Structure of Sendai viral proteins in plasma membranes of virus-infected cells.
Purified plasma membranes attached to polycationic polyacrylamide beads by their external surface were isolated from BHK cells infected with Sendai virus. Each of the viral proteins could be...
Subcellular localization of the env-related glycoproteins in Friend erythroleukemia cells.
A scheme was developed for the subcellular fractionation of murine erythroleukemia cells transformed by Friend leukemia virus. The subcellular localization of the env-related glycoproteins was...
Effect of vesicular stomatitis virus matrix protein on host-directed translation in vivo.
Black, B L, Brewer, G, Lyles, D S
Vesicular stomatitis virus infection causes a rapid and potent inhibition of both host transcription and translation. Recently, the viral matrix (M) protein was shown to inhibit host-directed...
Taylor, K A, Wetzel, S, Lyles, D S, Pollok, B A
The use of different viral promoters for the expression of the EBNA1 gene product appears to be a critical step in the regulation of Epstein-Barr virus latent gene expression and may reflect the...
Black, B L, Rhodes, R B, McKenzie, M, Lyles, D S
Recently, the vesicular stomatitis virus matrix (M) protein has been shown to be capable of inhibition of host cell-directed transcription in the absence of other viral components (B. L. Black and D....
Lyles, D S, McKenzie, M, Parce, J W
The mechanism by which viral glycoproteins are incorporated into virus envelopes during budding from host membranes is a major question of virus assembly. Evidence is presented here that the envelope...
Sequences of the vesicular stomatitis virus matrix protein involved in binding to nucleocapsids.
Kaptur, P E, Rhodes, R B, Lyles, D S
The purpose of these experiments was to study the physical structure of the nucleocapsid-M protein complex of vesicular stomatitis virus by analysis of nucleocapsid binding by wild-type and mutant M...
Infection by vesicular stomatitis virus (VSV) results in a rapid inhibition of host cell transcription and translation. To determine whether the viral matrix (M) protein was involved in this...
McCreedy, B J, McKinnon, K P, Lyles, D S
The peripheral membrane M protein of vesicular stomatitis virus purified by detergent extraction of virions and ion-exchange chromatography was determined to be a monomer in the absence of detergent...
Roscoe, D M, Ishikawa, K, Lyles, D S
The requirements for viral and host protein synthesis in the generation of target antigens for cytotoxic T lymphocytes (CTL) was evaluated by using vesicular stomatitis virus (VSV) inactivated by UV...
The interaction of Sendai viral proteins with the membranes of infected cells during budding of progeny virions was studied. BHK cells infected with Sendai virus were labeled with [35S]methionine,...
Vesicular stomatitis virus M protein in the nuclei of infected cells.
Lyles, D S, Puddington, L, McCreedy, B J
The M protein of vesicular stomatitis virus (VSV) was localized in the nuclei and cytoplasm of VSV-infected cells by subcellular fractionation and immunofluorescence microscopy. Nuclei isolated from...
Bowman, M R, Lyles, D S, Parce, J W
Spleen cells from C57BL/6 (B6) mice generate a strong in vitro cytotoxic T-lymphocyte (CTL) response specific for vesicular stomatitis virus (VSV). Spleen cells from VSV-primed B6-H-2bm3 (bm3) mice,...
Greenspan, D, Krystal, M, Nakada, S, Arnheiter, H, Lyles, D S, Palese, P
The nonstructural NS2 protein of influenza A/PR/8/34 virus was efficiently expressed in bacteria, and monospecific antisera were prepared against the bacterially synthesized polypeptide. These...
Synthesis of viral proteins in polymorphonuclear leukocytes infected with influenza A virus.
Cassidy, L F, Lyles, D S, Abramson, J S
Various reports have indicated that infection of polymorphonuclear leukocytes (PMNL) with influenza virus causes depression of their metabolic and chemotactic responses, but the effect the PMNL has...
Neuraminidase activity is not the cause of influenza virus-induced neutrophil dysfunction.
Abramson, J S, Wiegand, G L, Lyles, D S
Influenza viruses have been shown to decrease the ability of polymorphonuclear leukocytes (PMN) to respond to a variety of stimuli. This study was done to determine if viral neuraminidase was...
Sites of in vivo phosphorylation of vesicular stomatitis virus matrix protein.
Kaptur, P E, McCreedy, B J, Lyles, D S
We mapped the in vivo phosphorylation sites for the matrix (M) protein of the Orsay and San Juan strains of vesicular stomatitis virus, Indiana serotype, using limited proteolysis and phosphoamino...
Influenza A virus-induced polymorphonuclear leukocyte dysfunction.
Abramson, J S, Lyles, D S, Heller, K A, Bass, D A
Previous studies have shown that influenza A virus can activate the polymorphonuclear leukocyte (PMN) respiratory burst and that upon subsequent stimulation of the cell there is depressed metabolic...
Hale, A H, Ruebush, M J, Lyles, D S, Harris, D T
A method of liposome modification of cell surfaces to render unsuitable target cells susceptible to lysis by anti-viral cytotoxic T lymphocytes (CTLs) is described. Liposomes containing the...
Abramson, J S, Lewis, J C, Lyles, D S, Heller, K A, Mills, E L, Bass, D A
The present study examined the effect of various unopsonized strains of influenza A virus on release of myeloperoxidase (MPO) and acid phosphatase in polymorphonuclear leukocytes (PMNL). These...
Glycoproteins of Sendai virus are transmembrane proteins.
Radiolabeled Sendai viral envelope proteins were incorporated into human erythrocyte membranes by the process of fusion of the viral envelope with the erythrocyte membrane. Inside-out (IO) vesicles...
Sendai virus-induced hemolysis: reduction in heterogeneity of erythrocyte lipid bilayer fluidity.
Hemolysis of human or chicken erythrocytes by Sendai virus causes a change in the structure of the erythrocyte membrane lipid bilayer that can be detected by spin label electron spin resonance. In...
Techniques of spin-label electron spin resonance have been used to prove changes in the structure of the lipid phase of erythrocyte membrane after agglutination by viruses and lectins. When chicken...
Structure of Sendai viral proteins in plasma membranes of virus-infected cells.
Purified plasma membranes attached to polycationic polyacrylamide beads by their external surface were isolated from BHK cells infected with Sendai virus. Each of the viral proteins could be...
Subcellular localization of the env-related glycoproteins in Friend erythroleukemia cells.
A scheme was developed for the subcellular fractionation of murine erythroleukemia cells transformed by Friend leukemia virus. The subcellular localization of the env-related glycoproteins was...
Effect of vesicular stomatitis virus matrix protein on host-directed translation in vivo.
Black, B L, Brewer, G, Lyles, D S
Vesicular stomatitis virus infection causes a rapid and potent inhibition of both host transcription and translation. Recently, the viral matrix (M) protein was shown to inhibit host-directed...
Taylor, K A, Wetzel, S, Lyles, D S, Pollok, B A
The use of different viral promoters for the expression of the EBNA1 gene product appears to be a critical step in the regulation of Epstein-Barr virus latent gene expression and may reflect the...
Black, B L, Rhodes, R B, McKenzie, M, Lyles, D S
Recently, the vesicular stomatitis virus matrix (M) protein has been shown to be capable of inhibition of host cell-directed transcription in the absence of other viral components (B. L. Black and D....
Lyles, D S, McKenzie, M, Parce, J W
The mechanism by which viral glycoproteins are incorporated into virus envelopes during budding from host membranes is a major question of virus assembly. Evidence is presented here that the envelope...
Sequences of the vesicular stomatitis virus matrix protein involved in binding to nucleocapsids.
Kaptur, P E, Rhodes, R B, Lyles, D S
The purpose of these experiments was to study the physical structure of the nucleocapsid-M protein complex of vesicular stomatitis virus by analysis of nucleocapsid binding by wild-type and mutant M...
Infection by vesicular stomatitis virus (VSV) results in a rapid inhibition of host cell transcription and translation. To determine whether the viral matrix (M) protein was involved in this...
McCreedy, B J, McKinnon, K P, Lyles, D S
The peripheral membrane M protein of vesicular stomatitis virus purified by detergent extraction of virions and ion-exchange chromatography was determined to be a monomer in the absence of detergent...
Roscoe, D M, Ishikawa, K, Lyles, D S
The requirements for viral and host protein synthesis in the generation of target antigens for cytotoxic T lymphocytes (CTL) was evaluated by using vesicular stomatitis virus (VSV) inactivated by UV...
The interaction of Sendai viral proteins with the membranes of infected cells during budding of progeny virions was studied. BHK cells infected with Sendai virus were labeled with [35S]methionine,...
Vesicular stomatitis virus M protein in the nuclei of infected cells.
Lyles, D S, Puddington, L, McCreedy, B J
The M protein of vesicular stomatitis virus (VSV) was localized in the nuclei and cytoplasm of VSV-infected cells by subcellular fractionation and immunofluorescence microscopy. Nuclei isolated from...
Bowman, M R, Lyles, D S, Parce, J W
Spleen cells from C57BL/6 (B6) mice generate a strong in vitro cytotoxic T-lymphocyte (CTL) response specific for vesicular stomatitis virus (VSV). Spleen cells from VSV-primed B6-H-2bm3 (bm3) mice,...
Greenspan, D, Krystal, M, Nakada, S, Arnheiter, H, Lyles, D S, Palese, P
The nonstructural NS2 protein of influenza A/PR/8/34 virus was efficiently expressed in bacteria, and monospecific antisera were prepared against the bacterially synthesized polypeptide. These...
Synthesis of viral proteins in polymorphonuclear leukocytes infected with influenza A virus.
Cassidy, L F, Lyles, D S, Abramson, J S
Various reports have indicated that infection of polymorphonuclear leukocytes (PMNL) with influenza virus causes depression of their metabolic and chemotactic responses, but the effect the PMNL has...
Neuraminidase activity is not the cause of influenza virus-induced neutrophil dysfunction.
Abramson, J S, Wiegand, G L, Lyles, D S
Influenza viruses have been shown to decrease the ability of polymorphonuclear leukocytes (PMN) to respond to a variety of stimuli. This study was done to determine if viral neuraminidase was...
Sites of in vivo phosphorylation of vesicular stomatitis virus matrix protein.
Kaptur, P E, McCreedy, B J, Lyles, D S
We mapped the in vivo phosphorylation sites for the matrix (M) protein of the Orsay and San Juan strains of vesicular stomatitis virus, Indiana serotype, using limited proteolysis and phosphoamino...
Influenza A virus-induced polymorphonuclear leukocyte dysfunction.
Abramson, J S, Lyles, D S, Heller, K A, Bass, D A
Previous studies have shown that influenza A virus can activate the polymorphonuclear leukocyte (PMN) respiratory burst and that upon subsequent stimulation of the cell there is depressed metabolic...
Hale, A H, Ruebush, M J, Lyles, D S, Harris, D T
A method of liposome modification of cell surfaces to render unsuitable target cells susceptible to lysis by anti-viral cytotoxic T lymphocytes (CTLs) is described. Liposomes containing the...
Abramson, J S, Lewis, J C, Lyles, D S, Heller, K A, Mills, E L, Bass, D A
The present study examined the effect of various unopsonized strains of influenza A virus on release of myeloperoxidase (MPO) and acid phosphatase in polymorphonuclear leukocytes (PMNL). These...
Glycoproteins of Sendai virus are transmembrane proteins.
Radiolabeled Sendai viral envelope proteins were incorporated into human erythrocyte membranes by the process of fusion of the viral envelope with the erythrocyte membrane. Inside-out (IO) vesicles...
Sendai virus-induced hemolysis: reduction in heterogeneity of erythrocyte lipid bilayer fluidity.
Hemolysis of human or chicken erythrocytes by Sendai virus causes a change in the structure of the erythrocyte membrane lipid bilayer that can be detected by spin label electron spin resonance. In...
Techniques of spin-label electron spin resonance have been used to prove changes in the structure of the lipid phase of erythrocyte membrane after agglutination by viruses and lectins. When chicken...