice.
Arbeit, J M, Münger, K, Howley, P M, Hanahan, D
To model human papillomavirus-induced neoplastic progression, expression of the early region of human papillomavirus type 16 (HPV16) was targeted to the basal cells of the squamous epithelium in...
Van Doren, K, Hanahan, D, Gluzman, Y
Recombinant viral genomes carrying a selectable drug resistance marker have been constructed by insertion of a hybrid gene for neomycin resistance into the helper-independent adenovirus vector, delta...
Beta-cell lines derived from transgenic mice expressing a hybrid insulin gene-oncogene.
Efrat, S, Linde, S, Kofod, H, Spector, D, Delannoy, M, Grant, S, ...
Three pancreatic beta-cell lines have been established from insulinomas derived from transgenic mice carrying a hybrid insulin-promoted simian virus 40 tumor antigen gene. The beta tumor cell (beta...
Lindgren, V, Sippola-Thiele, M, Skowronski, J, Wetzel, E, Howley, P M, Hanahan, D
In the BPV1.69 line of transgenic mice, the bovine papillomavirus type 1 genome elicits both benign dermal fibroblastic proliferation (fibromatoses) and malignant fibrosarcomas. Because these lesions...
Fine structural analysis of the chicken pro alpha 2 collagen gene.
Wozney, J, Hanahan, D, Morimoto, R, Boedtker, H, Doty, P
Forty-two kilobase pairs of cloned chicken DNA containing 80% of the pro alpha 2 (type I) collagen gene and 8 kilobase pairs of 3' flanking sequences have been isolated. Detailed analysis of these...
McrA and McrB restriction phenotypes of some E. coli strains and implications for gene cloning.
Raleigh, E A, Murray, N E, Revel, H, Blumenthal, R M, Westaway, D, Reith, A D, ...
The McrA and McrB (modified cytosine restriction) systems of E. coli interfere with incoming DNA containing methylcytosine. DNA from many organisms, including all mammalian and plant DNA, is expected...
Radvanyi, F, Christgau, S, Baekkeskov, S, Jolicoeur, C, Hanahan, D
Culturing and comparing the discrete stages of tumorigenesis provide a route to defining important components of the cancer phenotype and, in addition, present the opportunity to establish cell...
Efrat, S, Fleischer, N, Hanahan, D
Transgenic mice expressing an insulin-promoted H-ras hybrid gene in pancreatic beta cells developed beta-cell degeneration and diabetes. The disease was manifested in male mice by hyperglycemia,...
Sippola-Thiele, M, Hanahan, D, Howley, P M
Tumorigenesis of dermal fibroblasts in a line of transgenic mice carrying the BPV-1 genome was found to involve distinct proliferative stages. Cell cultures derived from normal skin, from benign...
Tal, M, Thorens, B, Surana, M, Fleischer, N, Lodish, H F, Hanahan, D, ...
High-level expression of the low-Km glucose transporter isoform GLUT-1 is characteristic of many cultured tumor and oncogene-transformed cells. In this study, we tested whether induction of GLUT-1...
Bidirectional activity of the rat insulin II 5'-flanking region in transgenic mice.
We have identified a new transcription initiation site in the 5'-flanking regulatory region of the rat insulin II gene. This site is located on the opposite strand with respect to the insulin gene...
A variant of the adenovirus type 5 genome which lacks EcoRI sites has been cloned in a bacterial plasmid after the addition of EcoRI oligonucleotide linkers to its ends. Closed circular forms of the...
Construction and characterization of a 2.5-kilobase procollagen clone.
Lehrach, H, Frischauf, A M, Hanahan, D, Wozney, J, Fuller, F, Crkvenjakov, R, ...
Recombinant bacterial plasmids have been constructed by inserting double-stranded chicken procollagen cDNA sequences linked to chemically synthesized decanucleotides containing HindIII sites into the...
Vivanco, M D, Johnson, R, Galante, P E, Hanahan, D, Yamamoto, K R
In transgenic mice harboring the bovine papillomavirus genome, fibrosarcomas arise along an experimentally accessible pathway in which normal dermal fibroblasts progress through two pre-neoplastic...
Onrust, S V, Hartl, P M, Rosen, S D, Hanahan, D
Immune surveillance depends on lymphocyte access to tissue. Lymphocytes emigrate from blood when adhesion receptors such as L-selectin and the alpha 4 beta 7 integrin on these cells bind to ligands...
Cationic liposomes target angiogenic endothelial cells in tumors and chronic inflammation in mice.
Thurston, G, McLean, J W, Rizen, M, Baluk, P, Haskell, A, Murphy, T J, ...
This study sought to determine whether angiogenic blood vessels in disease models preferentially bind and internalize cationic liposomes injected intravenously. Angiogenesis was examined in...
Efrat, S, Baekkeskov, S, Lane, D, Hanahan, D
The expression of p53 has been evaluated during oncogenesis of the pancreatic beta cells in transgenic mice harboring hybrid insulin-SV40 T antigen genes. Significant levels of p53 are detected in...
Arbeit, J M, Howley, P M, Hanahan, D
High-risk human papillomaviruses (HPVs), including type 16, have been identified as factors in cervical carcinogenesis. However, the presence and expression of the virus per se appear to be...
Antiangiogenic therapy of transgenic mice impairs de novo tumor growth.
Parangi, S, O'Reilly, M, Christofori, G, Holmgren, L, Grosfeld, J, Folkman, J, ...
Angiogenesis is activated during multistage tumorigenesis prior to the emergence of solid tumors. Using a transgenic mouse model, we have tested the proposition that treatment with angiogenesis...
Jolicoeur, C, Hanahan, D, Smith, K M
Transgenic mice expressing T antigen (Tag) in pancreatic beta cells establish systemic tolerance toward this self-protein. The self-tolerance in two families of rat insulin promoter (RIP)-Tag mice,...
Dietrich, W F, Radany, E H, Smith, J S, Bishop, J M, Hanahan, D, Lander, E S
A genome-wide scan for loss of heterozygosity (LOH) in tumors provides a powerful route to the identification of genes involved in tumorigenesis. This approach has not previously been applied to...
Grant, S G, Jessee, J, Bloom, F R, Hanahan, D
Plasmids comprising transgene insertions in four lines of transgenic mice have been retrieved by plasmid rescue into a set of Escherichia coli strains with mutations in different members of the...
Determinants of the B-cell response against a transgenic autoantigen.
Skowronski, J, Jolicoeur, C, Alpert, S, Hanahan, D
The failure to induce self-tolerance of simian virus 40 large tumor antigen (T antigen) expressed in the pancreatic beta cells of transgenic mice results in an autoimmune response against this...
Cellular transformation and malignancy induced by ras require c-jun.
Johnson, R, Spiegelman, B, Hanahan, D, Wisdom, R
ras is an important oncogene in experimental animals and humans. In addition, activated ras proteins are potent inducers of the transcription factor AP-1, which is composed of heterodimeric complexes...
Progressive squamous epithelial neoplasia in K14-human papillomavirus type 16 transgenic mice.
Arbeit, J M, Münger, K, Howley, P M, Hanahan, D
To model human papillomavirus-induced neoplastic progression, expression of the early region of human papillomavirus type 16 (HPV16) was targeted to the basal cells of the squamous epithelium in...
Van Doren, K, Hanahan, D, Gluzman, Y
Recombinant viral genomes carrying a selectable drug resistance marker have been constructed by insertion of a hybrid gene for neomycin resistance into the helper-independent adenovirus vector, delta...
Beta-cell lines derived from transgenic mice expressing a hybrid insulin gene-oncogene.
Efrat, S, Linde, S, Kofod, H, Spector, D, Delannoy, M, Grant, S, ...
Three pancreatic beta-cell lines have been established from insulinomas derived from transgenic mice carrying a hybrid insulin-promoted simian virus 40 tumor antigen gene. The beta tumor cell (beta...
Lindgren, V, Sippola-Thiele, M, Skowronski, J, Wetzel, E, Howley, P M, Hanahan, D
In the BPV1.69 line of transgenic mice, the bovine papillomavirus type 1 genome elicits both benign dermal fibroblastic proliferation (fibromatoses) and malignant fibrosarcomas. Because these lesions...
Fine structural analysis of the chicken pro alpha 2 collagen gene.
Wozney, J, Hanahan, D, Morimoto, R, Boedtker, H, Doty, P
Forty-two kilobase pairs of cloned chicken DNA containing 80% of the pro alpha 2 (type I) collagen gene and 8 kilobase pairs of 3' flanking sequences have been isolated. Detailed analysis of these...
McrA and McrB restriction phenotypes of some E. coli strains and implications for gene cloning.
Raleigh, E A, Murray, N E, Revel, H, Blumenthal, R M, Westaway, D, Reith, A D, ...
The McrA and McrB (modified cytosine restriction) systems of E. coli interfere with incoming DNA containing methylcytosine. DNA from many organisms, including all mammalian and plant DNA, is expected...
Radvanyi, F, Christgau, S, Baekkeskov, S, Jolicoeur, C, Hanahan, D
Culturing and comparing the discrete stages of tumorigenesis provide a route to defining important components of the cancer phenotype and, in addition, present the opportunity to establish cell...
Efrat, S, Fleischer, N, Hanahan, D
Transgenic mice expressing an insulin-promoted H-ras hybrid gene in pancreatic beta cells developed beta-cell degeneration and diabetes. The disease was manifested in male mice by hyperglycemia,...
Sippola-Thiele, M, Hanahan, D, Howley, P M
Tumorigenesis of dermal fibroblasts in a line of transgenic mice carrying the BPV-1 genome was found to involve distinct proliferative stages. Cell cultures derived from normal skin, from benign...
Tal, M, Thorens, B, Surana, M, Fleischer, N, Lodish, H F, Hanahan, D, ...
High-level expression of the low-Km glucose transporter isoform GLUT-1 is characteristic of many cultured tumor and oncogene-transformed cells. In this study, we tested whether induction of GLUT-1...
Bidirectional activity of the rat insulin II 5'-flanking region in transgenic mice.
We have identified a new transcription initiation site in the 5'-flanking regulatory region of the rat insulin II gene. This site is located on the opposite strand with respect to the insulin gene...
A variant of the adenovirus type 5 genome which lacks EcoRI sites has been cloned in a bacterial plasmid after the addition of EcoRI oligonucleotide linkers to its ends. Closed circular forms of the...
Construction and characterization of a 2.5-kilobase procollagen clone.
Lehrach, H, Frischauf, A M, Hanahan, D, Wozney, J, Fuller, F, Crkvenjakov, R, ...
Recombinant bacterial plasmids have been constructed by inserting double-stranded chicken procollagen cDNA sequences linked to chemically synthesized decanucleotides containing HindIII sites into the...
Vivanco, M D, Johnson, R, Galante, P E, Hanahan, D, Yamamoto, K R
In transgenic mice harboring the bovine papillomavirus genome, fibrosarcomas arise along an experimentally accessible pathway in which normal dermal fibroblasts progress through two pre-neoplastic...
Onrust, S V, Hartl, P M, Rosen, S D, Hanahan, D
Immune surveillance depends on lymphocyte access to tissue. Lymphocytes emigrate from blood when adhesion receptors such as L-selectin and the alpha 4 beta 7 integrin on these cells bind to ligands...
Cationic liposomes target angiogenic endothelial cells in tumors and chronic inflammation in mice.
Thurston, G, McLean, J W, Rizen, M, Baluk, P, Haskell, A, Murphy, T J, ...
This study sought to determine whether angiogenic blood vessels in disease models preferentially bind and internalize cationic liposomes injected intravenously. Angiogenesis was examined in...
Efrat, S, Baekkeskov, S, Lane, D, Hanahan, D
The expression of p53 has been evaluated during oncogenesis of the pancreatic beta cells in transgenic mice harboring hybrid insulin-SV40 T antigen genes. Significant levels of p53 are detected in...
Genetic predisposition and parameters of malignant progression in K14-HPV16 transgenic mice.
Coussens, L. M., Hanahan, D., Arbeit, J. M.
Reproducible multi-stage progression to invasive squamous carcinoma of the epidermis has been achieved in transgenic mice expressing the HPV16 early-region genes, including the E6/E7 oncogenes, under...
Transgenic mouse model: a new approach for the investigation of endocrine pancreatic B-cell growth.
Power, R F, Holm, R, Bishop, A E, Varndell, I M, Alpert, S, Hanahan, D, ...
The transformation and adaptation of pancreatic insulin-producing (B) cells has been studied in a transgenic mouse model using a panel of antisera recognising peptides and general neuroendocrine...
Neuroepithelial carcinomas in mice transgenic with human papillomavirus type 16 E6/E7 ORFs.
Arbeit, J. M., Münger, K., Howley, P. M., Hanahan, D.
The effect of the human papillomavirus (HPV) oncogenes E6 and E7 was examined in transgenic mice with a construct containing the human beta-actin promoter regulating HPV16 E6 and E7 open reading...
Pancreatic islets isolated from juvenile but not aging adult mice, when infected with a retrovirus carrying polyomavirus middle T oncogene, produced cell lines, mPAC, with characteristics both of...