Will diverse Tat interactions lead to novel antiretroviral drug targets? (2006)
Harrich, D., McMillan, N., Munoz, L., Apolloni, A., Meredith, L.
More than fifteen years following the description of Tat as a critical HIV gene expression regulatory protein, additional roles for Tat in HIV replication have been described, including reverse...
Kunjin virus replicon vectors for human immunodeficiency virus vaccine development (2003)
Harvey, T. J., Anraku, I., Linedale, R., Harrich, D., Mackenzie, J., Suhrbier, A., ...
We have previously demonstrated the ability of the vaccine vectors based on replicon RNA of the Australian flavivirus Kunjin (KUN) to induce protective antiviral and anticancer CD8(+) T-cell...
Background: In the presence of dNTPs, intact HIV-1 virions are capable of reverse transcribing at least part of their genome, a process known as natural endogenous reverse transcription (NERT). PCR...
Hooker, CW, Scott, J, Apolloni, A, Parry, E, Harrich, D
The tat gene is required by HIV-1 for efficient reverse transcription and this function of Tat can be distinguished from its role in transcription by RNA polymerase II using tat point mutations that...
Mechanistic aspects of HIV-1 reverse transcription initiation (2002)
During reverse transcription, the positive-strand HIV-1 RNA genome is converted into a double-stranded DNA copy which can be permanently integrated into the host cell genome. Recent analyses show...
Hill, MK, Hooker, CW, Harrich, D, Crowe, SM, Mak, J
The intracellular trafficking and subsequent incorporation of Gag-Pol into human immunodeficiency virus type 1 (HIV-1) remains poorly defined. Gag-Pol is encoded by the same mRNA as Gag and is...
Hooker, CW, Lott, WB, Harrich, D
Early HIV-1 reverse transcription can be separated into initiation and elongation phases. Here we show, using PCR analysis of negative-strand strong-stop DNA [(-)ssDNA] synthesis in intact virus,...
Hill, M. K., Hooker, C. W., Harrich, D., Crowe, S. M., Mak, J.
The intracellular trafficking and subsequent incorporation of Gag-Pol into human immunodeficiency virus type 1 (HIV-1) remains poorly defined. Gag-Pol is encoded by the same mRNA as Gag and is...
Ou, S H, Wu, F, Harrich, D, García-Martínez, L F, Gaynor, R B
Human immunodeficiency virus type 1 (HIV-1) gene expression is modulated by both viral and cellular factors. A regulatory element in the HIV-1 long terminal repeat known as TAR, which extends from...
Harrich, D, Mavankal, G, Mette-Snider, A, Gaynor, R B
The TAR element is a viral regulatory element extending from +1 to +60 in the human immunodeficiency virus type 1 (HIV-1) long terminal repeat, which is critical for activation by the transactivator...
Harrich, D, Ulich, C, Gaynor, R B
The regulation of human immunodeficiency virus type 1 (HIV-1) gene expression is dependent on the transactivator protein Tat and an RNA element extending from the transcription initiation site to +57...
Ulich, C, Harrich, D, Estes, P, Gaynor, R B
Mutation of either of two critical human immunodeficiency virus type 1 (HIV-1) regulatory proteins, Tat and Rev, results in marked defects in viral replication. Thus, inhibition of the function of...
Differential growth kinetics are exhibited by human immunodeficiency virus type 1 TAR mutants.
Harrich, D, Hsu, C, Race, E, Gaynor, R B
The human immunodeficiency virus type 1 (HIV-1) TAR element is critical for the activation of gene expression by the transactivator protein, Tat. Mutagenesis has demonstrated that a stable stem-loop...
Kornuc, M, Kliewer, S, Garcia, J, Harrich, D, Li, C, Gaynor, R
Transcription of the adenovirus early region 3 promoter is strongly induced by the adenovirus E1A protein. Previous DNase I footprinting has indicated that four regions in this promoter serve as...
Harrich, D, Garcia, J, Wu, F, Mitsuyasu, R, Gonazalez, J, Gaynor, R
Five regions of the human immunodeficiency virus type 1 (HIV-1) long terminal repeat (LTR) have been shown to be important in the transcriptional regulation of HIV in HeLa cells. These include the...
Human T-cell leukemia virus types I and II exhibit different DNase I protection patterns.
Altman, R, Harrich, D, Garcia, J A, Gaynor, R B
Human T-cell leukemia virus types I (HTLV-I) and II (HTLV-II) are human retroviruses which normally infect T-lymphoid cells. HTLV-I infection is associated with adult T-cell leukemia-lymphoma, and...
Gaynor, R B, Kuwabara, M D, Wu, F K, Garcia, J A, Harrich, D, Briskin, M, ...
The enhancer element of the human immunodeficiency virus type I (HIV-I) long terminal repeat (LTR) contains two copies of nearly identical sequences AGGGACTTTCC (3G sequence) and GGGGACTTTCC (4G...
Kornuc, M, Altman, R, Harrich, D, Garcia, J, Chao, J, Kayne, P, ...
The adenovirus early region 3 (E3) promoter is an early viral promoter which is strongly induced by the adenovirus transactivator protein E1A. DNase I footprinting with HeLa cell extracts has...
Alcamí, J, Folgueira, L, Pedraza, M A, Jacqué, J M, Bachelerie, F, ...
The role of NF-kappa B-dependent signals in activating the transcriptional activity of the HIV regulatory region (LTR) was analyzed by systematic comparison of HIV LTR activity in human CD4 T cells...
Garcia, J A, Harrich, D, Soultanakis, E, Wu, F, Mitsuyasu, R, Gaynor, R B
The human immunodeficiency virus (HIV) type 1 LTR is regulated at the transcriptional level by both cellular and viral proteins. Using HeLa cell extracts, multiple regions of the HIV LTR were found...
Wu, F K, Garcia, J A, Harrich, D, Gaynor, R B
Transcription of the human immunodeficiency virus type 1 (HIV-1) is regulated by viral proteins and cellular factors that bind to the viral long terminal repeat (LTR). At least five regions of the...
Garcia, J A, Harrich, D, Pearson, L, Mitsuyasu, R, Gaynor, R B
The transcriptional regulation of the human immunodeficiency virus (HIV) type I involves the interaction of both viral and cellular proteins. The viral protein tat is important in increasing the...
Harrich, D, Garcia, J, Mitsuyasu, R, Gaynor, R
Multiple regulatory elements in the human immunodeficiency virus long terminal repeat (HIV LTR) are required for activation of HIV gene expression. Previous transfection studies of HIV LTR constructs...
Williams, J L, Garcia, J, Harrich, D, Pearson, L, Wu, F, Gaynor, R
A primary site of infection by human adenoviruses is lymphoid cells. However, analysis of the viral control elements and the cellular factors that regulate adenoviral gene expression in lymphocytes...
Tat is required for efficient HIV-1 reverse transcription.
Harrich, D, Ulich, C, García-Martínez, L F, Gaynor, R B
The ability of human immunodeficiency virus-1 (HIV-1) to undergo efficient reverse transcription is dependent on a number of parameters. These include the binding of the tRNA(3)(Lys) to the HIV-1...
Hill, M. K., Hooker, C. W., Harrich, D., Crowe, S. M., Mak, J.
The intracellular trafficking and subsequent incorporation of Gag-Pol into human immunodeficiency virus type 1 (HIV-1) remains poorly defined. Gag-Pol is encoded by the same mRNA as Gag and is...
Ou, S H, Wu, F, Harrich, D, García-Martínez, L F, Gaynor, R B
Human immunodeficiency virus type 1 (HIV-1) gene expression is modulated by both viral and cellular factors. A regulatory element in the HIV-1 long terminal repeat known as TAR, which extends from...
Harrich, D, Mavankal, G, Mette-Snider, A, Gaynor, R B
The TAR element is a viral regulatory element extending from +1 to +60 in the human immunodeficiency virus type 1 (HIV-1) long terminal repeat, which is critical for activation by the transactivator...
Harrich, D, Ulich, C, Gaynor, R B
The regulation of human immunodeficiency virus type 1 (HIV-1) gene expression is dependent on the transactivator protein Tat and an RNA element extending from the transcription initiation site to +57...
Ulich, C, Harrich, D, Estes, P, Gaynor, R B
Mutation of either of two critical human immunodeficiency virus type 1 (HIV-1) regulatory proteins, Tat and Rev, results in marked defects in viral replication. Thus, inhibition of the function of...
Differential growth kinetics are exhibited by human immunodeficiency virus type 1 TAR mutants.
Harrich, D, Hsu, C, Race, E, Gaynor, R B
The human immunodeficiency virus type 1 (HIV-1) TAR element is critical for the activation of gene expression by the transactivator protein, Tat. Mutagenesis has demonstrated that a stable stem-loop...
Kornuc, M, Kliewer, S, Garcia, J, Harrich, D, Li, C, Gaynor, R
Transcription of the adenovirus early region 3 promoter is strongly induced by the adenovirus E1A protein. Previous DNase I footprinting has indicated that four regions in this promoter serve as...
Harrich, D, Garcia, J, Wu, F, Mitsuyasu, R, Gonazalez, J, Gaynor, R
Five regions of the human immunodeficiency virus type 1 (HIV-1) long terminal repeat (LTR) have been shown to be important in the transcriptional regulation of HIV in HeLa cells. These include the...
Human T-cell leukemia virus types I and II exhibit different DNase I protection patterns.
Altman, R, Harrich, D, Garcia, J A, Gaynor, R B
Human T-cell leukemia virus types I (HTLV-I) and II (HTLV-II) are human retroviruses which normally infect T-lymphoid cells. HTLV-I infection is associated with adult T-cell leukemia-lymphoma, and...
Gaynor, R B, Kuwabara, M D, Wu, F K, Garcia, J A, Harrich, D, Briskin, M, ...
The enhancer element of the human immunodeficiency virus type I (HIV-I) long terminal repeat (LTR) contains two copies of nearly identical sequences AGGGACTTTCC (3G sequence) and GGGGACTTTCC (4G...
Kornuc, M, Altman, R, Harrich, D, Garcia, J, Chao, J, Kayne, P, ...
The adenovirus early region 3 (E3) promoter is an early viral promoter which is strongly induced by the adenovirus transactivator protein E1A. DNase I footprinting with HeLa cell extracts has...
Alcamí, J, Folgueira, L, Pedraza, M A, Jacqué, J M, Bachelerie, F, ...
The role of NF-kappa B-dependent signals in activating the transcriptional activity of the HIV regulatory region (LTR) was analyzed by systematic comparison of HIV LTR activity in human CD4 T cells...
Garcia, J A, Harrich, D, Soultanakis, E, Wu, F, Mitsuyasu, R, Gaynor, R B
The human immunodeficiency virus (HIV) type 1 LTR is regulated at the transcriptional level by both cellular and viral proteins. Using HeLa cell extracts, multiple regions of the HIV LTR were found...
Wu, F K, Garcia, J A, Harrich, D, Gaynor, R B
Transcription of the human immunodeficiency virus type 1 (HIV-1) is regulated by viral proteins and cellular factors that bind to the viral long terminal repeat (LTR). At least five regions of the...
Garcia, J A, Harrich, D, Pearson, L, Mitsuyasu, R, Gaynor, R B
The transcriptional regulation of the human immunodeficiency virus (HIV) type I involves the interaction of both viral and cellular proteins. The viral protein tat is important in increasing the...
Harrich, D, Garcia, J, Mitsuyasu, R, Gaynor, R
Multiple regulatory elements in the human immunodeficiency virus long terminal repeat (HIV LTR) are required for activation of HIV gene expression. Previous transfection studies of HIV LTR constructs...
Williams, J L, Garcia, J, Harrich, D, Pearson, L, Wu, F, Gaynor, R
A primary site of infection by human adenoviruses is lymphoid cells. However, analysis of the viral control elements and the cellular factors that regulate adenoviral gene expression in lymphocytes...
Tat is required for efficient HIV-1 reverse transcription.
Harrich, D, Ulich, C, García-Martínez, L F, Gaynor, R B
The ability of human immunodeficiency virus-1 (HIV-1) to undergo efficient reverse transcription is dependent on a number of parameters. These include the binding of the tRNA(3)(Lys) to the HIV-1...