Topography of a vacuolar-type H+-translocating ATPase: chromaffin-granule membrane ATPase I.
Apps, D K, Percy, J M, Perez-Castineira, J R
Proteins exposed on the cytoplasmic face of isolated chromaffin granules were labelled by lactoperoxidase-catalysed radioiodination and by non-enzymic biotinylation. Granule membranes were then...
The proton-translocating adenosine triphosphatase (ATPase) of bovine chromaffin granules contains up to five different polypeptides. Its activity is inhibited by N-ethylmaleimide, and ATP protects...
Perez-Castiñeira, J R, Apps, D K
A procedure has been developed for the rapid purification and reconstitution into phospholipid vesicles of the proton-translocating ATPase of bovine adrenal chromaffin-granule membranes. It involves...
Isolation of ATPase I, the proton pump of chromaffin-granule membranes.
Percy, J M, Pryde, J G, Apps, D K
Chromaffin-granule membranes contain two ATPases, which can be separated by (NH4)2SO4 fractionation after solubilization with detergents, or by phase segregation in Triton X-114. ATPase I (Mr 400000)...
Stoichiometry of catecholamine/proton exchange across the chromaffin-granule membrane.
Catecholamines are accumulated by bovine chromaffin-granule "ghosts" in the presence of MgATP at 25 degrees C. With low concentrations of catecholamine, ratios of internal to external amine...
Interaction of pigeon-liver nicotinamide-adenine dinucleotide kinase with cibacron blue F3GA.
The interaction of pigeon liver NAD kinase with Cibacron Blue F3GA was investigated. By using steady-state rate measurements, spectrophotometric titration and chromatography of the enzyme on...
Preparation and properties of nylon-tube-supported nicotinamide--adenine dinucleotide kinase.
Pigeon liver NAD kinase was covalently coupled to the inside surfaces of nylon tubes, both directly to the nylon and via polyamine specers Km values and the inactivation energy of the reaction...
The reversibility of the NAD+ kinase reaction was established, and the kinetic parameters of the rate equation in the reverse direction were determined. The equilibrium constant of the reaction was...
The association of adenosine triphosphatase and ADP/ATP isotope-exchange activities with chromaffin-granule membranes was shown by sucrose-density-gradient centrifugation. The two activities were...
Duncan, R R, Apps, D K, Learmonth, M P, Shipston, M J, Chow, R H
We have examined the expression in bovine adrenal medulla of double C2 protein (DOC2), a vesicular protein which associates with intracellular phospholipid and Ca(2+) and is implicated in the...
Glycosylation and transmembrane topography of bovine chromaffin granule p65.
Tugal, H B, Van Leeuwen, F, Apps, D K, Haywood, J, Phillips, J H
The bovine homologue of p65, a calmodulin-binding protein located in the membranes of synaptic vesicles and endocrine secretory granules, has been studied by the use of monoclonal antibodies directed...
Topography of a vacuolar-type H+-translocating ATPase: chromaffin-granule membrane ATPase I.
Apps, D K, Percy, J M, Perez-Castineira, J R
Proteins exposed on the cytoplasmic face of isolated chromaffin granules were labelled by lactoperoxidase-catalysed radioiodination and by non-enzymic biotinylation. Granule membranes were then...
The proton-translocating adenosine triphosphatase (ATPase) of bovine chromaffin granules contains up to five different polypeptides. Its activity is inhibited by N-ethylmaleimide, and ATP protects...
Perez-Castiñeira, J R, Apps, D K
A procedure has been developed for the rapid purification and reconstitution into phospholipid vesicles of the proton-translocating ATPase of bovine adrenal chromaffin-granule membranes. It involves...
Glycosylation and transmembrane topography of bovine chromaffin granule p65.
Tugal, H B, Van Leeuwen, F, Apps, D K, Haywood, J, Phillips, J H
The bovine homologue of p65, a calmodulin-binding protein located in the membranes of synaptic vesicles and endocrine secretory granules, has been studied by the use of monoclonal antibodies directed...
Isolation of ATPase I, the proton pump of chromaffin-granule membranes.
Percy, J M, Pryde, J G, Apps, D K
Chromaffin-granule membranes contain two ATPases, which can be separated by (NH4)2SO4 fractionation after solubilization with detergents, or by phase segregation in Triton X-114. ATPase I (Mr 400000)...
Stoichiometry of catecholamine/proton exchange across the chromaffin-granule membrane.
Catecholamines are accumulated by bovine chromaffin-granule "ghosts" in the presence of MgATP at 25 degrees C. With low concentrations of catecholamine, ratios of internal to external amine...
Interaction of pigeon-liver nicotinamide-adenine dinucleotide kinase with cibacron blue F3GA.
The interaction of pigeon liver NAD kinase with Cibacron Blue F3GA was investigated. By using steady-state rate measurements, spectrophotometric titration and chromatography of the enzyme on...
Preparation and properties of nylon-tube-supported nicotinamide--adenine dinucleotide kinase.
Pigeon liver NAD kinase was covalently coupled to the inside surfaces of nylon tubes, both directly to the nylon and via polyamine specers Km values and the inactivation energy of the reaction...
The reversibility of the NAD+ kinase reaction was established, and the kinetic parameters of the rate equation in the reverse direction were determined. The equilibrium constant of the reaction was...
The association of adenosine triphosphatase and ADP/ATP isotope-exchange activities with chromaffin-granule membranes was shown by sucrose-density-gradient centrifugation. The two activities were...
Duncan, R R, Apps, D K, Learmonth, M P, Shipston, M J, Chow, R H
We have examined the expression in bovine adrenal medulla of double C2 protein (DOC2), a vesicular protein which associates with intracellular phospholipid and Ca(2+) and is implicated in the...