Helix packing of lactose permease in Escherichia coli studied by site-directed chemical cleavage.
Wu, J, Perrin, D M, Sigman, D S, Kaback, H R
Biotinylated lactose permease from Escherichia coli containing a single-cysteine residue at position 330 (helix X) or at position 147, 148, or 149 (helix V) was purified by avidin-affinity...
Chen, C B, Gorin, M B, Sigman, D S
RNAs modified with the chemical nuclease 1,10-phenanthroline-copper(I) can achieve the sequence-specific scission of single- and double-stranded DNA targets. The RNAs are prepared in vitro by using...
A transcription inhibitor specific for unwound DNA in RNA polymerase-promoter open complexes.
Mazumder, A, Perrin, D M, Watson, K J, Sigman, D S
The kinetically component open complexes formed at prokaryotic and eukaryotic transcription start sites are efficiently nicked by the chemical nuclease activity of the 2:1...
Li, C, Lai, C F, Sigman, D S, Gaynor, R B
Human immunodeficiency virus (HIV) gene expression is regulated by both general transcription factors and factors induced by activation of T lymphocytes such as NF-kappa B and the nuclear factor of...
Kinetics of spontaneous displacement of RNA from heteroduplexes by DNA.
Landgraf, R, Ramamurthi, K S, Sigman, D S
We have used R-loop formation and direct hybridization techniques to analyze the kinetics by which RNA is displaced from a heteroduplex by DNA of identical sequence. Using random walk simulations we...
Gaynor, R B, Kuwabara, M D, Wu, F K, Garcia, J A, Harrich, D, Briskin, M, ...
The enhancer element of the human immunodeficiency virus type I (HIV-I) long terminal repeat (LTR) contains two copies of nearly identical sequences AGGGACTTTCC (3G sequence) and GGGGACTTTCC (4G...
Gaynor, R, Soultanakis, E, Kuwabara, M, Garcia, J, Sigman, D S
The transactivator protein, tat, encoded by the human immunodeficiency virus is a key regulator of viral transcription. Activation by the tat protein requires sequences downstream of the...
Law, R, Kuwabara, M D, Briskin, M, Fasel, N, Hermanson, G, Sigman, D S, ...
mRNAs specifying immunoglobulin mu and delta heavy chains are encoded by a single large, complex transcription unit (mu + delta gene). The transcriptional activity of delta gene segments in...
R-loop stability as a function of RNA structure and size.
Landgraf, R, Chen, C H, Sigman, D S
The sequence-specific formation of R-loops can be assayed using RNAs which overlap a HindIII cleavage site in a 3.5 kb plasmid. Chemical modification of the displaced DNA strand has permitted...
Double stranded scission of DNA directed through sequence-specific R-loop formation.
Landgraf, R, Chen, C H, Sigman, D S
R-loop formation with short (100 nt) RNAs provides a highly flexible and stringent method to achieve sequence-specific separation of target DNA at any given sequence. After stabilization of R-loops...
Murakawa, G J, Chen, C H, Kuwabara, M D, Nierlich, D P, Sigman, D S
The scission of RNA by the chemical nuclease activity of 1,10-phenanthroline-copper (OP-Cu) has been studied using a lac mRNA fragment and tRNAphe as substrates. Since the chemical mechanism of...
Young, A. P., Brown, F. F., Halsey, M. J., Sigman, D. S.
Incubation of membrane fragments bearing acetylcholine receptors from Torpedo californica under an atmosphere of 3% halothane, 1% chloroform, or 6% diethyl ether greatly facilitates the...
Secondary structure specificity of the nuclease activity of the 1,10-phenanthroline-copper complex.
The artificial DNase activity of the 1,10-phenanthroline-cuprous ion complex [(OP)2Cu+] and H2O2 cleaves the A, B, and Z forms of DNA at different rates. The B structure, formed by most DNAs...
Nuclease activity of 1,10-phenanthroline-copper: sequence-specific targeting.
The nuclease activity of 1,10-phenanthroline-copper ion can be targeted to specific DNA sequences by attachment of the ligand to the 5' end of complementary deoxyoligonucleotides via a...
Pearson, L, Chen, C B, Gaynor, R P, Sigman, D S
RNA-protein complexes isolated following a gel retardation assay can be footprinted within the gel matrix using the chemical nuclease activities of 4,7-dimethyl-, 5,6-dimethyl-, and...
Helix packing of lactose permease in Escherichia coli studied by site-directed chemical cleavage.
Wu, J, Perrin, D M, Sigman, D S, Kaback, H R
Biotinylated lactose permease from Escherichia coli containing a single-cysteine residue at position 330 (helix X) or at position 147, 148, or 149 (helix V) was purified by avidin-affinity...
Chen, C B, Gorin, M B, Sigman, D S
RNAs modified with the chemical nuclease 1,10-phenanthroline-copper(I) can achieve the sequence-specific scission of single- and double-stranded DNA targets. The RNAs are prepared in vitro by using...
A transcription inhibitor specific for unwound DNA in RNA polymerase-promoter open complexes.
Mazumder, A, Perrin, D M, Watson, K J, Sigman, D S
The kinetically component open complexes formed at prokaryotic and eukaryotic transcription start sites are efficiently nicked by the chemical nuclease activity of the 2:1...
Li, C, Lai, C F, Sigman, D S, Gaynor, R B
Human immunodeficiency virus (HIV) gene expression is regulated by both general transcription factors and factors induced by activation of T lymphocytes such as NF-kappa B and the nuclear factor of...
Kinetics of spontaneous displacement of RNA from heteroduplexes by DNA.
Landgraf, R, Ramamurthi, K S, Sigman, D S
We have used R-loop formation and direct hybridization techniques to analyze the kinetics by which RNA is displaced from a heteroduplex by DNA of identical sequence. Using random walk simulations we...
Gaynor, R B, Kuwabara, M D, Wu, F K, Garcia, J A, Harrich, D, Briskin, M, ...
The enhancer element of the human immunodeficiency virus type I (HIV-I) long terminal repeat (LTR) contains two copies of nearly identical sequences AGGGACTTTCC (3G sequence) and GGGGACTTTCC (4G...
Gaynor, R, Soultanakis, E, Kuwabara, M, Garcia, J, Sigman, D S
The transactivator protein, tat, encoded by the human immunodeficiency virus is a key regulator of viral transcription. Activation by the tat protein requires sequences downstream of the...
Law, R, Kuwabara, M D, Briskin, M, Fasel, N, Hermanson, G, Sigman, D S, ...
mRNAs specifying immunoglobulin mu and delta heavy chains are encoded by a single large, complex transcription unit (mu + delta gene). The transcriptional activity of delta gene segments in...
R-loop stability as a function of RNA structure and size.
Landgraf, R, Chen, C H, Sigman, D S
The sequence-specific formation of R-loops can be assayed using RNAs which overlap a HindIII cleavage site in a 3.5 kb plasmid. Chemical modification of the displaced DNA strand has permitted...
Double stranded scission of DNA directed through sequence-specific R-loop formation.
Landgraf, R, Chen, C H, Sigman, D S
R-loop formation with short (100 nt) RNAs provides a highly flexible and stringent method to achieve sequence-specific separation of target DNA at any given sequence. After stabilization of R-loops...
Murakawa, G J, Chen, C H, Kuwabara, M D, Nierlich, D P, Sigman, D S
The scission of RNA by the chemical nuclease activity of 1,10-phenanthroline-copper (OP-Cu) has been studied using a lac mRNA fragment and tRNAphe as substrates. Since the chemical mechanism of...
Young, A. P., Brown, F. F., Halsey, M. J., Sigman, D. S.
Incubation of membrane fragments bearing acetylcholine receptors from Torpedo californica under an atmosphere of 3% halothane, 1% chloroform, or 6% diethyl ether greatly facilitates the...
Secondary structure specificity of the nuclease activity of the 1,10-phenanthroline-copper complex.
The artificial DNase activity of the 1,10-phenanthroline-cuprous ion complex [(OP)2Cu+] and H2O2 cleaves the A, B, and Z forms of DNA at different rates. The B structure, formed by most DNAs...
Nuclease activity of 1,10-phenanthroline-copper: sequence-specific targeting.
The nuclease activity of 1,10-phenanthroline-copper ion can be targeted to specific DNA sequences by attachment of the ligand to the 5' end of complementary deoxyoligonucleotides via a...
Pearson, L, Chen, C B, Gaynor, R P, Sigman, D S
RNA-protein complexes isolated following a gel retardation assay can be footprinted within the gel matrix using the chemical nuclease activities of 4,7-dimethyl-, 5,6-dimethyl-, and...
Drosophila engrailed-1,10-phenanthroline chimeras as probes of homeodomain-DNA complexes.
Pan, C. Q., Landgraf, R., Sigman, D. S.
We have converted the Drosophila engrailed homeodomain into a sequence-specific nuclease by linking the protein to the chemical nuclease 1,10-phenanthroline-copper (OP-Cu). Unique cysteines were...