N.J. Winston, M.H. Johnson, J.M. McConnell, D.I. Cook, M.L. Day
Potassium channels play important roles in many cellular processes, including cell-cycle progression and cell differentiation. In the present study, we investigated the pattern of expression of the...
Nedd4-2 functionally interacts with CIC-5 (2004)
Hryciw, D. H., Ekberg, J., Lee, A., Lensink, I. L., Kumar, S., Guggino, W. B., ...
The kinase Grk2 regulates Nedd4/Nedd4-2-dependent control of epithelial Na+ channels (2004)
Dinudom, A., Fotia, A. B., Lefkowitz, R. J., Young, J. A., Kumar, S., Cook, D. I.
Nedd4-2 functionally interacts with CIC-5 (2004)
Hryciw, D. H., Ekberg, J., Lee, A., Lensink, I. L., Kumar, S., Guggino, W. B., ...
The kinase Grk2 regulates Nedd4/Nedd4-2-dependent control of epithelial Na+ channels (2004)
Dinudom, A., Fotia, A. B., Lefkowitz, R. J., Young, J. A., Kumar, S., Cook, D. I.
Fotia, A. B., Ekberg, J., Adams, D. J., Cook, D. I., Poronnik, P., Kumar, S.
Nedd4 and Nedd4-2 are ubiquitin-protein ligases known to regulate a number of membrane proteins including receptors and ion transporters. Regulation of the epithelial Na+ channel by Nedd4 and Nedd4-2...
Acute effects of parainfluenza virus on epithelial electrolyte transport (2004)
Kunzelmann, K., Konig, J., Sun, J., Markovich, D., King, N. J., Karupiah, G., ...
Parainfluenza viruses are important causes of respiratory disease in both children and adults. In particular, they are the major cause of the serious childhood illness croup...
Hryciw, D. H., Ekberg, J., Lee, A., Lensink, I. L., Kumar, S., Guggino, W. B., ...
Constitutive albumin uptake by the proximal tubule is achieved by a receptor-mediated process in which the Cl- channel, ClC-5, plays an obligate role. Here we investigated the functional interaction...
Weinhaus, A. J., Tabiin, M. T., Poronnik, P., Palma, C. A., Cook, D. I., Tuch, B. E.
Fetal pancreatic beta-cells release insulin poorly in response to glucose; however, the cellular mechanism for this is unknown. By using fura-2 to measure changes in the cytoplasmic free Ca2+...
E.A. Harding, C.A. Gibb, M.H. Johnson, D.I. Cook, M.L. Day
Intracellular pH recovery in Quackenbush Swiss mouse preimplantation embryos following acid loading was investigated under conditions of H+-monocarboxylate cotransporter inactivity. Isoform-sensitive...
Fotia, A. B., Dinudom, A., Shearwin, Keith Edward, Koch, J., Korbmacher, C., Cook, D. I., ...
The amiloride-sensitive epithelial sodium channel (ENaC) is essential for fluid and electrolyte homeostasis. ENaC consists of α, β, and γ subunits, each of which contains a PPxY motif that...
Fotia, A. B., Dinudom, A., Shearwin, Keith Edward, Koch, J., Korbmacher, C., Cook, D. I., ...
The amiloride-sensitive epithelial sodium channel (ENaC) is essential for fluid and electrolyte homeostasis. ENaC consists of α, β, and γ subunits, each of which contains a PPxY motif that...
Poronnik, P., Cummins, M. M., O'Mullane, L. M., Cook, D. I.
It is firmly established that the activation of many heptahelical receptors by extracellular agonists leads to the activation of effectors such as phospholipase Cbeta (PLCbeta), the subsequent...
Cummins, M. M., O'Mullane, L. M., Barden, J. A., Cook, D. I., Poronnik, P.
We used replication-deficient adenoviruses overexpressing antisense against G(q) class alpha-subunits to determine the roles of G(q) and G(11) in mediating M-3-receptor-coupled Ca2+ mobilization in...
Na+-H+ exchange in salivary secretory cells is controlled by an intracellular Na+ receptor (1999)
Ishibashi, H., Dinudom, A., Harvey, K. F., Kumar, S., Young, J. A., Cook, D. I.
Na+-H+ exchange in salivary secretory cells is controlled by an intracellular Na+ receptor (1999)
Ishibashi, H., Dinudom, A., Harvey, K. F., Kumar, S., Young, J. A., Cook, D. I.
Nedd4 mediates control of an epithelial Na+ channel in salivary duct cells by cytosic Na+ (1998)
Dinudom, A., Harvey, K. F., Komwatana, P., Young, J. A., Kumar, S., Cook, D. I.
Nedd4 mediates control of an epithelial Na+ channel in salivary duct cells by cytosic Na+ (1998)
Dinudom, A., Harvey, K. F., Komwatana, P., Young, J. A., Kumar, S., Cook, D. I.
Na+-H+ exchange in salivary secretory cells is controlled by an intracellular Na+ receptor
Ishibashi, H., Dinudom, A., Harvey, K. F., Kumar, S., Young, J. A., Cook, D. I.
It recently has been shown that epithelial Na+ channels are controlled by a receptor for intracellular Na+, a G protein (Go), and a ubiquitin-protein ligase (Nedd4). Furthermore, mutations in the...
Nedd4 mediates control of an epithelial Na+ channel in salivary duct cells by cytosolic Na+
Dinudom, A., Harvey, K. F., Komwatana, P., Young, J. A., Kumar, S., Cook, D. I.
Epithelial Na+ channels are expressed widely in absorptive epithelia such as the renal collecting duct and the colon and play a critical role in fluid and electrolyte homeostasis. Recent studies have...
Influenza virus inhibits amiloride-sensitive Na+ channels in respiratory epithelia
Kunzelmann, K., Beesley, A. H., King, N. J., Karupiah, G., Young, J. A., Cook, D. I.
Many pathogens causing diarrhea do so by modulating ion transport in the gut. Respiratory pathogens are similarly associated with disturbances of fluid balance in the respiratory tract, although it...
Komwatana, P, Dinudom, A, Young, J A, Cook, D I
In tight Na+-absorbing epithelial cells, the fate of Na+ entry through amiloride-sensitive apical membrane Na+ channels is matched to basolateral Na+ extrusion so that cell Na+ concentration and...
Intracellular location of catabolite activator protein of Escherichia coli.
The catabolite activator protein was assayed in extracts from the minicell-producing Escherichia coli strain P678-54. The level of catabolite activator protein was found to be the same in both parent...
A cytoplasmic cell cycle controls the activity of a K+ channel in pre-implantation mouse embryos.
Day, M L, Johnson, M H, Cook, D I
We previously have reported that the activity of a 240 pS K+ channel varies during the cell cycle in pre-implantation mouse embryos. In the present study, we show that: (i) the cycling of channel...
Dinudom, A, Komwatana, P, Young, J A, Cook, D I
1. We have previously reported that the Na+ conductance in mouse intralobular salivary duct cells is controlled by cytosolic anions, being inhibited by high cytosolic concentrations of Cl- and NO3-...
H+ transporters in the main excretory duct of the mouse mandibular salivary gland.
Chaturapanich, G, Ishibashi, H, Dinudom, A, Young, J A, Cook, D I
1. We used microspectrofluorimetry with the pH-sensitive fluoroprobe 2',7'-bis(2-carboxyethyl)-5(and-6)-carboxyfluorescein (BCECF) to study the regulation of cytosolic pH (pHi) in the isolated,...
Bicarbonate transport in sheep parotid secretory cells.
Steward, M C, Poronnik, P, Cook, D I
1. Intracellular pH (pH1) was measured by microfluorimetry in secretory endpieces isolated from sheep parotid glands and loaded with the pH-sensitive fluoroprobe 2',...
Na+-H+ exchange in salivary secretory cells is controlled by an intracellular Na+ receptor
Ishibashi, H., Dinudom, A., Harvey, K. F., Kumar, S., Young, J. A., Cook, D. I.
It recently has been shown that epithelial Na+ channels are controlled by a receptor for intracellular Na+, a G protein (Go), and a ubiquitin-protein ligase (Nedd4). Furthermore, mutations in the...
Nedd4 mediates control of an epithelial Na+ channel in salivary duct cells by cytosolic Na+
Dinudom, A., Harvey, K. F., Komwatana, P., Young, J. A., Kumar, S., Cook, D. I.
Epithelial Na+ channels are expressed widely in absorptive epithelia such as the renal collecting duct and the colon and play a critical role in fluid and electrolyte homeostasis. Recent studies have...
Influenza virus inhibits amiloride-sensitive Na+ channels in respiratory epithelia
Kunzelmann, K., Beesley, A. H., King, N. J., Karupiah, G., Young, J. A., Cook, D. I.
Many pathogens causing diarrhea do so by modulating ion transport in the gut. Respiratory pathogens are similarly associated with disturbances of fluid balance in the respiratory tract, although it...
Komwatana, P, Dinudom, A, Young, J A, Cook, D I
In tight Na+-absorbing epithelial cells, the fate of Na+ entry through amiloride-sensitive apical membrane Na+ channels is matched to basolateral Na+ extrusion so that cell Na+ concentration and...
Intracellular location of catabolite activator protein of Escherichia coli.
The catabolite activator protein was assayed in extracts from the minicell-producing Escherichia coli strain P678-54. The level of catabolite activator protein was found to be the same in both parent...
Dinudom, A, Komwatana, P, Young, J A, Cook, D I
1. We have previously reported that the Na+ conductance in mouse intralobular salivary duct cells is controlled by cytosolic anions, being inhibited by high cytosolic concentrations of Cl- and NO3-...
H+ transporters in the main excretory duct of the mouse mandibular salivary gland.
Chaturapanich, G, Ishibashi, H, Dinudom, A, Young, J A, Cook, D I
1. We used microspectrofluorimetry with the pH-sensitive fluoroprobe 2',7'-bis(2-carboxyethyl)-5(and-6)-carboxyfluorescein (BCECF) to study the regulation of cytosolic pH (pHi) in the isolated,...
Bicarbonate transport in sheep parotid secretory cells.
Steward, M C, Poronnik, P, Cook, D I
1. Intracellular pH (pH1) was measured by microfluorimetry in secretory endpieces isolated from sheep parotid glands and loaded with the pH-sensitive fluoroprobe 2',...
A cytoplasmic cell cycle controls the activity of a K+ channel in pre-implantation mouse embryos.
Day, M L, Johnson, M H, Cook, D I
We previously have reported that the activity of a 240 pS K+ channel varies during the cell cycle in pre-implantation mouse embryos. In the present study, we show that: (i) the cycling of channel...
A nucleotide receptor that mobilizes Ca2+ in the mouse submandibular salivary cell line ST885.
Gibb, C. A., Singh, S., Cook, D. I., Poronnik, P., Conigrave, A. D.
1. We have identified a Ca(2+)-mobilizing receptor that responds to extracellular ATP (0.1-1000 microM) in a salivary epithelial cell line (ST885). The activated receptor also stimulates Ca2+ entry...