A nonswarming mutant of Proteus mirabilis lacks the Lrp global transcriptional regulator.
Hay, N A, Tipper, D J, Gygi, D, Hughes, C
Proteus swarming is the rapid cyclical population migration across surfaces by elongated cells that hyperexpress flagellar and virulence genes. The mini-Tn5 transposon mutant mns2 was isolated as a...
Antibody specific to the 12,200-dalton spore coat protein of Bacillus subtilis was used to detect the synthesis of cross-reacting material during sporulation. Cross-reacting protein was first...
Yuan, K, Johnson, W C, Tipper, D J, Setlow, P
Several properties of the major proteins degraded during germination of spores of Bacillus cereus, Bacillus megaterium, and Bacillus subtilis have been compared. All of the proteins had low molecular...
Acid-soluble spore proteins of Bacillus subtilis.
Acid-soluble spore proteins (ASSPs) comprise about 5% of the total protein of mature spores of different Bacillus subtilis strains. They consist of three abundant species, alpha, beta, and gamma,...
Erythromycin-resistant (Eryr) mutants of Bacillus subtilis 168 fail to sporulate at high temperature (47 degrees C) but sporulate normally at 30 to 35 degrees C. They also fail to sporulate at any...
Location of peptidoglycan lytic enzymes in Bacillus sphaericus.
Guinand, M, Vacheron, M J, Michel, G, Tipper, D J
The level of three peptidoglycan hydrolases was determined in the mother cell compartment and forespores of Bacillus sphaericus. Vegetative and sporulating cells contained in LD-carboxypeptidase...
Morphology of an Escherichia coli mutant with a temperature-dependent round cell shape.
Iwaya, M, Goldman, R, Tipper, D J, Feingold, B, Strominger, J L
Mutants of Escherichia coli capable of growing in the presence of 10 microgram of mecillinam per ml were selected after intensive mutagenesis. Of these mutants, 1.4% formed normal, rod-shaped cells...
Transcriptional control of synthesis of acid-soluble proteins in sporulating Bacillus subtilis.
Johnson, W C, Mahler, I, Phillips, K, Tipper, D J
The major acid-soluble spore proteins (ASSPs) isolated from mature spores of Bacillus subtilis are designated alpha, beta, and gamma (about 60, 60, and 100 amino acids in length, respectively). Alpha...
Bacillus subtilis spore coats: complexity and purification of a unique polypeptide component.
Extensively washed, dormant spores of Bacillus subtilis were disrupted with glass beads in buffer at pH 7 in the presence of protease inhibitors. Approximately 31% of the total spore protein was...
Sporulating cells of Bacillus sphaericus 9602 containing fully engulfed forespores at different stages of maturity were broken by ultrasonic disruption, followed by grinding with alumina. In this way...
Synthesis of enzymes functional in the synthesis of nucleotide precursors of peptidoglycan ceases upon initiation of sporulation in Bacillus sphaericus. During sporulation, two periods of synthesis...
Guinand, M., Michel, G., Tipper, D. J.
Particulate preparations from sporulating cells of Bacillus sphaericus 9602 contained an endopeptidase activity that hydrolyzed the γ-d-glutamyl-(l)meso-diaminopimelic acid linkages found in the...
Ultrastructural studies of sporulation in Bacillus sphaericus.
Holt, S C, Gauther, J J, Tipper, D J
Spore septum formation in Bacillus sphaericus 9602 occurs 2 h after the end of exponential growth at one end of the vegetative cell, which retains a uniform diameter. The apparently rigid spore...
Inhibition of Yeast Ribonucleic Acid Polymerases by Thiolutin
Yeast ribonucleic acid (RNA) polymerase II, isolated after fractionation on diethylaminoethyl (DEAE)-cellulose (DE-52) or on DEAE-Sephadex (A-25), is 50% inhibited by 1.5 μg of α-amanitin. This...
Conditional Mutants of Staphylococcus aureus Defective in Cell Wall Precursor Synthesis1
Temperature-sensitive (ts) mutants of Staphylococcus aureus with defective cell wall biosynthesis have been differentiated from other ts mutants by their ability to grow at the restrictive...
Encapsidation of yeast killer double-stranded ribonucleic acids: dependence of M on L.
Bostian, K A, Sturgeon, J A, Tipper, D J
Virus-like particles containing either L or M double-stranded ribonucleic acid (dsRNA) were isolated from a killer toxin-producing strains of Saccharomyces cerevisiae (K+ R+). At least 95% of M- and...
Hanes, S D, Burn, V E, Sturley, S L, Tipper, D J, Bostian, K A
The type I killer strains of Saccharomyces cerevisiae secrete a dimeric 19-kDa protein that kills sensitive cells by disrupting cytoplasmic membrane function. This toxin is encoded by the...
Bostian, K A, Burn, V E, Jayachandran, S, Tipper, D J
In vivo transcripts of the L (4.5 kb) and M (1.9 kb) dsRNA plasmids were examined in type I killers of Saccharomyces cerevisiae. Transcripts for both plasmids include full-length (l,m) and...
Gene fusions were constructed between Ste2, the receptor for the Saccharomyces cerevisiae alpha-factor, and beta la, the secreted form of beta-lactamase encoded by the bla gene of pBR322. The Ste2...
Kang, Y S, Kane, J, Kurjan, J, Stadel, J M, Tipper, D J
Scg1, the product of the Saccharomyces cerevisiae SCG1 (also called GPA1) gene, is homologous to the alpha subunits of G proteins involved in signal transduction in mammalian cells. Scg1 negatively...
K28, a unique double-stranded RNA killer virus of Saccharomyces cerevisiae.
The double-stranded RNA (dsRNA) viruses of Saccharomyces cerevisiae consist of 4.5-kilobase-pair (kb) L species and 1.7- to 2.1-kb M species, both found in cytoplasmic viruslike particles (VLPs). The...
El-Sherbeini, M, Tipper, D J, Mitchell, D J, Bostian, K A
The plasmid determinants of killer phenotypes in type K1 and K2 killer yeast cells are the 1.9-kilobase (kb) M1 and 1.7-kb M2 double-stranded RNAs (dsRNAs), respectively. These are dependent for...
Secretion of Saccharomyces cerevisiae Killer Toxin: Processing of the Glycosylated Precursor
Bussey, H., Saville, D., Greene, D., Tipper, D. J., Bostian, K. A.
Killer toxin secretion was blocked at the restrictive temperature in Saccharomyces cerevisiae sec mutants with conditional defects in the S. cerevisiae secretory pathway leading to accumulation of...
Mode of Action of Thiolutin, an Inhibitor of Macromolecular Synthesis in Saccharomyces cerevisiae
Jimenez, A., Tipper, D. J., Davies, J.
The sulfur-containing antibiotic thiolutin has been shown to be a potent, reversible inhibitor of the growth of Saccharomyces cerevisiae. Viability was unaffected over the concentration range of 4 to...
In Vivo Effect of Thiolutin on Cell Growth and Macromolecular Synthesis in Escherichia coli
Khachatourians, G. G., Tipper, D. J.
Thiolutin reversibly inhibits growth and ribonucleic acid synthesis in Saccharomyces cerevisiae. It is now demonstrated that, at 5 μg/ml, thiolutin rapidly inhibits all incorporation of radioactive...
Mapping of functional domains within the Saccharomyces cerevisiae type 1 killer preprotoxin.
Sturley, S L, Elliot, Q, LeVitre, J, Tipper, D J, Bostian, K A
Strains of Saccharomyces cerevisiae harboring M1-dsRNA, the determinant of type 1 killer and immunity phenotypes, secrete a dimeric 19-kd toxin that kills sensitive yeast cells by the production of...
A nonswarming mutant of Proteus mirabilis lacks the Lrp global transcriptional regulator.
Hay, N A, Tipper, D J, Gygi, D, Hughes, C
Proteus swarming is the rapid cyclical population migration across surfaces by elongated cells that hyperexpress flagellar and virulence genes. The mini-Tn5 transposon mutant mns2 was isolated as a...
Antibody specific to the 12,200-dalton spore coat protein of Bacillus subtilis was used to detect the synthesis of cross-reacting material during sporulation. Cross-reacting protein was first...
Yuan, K, Johnson, W C, Tipper, D J, Setlow, P
Several properties of the major proteins degraded during germination of spores of Bacillus cereus, Bacillus megaterium, and Bacillus subtilis have been compared. All of the proteins had low molecular...
Acid-soluble spore proteins of Bacillus subtilis.
Acid-soluble spore proteins (ASSPs) comprise about 5% of the total protein of mature spores of different Bacillus subtilis strains. They consist of three abundant species, alpha, beta, and gamma,...
Erythromycin-resistant (Eryr) mutants of Bacillus subtilis 168 fail to sporulate at high temperature (47 degrees C) but sporulate normally at 30 to 35 degrees C. They also fail to sporulate at any...
Location of peptidoglycan lytic enzymes in Bacillus sphaericus.
Guinand, M, Vacheron, M J, Michel, G, Tipper, D J
The level of three peptidoglycan hydrolases was determined in the mother cell compartment and forespores of Bacillus sphaericus. Vegetative and sporulating cells contained in LD-carboxypeptidase...
Morphology of an Escherichia coli mutant with a temperature-dependent round cell shape.
Iwaya, M, Goldman, R, Tipper, D J, Feingold, B, Strominger, J L
Mutants of Escherichia coli capable of growing in the presence of 10 microgram of mecillinam per ml were selected after intensive mutagenesis. Of these mutants, 1.4% formed normal, rod-shaped cells...
Transcriptional control of synthesis of acid-soluble proteins in sporulating Bacillus subtilis.
Johnson, W C, Mahler, I, Phillips, K, Tipper, D J
The major acid-soluble spore proteins (ASSPs) isolated from mature spores of Bacillus subtilis are designated alpha, beta, and gamma (about 60, 60, and 100 amino acids in length, respectively). Alpha...
Bacillus subtilis spore coats: complexity and purification of a unique polypeptide component.
Extensively washed, dormant spores of Bacillus subtilis were disrupted with glass beads in buffer at pH 7 in the presence of protease inhibitors. Approximately 31% of the total spore protein was...
Sporulating cells of Bacillus sphaericus 9602 containing fully engulfed forespores at different stages of maturity were broken by ultrasonic disruption, followed by grinding with alumina. In this way...
Synthesis of enzymes functional in the synthesis of nucleotide precursors of peptidoglycan ceases upon initiation of sporulation in Bacillus sphaericus. During sporulation, two periods of synthesis...
Guinand, M., Michel, G., Tipper, D. J.
Particulate preparations from sporulating cells of Bacillus sphaericus 9602 contained an endopeptidase activity that hydrolyzed the γ-d-glutamyl-(l)meso-diaminopimelic acid linkages found in the...
Ultrastructural studies of sporulation in Bacillus sphaericus.
Holt, S C, Gauther, J J, Tipper, D J
Spore septum formation in Bacillus sphaericus 9602 occurs 2 h after the end of exponential growth at one end of the vegetative cell, which retains a uniform diameter. The apparently rigid spore...
Inhibition of Yeast Ribonucleic Acid Polymerases by Thiolutin
Yeast ribonucleic acid (RNA) polymerase II, isolated after fractionation on diethylaminoethyl (DEAE)-cellulose (DE-52) or on DEAE-Sephadex (A-25), is 50% inhibited by 1.5 μg of α-amanitin. This...
Conditional Mutants of Staphylococcus aureus Defective in Cell Wall Precursor Synthesis1
Temperature-sensitive (ts) mutants of Staphylococcus aureus with defective cell wall biosynthesis have been differentiated from other ts mutants by their ability to grow at the restrictive...
Encapsidation of yeast killer double-stranded ribonucleic acids: dependence of M on L.
Bostian, K A, Sturgeon, J A, Tipper, D J
Virus-like particles containing either L or M double-stranded ribonucleic acid (dsRNA) were isolated from a killer toxin-producing strains of Saccharomyces cerevisiae (K+ R+). At least 95% of M- and...
Hanes, S D, Burn, V E, Sturley, S L, Tipper, D J, Bostian, K A
The type I killer strains of Saccharomyces cerevisiae secrete a dimeric 19-kDa protein that kills sensitive cells by disrupting cytoplasmic membrane function. This toxin is encoded by the...
Bostian, K A, Burn, V E, Jayachandran, S, Tipper, D J
In vivo transcripts of the L (4.5 kb) and M (1.9 kb) dsRNA plasmids were examined in type I killers of Saccharomyces cerevisiae. Transcripts for both plasmids include full-length (l,m) and...
Gene fusions were constructed between Ste2, the receptor for the Saccharomyces cerevisiae alpha-factor, and beta la, the secreted form of beta-lactamase encoded by the bla gene of pBR322. The Ste2...
Kang, Y S, Kane, J, Kurjan, J, Stadel, J M, Tipper, D J
Scg1, the product of the Saccharomyces cerevisiae SCG1 (also called GPA1) gene, is homologous to the alpha subunits of G proteins involved in signal transduction in mammalian cells. Scg1 negatively...
K28, a unique double-stranded RNA killer virus of Saccharomyces cerevisiae.
The double-stranded RNA (dsRNA) viruses of Saccharomyces cerevisiae consist of 4.5-kilobase-pair (kb) L species and 1.7- to 2.1-kb M species, both found in cytoplasmic viruslike particles (VLPs). The...
El-Sherbeini, M, Tipper, D J, Mitchell, D J, Bostian, K A
The plasmid determinants of killer phenotypes in type K1 and K2 killer yeast cells are the 1.9-kilobase (kb) M1 and 1.7-kb M2 double-stranded RNAs (dsRNAs), respectively. These are dependent for...
Secretion of Saccharomyces cerevisiae Killer Toxin: Processing of the Glycosylated Precursor
Bussey, H., Saville, D., Greene, D., Tipper, D. J., Bostian, K. A.
Killer toxin secretion was blocked at the restrictive temperature in Saccharomyces cerevisiae sec mutants with conditional defects in the S. cerevisiae secretory pathway leading to accumulation of...
Mode of Action of Thiolutin, an Inhibitor of Macromolecular Synthesis in Saccharomyces cerevisiae
Jimenez, A., Tipper, D. J., Davies, J.
The sulfur-containing antibiotic thiolutin has been shown to be a potent, reversible inhibitor of the growth of Saccharomyces cerevisiae. Viability was unaffected over the concentration range of 4 to...
In Vivo Effect of Thiolutin on Cell Growth and Macromolecular Synthesis in Escherichia coli
Khachatourians, G. G., Tipper, D. J.
Thiolutin reversibly inhibits growth and ribonucleic acid synthesis in Saccharomyces cerevisiae. It is now demonstrated that, at 5 μg/ml, thiolutin rapidly inhibits all incorporation of radioactive...
Mapping of functional domains within the Saccharomyces cerevisiae type 1 killer preprotoxin.
Sturley, S L, Elliot, Q, LeVitre, J, Tipper, D J, Bostian, K A
Strains of Saccharomyces cerevisiae harboring M1-dsRNA, the determinant of type 1 killer and immunity phenotypes, secrete a dimeric 19-kd toxin that kills sensitive yeast cells by the production of...