Advection/Diffusion of Large-Scale B-Field in Accretion Disks (2009)
Lovelace, R. V. E., Rothstein, D. M., Bisnovatyi-Kogan, G. S.
Activity of the nuclei of galaxies and stellar mass systems involving disk accretion to black holes is thought to be due to (1) a small-scale turbulent magnetic field in the disk (due to the...
Advection/diffusion of large scale magnetic field in accretion disks (2009)
Activity of the nuclei of galaxies and stellar mass systems involving disk accretion to black holes is thought to be due to (1) a small-scale turbulent magnetic field in the disk (due to the...
Mikles, V. J., Eikenberry, S. S., Rothstein, D. M.
Using observations with the Rossi X-ray Timing Explorer, we examine the behavior of 2-10 Hz quasi-periodic oscillations (QPOs) during spectrally-hard dips in the x-ray light curve of GRS 1915+105...
The Infrared Counterpart of the Microquasar GRS 1758-258 (2002)
Rothstein, D. M., Eikenberry, S. S., Chatterjee, S., Egami, E., Djorgovski, S. G., Heindl, W. A.
We present revised infrared (2.2 micron) astrometry of the field containing the Galactic microquasar GRS 1758-258, using observations at the Keck I 10-m telescope. We find three candidates for the...
Antibody-mediated targeting of CD45 isoforms: A novel immunotherapeutic strategy
Basadonna, G. P., Auersvald, L., Khuong, C. Q., Zheng, X. X., Kashio, N., Zekzer, D., ...
CD45 is a family of transmembrane protein tyrosine phosphatases exclusively expressed by hematopoietic cells and critically involved in the regulation of T cell activation signals. We now demonstrate...
Photoactive Porphyrin Derivative with Broad-Spectrum Activity against Oral Pathogens In Vitro
Rovaldi, C. R., Pievsky, A., Sole, N. A., Friden, P. M., Rothstein, D. M., Spacciapoli, P.
Photodynamic therapy (PDT) has historically been used as a means to treat cancerous tumors but has recently been used to kill bacterial cells through the use of targeted photosensitizers. PDT is a...
Sensitive biological detection method for tetracyclines using a tetA-lacZ fusion system.
Chopra, I, Hacker, K, Misulovin, Z, Rothstein, D M
A sensitive microbiological detection system for tetracyclines, utilizing an Escherichia coli strain containing a cloned tetA-lacZ gene fusion, is described. Expression of beta-galactosidase by the...
McNicholas, P, McGlynn, M, Guay, G G, Rothstein, D M
Genetic analysis of the tetA(C) gene of pBR322 indicates the importance of two-cytoplasmic loops in the TetA(C) protein (P. McNicholas, I. Chopra, and D. M. Rothstein, J. Bacteriol. 174:7926-7933,...
The tetK gene, which encodes a tetracycline efflux pump from Staphylococcus aureus, was expressed in Escherichia coli by using an inducible, low-level expression system. The tetK gene, as well as the...
Detection of tetracyclines and efflux pump inhibitors.
Rothstein, D M, McGlynn, M, Bernan, V, McGahren, J, Zaccardi, J, Cekleniak, N, ...
Screening assays for the detection of tetracyclines and inhibitors of tetracycline efflux pumps are described. The tetracycline assay is based on the observation that the tetA(B) gene encoding the...
Love, S F, Maiese, W M, Rothstein, D M
We report methods to generate protoplasts, to regenerate mycelia, and to transform Micromonospora echinospora. This actinomycete produces the unusual antitumor antibiotics, the calicheamicins. These...
The tet(K) gene from Staphylococcus aureus mediates the transport of potassium in Escherichia coli.
Guay, G G, Tuckman, M, McNicholas, P, Rothstein, D M
The tet(K) gene, encoding the tetracycline efflux protein from Staphylococcus aureus, mediates the transport of potassium in an Escherichia coli mutant defective in potassium uptake. Deletion mapping...
Mutations in the P1 promoter region of Micromonospora echinospora.
We demonstrated previously that the promoters P1a, P1b, and P1c are very closely spaced and are coordinately turned on during stationary phase in Micromonospora echinospora. To determine the...
Genetic analysis of the tetA(C) gene on plasmid pBR322.
McNicholas, P, Chopra, I, Rothstein, D M
The TetA(C) protein, encoded by the tetA(C) gene of plasmid pBR322, is a member of a family of membrane-bound proteins that mediate energy-dependent efflux of tetracycline from the bacterial cell....
Baum, E Z, Buttner, M J, Lin, L S, Rothstein, D M
We demonstrated previously that the 0.4-kilobase DNA fragment from Micromonospora echinospora contains multiple tandem promoters, P1a, P1b, P1c, and P2, which are also functional when cloned into...
Temporally regulated tandem promoters in Micromonospora echinospora.
Baum, E Z, Love, S F, Rothstein, D M
A collection of promoters from the Micromonospora echinospora strain that produces the calichemicin antitumor antibiotics was identified by the use of the promoter-probe vector pIJ486 in Streptomyces...
Isolation of mutants blocked in calicheamicin biosynthesis.
Blocked mutants of Micromonospora echinospora defective in the production of the potent antitumor agent calicheamicin are described. Analysis of intermediates produced by blocked mutants indicates a...
Expression of alpha-amylase in Bacillus licheniformis.
Rothstein, D M, Devlin, P E, Cate, R L
In Bacillus licheniformis, alpha-amylase production varied more than 100-fold depending on the presence or absence of a catabolite-repressing carbon source in the growth medium. alpha-Amylase was...
Reitzer, L J, Bueno, R, Cheng, W D, Abrams, S A, Rothstein, D M, Hunt, T P, ...
Escherichia coli rpoN mutants lack sigma 54 and are therefore unable to initiate the transcription of glnA at glnAp2, which is required for the production of a high intracellular concentration of...
Clostridium thermosaccharolyticum strain deficient in acetate production.
A mutant of Clostridium thermosaccharolyticum that is blocked in acetate production was isolated after treatment with nitrosoguanidine and selection for fluoroacetate resistance. The mutant produced...
Osburne, M S, Craig, R J, Rothstein, D M
We describe a thermoinducible-expression system for Bacillus subtilis which utilized an early promoter-operator sequence from temperate phage phi 105 and the thermolabile prophage repressor from the...
Complex glnA-glnL-glnG operon of Escherichia coli.
Pahel, G, Rothstein, D M, Magasanik, B
The glnG gene product is both a positive regulator and a negative regulator of the expression of glnA, the structural gene for glutamine synthetase, as well as a positive regulator of the expression...
Lipiarmycin-resistant ribonucleic acid polymerase mutants of Bacillus subtilis.
Sonenshein, A L, Alexander, H B, Rothstein, D M, Fisher, S H
Lipiarmycin inhibited the activity of deoxyribonucleic acid-dependent ribonucleic acid (RNA) polymerase in vitro. We showed that inhibition was due to interference by lipiarmycin with the activity of...
Rasmussen, B, Noller, H F, Daubresse, G, Oliva, B, Misulovin, Z, Rothstein, D M, ...
Tetracycline analogs fell into two classes on the basis of their mode of action. Tetracycline, chlortetracycline, minocycline, doxycycline, and 6-demethyl-6-deoxytetracycline inhibited cell-free...
Guay, G G, Tuckman, M, Rothstein, D M
The tetA(B) gene from transposon Tn10 fails to mediate resistance to the novel tetracycline analog 9-(dimethylglycylamido)minocycline (DMG-Mino) (P. E. Sum, V. J. Lee, R. T. Testa, J. J. Hlavka, G....
Isolation of Klebsiella aerogenes mutants cis-dominant for glutamine synthetase expression.
We have isolated three strains of Klebsiella aerogenes that failed to show repression of glutamine synthetase even when grown under the most repressing conditions for the wild-type strain. These...
Rothstein, D M, Pahel, G, Tyler, B, Magasanik, B
One of the suspected regulators of glutamine synthetase [L-glutamate:ammonia ligase (ADP-forming), EC 6.3.1.2] in enteric bacteria is glutamine synthetase itself. We isolated Escherichia coli strains...
Ow, D W, Sundaresan, V, Rothstein, D M, Brown, S E, Ausubel, F M
We have determined the nucleotide sequences of the Klebsiella pneumoniae nifL (regulation of N2 fixation genes) and the Escherichia coli glnA (glutamine synthetase) promoters. We compared these...
Antibody-mediated targeting of CD45 isoforms: A novel immunotherapeutic strategy
Basadonna, G. P., Auersvald, L., Khuong, C. Q., Zheng, X. X., Kashio, N., Zekzer, D., ...
CD45 is a family of transmembrane protein tyrosine phosphatases exclusively expressed by hematopoietic cells and critically involved in the regulation of T cell activation signals. We now demonstrate...
Photoactive Porphyrin Derivative with Broad-Spectrum Activity against Oral Pathogens In Vitro
Rovaldi, C. R., Pievsky, A., Sole, N. A., Friden, P. M., Rothstein, D. M., Spacciapoli, P.
Photodynamic therapy (PDT) has historically been used as a means to treat cancerous tumors but has recently been used to kill bacterial cells through the use of targeted photosensitizers. PDT is a...
Sensitive biological detection method for tetracyclines using a tetA-lacZ fusion system.
Chopra, I, Hacker, K, Misulovin, Z, Rothstein, D M
A sensitive microbiological detection system for tetracyclines, utilizing an Escherichia coli strain containing a cloned tetA-lacZ gene fusion, is described. Expression of beta-galactosidase by the...
McNicholas, P, McGlynn, M, Guay, G G, Rothstein, D M
Genetic analysis of the tetA(C) gene of pBR322 indicates the importance of two-cytoplasmic loops in the TetA(C) protein (P. McNicholas, I. Chopra, and D. M. Rothstein, J. Bacteriol. 174:7926-7933,...
The tetK gene, which encodes a tetracycline efflux pump from Staphylococcus aureus, was expressed in Escherichia coli by using an inducible, low-level expression system. The tetK gene, as well as the...
Detection of tetracyclines and efflux pump inhibitors.
Rothstein, D M, McGlynn, M, Bernan, V, McGahren, J, Zaccardi, J, Cekleniak, N, ...
Screening assays for the detection of tetracyclines and inhibitors of tetracycline efflux pumps are described. The tetracycline assay is based on the observation that the tetA(B) gene encoding the...
Love, S F, Maiese, W M, Rothstein, D M
We report methods to generate protoplasts, to regenerate mycelia, and to transform Micromonospora echinospora. This actinomycete produces the unusual antitumor antibiotics, the calicheamicins. These...
The tet(K) gene from Staphylococcus aureus mediates the transport of potassium in Escherichia coli.
Guay, G G, Tuckman, M, McNicholas, P, Rothstein, D M
The tet(K) gene, encoding the tetracycline efflux protein from Staphylococcus aureus, mediates the transport of potassium in an Escherichia coli mutant defective in potassium uptake. Deletion mapping...
Mutations in the P1 promoter region of Micromonospora echinospora.
We demonstrated previously that the promoters P1a, P1b, and P1c are very closely spaced and are coordinately turned on during stationary phase in Micromonospora echinospora. To determine the...
Genetic analysis of the tetA(C) gene on plasmid pBR322.
McNicholas, P, Chopra, I, Rothstein, D M
The TetA(C) protein, encoded by the tetA(C) gene of plasmid pBR322, is a member of a family of membrane-bound proteins that mediate energy-dependent efflux of tetracycline from the bacterial cell....
Baum, E Z, Buttner, M J, Lin, L S, Rothstein, D M
We demonstrated previously that the 0.4-kilobase DNA fragment from Micromonospora echinospora contains multiple tandem promoters, P1a, P1b, P1c, and P2, which are also functional when cloned into...
Temporally regulated tandem promoters in Micromonospora echinospora.
Baum, E Z, Love, S F, Rothstein, D M
A collection of promoters from the Micromonospora echinospora strain that produces the calichemicin antitumor antibiotics was identified by the use of the promoter-probe vector pIJ486 in Streptomyces...
Isolation of mutants blocked in calicheamicin biosynthesis.
Blocked mutants of Micromonospora echinospora defective in the production of the potent antitumor agent calicheamicin are described. Analysis of intermediates produced by blocked mutants indicates a...
Expression of alpha-amylase in Bacillus licheniformis.
Rothstein, D M, Devlin, P E, Cate, R L
In Bacillus licheniformis, alpha-amylase production varied more than 100-fold depending on the presence or absence of a catabolite-repressing carbon source in the growth medium. alpha-Amylase was...
Reitzer, L J, Bueno, R, Cheng, W D, Abrams, S A, Rothstein, D M, Hunt, T P, ...
Escherichia coli rpoN mutants lack sigma 54 and are therefore unable to initiate the transcription of glnA at glnAp2, which is required for the production of a high intracellular concentration of...
Clostridium thermosaccharolyticum strain deficient in acetate production.
A mutant of Clostridium thermosaccharolyticum that is blocked in acetate production was isolated after treatment with nitrosoguanidine and selection for fluoroacetate resistance. The mutant produced...
Osburne, M S, Craig, R J, Rothstein, D M
We describe a thermoinducible-expression system for Bacillus subtilis which utilized an early promoter-operator sequence from temperate phage phi 105 and the thermolabile prophage repressor from the...
Complex glnA-glnL-glnG operon of Escherichia coli.
Pahel, G, Rothstein, D M, Magasanik, B
The glnG gene product is both a positive regulator and a negative regulator of the expression of glnA, the structural gene for glutamine synthetase, as well as a positive regulator of the expression...
Lipiarmycin-resistant ribonucleic acid polymerase mutants of Bacillus subtilis.
Sonenshein, A L, Alexander, H B, Rothstein, D M, Fisher, S H
Lipiarmycin inhibited the activity of deoxyribonucleic acid-dependent ribonucleic acid (RNA) polymerase in vitro. We showed that inhibition was due to interference by lipiarmycin with the activity of...
Rasmussen, B, Noller, H F, Daubresse, G, Oliva, B, Misulovin, Z, Rothstein, D M, ...
Tetracycline analogs fell into two classes on the basis of their mode of action. Tetracycline, chlortetracycline, minocycline, doxycycline, and 6-demethyl-6-deoxytetracycline inhibited cell-free...
Guay, G G, Tuckman, M, Rothstein, D M
The tetA(B) gene from transposon Tn10 fails to mediate resistance to the novel tetracycline analog 9-(dimethylglycylamido)minocycline (DMG-Mino) (P. E. Sum, V. J. Lee, R. T. Testa, J. J. Hlavka, G....
Isolation of Klebsiella aerogenes mutants cis-dominant for glutamine synthetase expression.
We have isolated three strains of Klebsiella aerogenes that failed to show repression of glutamine synthetase even when grown under the most repressing conditions for the wild-type strain. These...
Rothstein, D M, Pahel, G, Tyler, B, Magasanik, B
One of the suspected regulators of glutamine synthetase [L-glutamate:ammonia ligase (ADP-forming), EC 6.3.1.2] in enteric bacteria is glutamine synthetase itself. We isolated Escherichia coli strains...
Ow, D W, Sundaresan, V, Rothstein, D M, Brown, S E, Ausubel, F M
We have determined the nucleotide sequences of the Klebsiella pneumoniae nifL (regulation of N2 fixation genes) and the Escherichia coli glnA (glutamine synthetase) promoters. We compared these...