D. L. Pierson

Publication List Details

Period

2007 - 2007

Number

18

Co-Authors

Evaluation of three portable samplers for monitoring airborne fungi.

Mehta, S K, Mishra, S K, Pierson, D L

Airborne fungi were monitored at five sample sites with the Burkard portable, the RCS Plus, and the SAS Super 90 air samplers; the Andersen 2-stage impactor was used for comparison. All samplers were...

Effect of simulated microgravity and shear stress on microcin B17 production by Escherichia coli and on its excretion into the medium.

Fang, A, Pierson, D L, Koenig, D W, Mishra, S K, Demain, A L

Production of the antibacterial polypeptide microcin B17 (MccB17) by Escherichia coli ZK650 was inhibited by simulated microgravity. The site of MccB17 accumulation was found to be different,...

Gram staining apparatus for space station applications.

Molina, T C, Brown, H D, Irbe, R M, Pierson, D L

A self-contained, portable Gram staining apparatus (GSA) has been developed for use in the microgravity environment on board the Space Station Freedom. Accuracy and reproducibility of this apparatus...

Evaluation of the Biolog automated microbial identification system.

Klingler, J M, Stowe, R P, Obenhuber, D C, Groves, T O, Mishra, S K, Pierson, D L

Biolog's identification system was used to identify 39 American Type Culture Collection reference taxa and 45 gram-negative isolates from water samples. Of the reference strains, 98% were identified...

Development of an antimicrobial susceptibility testing method suitable for performance during space flight.

Jorgensen, J H, Skweres, J A, Mishra, S K, McElmeel, M L, Maher, L A, Mulder, R, ...

Very little is known regarding the effects of the microgravity environment of space flight upon the action of antimicrobial agents on bacterial pathogens. This study was undertaken to develop a...

Isolation and Preparation of Pretyrosine, Accumulated as a Dead-End Metabolite by Neurospora crassa

Jensen, R. A., Zamir, L., St. Pierre, M., Patel, N., Pierson, D. L.

Pretyrosine is an amino acid intermediate of phenylalanine and/or tyrosine biosyntheses in a variety of organisms. A procedure for the isolation of high-quality pretyrosine as the barium salt is...

Stabilization and purification of ornithine transcarbamylase from Neisseria gonorrhoeae.

Powers, C N, Pierson, D L

Ornithine transcarbamylase was stabilized in cell-free extracts by the presence of either carbamyl phosphate or glycerol. The enzyme was rapidly purified by a procedure consisting of ion-exchange...

A549 Lung Epithelial Cells Grown as Three-Dimensional Aggregates: Alternative Tissue Culture Model for Pseudomonas aeruginosa Pathogenesis

Carterson, A. J., Höner Zu Bentrup, K., Ott, C. M., Clarke, M. S., Pierson, D. L., Vanderburg, C. R., ...

A three-dimensional (3-D) lung aggregate model was developed from A549 human lung epithelial cells by using a rotating-wall vessel bioreactor to study the interactions between Pseudomonas aeruginosa...

Evaluation of three portable samplers for monitoring airborne fungi.

Mehta, S K, Mishra, S K, Pierson, D L

Airborne fungi were monitored at five sample sites with the Burkard portable, the RCS Plus, and the SAS Super 90 air samplers; the Andersen 2-stage impactor was used for comparison. All samplers were...

Effect of simulated microgravity and shear stress on microcin B17 production by Escherichia coli and on its excretion into the medium.

Fang, A, Pierson, D L, Koenig, D W, Mishra, S K, Demain, A L

Production of the antibacterial polypeptide microcin B17 (MccB17) by Escherichia coli ZK650 was inhibited by simulated microgravity. The site of MccB17 accumulation was found to be different,...

Gram staining apparatus for space station applications.

Molina, T C, Brown, H D, Irbe, R M, Pierson, D L

A self-contained, portable Gram staining apparatus (GSA) has been developed for use in the microgravity environment on board the Space Station Freedom. Accuracy and reproducibility of this apparatus...

Evaluation of the Biolog automated microbial identification system.

Klingler, J M, Stowe, R P, Obenhuber, D C, Groves, T O, Mishra, S K, Pierson, D L

Biolog's identification system was used to identify 39 American Type Culture Collection reference taxa and 45 gram-negative isolates from water samples. Of the reference strains, 98% were identified...

Development of an antimicrobial susceptibility testing method suitable for performance during space flight.

Jorgensen, J H, Skweres, J A, Mishra, S K, McElmeel, M L, Maher, L A, Mulder, R, ...

Very little is known regarding the effects of the microgravity environment of space flight upon the action of antimicrobial agents on bacterial pathogens. This study was undertaken to develop a...

Isolation and Preparation of Pretyrosine, Accumulated as a Dead-End Metabolite by Neurospora crassa

Jensen, R. A., Zamir, L., St. Pierre, M., Patel, N., Pierson, D. L.

Pretyrosine is an amino acid intermediate of phenylalanine and/or tyrosine biosyntheses in a variety of organisms. A procedure for the isolation of high-quality pretyrosine as the barium salt is...

Stabilization and purification of ornithine transcarbamylase from Neisseria gonorrhoeae.

Powers, C N, Pierson, D L

Ornithine transcarbamylase was stabilized in cell-free extracts by the presence of either carbamyl phosphate or glycerol. The enzyme was rapidly purified by a procedure consisting of ion-exchange...

A549 Lung Epithelial Cells Grown as Three-Dimensional Aggregates: Alternative Tissue Culture Model for Pseudomonas aeruginosa Pathogenesis

Carterson, A. J., Höner Zu Bentrup, K., Ott, C. M., Clarke, M. S., Pierson, D. L., Vanderburg, C. R., ...

A three-dimensional (3-D) lung aggregate model was developed from A549 human lung epithelial cells by using a rotating-wall vessel bioreactor to study the interactions between Pseudomonas aeruginosa...

Space flight alters bacterial gene expression and virulence and reveals a role for global regulator Hfq

Wilson, J. W., Ott, C. M., Zu Bentrup, K. Höner, Ramamurthy, R., Quick, L., Porwollik, S., ...

A comprehensive analysis of both the molecular genetic and phenotypic responses of any organism to the space flight environment has never been accomplished because of significant technological and...