Ulrich, F., Concha, M.L., Heid, P.J., Voss, E., Witzel, S., Roehl, H., ...
During vertebrate gastrulation, highly coordinated cellular rearrangements lead to the formation of the three germ layers, ectoderm, mesoderm and endoderm. In zebrafish, silberblick (slb)/wnt11...
Ponticulin plays a role in the positional stabilization of pseudopods (1995)
Shutt, D. C., Wessels, D., Wagenknecht, K., Chandrasekhar, A., Hitt, Anne L., Luna, Elizabeth J., ...
Ponticulin is a 17-kD glycoprotein that represents a major high affinity link between the plasma membrane and the cortical actin network of Dictyostelium. To assess the role of ponticulin in...
Pfaller, M. A., Messer, S. A., Gee, S., Joly, S., Pujol, C., Sullivan, D. J., ...
Candida dubliniensis is a newly recognized fungal pathogen causing mucosal disease in AIDS patients. Although preliminary studies indicate that most strains of C. dubliniensis are susceptible to...
Marco, F., Lockhart, S. R., Pfaller, M. A., Pujol, C., Rangel-Frausto, M. S., Wiblin, T., ...
Computer-assisted DNA fingerprinting with the complex probe Ca3 has been used to analyze the relatedness of isolates collected from individuals with nosocomial bloodstream infections (BSIs) and...
Gales, A. C., Pfaller, M. A., Houston, A. K., Joly, S., Sullivan, D. J., Coleman, D. C., ...
To have a better understanding of the role of Candida dubliniensis in clinical infections, it is essential that microbiology laboratories can identify this species rapidly and accurately in clinical...
Srikantha, T., Tsai, L., Daniels, K., Klar, A. J. S., Soll, D. R.
Five histone deacetylase genes (HDA1, RPD3, HOS1, HOS2, and HOS3) have been cloned from Candida albicans and characterized. Sequence analysis and comparison with 17 additional deacetylases resulted...
Pfaller, M. A., Lockhart, S. R., Pujol, C., Swails-Wenger, J. A., Messer, S. A., Edmond, M. B., ...
In a survey of bloodstream infection (BSI) isolates across the continental United States, 162 Candida albicans isolates were fingerprinted with the species-specific probe Ca3 and the patterns were...
Kvaal, C A, Srikantha, T, Soll, D R
Candida albicans WO-1 switches between a white- and an opaque-colony-forming phenotype. The gene WH11 is expressed differentially in the white phase. The WH11 open reading frame was inserted...
Srikantha, T, Klapach, A, Lorenz, W W, Tsai, L K, Laughlin, L A, Gorman, J A, ...
The infectious yeast Candida albicans progresses through two developmental programs which involve differential gene expression, the bud-hypha transition and high-frequency phenotypic switching. To...
Srikantha, T, Tsai, L K, Soll, D R
Candida albicans strain WO-1 undergoes two developmental programs, the bud-hypha transition and high-frequency phenotypic switching in the form of the white-opaque transition. The WH11 gene is...
Defever, K S, Whelan, W L, Rogers, A L, Beneke, E S, Veselenak, J M, Soll, D R
Resistance to 5-fluorocytosine was studied in 137 independent Candida albicans clinical isolates. Seventy-eight isolates (57%) were susceptible; 51 isolates (37%) were partially resistant; 8 isolates...
Vargas, K, Wertz, P W, Drake, D, Morrow, B, Soll, D R
Cells of the laboratory strain 3153A of Candida albicans can be stimulated to undergo high-frequency phenotypic switching by a low dose of UV. We have compared the adhesive properties of cells...
Telomeric and dispersed repeat sequences in Candida yeasts and their use in strain identification.
Sadhu, C, McEachern, M J, Rustchenko-Bulgac, E P, Schmid, J, Soll, D R, Hicks, J B
Several different repetitive DNA sequences have been isolated from the pathogenic yeast Candida albicans. These include two families of large dispersed repeat sequences (Ca3, Ca24) and a short...
Ultrastructure and antigenicity of the unique cell wall pimple of the Candida opaque phenotype.
Anderson, J, Mihalik, R, Soll, D R
Cells of Candida albicans WO-1 switch frequently and reversibly between two colony-forming phenotypes, white and opaque. In the white form, budding cells appear similar to those of most other strains...
"White-opaque transition": a second high-frequency switching system in Candida albicans.
Slutsky, B, Staebell, M, Anderson, J, Risen, L, Pfaller, M, Soll, D R
A second high-frequency switching system was identified in selected pathogenic strains in the dimorphic yeast Candida albicans. In the characterized strain WO-1, cells switched heritably, reversibly,...
Unique phenotype of opaque cells in the white-opaque transition of Candida albicans.
Select strains of Candida albicans switch reversibly and at extremely high frequency between a white and an opaque colony-forming phenotype, which has been referred to as the white-opaque transition....
Lockhart, S R, Fritch, J J, Meier, A S, Schröppel, K, Srikantha, T, Galask, R, ...
The genetic homogeneity of nine commensal and infecting populations of Candida albicans has been assessed by fingerprinting multiple isolates from each population by Southern blot hybridization first...
Lockhart, S R, Reed, B D, Pierson, C L, Soll, D R
The following three basic scenarios have emerged for the genetic relatedness of strains in recurrent vaginal candidiasis: strain maintenance without genetic variation, strain maintenance with minor...
Frequency, intensity, species, and strains of oral Candida vary as a function of host age.
Kleinegger, C L, Lockhart, S R, Vargas, K, Soll, D R
While the age of the host has been suggested as a determining factor in yeast carriage, no studies in which the genetic relatedness of isolates has been assessed in combination with the frequency and...
Joly, S, Pujol, C, Schröppel, K, Soll, D R
Candida tropicalis has emerged as the second most frequent colonizing Candida species, and it has been documented in nosocomial infections. To develop an effective fingerprinting system for this...
Pujol, C, Joly, S, Lockhart, S R, Noel, S, Tibayrenc, M, Soll, D R
Randomly amplified polymorphic DNA (RAPD) analysis, multilocus enzyme electrophoresis (MLEE), and Southern blot hybridization with moderately repetitive DNA probes have emerged as effective...
Functional analysis of the promoter of the phase-specific WH11 gene of Candida albicans.
Srikantha, T, Chandrasekhar, A, Soll, D R
Candida albicans WO-1 switches spontaneously, frequently, and reversibly between a hemispherical white and a flat gray (opaque) colony-forming phenotype. This transition affects a number of...
When cultures of Candida albicans which had entered stationary phase due to the depletion of zinc (zinc-limiting conditions) were compared with cultures which had entered stationary phase due to the...
Genetic similarity of Candida albicans strains from vaginitis patients and their partners.
Schmid, J, Rotman, M, Reed, B, Pierson, C L, Soll, D R
The moderately repetitive sequence Ca3 was used to fingerprint strains of Candida albicans isolated from vulvovaginal infections of 10 women and strains isolated from their male partners. The Dendron...
Schröppel, K, Rotman, M, Galask, R, Mac, K, Soll, D R
Southern blot hybridization with the Ca3 probe and the C fragment of the Ca3 probe was used to assess the genetic relatedness of Candida albicans strains from one patient with recurrent C. albicans...
Schmid, J, Odds, F C, Wiselka, M J, Nicholson, K G, Soll, D R
By using the computer-assisted Dendron system to analyze the patterns of Southern blots probed with the repetitive sequence Ca3, we have compared oral isolates of Candida spp. from a group of 11...
Anderson, J, Srikantha, T, Morrow, B, Miyasaki, S H, White, T C, Agabian, N, ...
The moderately repetitive Ca3 fragment of Candida albicans has been used as an effective DNA fingerprinting probe in epidemiological studies. EcoRI digestion of Ca3 DNA results in seven fragments of...
Development of DNA probes for fingerprinting Aspergillus fumigatus.
Girardin, H, Latgé, J P, Srikantha, T, Morrow, B, Soll, D R
Several different DNA fragments containing nonribosomal repetitive sequences have been isolated from the genome of Aspergillus fumigatus and tested as potential DNA fingerprinting probes. Eight of...
Hellstein, J, Vawter-Hugart, H, Fotos, P, Schmid, J, Soll, D R
Colony phenotype and genetic similarity were assessed within and between groups of commensal and pathogenic strains of Candida albicans collected from the oral cavities of individuals in a single...
Multiple Candida strains in the course of a single systemic infection.
Soll, D R, Staebell, M, Langtimm, C, Pfaller, M, Hicks, J, Rao, T V
Species and strain variabilities have been monitored during the history of a prolonged Candida infection in a single compromised bone marrow transplant patient by analyzing sugar assimilation...
Switching of Candida albicans during successive episodes of recurrent vaginitis.
Soll, D R, Galask, R, Isley, S, Rao, T V, Stone, D, Hicks, J, ...
Strain relatedness and switching were monitored in Candida albicans strains isolated from different body locations through three episodes of recurrent vulvovaginal candidiasis separated by two...
When used to probe EcoRI-digested Candida albicans DNA, the moderately repetitive sequence Ca3 generated a Southern blot hybridization pattern which included 15 to 25 bands, depending upon the...
High-frequency switching in Candida strains isolated from vaginitis patients.
Soll, D R, Langtimm, C J, McDowell, J, Hicks, J, Galask, R
High-frequency switching and strain variability at the site of infection was assessed in 11 patients with acute Candida albicans vaginitis. By cloning cells directly from the site of infection, it...
Soll, D R, Galask, R, Schmid, J, Hanna, C, Mac, K, Morrow, B
Candida spp. carriage and strain relatedness were assessed in 52 healthy women at 17 anatomical locations by using an isolation procedure which assesses carriage intensity and by using a...
Van Soolingen, D, Hermans, P W, De Haas, P E, Soll, D R, Van Embden, J D
In this study we established the usefulness of DNA fingerprinting for the epidemiology of tuberculosis on the basis of the DNA polymorphism generated by the insertion sequence (IS) IS986. Although...
Cox, D, Wessels, D, Soll, D R, Hartwig, J, Condeelis, J
The actin binding protein ABP-120 has been proposed to cross-link actin filaments in nascent pseudopods, in a step required for normal pseudopod extension in motile Dictyostelium amoebae. To test...
Morrow, B, Srikantha, T, Anderson, J, Soll, D R
Cells of Candida albicans WO-1 switch spontaneously and frequently between a white and an opaque CFU. Recently, an opaque-phase-specific cDNA, PEP1, was cloned and was demonstrated to code for a...
Chemotaxis to cAMP and slug migration in Dictyostelium both depend on migA, a BTB protein.
Escalante, R, Wessels, D, Soll, D R, Loomis, W F
Chemotaxis in natural aggregation territories and in a chamber with an imposed gradient of cyclic AMP (cAMP) was found to be defective in a mutant strain of Dictyostelium discoideum that forms slugs...
Hypha formation in the white-opaque transition of Candida albicans.
Anderson, J, Cundiff, L, Schnars, B, Gao, M X, Mackenzie, I, Soll, D R
Cells of Candida albicans strain WO-1 and related strains switch frequently and reversibly between a white-colony-forming unit (white phase) and a gray-colony-forming unit (opaque phase). Cells in...
Zinc and regulation of growth and phenotype in the infectious yeast Candida albicans.
Soll, D R, Bedell, G W, Brummel, M
When Candida albicans is grown at 25 degrees C in suspension in defined medium, cells accumulate at stationary phase as singlets in G1 of the deoxyribonucleic acid replication cycle and acquire the...
High-frequency switching in Candida albicans.
Most strains of Candida albicans are capable of switching frequently and reversibly between a number of phenotypes distinguishable by colony morphology. A number of different switching systems have...
Morrow, B, Srikantha, T, Soll, D R
Cells of Candida albicans WO-1 spontaneously switch between a white and opaque CFU, and this phase transition involves a dramatic change in cellular phenotype. By using a differential hybridization...
Zoospore Germination in Blastocladiella emersonii: Cell Differentiation without Protein Synthesis?*
Evidence is presented to suggest that several early events of zoospore germination in the water mold, Blastocladiella emersonii, are not dependent upon concomitant protein synthesis. The events...
In this analysis we have examined in detail the effects of low concentrations of zinc on the growth and dimorphism of Candida albicans. Evidence is presented that micromolar concentrations of zinc...
Klar, A J, Srikantha, T, Soll, D R
Most strains of Candida albicans undergo high frequency phenotypic switching. Strain WO-1 undergoes the white-opaque transition, which involves changes in colony and cellular morphology, gene...
Pfaller, M. A., Messer, S. A., Gee, S., Joly, S., Pujol, C., Sullivan, D. J., ...
Candida dubliniensis is a newly recognized fungal pathogen causing mucosal disease in AIDS patients. Although preliminary studies indicate that most strains of C. dubliniensis are susceptible to...
Marco, F., Lockhart, S. R., Pfaller, M. A., Pujol, C., Rangel-Frausto, M. S., Wiblin, T., ...
Computer-assisted DNA fingerprinting with the complex probe Ca3 has been used to analyze the relatedness of isolates collected from individuals with nosocomial bloodstream infections (BSIs) and...
Gales, A. C., Pfaller, M. A., Houston, A. K., Joly, S., Sullivan, D. J., Coleman, D. C., ...
To have a better understanding of the role of Candida dubliniensis in clinical infections, it is essential that microbiology laboratories can identify this species rapidly and accurately in clinical...
Srikantha, T., Tsai, L., Daniels, K., Klar, A. J. S., Soll, D. R.
Five histone deacetylase genes (HDA1, RPD3, HOS1, HOS2, and HOS3) have been cloned from Candida albicans and characterized. Sequence analysis and comparison with 17 additional deacetylases resulted...
Pfaller, M. A., Lockhart, S. R., Pujol, C., Swails-Wenger, J. A., Messer, S. A., Edmond, M. B., ...
In a survey of bloodstream infection (BSI) isolates across the continental United States, 162 Candida albicans isolates were fingerprinted with the species-specific probe Ca3 and the patterns were...
Kvaal, C A, Srikantha, T, Soll, D R
Candida albicans WO-1 switches between a white- and an opaque-colony-forming phenotype. The gene WH11 is expressed differentially in the white phase. The WH11 open reading frame was inserted...
Srikantha, T, Klapach, A, Lorenz, W W, Tsai, L K, Laughlin, L A, Gorman, J A, ...
The infectious yeast Candida albicans progresses through two developmental programs which involve differential gene expression, the bud-hypha transition and high-frequency phenotypic switching. To...
Srikantha, T, Tsai, L K, Soll, D R
Candida albicans strain WO-1 undergoes two developmental programs, the bud-hypha transition and high-frequency phenotypic switching in the form of the white-opaque transition. The WH11 gene is...
Defever, K S, Whelan, W L, Rogers, A L, Beneke, E S, Veselenak, J M, Soll, D R
Resistance to 5-fluorocytosine was studied in 137 independent Candida albicans clinical isolates. Seventy-eight isolates (57%) were susceptible; 51 isolates (37%) were partially resistant; 8 isolates...
Vargas, K, Wertz, P W, Drake, D, Morrow, B, Soll, D R
Cells of the laboratory strain 3153A of Candida albicans can be stimulated to undergo high-frequency phenotypic switching by a low dose of UV. We have compared the adhesive properties of cells...
Telomeric and dispersed repeat sequences in Candida yeasts and their use in strain identification.
Sadhu, C, McEachern, M J, Rustchenko-Bulgac, E P, Schmid, J, Soll, D R, Hicks, J B
Several different repetitive DNA sequences have been isolated from the pathogenic yeast Candida albicans. These include two families of large dispersed repeat sequences (Ca3, Ca24) and a short...
Ultrastructure and antigenicity of the unique cell wall pimple of the Candida opaque phenotype.
Anderson, J, Mihalik, R, Soll, D R
Cells of Candida albicans WO-1 switch frequently and reversibly between two colony-forming phenotypes, white and opaque. In the white form, budding cells appear similar to those of most other strains...
"White-opaque transition": a second high-frequency switching system in Candida albicans.
Slutsky, B, Staebell, M, Anderson, J, Risen, L, Pfaller, M, Soll, D R
A second high-frequency switching system was identified in selected pathogenic strains in the dimorphic yeast Candida albicans. In the characterized strain WO-1, cells switched heritably, reversibly,...
Unique phenotype of opaque cells in the white-opaque transition of Candida albicans.
Select strains of Candida albicans switch reversibly and at extremely high frequency between a white and an opaque colony-forming phenotype, which has been referred to as the white-opaque transition....
Lockhart, S R, Fritch, J J, Meier, A S, Schröppel, K, Srikantha, T, Galask, R, ...
The genetic homogeneity of nine commensal and infecting populations of Candida albicans has been assessed by fingerprinting multiple isolates from each population by Southern blot hybridization first...
Lockhart, S R, Reed, B D, Pierson, C L, Soll, D R
The following three basic scenarios have emerged for the genetic relatedness of strains in recurrent vaginal candidiasis: strain maintenance without genetic variation, strain maintenance with minor...
Frequency, intensity, species, and strains of oral Candida vary as a function of host age.
Kleinegger, C L, Lockhart, S R, Vargas, K, Soll, D R
While the age of the host has been suggested as a determining factor in yeast carriage, no studies in which the genetic relatedness of isolates has been assessed in combination with the frequency and...
Joly, S, Pujol, C, Schröppel, K, Soll, D R
Candida tropicalis has emerged as the second most frequent colonizing Candida species, and it has been documented in nosocomial infections. To develop an effective fingerprinting system for this...
Pujol, C, Joly, S, Lockhart, S R, Noel, S, Tibayrenc, M, Soll, D R
Randomly amplified polymorphic DNA (RAPD) analysis, multilocus enzyme electrophoresis (MLEE), and Southern blot hybridization with moderately repetitive DNA probes have emerged as effective...
Functional analysis of the promoter of the phase-specific WH11 gene of Candida albicans.
Srikantha, T, Chandrasekhar, A, Soll, D R
Candida albicans WO-1 switches spontaneously, frequently, and reversibly between a hemispherical white and a flat gray (opaque) colony-forming phenotype. This transition affects a number of...
When cultures of Candida albicans which had entered stationary phase due to the depletion of zinc (zinc-limiting conditions) were compared with cultures which had entered stationary phase due to the...
Genetic similarity of Candida albicans strains from vaginitis patients and their partners.
Schmid, J, Rotman, M, Reed, B, Pierson, C L, Soll, D R
The moderately repetitive sequence Ca3 was used to fingerprint strains of Candida albicans isolated from vulvovaginal infections of 10 women and strains isolated from their male partners. The Dendron...
Schröppel, K, Rotman, M, Galask, R, Mac, K, Soll, D R
Southern blot hybridization with the Ca3 probe and the C fragment of the Ca3 probe was used to assess the genetic relatedness of Candida albicans strains from one patient with recurrent C. albicans...
Schmid, J, Odds, F C, Wiselka, M J, Nicholson, K G, Soll, D R
By using the computer-assisted Dendron system to analyze the patterns of Southern blots probed with the repetitive sequence Ca3, we have compared oral isolates of Candida spp. from a group of 11...
Anderson, J, Srikantha, T, Morrow, B, Miyasaki, S H, White, T C, Agabian, N, ...
The moderately repetitive Ca3 fragment of Candida albicans has been used as an effective DNA fingerprinting probe in epidemiological studies. EcoRI digestion of Ca3 DNA results in seven fragments of...
Development of DNA probes for fingerprinting Aspergillus fumigatus.
Girardin, H, Latgé, J P, Srikantha, T, Morrow, B, Soll, D R
Several different DNA fragments containing nonribosomal repetitive sequences have been isolated from the genome of Aspergillus fumigatus and tested as potential DNA fingerprinting probes. Eight of...
Hellstein, J, Vawter-Hugart, H, Fotos, P, Schmid, J, Soll, D R
Colony phenotype and genetic similarity were assessed within and between groups of commensal and pathogenic strains of Candida albicans collected from the oral cavities of individuals in a single...
Multiple Candida strains in the course of a single systemic infection.
Soll, D R, Staebell, M, Langtimm, C, Pfaller, M, Hicks, J, Rao, T V
Species and strain variabilities have been monitored during the history of a prolonged Candida infection in a single compromised bone marrow transplant patient by analyzing sugar assimilation...
Switching of Candida albicans during successive episodes of recurrent vaginitis.
Soll, D R, Galask, R, Isley, S, Rao, T V, Stone, D, Hicks, J, ...
Strain relatedness and switching were monitored in Candida albicans strains isolated from different body locations through three episodes of recurrent vulvovaginal candidiasis separated by two...
When used to probe EcoRI-digested Candida albicans DNA, the moderately repetitive sequence Ca3 generated a Southern blot hybridization pattern which included 15 to 25 bands, depending upon the...
High-frequency switching in Candida strains isolated from vaginitis patients.
Soll, D R, Langtimm, C J, McDowell, J, Hicks, J, Galask, R
High-frequency switching and strain variability at the site of infection was assessed in 11 patients with acute Candida albicans vaginitis. By cloning cells directly from the site of infection, it...
Soll, D R, Galask, R, Schmid, J, Hanna, C, Mac, K, Morrow, B
Candida spp. carriage and strain relatedness were assessed in 52 healthy women at 17 anatomical locations by using an isolation procedure which assesses carriage intensity and by using a...
Van Soolingen, D, Hermans, P W, De Haas, P E, Soll, D R, Van Embden, J D
In this study we established the usefulness of DNA fingerprinting for the epidemiology of tuberculosis on the basis of the DNA polymorphism generated by the insertion sequence (IS) IS986. Although...
Cox, D, Wessels, D, Soll, D R, Hartwig, J, Condeelis, J
The actin binding protein ABP-120 has been proposed to cross-link actin filaments in nascent pseudopods, in a step required for normal pseudopod extension in motile Dictyostelium amoebae. To test...
Morrow, B, Srikantha, T, Anderson, J, Soll, D R
Cells of Candida albicans WO-1 switch spontaneously and frequently between a white and an opaque CFU. Recently, an opaque-phase-specific cDNA, PEP1, was cloned and was demonstrated to code for a...
Chemotaxis to cAMP and slug migration in Dictyostelium both depend on migA, a BTB protein.
Escalante, R, Wessels, D, Soll, D R, Loomis, W F
Chemotaxis in natural aggregation territories and in a chamber with an imposed gradient of cyclic AMP (cAMP) was found to be defective in a mutant strain of Dictyostelium discoideum that forms slugs...
Hypha formation in the white-opaque transition of Candida albicans.
Anderson, J, Cundiff, L, Schnars, B, Gao, M X, Mackenzie, I, Soll, D R
Cells of Candida albicans strain WO-1 and related strains switch frequently and reversibly between a white-colony-forming unit (white phase) and a gray-colony-forming unit (opaque phase). Cells in...
Zinc and regulation of growth and phenotype in the infectious yeast Candida albicans.
Soll, D R, Bedell, G W, Brummel, M
When Candida albicans is grown at 25 degrees C in suspension in defined medium, cells accumulate at stationary phase as singlets in G1 of the deoxyribonucleic acid replication cycle and acquire the...
High-frequency switching in Candida albicans.
Most strains of Candida albicans are capable of switching frequently and reversibly between a number of phenotypes distinguishable by colony morphology. A number of different switching systems have...
Morrow, B, Srikantha, T, Soll, D R
Cells of Candida albicans WO-1 spontaneously switch between a white and opaque CFU, and this phase transition involves a dramatic change in cellular phenotype. By using a differential hybridization...
Zoospore Germination in Blastocladiella emersonii: Cell Differentiation without Protein Synthesis?*
Evidence is presented to suggest that several early events of zoospore germination in the water mold, Blastocladiella emersonii, are not dependent upon concomitant protein synthesis. The events...
In this analysis we have examined in detail the effects of low concentrations of zinc on the growth and dimorphism of Candida albicans. Evidence is presented that micromolar concentrations of zinc...
Klar, A J, Srikantha, T, Soll, D R
Most strains of Candida albicans undergo high frequency phenotypic switching. Strain WO-1 undergoes the white-opaque transition, which involves changes in colony and cellular morphology, gene...